Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry.
Identifieur interne : 003952 ( Main/Exploration ); précédent : 003951; suivant : 003953Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry.
Auteurs : Ondrej Novák [République tchèque] ; Eva Hauserová ; Petra Amakorová ; Karel Dolezal ; Miroslav StrnadSource :
- Phytochemistry [ 0031-9422 ] ; 2008.
Descripteurs français
- KwdFr :
- Chromatographie en phase liquide à haute performance (méthodes), Cytokinine (composition chimique), Cytokinine (immunologie), Cytokinine (isolement et purification), Extraits de plantes (composition chimique), Extraits de plantes (immunologie), Facteurs temps (MeSH), Plantes (composition chimique), Spectrométrie de masse ESI (méthodes), Spectrométrie de masse en tandem (méthodes), Structure moléculaire (MeSH).
- MESH :
- composition chimique : Cytokinine, Extraits de plantes, Plantes.
- immunologie : Cytokinine, Extraits de plantes.
- isolement et purification : Cytokinine.
- méthodes : Chromatographie en phase liquide à haute performance, Spectrométrie de masse ESI, Spectrométrie de masse en tandem.
- Facteurs temps, Structure moléculaire.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (methods), Cytokinins (chemistry), Cytokinins (immunology), Cytokinins (isolation & purification), Molecular Structure (MeSH), Plant Extracts (chemistry), Plant Extracts (immunology), Plants (chemistry), Spectrometry, Mass, Electrospray Ionization (methods), Tandem Mass Spectrometry (methods), Time Factors (MeSH).
- MESH :
- chemical , chemistry : Cytokinins, Plant Extracts.
- chemical , immunology : Cytokinins, Plant Extracts.
- chemical , isolation & purification : Cytokinins.
- chemistry : Plants.
- methods : Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry.
- Molecular Structure, Time Factors.
Abstract
We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15-0.3% and 1.0-5.5%, respectively). In multiple reaction monitoring (MRM) mode, the detection limit for most of the cytokinins analyzed was close to 1 fmol (5-25 fmol for O-glucosides and nucleotides) and the linear range spanned at least five orders of magnitude. The extraction and purification method was optimized using poplar (Populusxcanadensis Moench, cv Robusta) leaf samples, and the analytical accuracy was further validated using IAE-purified 10-day-old Arabidopsis thaliana plants spiked with 1 and 10 pmol of cytokinin derivatives. This approach can be used for rapid, sensitive qualitative and/or quantitative analysis of more than 50 natural cytokinins in minute amounts of plant tissues with high performance, robustness, and accuracy.
DOI: 10.1016/j.phytochem.2008.04.022
PubMed: 18561963
Affiliations:
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Hauserová</name></author><author><name sortKey="Amakorova, Petra" sort="Amakorova, Petra" uniqKey="Amakorova P" first="Petra" last="Amakorová">Petra Amakorová</name></author><author><name sortKey="Dolezal, Karel" sort="Dolezal, Karel" uniqKey="Dolezal K" first="Karel" last="Dolezal">Karel Dolezal</name></author><author><name sortKey="Strnad, Miroslav" sort="Strnad, Miroslav" uniqKey="Strnad M" first="Miroslav" last="Strnad">Miroslav Strnad</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18561963</idno><idno type="pmid">18561963</idno><idno type="doi">10.1016/j.phytochem.2008.04.022</idno><idno type="wicri:Area/Main/Corpus">003858</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003858</idno><idno type="wicri:Area/Main/Curation">003858</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003858</idno><idno 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first="Eva" last="Hauserová">Eva Hauserová</name></author><author><name sortKey="Amakorova, Petra" sort="Amakorova, Petra" uniqKey="Amakorova P" first="Petra" last="Amakorová">Petra Amakorová</name></author><author><name sortKey="Dolezal, Karel" sort="Dolezal, Karel" uniqKey="Dolezal K" first="Karel" last="Dolezal">Karel Dolezal</name></author><author><name sortKey="Strnad, Miroslav" sort="Strnad, Miroslav" uniqKey="Strnad M" first="Miroslav" last="Strnad">Miroslav Strnad</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="ISSN">0031-9422</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid (methods)</term><term>Cytokinins (chemistry)</term><term>Cytokinins (immunology)</term><term>Cytokinins (isolation & purification)</term><term>Molecular Structure (MeSH)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (immunology)</term><term>Plants (chemistry)</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term><term>Tandem Mass Spectrometry (methods)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Cytokinine (composition chimique)</term><term>Cytokinine (immunologie)</term><term>Cytokinine (isolement et purification)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (immunologie)</term><term>Facteurs temps (MeSH)</term><term>Plantes (composition chimique)</term><term>Spectrométrie de masse ESI (méthodes)</term><term>Spectrométrie de masse en tandem (méthodes)</term><term>Structure moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cytokinins</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Cytokinins</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Cytokinins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cytokinine</term><term>Extraits de plantes</term><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cytokinine</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Cytokinine</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Spectrométrie de masse ESI</term><term>Spectrométrie de masse en tandem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Structure</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Structure moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15-0.3% and 1.0-5.5%, respectively). In multiple reaction monitoring (MRM) mode, the detection limit for most of the cytokinins analyzed was close to 1 fmol (5-25 fmol for O-glucosides and nucleotides) and the linear range spanned at least five orders of magnitude. The extraction and purification method was optimized using poplar (Populusxcanadensis Moench, cv Robusta) leaf samples, and the analytical accuracy was further validated using IAE-purified 10-day-old Arabidopsis thaliana plants spiked with 1 and 10 pmol of cytokinin derivatives. This approach can be used for rapid, sensitive qualitative and/or quantitative analysis of more than 50 natural cytokinins in minute amounts of plant tissues with high performance, robustness, and accuracy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18561963</PMID><DateCompleted><Year>2008</Year><Month>09</Month><Day>17</Day></DateCompleted><DateRevised><Year>2008</Year><Month>08</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>69</Volume><Issue>11</Issue><PubDate><Year>2008</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>2214-24</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2008.04.022</ELocationID><Abstract><AbstractText>We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15-0.3% and 1.0-5.5%, respectively). In multiple reaction monitoring (MRM) mode, the detection limit for most of the cytokinins analyzed was close to 1 fmol (5-25 fmol for O-glucosides and nucleotides) and the linear range spanned at least five orders of magnitude. The extraction and purification method was optimized using poplar (Populusxcanadensis Moench, cv Robusta) leaf samples, and the analytical accuracy was further validated using IAE-purified 10-day-old Arabidopsis thaliana plants spiked with 1 and 10 pmol of cytokinin derivatives. This approach can be used for rapid, sensitive qualitative and/or quantitative analysis of more than 50 natural cytokinins in minute amounts of plant tissues with high performance, robustness, and accuracy.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Novák</LastName><ForeName>Ondrej</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Laboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany ASCR, Slechtitelů 11, CZ-78371 Olomouc, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hauserová</LastName><ForeName>Eva</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Amakorová</LastName><ForeName>Petra</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Dolezal</LastName><ForeName>Karel</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Strnad</LastName><ForeName>Miroslav</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>06</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003583">Cytokinins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003583" MajorTopicYN="N">Cytokinins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>02</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>04</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>9</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18561963</ArticleId><ArticleId IdType="pii">S0031-9422(08)00218-5</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2008.04.022</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Amakorova, Petra" sort="Amakorova, Petra" uniqKey="Amakorova P" first="Petra" last="Amakorová">Petra Amakorová</name><name sortKey="Dolezal, Karel" sort="Dolezal, Karel" uniqKey="Dolezal K" first="Karel" last="Dolezal">Karel Dolezal</name><name sortKey="Hauserova, Eva" sort="Hauserova, Eva" uniqKey="Hauserova E" first="Eva" last="Hauserová">Eva Hauserová</name><name sortKey="Strnad, Miroslav" sort="Strnad, Miroslav" uniqKey="Strnad M" first="Miroslav" last="Strnad">Miroslav Strnad</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Novak, Ondrej" sort="Novak, Ondrej" uniqKey="Novak O" first="Ondrej" last="Novák">Ondrej Novák</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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