A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress.
Identifieur interne : 004839 ( Main/Exploration ); précédent : 004838; suivant : 004840A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress.
Auteurs : L. Wu [États-Unis] ; C P Joshi ; V L ChiangSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2000.
Descripteurs français
- KwdFr :
- Arbres (génétique), Arbres (métabolisme), Cellulose (biosynthèse), Cellulose (métabolisme), Clonage moléculaire (MeSH), Contrainte mécanique (MeSH), Glucosyltransferases (génétique), Glucosyltransferases (métabolisme), Gènes rapporteurs (MeSH), Hybridation in situ (MeSH), Paroi cellulaire (métabolisme), Protéines d'Arabidopsis (MeSH), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Structures de plante (croissance et développement), Structures de plante (métabolisme), Tabac (génétique), Tabac (métabolisme), Technique de Northern (MeSH), Technique de Southern (MeSH), Végétaux génétiquement modifiés (MeSH), Végétaux toxiques (MeSH).
- MESH :
- biosynthèse : Cellulose.
- croissance et développement : Structures de plante.
- génétique : Arbres, Glucosyltransferases, Tabac.
- métabolisme : Arbres, Cellulose, Glucosyltransferases, Paroi cellulaire, Structures de plante, Tabac.
- Clonage moléculaire, Contrainte mécanique, Gènes rapporteurs, Hybridation in situ, Protéines d'Arabidopsis, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Technique de Northern, Technique de Southern, Végétaux génétiquement modifiés, Végétaux toxiques.
English descriptors
- KwdEn :
- Arabidopsis Proteins (MeSH), Blotting, Northern (MeSH), Blotting, Southern (MeSH), Cell Wall (metabolism), Cellulose (biosynthesis), Cellulose (metabolism), Cloning, Molecular (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Reporter (MeSH), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), In Situ Hybridization (MeSH), Plant Structures (growth & development), Plant Structures (metabolism), Plants, Genetically Modified (MeSH), Plants, Toxic (MeSH), Promoter Regions, Genetic (MeSH), Stress, Mechanical (MeSH), Tobacco (genetics), Tobacco (metabolism), Trees (genetics), Trees (metabolism).
- MESH :
- chemical , biosynthesis : Cellulose.
- chemical , genetics : Glucosyltransferases.
- chemical , metabolism : Cellulose, Glucosyltransferases.
- chemical : Arabidopsis Proteins.
- genetics : Tobacco, Trees.
- growth & development : Plant Structures.
- metabolism : Cell Wall, Plant Structures, Tobacco, Trees.
- Blotting, Northern, Blotting, Southern, Cloning, Molecular, Gene Expression Regulation, Plant, Genes, Reporter, In Situ Hybridization, Plants, Genetically Modified, Plants, Toxic, Promoter Regions, Genetic, Stress, Mechanical.
Abstract
Angiosperm trees accumulate an elevated amount of highly crystalline cellulose with a concomitant decrease in lignin in the cell walls of tension-stressed tissues. To investigate the molecular basis of this tree stress response, we cloned a full-length cellulose synthase (PtCesA) cDNA from developing xylem of aspen (Populus tremuloides). About 90% sequence similarity was found between the predicted PtCesA and cotton GhCesA proteins. Northern blot and in situ hybridization analyses of PtCesA gene transcripts in various aspen tissues, and PtCesA gene promoter-beta-glucuronidase (GUS) fusion analysis in transgenic tobacco, demonstrated conclusively that PtCesA expression is confined to developing xylem cells during normal plant growth. During mechanical stress induced by stem bending, GUS expression remained in xylem and was induced in developing phloem fibers undergoing tension stress, but was turned off in tissues undergoing compression on the opposite side of the bend. Our results suggest a unique role for PtCesA in cellulose biosynthesis in both tension-stressed and normal tissues in aspen, and that the on/off control of PtCesA expression may be a part of a signaling mechanism triggering a stress-related compensatory deposition of cellulose and lignin that is crucial to growth and development in trees.
DOI: 10.1046/j.1365-313x.2000.00758.x
PubMed: 10886769
Affiliations:
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Wu</name><affiliation wicri:level="1"><nlm:affiliation>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton 49931, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton 49931</wicri:regionArea><wicri:noRegion>Houghton 49931</wicri:noRegion></affiliation></author><author><name sortKey="Joshi, C P" sort="Joshi, C P" uniqKey="Joshi C" first="C P" last="Joshi">C P Joshi</name></author><author><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10886769</idno><idno type="pmid">10886769</idno><idno type="doi">10.1046/j.1365-313x.2000.00758.x</idno><idno type="wicri:Area/Main/Corpus">004849</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004849</idno><idno type="wicri:Area/Main/Curation">004849</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004849</idno><idno type="wicri:Area/Main/Exploration">004849</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress.</title><author><name sortKey="Wu, L" sort="Wu, L" uniqKey="Wu L" first="L" last="Wu">L. Wu</name><affiliation wicri:level="1"><nlm:affiliation>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton 49931, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton 49931</wicri:regionArea><wicri:noRegion>Houghton 49931</wicri:noRegion></affiliation></author><author><name sortKey="Joshi, C P" sort="Joshi, C P" uniqKey="Joshi C" first="C P" last="Joshi">C P Joshi</name></author><author><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="ISSN">0960-7412</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis Proteins (MeSH)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cellulose (biosynthesis)</term><term>Cellulose (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Glucosyltransferases (genetics)</term><term>Glucosyltransferases (metabolism)</term><term>In Situ Hybridization (MeSH)</term><term>Plant Structures (growth & development)</term><term>Plant Structures (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Plants, Toxic (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Stress, Mechanical (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Trees (genetics)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Cellulose (biosynthèse)</term><term>Cellulose (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Contrainte mécanique (MeSH)</term><term>Glucosyltransferases (génétique)</term><term>Glucosyltransferases (métabolisme)</term><term>Gènes rapporteurs (MeSH)</term><term>Hybridation in situ (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Protéines d'Arabidopsis (MeSH)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Structures de plante (croissance et développement)</term><term>Structures de plante (métabolisme)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Technique de Northern (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Végétaux toxiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Arabidopsis Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Structures de plante</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Structures</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Glucosyltransferases</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant Structures</term><term>Tobacco</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Cellulose</term><term>Glucosyltransferases</term><term>Paroi cellulaire</term><term>Structures de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Blotting, Southern</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Plant</term><term>Genes, Reporter</term><term>In Situ Hybridization</term><term>Plants, Genetically Modified</term><term>Plants, Toxic</term><term>Promoter Regions, Genetic</term><term>Stress, Mechanical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Contrainte mécanique</term><term>Gènes rapporteurs</term><term>Hybridation in situ</term><term>Protéines d'Arabidopsis</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Technique de Northern</term><term>Technique de Southern</term><term>Végétaux génétiquement modifiés</term><term>Végétaux toxiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Angiosperm trees accumulate an elevated amount of highly crystalline cellulose with a concomitant decrease in lignin in the cell walls of tension-stressed tissues. To investigate the molecular basis of this tree stress response, we cloned a full-length cellulose synthase (PtCesA) cDNA from developing xylem of aspen (Populus tremuloides). About 90% sequence similarity was found between the predicted PtCesA and cotton GhCesA proteins. Northern blot and in situ hybridization analyses of PtCesA gene transcripts in various aspen tissues, and PtCesA gene promoter-beta-glucuronidase (GUS) fusion analysis in transgenic tobacco, demonstrated conclusively that PtCesA expression is confined to developing xylem cells during normal plant growth. During mechanical stress induced by stem bending, GUS expression remained in xylem and was induced in developing phloem fibers undergoing tension stress, but was turned off in tissues undergoing compression on the opposite side of the bend. Our results suggest a unique role for PtCesA in cellulose biosynthesis in both tension-stressed and normal tissues in aspen, and that the on/off control of PtCesA expression may be a part of a signaling mechanism triggering a stress-related compensatory deposition of cellulose and lignin that is crucial to growth and development in trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10886769</PMID><DateCompleted><Year>2000</Year><Month>09</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0960-7412</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>6</Issue><PubDate><Year>2000</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress.</ArticleTitle><Pagination><MedlinePgn>495-502</MedlinePgn></Pagination><Abstract><AbstractText>Angiosperm trees accumulate an elevated amount of highly crystalline cellulose with a concomitant decrease in lignin in the cell walls of tension-stressed tissues. To investigate the molecular basis of this tree stress response, we cloned a full-length cellulose synthase (PtCesA) cDNA from developing xylem of aspen (Populus tremuloides). About 90% sequence similarity was found between the predicted PtCesA and cotton GhCesA proteins. Northern blot and in situ hybridization analyses of PtCesA gene transcripts in various aspen tissues, and PtCesA gene promoter-beta-glucuronidase (GUS) fusion analysis in transgenic tobacco, demonstrated conclusively that PtCesA expression is confined to developing xylem cells during normal plant growth. During mechanical stress induced by stem bending, GUS expression remained in xylem and was induced in developing phloem fibers undergoing tension stress, but was turned off in tissues undergoing compression on the opposite side of the bend. Our results suggest a unique role for PtCesA in cellulose biosynthesis in both tension-stressed and normal tissues in aspen, and that the on/off control of PtCesA expression may be a part of a signaling mechanism triggering a stress-related compensatory deposition of cellulose and lignin that is crucial to growth and development in trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton 49931, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joshi</LastName><ForeName>C P</ForeName><Initials>CP</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>V L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C457769">PRC1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.12</RegistryNumber><NameOfSubstance UI="C028634">cellulose synthase 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Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010947" MajorTopicYN="N">Plants, Toxic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013314" MajorTopicYN="N">Stress, Mechanical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>7</Month><Day>8</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>9</Month><Day>23</Day><Hour>11</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>7</Month><Day>8</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10886769</ArticleId><ArticleId IdType="pii">tpj758</ArticleId><ArticleId IdType="doi">10.1046/j.1365-313x.2000.00758.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Chiang, V L" sort="Chiang, V L" uniqKey="Chiang V" first="V L" last="Chiang">V L Chiang</name><name sortKey="Joshi, C P" sort="Joshi, C P" uniqKey="Joshi C" first="C P" last="Joshi">C P Joshi</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Wu, L" sort="Wu, L" uniqKey="Wu L" first="L" last="Wu">L. 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