Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system.
Identifieur interne : 003561 ( Main/Exploration ); précédent : 003560; suivant : 003562Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system.
Auteurs : Yimian Ma [République populaire de Chine] ; Luo Liu ; Chengguang Zhu ; Changhui Sun ; Bo Xu ; Jun Fang ; Jiuyou Tang ; Anding Luo ; Shouyun Cao ; Gupo Li ; Qian Qian ; Yongbiao Xue ; Chengcai ChuSource :
- Journal of genetics and genomics = Yi chuan xue bao [ 1673-8527 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Chromosomes de plante (génétique), Glucuronidase (génétique), Glucuronidase (métabolisme), Gènes rapporteurs (MeSH), Génome végétal (MeSH), Mutagenèse par insertion (MeSH), Oryza (croissance et développement), Oryza (génétique), Oryza (métabolisme), Rhizobium (génétique), Rhizobium (métabolisme), Transformation génétique (MeSH), Vecteurs génétiques (génétique).
- MESH :
- croissance et développement : Oryza.
- génétique : ADN bactérien, Chromosomes de plante, Glucuronidase, Oryza, Rhizobium, Vecteurs génétiques.
- métabolisme : Glucuronidase, Oryza, Rhizobium.
- Gènes rapporteurs, Génome végétal, Mutagenèse par insertion, Transformation génétique.
English descriptors
- KwdEn :
- Chromosomes, Plant (genetics), DNA, Bacterial (genetics), Genes, Reporter (MeSH), Genetic Vectors (genetics), Genome, Plant (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Mutagenesis, Insertional (MeSH), Oryza (genetics), Oryza (growth & development), Oryza (metabolism), Rhizobium (genetics), Rhizobium (metabolism), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA, Bacterial, Glucuronidase.
- genetics : Chromosomes, Plant, Genetic Vectors, Oryza, Rhizobium.
- growth & development : Oryza.
- chemical , metabolism : Glucuronidase, Oryza, Rhizobium.
- Genes, Reporter, Genome, Plant, Mutagenesis, Insertional, Transformation, Genetic.
Abstract
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
DOI: 10.1016/S1673-8527(08)60114-9
PubMed: 19447374
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>DNA, Bacterial (genetics)</term>
<term>Genes, Reporter (MeSH)</term>
<term>Genetic Vectors (genetics)</term>
<term>Genome, Plant (MeSH)</term>
<term>Glucuronidase (genetics)</term>
<term>Glucuronidase (metabolism)</term>
<term>Mutagenesis, Insertional (MeSH)</term>
<term>Oryza (genetics)</term>
<term>Oryza (growth & development)</term>
<term>Oryza (metabolism)</term>
<term>Rhizobium (genetics)</term>
<term>Rhizobium (metabolism)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term>
<term>Chromosomes de plante (génétique)</term>
<term>Glucuronidase (génétique)</term>
<term>Glucuronidase (métabolisme)</term>
<term>Gènes rapporteurs (MeSH)</term>
<term>Génome végétal (MeSH)</term>
<term>Mutagenèse par insertion (MeSH)</term>
<term>Oryza (croissance et développement)</term>
<term>Oryza (génétique)</term>
<term>Oryza (métabolisme)</term>
<term>Rhizobium (génétique)</term>
<term>Rhizobium (métabolisme)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term>
<term>Glucuronidase</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Oryza</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Plant</term>
<term>Genetic Vectors</term>
<term>Oryza</term>
<term>Rhizobium</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oryza</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term>
<term>Chromosomes de plante</term>
<term>Glucuronidase</term>
<term>Oryza</term>
<term>Rhizobium</term>
<term>Vecteurs génétiques</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term>
<term>Oryza</term>
<term>Rhizobium</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term>
<term>Oryza</term>
<term>Rhizobium</term>
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<keywords scheme="MESH" xml:lang="en"><term>Genes, Reporter</term>
<term>Genome, Plant</term>
<term>Mutagenesis, Insertional</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Gènes rapporteurs</term>
<term>Génome végétal</term>
<term>Mutagenèse par insertion</term>
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<front><div type="abstract" xml:lang="en">About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.</div>
</front>
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<Abstract><AbstractText>About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.</AbstractText>
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<Author ValidYN="Y"><LastName>Sun</LastName>
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