Efficient plantlet regeneration from protoplasts isolated from suspension cultures of poplar (Populus alba L.).
Identifieur interne : 004947 ( Main/Exploration ); précédent : 004946; suivant : 004948Efficient plantlet regeneration from protoplasts isolated from suspension cultures of poplar (Populus alba L.).
Auteurs : J. Qiao [Japon] ; H. Kuroda [Japon] ; T. Hayashi [Japon] ; F. Sakai [Japon]Source :
- Plant cell reports [ 1432-203X ] ; 1998.
Abstract
We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Murashige and Skoog (MS) medium supplemented with 5 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.05 µM thidiazuron (TDZ) and 0.5 M glucose as a osmoticum. The plating efficiency of the cultured protoplasts was calculated at 26.5% at day 7 and 31.7% at day 14. Cell colonies were observed after culturing for 4 weeks. Regenerated colonies were propagated through subculture in liquid MS medium supplemented with 5 µM 2,4-D. Buds were induced from regenerated calli on MS medium containing 10 µM kinetin or 1 µM TDZ. Regenerated shoots were rooted on half-strength MS medium, and the plantlets were transplanted in soil. Randomly amplified polymorphic DNA analysis did not detect any DNA polymorphism among the regenerated plants.
DOI: 10.1007/s002990050378
PubMed: 30736500
Affiliations:
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Kuroda</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Sakai, F" sort="Sakai, F" uniqKey="Sakai F" first="F" last="Sakai">F. 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Qiao</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kuroda, H" sort="Kuroda, H" uniqKey="Kuroda H" first="H" last="Kuroda">H. Kuroda</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Sakai, F" sort="Sakai, F" uniqKey="Sakai F" first="F" last="Sakai">F. Sakai</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</nlm:affiliation><orgName type="university">Université de Kyoto</orgName><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 10<sup>6</sup> cells/g fresh weight cells and then cultured at a concentration of 2.5×10<sup>5</sup> cells/ml in NH<sub>4</sub>NO<sub>3</sub>-free Murashige and Skoog (MS) medium supplemented with 5 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.05 µM thidiazuron (TDZ) and 0.5 M glucose as a osmoticum. The plating efficiency of the cultured protoplasts was calculated at 26.5% at day 7 and 31.7% at day 14. Cell colonies were observed after culturing for 4 weeks. Regenerated colonies were propagated through subculture in liquid MS medium supplemented with 5 µM 2,4-D. Buds were induced from regenerated calli on MS medium containing 10 µM kinetin or 1 µM TDZ. Regenerated shoots were rooted on half-strength MS medium, and the plantlets were transplanted in soil. Randomly amplified polymorphic DNA analysis did not detect any DNA polymorphism among the regenerated plants.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30736500</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>17</Volume><Issue>3</Issue><PubDate><Year>1998</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Efficient plantlet regeneration from protoplasts isolated from suspension cultures of poplar (Populus alba L.).</ArticleTitle><Pagination><MedlinePgn>201-205</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s002990050378</ELocationID><Abstract><AbstractText>We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 10<sup>6</sup> cells/g fresh weight cells and then cultured at a concentration of 2.5×10<sup>5</sup> cells/ml in NH<sub>4</sub>NO<sub>3</sub>-free Murashige and Skoog (MS) medium supplemented with 5 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.05 µM thidiazuron (TDZ) and 0.5 M glucose as a osmoticum. The plating efficiency of the cultured protoplasts was calculated at 26.5% at day 7 and 31.7% at day 14. Cell colonies were observed after culturing for 4 weeks. Regenerated colonies were propagated through subculture in liquid MS medium supplemented with 5 µM 2,4-D. Buds were induced from regenerated calli on MS medium containing 10 µM kinetin or 1 µM TDZ. Regenerated shoots were rooted on half-strength MS medium, and the plantlets were transplanted in soil. Randomly amplified polymorphic DNA analysis did not detect any DNA polymorphism among the regenerated plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qiao</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuroda</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hayashi</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sakai</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Wood Research Institute, Kyoto University, Uji, Kyoto, 611 Japan, , , , , , JP.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Key wordsPopulus alba L.</Keyword><Keyword MajorTopicYN="N">Plant regeneration</Keyword><Keyword MajorTopicYN="N">Protoplast</Keyword><Keyword MajorTopicYN="N">RAPD</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30736500</ArticleId><ArticleId IdType="doi">10.1007/s002990050378</ArticleId><ArticleId IdType="pii">10.1007/s002990050378</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Qiao, J" sort="Qiao, J" uniqKey="Qiao J" first="J" last="Qiao">J. Qiao</name></region><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name><name sortKey="Kuroda, H" sort="Kuroda, H" uniqKey="Kuroda H" first="H" last="Kuroda">H. Kuroda</name><name sortKey="Sakai, F" sort="Sakai, F" uniqKey="Sakai F" first="F" last="Sakai">F. Sakai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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