Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF.
Identifieur interne : 004146 ( Main/Exploration ); précédent : 004145; suivant : 004147Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF.
Auteurs : Maria Cantarella [Italie] ; Laura Cantarella ; Alberto Gallifuoco ; Agata Spera ; Francesco AlfaniSource :
- Biotechnology progress [ 8756-7938 ]
Descripteurs français
- KwdFr :
- Antienzymes (composition chimique), Antienzymes (métabolisme), Bioréacteurs (microbiologie), Bois (MeSH), Extraits de plantes (composition chimique), Extraits de plantes (métabolisme), Glucose (métabolisme), Glucosidases (métabolisme), Hydrolases (métabolisme), Hydrolyse (MeSH), Populus (composition chimique), Populus (microbiologie), Saccharomyces cerevisiae (métabolisme), Vapeur (MeSH), Éthanol (métabolisme).
- MESH :
- composition chimique : Antienzymes, Extraits de plantes, Populus.
- microbiologie : Bioréacteurs, Populus.
- métabolisme : Antienzymes, Extraits de plantes, Glucose, Glucosidases, Hydrolases, Saccharomyces cerevisiae, Éthanol.
- Bois, Hydrolyse, Vapeur.
English descriptors
- KwdEn :
- Bioreactors (microbiology), Enzyme Inhibitors (chemistry), Enzyme Inhibitors (metabolism), Ethanol (metabolism), Glucose (metabolism), Glucosidases (metabolism), Hydrolases (metabolism), Hydrolysis (MeSH), Plant Extracts (chemistry), Plant Extracts (metabolism), Populus (chemistry), Populus (microbiology), Saccharomyces cerevisiae (metabolism), Steam (MeSH), Wood (MeSH).
- MESH :
- chemical , chemistry : Enzyme Inhibitors, Plant Extracts.
- chemical , metabolism : Enzyme Inhibitors, Ethanol, Glucose, Glucosidases, Hydrolases, Plant Extracts.
- chemistry : Populus.
- metabolism : Saccharomyces cerevisiae.
- microbiology : Bioreactors, Populus.
- Hydrolysis, Steam, Wood.
Abstract
Steam-exploded (SE) poplar wood biomass was hydrolyzed by means of a blend of Celluclast and Novozym cellulase complexes in the presence of the inhibiting compounds produced during the preceding steam-explosion pretreatment process. The SE temperature and time conditions were 214 degrees C and 6 min, resulting in a log R(0) of 4.13. In enzymatic hydrolysis tests at 45 degrees C, the biomass loading in the bioreactor was 100 g(DW)/L (dry weight) and the enzyme-to-biomass ratio 0.06 g/g(DW). The enzyme activities for endo-glucanase, exo-glucanase, and beta-glucosidase were 5.76, 0.55, and 5.98 U/mg, respectively. The inhibiting effects of components released during SE (formic, acetic, and levulinic acids, furfural, 5-hydroxymethyl furfural (5-HMF), syringaldehyde, 4-hydroxy benzaldehyde, and vanillin) were studied at different concentrations in hydrolysis runs performed with rinsed SE biomass as model substrate. Acetic acid (2 g/L), furfural, 5-HMF, syringaldehyde, 4-hydroxybenzaldehyde, and vanillin (0.5 g/L) did not significantly effect the enzyme activity, whereas formic acid (11.5 g/L) inactivated the enzymes and levulinic acid (29.0 g/L) partially affected the cellulase. Synergism and cumulative concentration effects of these compounds were not detected. SSF experiments show that untreated SE biomass during the enzymatic attack gives rise to a nonfermentable hydrolysate, which becomes fermentable when rinsed SE biomass is used. The presence of acetic acid, vanillin, and 5-HMF (0.5 g/L) in SSF of 100 g(DW) /L biomass gave rise to ethanol yields of 84.0%, 73.5%, and 91.0% respectively, with respective lag phases of 42, 39, and 58 h.
DOI: 10.1021/bp0257978
PubMed: 14763843
Affiliations:
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Le document en format XML
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sortKey="Gallifuoco, Alberto" sort="Gallifuoco, Alberto" uniqKey="Gallifuoco A" first="Alberto" last="Gallifuoco">Alberto Gallifuoco</name></author><author><name sortKey="Spera, Agata" sort="Spera, Agata" uniqKey="Spera A" first="Agata" last="Spera">Agata Spera</name></author><author><name sortKey="Alfani, Francesco" sort="Alfani, Francesco" uniqKey="Alfani F" first="Francesco" last="Alfani">Francesco Alfani</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004 Jan-Feb</date><idno type="RBID">pubmed:14763843</idno><idno type="pmid">14763843</idno><idno type="doi">10.1021/bp0257978</idno><idno type="wicri:Area/Main/Corpus">004342</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004342</idno><idno type="wicri:Area/Main/Curation">004342</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004342</idno><idno 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Cantarella</name></author><author><name sortKey="Gallifuoco, Alberto" sort="Gallifuoco, Alberto" uniqKey="Gallifuoco A" first="Alberto" last="Gallifuoco">Alberto Gallifuoco</name></author><author><name sortKey="Spera, Agata" sort="Spera, Agata" uniqKey="Spera A" first="Agata" last="Spera">Agata Spera</name></author><author><name sortKey="Alfani, Francesco" sort="Alfani, Francesco" uniqKey="Alfani F" first="Francesco" last="Alfani">Francesco Alfani</name></author></analytic><series><title level="j">Biotechnology progress</title><idno type="ISSN">8756-7938</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bioreactors (microbiology)</term><term>Enzyme Inhibitors (chemistry)</term><term>Enzyme Inhibitors (metabolism)</term><term>Ethanol (metabolism)</term><term>Glucose (metabolism)</term><term>Glucosidases (metabolism)</term><term>Hydrolases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (metabolism)</term><term>Populus (chemistry)</term><term>Populus (microbiology)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Steam (MeSH)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antienzymes (composition chimique)</term><term>Antienzymes (métabolisme)</term><term>Bioréacteurs (microbiologie)</term><term>Bois (MeSH)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (métabolisme)</term><term>Glucose (métabolisme)</term><term>Glucosidases (métabolisme)</term><term>Hydrolases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Populus (composition chimique)</term><term>Populus (microbiologie)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Vapeur (MeSH)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Enzyme Inhibitors</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Enzyme Inhibitors</term><term>Ethanol</term><term>Glucose</term><term>Glucosidases</term><term>Hydrolases</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antienzymes</term><term>Extraits de plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bioréacteurs</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bioreactors</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antienzymes</term><term>Extraits de plantes</term><term>Glucose</term><term>Glucosidases</term><term>Hydrolases</term><term>Saccharomyces cerevisiae</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrolysis</term><term>Steam</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bois</term><term>Hydrolyse</term><term>Vapeur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Steam-exploded (SE) poplar wood biomass was hydrolyzed by means of a blend of Celluclast and Novozym cellulase complexes in the presence of the inhibiting compounds produced during the preceding steam-explosion pretreatment process. The SE temperature and time conditions were 214 degrees C and 6 min, resulting in a log R(0) of 4.13. In enzymatic hydrolysis tests at 45 degrees C, the biomass loading in the bioreactor was 100 g(DW)/L (dry weight) and the enzyme-to-biomass ratio 0.06 g/g(DW). The enzyme activities for endo-glucanase, exo-glucanase, and beta-glucosidase were 5.76, 0.55, and 5.98 U/mg, respectively. The inhibiting effects of components released during SE (formic, acetic, and levulinic acids, furfural, 5-hydroxymethyl furfural (5-HMF), syringaldehyde, 4-hydroxy benzaldehyde, and vanillin) were studied at different concentrations in hydrolysis runs performed with rinsed SE biomass as model substrate. Acetic acid (2 g/L), furfural, 5-HMF, syringaldehyde, 4-hydroxybenzaldehyde, and vanillin (0.5 g/L) did not significantly effect the enzyme activity, whereas formic acid (11.5 g/L) inactivated the enzymes and levulinic acid (29.0 g/L) partially affected the cellulase. Synergism and cumulative concentration effects of these compounds were not detected. SSF experiments show that untreated SE biomass during the enzymatic attack gives rise to a nonfermentable hydrolysate, which becomes fermentable when rinsed SE biomass is used. The presence of acetic acid, vanillin, and 5-HMF (0.5 g/L) in SSF of 100 g(DW) /L biomass gave rise to ethanol yields of 84.0%, 73.5%, and 91.0% respectively, with respective lag phases of 42, 39, and 58 h.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14763843</PMID><DateCompleted><Year>2004</Year><Month>10</Month><Day>08</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">8756-7938</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>1</Issue><PubDate><MedlineDate>2004 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF.</ArticleTitle><Pagination><MedlinePgn>200-6</MedlinePgn></Pagination><Abstract><AbstractText>Steam-exploded (SE) poplar wood biomass was hydrolyzed by means of a blend of Celluclast and Novozym cellulase complexes in the presence of the inhibiting compounds produced during the preceding steam-explosion pretreatment process. The SE temperature and time conditions were 214 degrees C and 6 min, resulting in a log R(0) of 4.13. In enzymatic hydrolysis tests at 45 degrees C, the biomass loading in the bioreactor was 100 g(DW)/L (dry weight) and the enzyme-to-biomass ratio 0.06 g/g(DW). The enzyme activities for endo-glucanase, exo-glucanase, and beta-glucosidase were 5.76, 0.55, and 5.98 U/mg, respectively. The inhibiting effects of components released during SE (formic, acetic, and levulinic acids, furfural, 5-hydroxymethyl furfural (5-HMF), syringaldehyde, 4-hydroxy benzaldehyde, and vanillin) were studied at different concentrations in hydrolysis runs performed with rinsed SE biomass as model substrate. Acetic acid (2 g/L), furfural, 5-HMF, syringaldehyde, 4-hydroxybenzaldehyde, and vanillin (0.5 g/L) did not significantly effect the enzyme activity, whereas formic acid (11.5 g/L) inactivated the enzymes and levulinic acid (29.0 g/L) partially affected the cellulase. Synergism and cumulative concentration effects of these compounds were not detected. SSF experiments show that untreated SE biomass during the enzymatic attack gives rise to a nonfermentable hydrolysate, which becomes fermentable when rinsed SE biomass is used. The presence of acetic acid, vanillin, and 5-HMF (0.5 g/L) in SSF of 100 g(DW) /L biomass gave rise to ethanol yields of 84.0%, 73.5%, and 91.0% respectively, with respective lag phases of 42, 39, and 58 h.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cantarella</LastName><ForeName>Maria</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, Monteluco di Roio, 67040 L'Aquila, Italy. cantarel@ing.univaq.it</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cantarella</LastName><ForeName>Laura</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Gallifuoco</LastName><ForeName>Alberto</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Spera</LastName><ForeName>Agata</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Alfani</LastName><ForeName>Francesco</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013227">Steam</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.-</RegistryNumber><NameOfSubstance UI="D006867">Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D005959">Glucosidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019149" MajorTopicYN="N">Bioreactors</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005959" MajorTopicYN="N">Glucosidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006867" MajorTopicYN="N">Hydrolases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013227" MajorTopicYN="Y">Steam</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="Y">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>2</Month><Day>7</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>10</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>2</Month><Day>7</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14763843</ArticleId><ArticleId IdType="doi">10.1021/bp0257978</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Alfani, Francesco" sort="Alfani, Francesco" uniqKey="Alfani F" first="Francesco" last="Alfani">Francesco Alfani</name><name sortKey="Cantarella, Laura" sort="Cantarella, Laura" uniqKey="Cantarella L" first="Laura" last="Cantarella">Laura Cantarella</name><name sortKey="Gallifuoco, Alberto" sort="Gallifuoco, Alberto" uniqKey="Gallifuoco A" first="Alberto" last="Gallifuoco">Alberto Gallifuoco</name><name sortKey="Spera, Agata" sort="Spera, Agata" uniqKey="Spera A" first="Agata" last="Spera">Agata Spera</name></noCountry><country name="Italie"><noRegion><name sortKey="Cantarella, 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