Reference gene selection for quantitative real-time polymerase chain reaction in Populus.
Identifieur interne : 002D34 ( Main/Exploration ); précédent : 002D33; suivant : 002D35Reference gene selection for quantitative real-time polymerase chain reaction in Populus.
Auteurs : Meng Xu [République populaire de Chine] ; Bo Zhang ; Xiaohua Su ; Shougong Zhang ; Minren HuangSource :
- Analytical biochemistry [ 1096-0309 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN ribosomique 18S (génétique), ARN ribosomique 18S (normes), Actines (génétique), Actines (normes), Facteur-1 d'élongation de la chaîne peptidique (génétique), Facteur-1 d'élongation de la chaîne peptidique (normes), Gènes de plante (MeSH), Normes de référence (MeSH), Populus (génétique), RT-PCR (méthodes), RT-PCR (normes), Séquence nucléotidique (MeSH).
- MESH :
- génétique : ARN ribosomique 18S, Actines, Facteur-1 d'élongation de la chaîne peptidique, Populus.
- méthodes : RT-PCR.
- normes : ARN ribosomique 18S, Actines, Facteur-1 d'élongation de la chaîne peptidique, RT-PCR.
- Gènes de plante, Normes de référence, Séquence nucléotidique.
English descriptors
- KwdEn :
- Actins (genetics), Actins (standards), Base Sequence (MeSH), Genes, Plant (MeSH), Peptide Elongation Factor 1 (genetics), Peptide Elongation Factor 1 (standards), Populus (genetics), RNA, Ribosomal, 18S (genetics), RNA, Ribosomal, 18S (standards), Reference Standards (MeSH), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards).
- MESH :
- chemical , genetics : Actins, Peptide Elongation Factor 1, RNA, Ribosomal, 18S.
- chemical , standards : Actins, Peptide Elongation Factor 1, RNA, Ribosomal, 18S.
- genetics : Populus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- Base Sequence, Genes, Plant, Reference Standards.
Abstract
Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.
DOI: 10.1016/j.ab.2010.08.044
PubMed: 20816740
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation>
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<author><name sortKey="Zhang, Shougong" sort="Zhang, Shougong" uniqKey="Zhang S" first="Shougong" last="Zhang">Shougong Zhang</name>
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<term>RNA, Ribosomal, 18S (standards)</term>
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<term>Actines (normes)</term>
<term>Facteur-1 d'élongation de la chaîne peptidique (génétique)</term>
<term>Facteur-1 d'élongation de la chaîne peptidique (normes)</term>
<term>Gènes de plante (MeSH)</term>
<term>Normes de référence (MeSH)</term>
<term>Populus (génétique)</term>
<term>RT-PCR (méthodes)</term>
<term>RT-PCR (normes)</term>
<term>Séquence nucléotidique (MeSH)</term>
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<front><div type="abstract" xml:lang="en">Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.</div>
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<Abstract><AbstractText>Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.</AbstractText>
<CopyrightInformation>Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.</CopyrightInformation>
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