An enzyme-linked immunosorbent assay (ELISA) for detection of Clostridium aldrichii in anaerobiodigesters.
Identifieur interne : 004B54 ( Main/Exploration ); précédent : 004B53; suivant : 004B55An enzyme-linked immunosorbent assay (ELISA) for detection of Clostridium aldrichii in anaerobiodigesters.
Auteurs : R L Brigmon ; D P Chynoweth ; J C Yang ; S G ZamSource :
- The Journal of applied bacteriology [ 0021-8847 ] ; 1994.
Descripteurs français
- KwdFr :
- Anaérobiose (MeSH), Animaux (MeSH), Anticorps antibactériens (MeSH), Anticorps monoclonaux (MeSH), Antigènes bactériens (analyse), Clostridium (immunologie), Clostridium (isolement et purification), Sensibilité et spécificité (MeSH), Souris (MeSH), Souris de lignée BALB C (MeSH), Spécificité des anticorps (MeSH), Techniques bactériologiques (statistiques et données numériques), Test ELISA (méthodes), Test ELISA (statistiques et données numériques), Épitopes (analyse), Études d'évaluation comme sujet (MeSH).
- MESH :
- analyse : Antigènes bactériens, Épitopes.
- immunologie : Clostridium.
- isolement et purification : Clostridium.
- méthodes : Test ELISA.
- statistiques et données numériques : Techniques bactériologiques, Test ELISA.
- Anaérobiose, Animaux, Anticorps antibactériens, Anticorps monoclonaux, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Spécificité des anticorps, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Anaerobiosis (MeSH), Animals (MeSH), Antibodies, Bacterial (MeSH), Antibodies, Monoclonal (MeSH), Antibody Specificity (MeSH), Antigens, Bacterial (analysis), Bacteriological Techniques (statistics & numerical data), Clostridium (immunology), Clostridium (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), Epitopes (analysis), Evaluation Studies as Topic (MeSH), Mice (MeSH), Mice, Inbred BALB C (MeSH), Sensitivity and Specificity (MeSH).
- MESH :
- chemical , analysis : Antigens, Bacterial, Epitopes.
- chemical : Antibodies, Bacterial, Antibodies, Monoclonal.
- immunology : Clostridium.
- isolation & purification : Clostridium.
- methods : Enzyme-Linked Immunosorbent Assay.
- statistics & numerical data : Bacteriological Techniques, Enzyme-Linked Immunosorbent Assay.
- Anaerobiosis, Animals, Antibody Specificity, Evaluation Studies as Topic, Mice, Mice, Inbred BALB C, Sensitivity and Specificity.
Abstract
Monoclonal antibodies (Mabs) against Clostridium aldrichii were prepared by in vivo and in vitro immunization with whole cells and produced after fusion as ascites in BALB/c mice. An enzyme-linked immunosorbent assay (ELISA) was used to test for specificity and sensitivity of the Mabs to detect Cl. aldrichii. The lower limit for Cl. aldrichii detection in pure and mixed culture with ELISA was 10(5) cells ml-1. Twenty other species of bacteria, including 12 cellulolytic species, were tested for cross-reactivity with the ELISA, but none was detected. The ELISA was used for detection of Cl. aldrichii over a 16-month period in five mesophilic continuously-stirred tank reactors (CSTR) with wood, glucose, sludge or sorghum as substrates. The population of Cl. aldrichii in the poplar wood anaerobic digester effluent was 10(6)-10(7) cells ml-1 over that time. These numbers were confirmed by anaerobic microbiological methods. Results from the ELISA technique were obtained in 36 h vs 3 weeks for culture methods. It is concluded that the ELISA is a useful, time-saving method for identification, detection and quantification of Cl. aldrichii in axenic, mixed culture, and in complex undefined cultures such as those found in anaerobic digesters.
DOI: 10.1111/j.1365-2672.1994.tb03448.x
PubMed: 7527384
Affiliations:
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Le document en format XML
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code="subField">Gainesville</wicri:noCountry></affiliation></author><author><name sortKey="Chynoweth, D P" sort="Chynoweth, D P" uniqKey="Chynoweth D" first="D P" last="Chynoweth">D P Chynoweth</name></author><author><name sortKey="Yang, J C" sort="Yang, J C" uniqKey="Yang J" first="J C" last="Yang">J C Yang</name></author><author><name sortKey="Zam, S G" sort="Zam, S G" uniqKey="Zam S" first="S G" last="Zam">S G Zam</name></author></analytic><series><title level="j">The Journal of applied bacteriology</title><idno type="ISSN">0021-8847</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anaerobiosis (MeSH)</term><term>Animals (MeSH)</term><term>Antibodies, Bacterial (MeSH)</term><term>Antibodies, Monoclonal (MeSH)</term><term>Antibody Specificity (MeSH)</term><term>Antigens, Bacterial (analysis)</term><term>Bacteriological Techniques (statistics & numerical data)</term><term>Clostridium (immunology)</term><term>Clostridium (isolation & purification)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term><term>Epitopes (analysis)</term><term>Evaluation Studies as Topic (MeSH)</term><term>Mice (MeSH)</term><term>Mice, Inbred BALB C (MeSH)</term><term>Sensitivity and Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anaérobiose (MeSH)</term><term>Animaux (MeSH)</term><term>Anticorps antibactériens (MeSH)</term><term>Anticorps monoclonaux (MeSH)</term><term>Antigènes bactériens (analyse)</term><term>Clostridium (immunologie)</term><term>Clostridium (isolement et purification)</term><term>Sensibilité et spécificité (MeSH)</term><term>Souris (MeSH)</term><term>Souris de lignée BALB C (MeSH)</term><term>Spécificité des anticorps (MeSH)</term><term>Techniques bactériologiques (statistiques et données numériques)</term><term>Test ELISA (méthodes)</term><term>Test ELISA (statistiques et données numériques)</term><term>Épitopes (analyse)</term><term>Études d'évaluation comme sujet (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Bacterial</term><term>Epitopes</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Bacterial</term><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes bactériens</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Clostridium</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Clostridium</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Clostridium</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Clostridium</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Test ELISA</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Bacteriological Techniques</term><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="statistiques et données numériques" xml:lang="fr"><term>Techniques bactériologiques</term><term>Test ELISA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Anaerobiosis</term><term>Animals</term><term>Antibody Specificity</term><term>Evaluation Studies as Topic</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anaérobiose</term><term>Animaux</term><term>Anticorps antibactériens</term><term>Anticorps monoclonaux</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Études d'évaluation comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monoclonal antibodies (Mabs) against Clostridium aldrichii were prepared by in vivo and in vitro immunization with whole cells and produced after fusion as ascites in BALB/c mice. An enzyme-linked immunosorbent assay (ELISA) was used to test for specificity and sensitivity of the Mabs to detect Cl. aldrichii. The lower limit for Cl. aldrichii detection in pure and mixed culture with ELISA was 10(5) cells ml-1. Twenty other species of bacteria, including 12 cellulolytic species, were tested for cross-reactivity with the ELISA, but none was detected. The ELISA was used for detection of Cl. aldrichii over a 16-month period in five mesophilic continuously-stirred tank reactors (CSTR) with wood, glucose, sludge or sorghum as substrates. The population of Cl. aldrichii in the poplar wood anaerobic digester effluent was 10(6)-10(7) cells ml-1 over that time. These numbers were confirmed by anaerobic microbiological methods. Results from the ELISA technique were obtained in 36 h vs 3 weeks for culture methods. It is concluded that the ELISA is a useful, time-saving method for identification, detection and quantification of Cl. aldrichii in axenic, mixed culture, and in complex undefined cultures such as those found in anaerobic digesters.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7527384</PMID><DateCompleted><Year>1995</Year><Month>01</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-8847</ISSN><JournalIssue CitedMedium="Print"><Volume>77</Volume><Issue>4</Issue><PubDate><Year>1994</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The Journal of applied bacteriology</Title><ISOAbbreviation>J Appl Bacteriol</ISOAbbreviation></Journal><ArticleTitle>An enzyme-linked immunosorbent assay (ELISA) for detection of Clostridium aldrichii in anaerobiodigesters.</ArticleTitle><Pagination><MedlinePgn>448-55</MedlinePgn></Pagination><Abstract><AbstractText>Monoclonal antibodies (Mabs) against Clostridium aldrichii were prepared by in vivo and in vitro immunization with whole cells and produced after fusion as ascites in BALB/c mice. An enzyme-linked immunosorbent assay (ELISA) was used to test for specificity and sensitivity of the Mabs to detect Cl. aldrichii. The lower limit for Cl. aldrichii detection in pure and mixed culture with ELISA was 10(5) cells ml-1. Twenty other species of bacteria, including 12 cellulolytic species, were tested for cross-reactivity with the ELISA, but none was detected. The ELISA was used for detection of Cl. aldrichii over a 16-month period in five mesophilic continuously-stirred tank reactors (CSTR) with wood, glucose, sludge or sorghum as substrates. The population of Cl. aldrichii in the poplar wood anaerobic digester effluent was 10(6)-10(7) cells ml-1 over that time. These numbers were confirmed by anaerobic microbiological methods. Results from the ELISA technique were obtained in 36 h vs 3 weeks for culture methods. It is concluded that the ELISA is a useful, time-saving method for identification, detection and quantification of Cl. aldrichii in axenic, mixed culture, and in complex undefined cultures such as those found in anaerobic digesters.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brigmon</LastName><ForeName>R L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Environmental Engineering Sciences, University of Florida, Gainesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chynoweth</LastName><ForeName>D P</ForeName><Initials>DP</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Zam</LastName><ForeName>S G</ForeName><Initials>SG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Bacteriol</MedlineTA><NlmUniqueID>7503050</NlmUniqueID><ISSNLinking>0021-8847</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000907">Antibodies, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000942">Antigens, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000693" MajorTopicYN="N">Anaerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000907" MajorTopicYN="N">Antibodies, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000942" MajorTopicYN="N">Antigens, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001431" MajorTopicYN="Y">Bacteriological Techniques</DescriptorName><QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003013" MajorTopicYN="N">Clostridium</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName><QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005069" MajorTopicYN="N">Evaluation Studies as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1994</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1994</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7527384</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2672.1994.tb03448.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Brigmon, R L" sort="Brigmon, R L" uniqKey="Brigmon R" first="R L" last="Brigmon">R L Brigmon</name><name sortKey="Chynoweth, D P" sort="Chynoweth, D P" uniqKey="Chynoweth D" first="D P" last="Chynoweth">D P Chynoweth</name><name sortKey="Yang, J C" sort="Yang, J C" uniqKey="Yang J" first="J C" last="Yang">J C Yang</name><name sortKey="Zam, S G" sort="Zam, S G" uniqKey="Zam S" first="S G" last="Zam">S G Zam</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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