Genetics of resistance to transgenic Bacillus thuringiensis poplars in Chrysomela tremulae (Coleoptera: Chrysomelidae).
Identifieur interne : 004255 ( Main/Exploration ); précédent : 004254; suivant : 004256Genetics of resistance to transgenic Bacillus thuringiensis poplars in Chrysomela tremulae (Coleoptera: Chrysomelidae).
Auteurs : Sylvie Augustin [France] ; Claudine Courtin ; Agnes Rejasse ; Philippe Lorme ; Anne Genissel ; Denis BourguetSource :
- Journal of economic entomology [ 0022-0493 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Coléoptères (croissance et développement), Coléoptères (génétique), Endotoxines (génétique), Feuilles de plante (MeSH), Hémolysines (MeSH), Populus (génétique), Protéines bactériennes (génétique), Résistance aux insecticides (génétique), Toxines bactériennes (génétique), Végétaux génétiquement modifiés (génétique).
- MESH :
- croissance et développement : Coléoptères.
- génétique : Coléoptères, Endotoxines, Populus, Protéines bactériennes, Résistance aux insecticides, Toxines bactériennes, Végétaux génétiquement modifiés.
- Animaux, Feuilles de plante, Hémolysines.
English descriptors
- KwdEn :
- Animals (MeSH), Bacterial Proteins (genetics), Bacterial Toxins (genetics), Coleoptera (genetics), Coleoptera (growth & development), Endotoxins (genetics), Hemolysin Proteins (MeSH), Insecticide Resistance (genetics), Plant Leaves (MeSH), Plants, Genetically Modified (genetics), Populus (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, Bacterial Toxins, Endotoxins.
- genetics : Coleoptera, Insecticide Resistance, Plants, Genetically Modified, Populus.
- growth & development : Coleoptera.
- Animals, Hemolysin Proteins, Plant Leaves.
Abstract
The area under genetically engineered plants producing Bacillus thuringiensis (Bt) toxins is steadily increasing. This increase has magnified the risk of alleles conferring resistance to these toxins being selected in natural populations of target insect pests. The speed at which this selection is likely to occur depends on the genetic characteristics of Bt resistance. We selected a strain of the beetle Chrysomela tremulae Fabricius on a transgenic Bt poplar clone Populus tremula L. x Populus tremuloides Michx producing high levels of B. thuringiensis Cry3Aa toxin. This strain was derived from an isofemale line that generated some F2 offspring that actively fed on this Bt poplar clone. The resistance ratio of the strain was >6400. Susceptibility had decreased to such an extent that the mortality of beetles of the strain fed Bt poplar leaves was similar to that of beetles fed nontransgenic poplar leaves. Genetic crosses between susceptible, resistant, and F1 hybrids showed that resistance to the Cry3Aa toxin was almost completely recessive (D(LC) = 0.07) and conferred by a single autosomal gene. The concentration of Cry3Aa produced in the transgenic Bt poplar used in this study was 6.34 times higher than the LC99 of the F1 hybrids, accounting for the complete recessivity (D(ML) = 0) of survival on Bt poplar leaves. Overall, the genetic characteristics of the resistance of C. tremulae to the Cry3Aa toxin are consistent with the assumptions underlying the high-dose refuge strategy, which aims to decrease the selection of Bt resistance alleles in natural target pest populations.
DOI: 10.1093/jee/97.3.1058
PubMed: 15279291
Affiliations:
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Courtin</name></author><author><name sortKey="Rejasse, Agnes" sort="Rejasse, Agnes" uniqKey="Rejasse A" first="Agnes" last="Rejasse">Agnes Rejasse</name></author><author><name sortKey="Lorme, Philippe" sort="Lorme, Philippe" uniqKey="Lorme P" first="Philippe" last="Lorme">Philippe Lorme</name></author><author><name sortKey="Genissel, Anne" sort="Genissel, Anne" uniqKey="Genissel A" first="Anne" last="Genissel">Anne Genissel</name></author><author><name sortKey="Bourguet, Denis" sort="Bourguet, Denis" uniqKey="Bourguet D" first="Denis" last="Bourguet">Denis Bourguet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15279291</idno><idno type="pmid">15279291</idno><idno type="doi">10.1093/jee/97.3.1058</idno><idno type="wicri:Area/Main/Corpus">004233</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004233</idno><idno 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Olivet</wicri:noRegion><wicri:noRegion>45 166 Olivet</wicri:noRegion></affiliation></author><author><name sortKey="Courtin, Claudine" sort="Courtin, Claudine" uniqKey="Courtin C" first="Claudine" last="Courtin">Claudine Courtin</name></author><author><name sortKey="Rejasse, Agnes" sort="Rejasse, Agnes" uniqKey="Rejasse A" first="Agnes" last="Rejasse">Agnes Rejasse</name></author><author><name sortKey="Lorme, Philippe" sort="Lorme, Philippe" uniqKey="Lorme P" first="Philippe" last="Lorme">Philippe Lorme</name></author><author><name sortKey="Genissel, Anne" sort="Genissel, Anne" uniqKey="Genissel A" first="Anne" last="Genissel">Anne Genissel</name></author><author><name sortKey="Bourguet, Denis" sort="Bourguet, Denis" uniqKey="Bourguet D" first="Denis" last="Bourguet">Denis Bourguet</name></author></analytic><series><title level="j">Journal of economic entomology</title><idno type="ISSN">0022-0493</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Toxins (genetics)</term><term>Coleoptera (genetics)</term><term>Coleoptera (growth & development)</term><term>Endotoxins (genetics)</term><term>Hemolysin Proteins (MeSH)</term><term>Insecticide Resistance (genetics)</term><term>Plant Leaves (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Coléoptères (croissance et développement)</term><term>Coléoptères (génétique)</term><term>Endotoxines (génétique)</term><term>Feuilles de plante (MeSH)</term><term>Hémolysines (MeSH)</term><term>Populus (génétique)</term><term>Protéines bactériennes (génétique)</term><term>Résistance aux insecticides (génétique)</term><term>Toxines bactériennes (génétique)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Bacterial Toxins</term><term>Endotoxins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coléoptères</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coleoptera</term><term>Insecticide Resistance</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Coleoptera</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coléoptères</term><term>Endotoxines</term><term>Populus</term><term>Protéines bactériennes</term><term>Résistance aux insecticides</term><term>Toxines bactériennes</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Hemolysin Proteins</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Feuilles de plante</term><term>Hémolysines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The area under genetically engineered plants producing Bacillus thuringiensis (Bt) toxins is steadily increasing. This increase has magnified the risk of alleles conferring resistance to these toxins being selected in natural populations of target insect pests. The speed at which this selection is likely to occur depends on the genetic characteristics of Bt resistance. We selected a strain of the beetle Chrysomela tremulae Fabricius on a transgenic Bt poplar clone Populus tremula L. x Populus tremuloides Michx producing high levels of B. thuringiensis Cry3Aa toxin. This strain was derived from an isofemale line that generated some F2 offspring that actively fed on this Bt poplar clone. The resistance ratio of the strain was >6400. Susceptibility had decreased to such an extent that the mortality of beetles of the strain fed Bt poplar leaves was similar to that of beetles fed nontransgenic poplar leaves. Genetic crosses between susceptible, resistant, and F1 hybrids showed that resistance to the Cry3Aa toxin was almost completely recessive (D(LC) = 0.07) and conferred by a single autosomal gene. The concentration of Cry3Aa produced in the transgenic Bt poplar used in this study was 6.34 times higher than the LC99 of the F1 hybrids, accounting for the complete recessivity (D(ML) = 0) of survival on Bt poplar leaves. Overall, the genetic characteristics of the resistance of C. tremulae to the Cry3Aa toxin are consistent with the assumptions underlying the high-dose refuge strategy, which aims to decrease the selection of Bt resistance alleles in natural target pest populations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15279291</PMID><DateCompleted><Year>2004</Year><Month>09</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-0493</ISSN><JournalIssue CitedMedium="Print"><Volume>97</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of economic entomology</Title><ISOAbbreviation>J Econ Entomol</ISOAbbreviation></Journal><ArticleTitle>Genetics of resistance to transgenic Bacillus thuringiensis poplars in Chrysomela tremulae (Coleoptera: Chrysomelidae).</ArticleTitle><Pagination><MedlinePgn>1058-64</MedlinePgn></Pagination><Abstract><AbstractText>The area under genetically engineered plants producing Bacillus thuringiensis (Bt) toxins is steadily increasing. This increase has magnified the risk of alleles conferring resistance to these toxins being selected in natural populations of target insect pests. The speed at which this selection is likely to occur depends on the genetic characteristics of Bt resistance. We selected a strain of the beetle Chrysomela tremulae Fabricius on a transgenic Bt poplar clone Populus tremula L. x Populus tremuloides Michx producing high levels of B. thuringiensis Cry3Aa toxin. This strain was derived from an isofemale line that generated some F2 offspring that actively fed on this Bt poplar clone. The resistance ratio of the strain was >6400. Susceptibility had decreased to such an extent that the mortality of beetles of the strain fed Bt poplar leaves was similar to that of beetles fed nontransgenic poplar leaves. Genetic crosses between susceptible, resistant, and F1 hybrids showed that resistance to the Cry3Aa toxin was almost completely recessive (D(LC) = 0.07) and conferred by a single autosomal gene. The concentration of Cry3Aa produced in the transgenic Bt poplar used in this study was 6.34 times higher than the LC99 of the F1 hybrids, accounting for the complete recessivity (D(ML) = 0) of survival on Bt poplar leaves. Overall, the genetic characteristics of the resistance of C. tremulae to the Cry3Aa toxin are consistent with the assumptions underlying the high-dose refuge strategy, which aims to decrease the selection of Bt resistance alleles in natural target pest populations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Augustin</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique, Centre de Recherches d'Orléans, Unité de Zoologie Forestière, BP 20619 Ardon, 45 166 Olivet, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Courtin</LastName><ForeName>Claudine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Rejasse</LastName><ForeName>Agnes</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Lorme</LastName><ForeName>Philippe</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Genissel</LastName><ForeName>Anne</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bourguet</LastName><ForeName>Denis</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Econ Entomol</MedlineTA><NlmUniqueID>2985127R</NlmUniqueID><ISSNLinking>0022-0493</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001427">Bacterial Toxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C030956">insecticidal crystal protein, Bacillus Thuringiensis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001427" MajorTopicYN="N">Bacterial Toxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001517" MajorTopicYN="N">Coleoptera</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004731" MajorTopicYN="N">Endotoxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007305" MajorTopicYN="N">Insecticide Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>7</Month><Day>29</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>9</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>7</Month><Day>29</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15279291</ArticleId><ArticleId IdType="doi">10.1093/jee/97.3.1058</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Bourguet, Denis" sort="Bourguet, Denis" uniqKey="Bourguet D" first="Denis" last="Bourguet">Denis Bourguet</name><name sortKey="Courtin, Claudine" sort="Courtin, Claudine" uniqKey="Courtin C" first="Claudine" last="Courtin">Claudine Courtin</name><name sortKey="Genissel, Anne" sort="Genissel, Anne" uniqKey="Genissel A" first="Anne" last="Genissel">Anne Genissel</name><name sortKey="Lorme, Philippe" sort="Lorme, Philippe" uniqKey="Lorme P" first="Philippe" last="Lorme">Philippe Lorme</name><name sortKey="Rejasse, Agnes" sort="Rejasse, Agnes" uniqKey="Rejasse A" first="Agnes" last="Rejasse">Agnes Rejasse</name></noCountry><country name="France"><noRegion><name sortKey="Augustin, Sylvie" sort="Augustin, Sylvie" uniqKey="Augustin S" first="Sylvie" last="Augustin">Sylvie Augustin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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