RapamycinFungusV1, Main, Corpus, bibRecord, 000129

Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.

Identifieur interne : 000129 ( Main/Corpus ); précédent : 000128; suivant : 000130

Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.

Auteurs : Yujie Li ; Hui Ren ; Yutao Zhao ; Jiyuan Sun ; Yanhua Fan ; Dan Jin ; Yan Pei

Source :

Journal of invertebrate pathology [ 1096-0805 ] ; 2020.

RBID : pubmed:32006551

Abstract

FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.

DOI: 10.1016/j.jip.2020.107334
PubMed: 32006551

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pubmed:32006551

Le document en format XML

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<author><name sortKey="Li, Yujie" sort="Li, Yujie" uniqKey="Li Y" first="Yujie" last="Li">Yujie Li</name>
<affiliation><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation>
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<author><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name>
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<author><name sortKey="Sun, Jiyuan" sort="Sun, Jiyuan" uniqKey="Sun J" first="Jiyuan" last="Sun">Jiyuan Sun</name>
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<author><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name>
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<front><div type="abstract" xml:lang="en">FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.</div>
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<Abstract><AbstractText>FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.</AbstractText>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Beauveria bassiana</Keyword>
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<Keyword MajorTopicYN="Y">Lovastatin</Keyword>
<Keyword MajorTopicYN="Y">Rapamycin</Keyword>
<Keyword MajorTopicYN="Y">Resistance</Keyword>
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<CoiStatement>Declaration of Competing Interests The authors declare that they have no competing interests.</CoiStatement>
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Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.

Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.

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Abstract

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