The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.
Identifieur interne : 004652 ( Main/Exploration ); précédent : 004651; suivant : 004653The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.
Auteurs : M. Milani [Italie] ; L. Andolfi ; S. Cannistraro ; M P Verbeet ; M. BolognesiSource :
- Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2001.
Descripteurs français
- KwdFr :
- Arbres (composition chimique), Conformation des protéines (MeSH), Cristallisation (MeSH), Cristallographie aux rayons X (MeSH), Ingénierie des protéines (MeSH), Modèles moléculaires (MeSH), Mutation (MeSH), Plastocyanine (composition chimique), Plastocyanine (génétique), Protéines recombinantes (composition chimique).
- MESH :
- composition chimique : Arbres, Plastocyanine, Protéines recombinantes.
- génétique : Plastocyanine.
- Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Ingénierie des protéines, Modèles moléculaires, Mutation.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Plastocyanin, Recombinant Proteins.
- chemical , genetics : Plastocyanin.
- chemistry : Trees.
- Crystallization, Crystallography, X-Ray, Models, Molecular, Mutation, Protein Conformation, Protein Engineering.
Abstract
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
DOI: 10.1107/s0907444901013221
PubMed: 11679761
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Cannistraro</name></author><author><name sortKey="Verbeet, M P" sort="Verbeet, M P" uniqKey="Verbeet M" first="M P" last="Verbeet">M P Verbeet</name></author><author><name sortKey="Bolognesi, M" sort="Bolognesi, M" uniqKey="Bolognesi M" first="M" last="Bolognesi">M. Bolognesi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11679761</idno><idno type="pmid">11679761</idno><idno type="doi">10.1107/s0907444901013221</idno><idno type="wicri:Area/Main/Corpus">004720</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004720</idno><idno type="wicri:Area/Main/Curation">004720</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004720</idno><idno type="wicri:Area/Main/Exploration">004720</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.</title><author><name sortKey="Milani, M" sort="Milani, M" uniqKey="Milani M" first="M" last="Milani">M. Milani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physics-INFM, c/o Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi 10, 16132 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Physics-INFM, c/o Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi 10, 16132 Genova</wicri:regionArea><wicri:noRegion>16132 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Andolfi, L" sort="Andolfi, L" uniqKey="Andolfi L" first="L" last="Andolfi">L. Andolfi</name></author><author><name sortKey="Cannistraro, S" sort="Cannistraro, S" uniqKey="Cannistraro S" first="S" last="Cannistraro">S. Cannistraro</name></author><author><name sortKey="Verbeet, M P" sort="Verbeet, M P" uniqKey="Verbeet M" first="M P" last="Verbeet">M P Verbeet</name></author><author><name sortKey="Bolognesi, M" sort="Bolognesi, M" uniqKey="Bolognesi M" first="M" last="Bolognesi">M. Bolognesi</name></author></analytic><series><title level="j">Acta crystallographica. Section D, Biological crystallography</title><idno type="ISSN">0907-4449</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization (MeSH)</term><term>Crystallography, X-Ray (MeSH)</term><term>Models, Molecular (MeSH)</term><term>Mutation (MeSH)</term><term>Plastocyanin (chemistry)</term><term>Plastocyanin (genetics)</term><term>Protein Conformation (MeSH)</term><term>Protein Engineering (MeSH)</term><term>Recombinant Proteins (chemistry)</term><term>Trees (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (composition chimique)</term><term>Conformation des protéines (MeSH)</term><term>Cristallisation (MeSH)</term><term>Cristallographie aux rayons X (MeSH)</term><term>Ingénierie des protéines (MeSH)</term><term>Modèles moléculaires (MeSH)</term><term>Mutation (MeSH)</term><term>Plastocyanine (composition chimique)</term><term>Plastocyanine (génétique)</term><term>Protéines recombinantes (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plastocyanin</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plastocyanin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Arbres</term><term>Plastocyanine</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Plastocyanine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Models, Molecular</term><term>Mutation</term><term>Protein Conformation</term><term>Protein Engineering</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Ingénierie des protéines</term><term>Modèles moléculaires</term><term>Mutation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11679761</PMID><DateCompleted><Year>2001</Year><Month>12</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0907-4449</ISSN><JournalIssue CitedMedium="Print"><Volume>57</Volume><Issue>Pt 11</Issue><PubDate><Year>2001</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Acta crystallographica. Section D, Biological crystallography</Title><ISOAbbreviation>Acta Crystallogr D Biol Crystallogr</ISOAbbreviation></Journal><ArticleTitle>The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.</ArticleTitle><Pagination><MedlinePgn>1735-8</MedlinePgn></Pagination><Abstract><AbstractText>Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Milani</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Physics-INFM, c/o Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi 10, 16132 Genova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andolfi</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Cannistraro</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Verbeet</LastName><ForeName>M P</ForeName><Initials>MP</Initials></Author><Author ValidYN="Y"><LastName>Bolognesi</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>PDB</DataBankName><AccessionNumberList><AccessionNumber>1JXG</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2001</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Acta Crystallogr D Biol Crystallogr</MedlineTA><NlmUniqueID>9305878</NlmUniqueID><ISSNLinking>0907-4449</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9014-09-9</RegistryNumber><NameOfSubstance UI="D010970">Plastocyanin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010970" MajorTopicYN="N">Plastocyanin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2001</Year><Month>07</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2001</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>10</Month><Day>27</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>10</Month><Day>27</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11679761</ArticleId><ArticleId IdType="pii">S0907444901013221</ArticleId><ArticleId IdType="doi">10.1107/s0907444901013221</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Andolfi, L" sort="Andolfi, L" uniqKey="Andolfi L" first="L" last="Andolfi">L. Andolfi</name><name sortKey="Bolognesi, M" sort="Bolognesi, M" uniqKey="Bolognesi M" first="M" last="Bolognesi">M. Bolognesi</name><name sortKey="Cannistraro, S" sort="Cannistraro, S" uniqKey="Cannistraro S" first="S" last="Cannistraro">S. Cannistraro</name><name sortKey="Verbeet, M P" sort="Verbeet, M P" uniqKey="Verbeet M" first="M P" last="Verbeet">M P Verbeet</name></noCountry><country name="Italie"><noRegion><name sortKey="Milani, M" sort="Milani, M" uniqKey="Milani M" first="M" last="Milani">M. Milani</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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