Immunodetection and Identification of N-(o-Hydroxybenzylamino)Purine as a Naturally Occurring Cytokinin in Populus x canadensis Moench cv Robusta Leaves.
Identifieur interne : 004C19 ( Main/Exploration ); précédent : 004C18; suivant : 004C20Immunodetection and Identification of N-(o-Hydroxybenzylamino)Purine as a Naturally Occurring Cytokinin in Populus x canadensis Moench cv Robusta Leaves.
Auteurs : M. Strnad [Tchécoslovaquie] ; W. Peters ; E. Beck ; M. KamínekSource :
- Plant physiology [ 0032-0889 ] ; 1992.
Abstract
A highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for 9-beta-d-ribofuranosyl-N(6)-(o-hydroxybenzylamino)purine [(oOH)[9R]BAP] and structurally related cytokinins. As little as 3 femtomoles of the compound could be detected by this method. Cross-reactivity studies demonstrated the specificity of four polyclonal antibodies for (oOH)[9R] BAP and its free base in preference to a range of natural cytokinins and other purines. After evaluating the method by internal standardization employing [2-(3)H](oOH)[9R]BAP of high specific radioactivity as recovery marker by dilution analyses and by immunohistograms, it was possible to apply ELISA to quantify (oOH)[9R]BAP in plant extracts. In addition to (oOH)[9R]BAP, an unknown cytokinin reacting with the same antibody was detected in high performance liquid chromatography-fractionated extracts of mature Populus x canadensis Moench cv Robusta. The structure of the new compound was determined by gas chromatography-mass spectrometry and finally confirmed by synthesis as N(6)-(o-hydroxybenzylamino)purine.
DOI: 10.1104/pp.99.1.74
PubMed: 16668886
PubMed Central: PMC1080408
Affiliations:
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Strnad</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biotechnology, Institute of Experimental Botany, Sokolovská 6, 772 00 Olomouc, Czechoslovakia.</nlm:affiliation><country xml:lang="fr">Tchécoslovaquie</country><wicri:regionArea>Department of Plant Biotechnology, Institute of Experimental Botany, Sokolovská 6, 772 00 Olomouc</wicri:regionArea><wicri:noRegion>772 00 Olomouc</wicri:noRegion></affiliation></author><author><name sortKey="Peters, W" sort="Peters, W" uniqKey="Peters W" first="W" last="Peters">W. Peters</name></author><author><name sortKey="Beck, E" sort="Beck, E" uniqKey="Beck E" first="E" last="Beck">E. Beck</name></author><author><name sortKey="Kaminek, M" sort="Kaminek, M" uniqKey="Kaminek M" first="M" last="Kamínek">M. Kamínek</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for 9-beta-d-ribofuranosyl-N(6)-(o-hydroxybenzylamino)purine [(oOH)[9R]BAP] and structurally related cytokinins. As little as 3 femtomoles of the compound could be detected by this method. Cross-reactivity studies demonstrated the specificity of four polyclonal antibodies for (oOH)[9R] BAP and its free base in preference to a range of natural cytokinins and other purines. After evaluating the method by internal standardization employing [2-(3)H](oOH)[9R]BAP of high specific radioactivity as recovery marker by dilution analyses and by immunohistograms, it was possible to apply ELISA to quantify (oOH)[9R]BAP in plant extracts. In addition to (oOH)[9R]BAP, an unknown cytokinin reacting with the same antibody was detected in high performance liquid chromatography-fractionated extracts of mature Populus x canadensis Moench cv Robusta. 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As little as 3 femtomoles of the compound could be detected by this method. Cross-reactivity studies demonstrated the specificity of four polyclonal antibodies for (oOH)[9R] BAP and its free base in preference to a range of natural cytokinins and other purines. After evaluating the method by internal standardization employing [2-(3)H](oOH)[9R]BAP of high specific radioactivity as recovery marker by dilution analyses and by immunohistograms, it was possible to apply ELISA to quantify (oOH)[9R]BAP in plant extracts. In addition to (oOH)[9R]BAP, an unknown cytokinin reacting with the same antibody was detected in high performance liquid chromatography-fractionated extracts of mature Populus x canadensis Moench cv Robusta. The structure of the new compound was determined by gas chromatography-mass spectrometry and finally confirmed by synthesis as N(6)-(o-hydroxybenzylamino)purine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Strnad</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Biotechnology, Institute of Experimental Botany, Sokolovská 6, 772 00 Olomouc, Czechoslovakia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peters</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Beck</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Kamínek</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16668886</ArticleId><ArticleId IdType="pmc">PMC1080408</ArticleId><ArticleId IdType="doi">10.1104/pp.99.1.74</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Klin Wochenschr. 1987 Feb 2;65(3):113-20</Citation><ArticleIdList><ArticleId IdType="pubmed">3573679</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1980 Aug 29;95(4):1387-92</Citation><ArticleIdList><ArticleId IdType="pubmed">7417323</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1984 Aug;75(4):1117-25</Citation><ArticleIdList><ArticleId IdType="pubmed">16663745</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1986 Jun;81(2):516-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16664848</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1987 Jul;84(3):571-3</Citation><ArticleIdList><ArticleId IdType="pubmed">16665481</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Tchécoslovaquie</li></country></list><tree><noCountry><name sortKey="Beck, E" sort="Beck, E" uniqKey="Beck E" first="E" last="Beck">E. Beck</name><name sortKey="Kaminek, M" sort="Kaminek, M" uniqKey="Kaminek M" first="M" last="Kamínek">M. Kamínek</name><name sortKey="Peters, W" sort="Peters, W" uniqKey="Peters W" first="W" last="Peters">W. Peters</name></noCountry><country name="Tchécoslovaquie"><noRegion><name sortKey="Strnad, M" sort="Strnad, M" uniqKey="Strnad M" first="M" last="Strnad">M. Strnad</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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