Immunolocalization of phenylalanine ammonia-lyase and cinnamate-4-hydroxylase in differentiating xylem of poplar.
Identifieur interne : 004128 ( Main/Exploration ); précédent : 004127; suivant : 004129Immunolocalization of phenylalanine ammonia-lyase and cinnamate-4-hydroxylase in differentiating xylem of poplar.
Auteurs : Takahiko Sato [Japon] ; Keiji Takabe ; Minoru FujitaSource :
- Comptes rendus biologies [ 1631-0691 ]
Descripteurs français
- KwdFr :
- MESH :
- analyse : Cytochrome P-450 enzyme system, Mixed function oxygenases, Phenylalanine ammonia-lyase.
- composition chimique : Populus, Structures de plante.
- Dosage immunologique, Trans-cinnamate 4-monooxygenase.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Cytochrome P-450 Enzyme System, Mixed Function Oxygenases, Phenylalanine Ammonia-Lyase.
- chemistry : Plant Structures, Populus.
- Immunoassay, Trans-Cinnamate 4-Monooxygenase.
Abstract
Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and cinnamate-4-hydroxylase (C4H; EC 1.14.13.11) are pivotal enzymes involved in lignification. We synthesized peptides as the epitopes according to the amino acid sequences of these enzymes, coupled them with hemocyanin, and injected them into mice. The antiserums against peptides of PAL and C4H specifically detected PAL and C4H in the crude enzymes extracted from differentiating xylem of poplar, respectively. PAL and C4H were localized in differentiating xylem of poplar. PAL labeling was mainly localized in the cytosol, and somewhat localized on the rough-endoplasmic reticulum (r-ER) and the Golgi apparatus. In contrast, C4H was mainly observed on r-ER and the Golgi apparatus. These findings suggest that conversion of phenylalanine to cinnamic acid occurs in the cytosol and the following reaction occurs near the membrane of r-ER and the Golgi apparatus. The possibility of coordinated localization of PAL and C4H is discussed.
DOI: 10.1016/j.crvi.2004.08.005
PubMed: 15587074
Affiliations:
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Le document en format XML
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We synthesized peptides as the epitopes according to the amino acid sequences of these enzymes, coupled them with hemocyanin, and injected them into mice. The antiserums against peptides of PAL and C4H specifically detected PAL and C4H in the crude enzymes extracted from differentiating xylem of poplar, respectively. PAL and C4H were localized in differentiating xylem of poplar. PAL labeling was mainly localized in the cytosol, and somewhat localized on the rough-endoplasmic reticulum (r-ER) and the Golgi apparatus. In contrast, C4H was mainly observed on r-ER and the Golgi apparatus. These findings suggest that conversion of phenylalanine to cinnamic acid occurs in the cytosol and the following reaction occurs near the membrane of r-ER and the Golgi apparatus. The possibility of coordinated localization of PAL and C4H is discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15587074</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1631-0691</ISSN><JournalIssue CitedMedium="Print"><Volume>327</Volume><Issue>9-10</Issue><PubDate><MedlineDate>2004 Sep-Oct</MedlineDate></PubDate></JournalIssue><Title>Comptes rendus biologies</Title><ISOAbbreviation>C R Biol</ISOAbbreviation></Journal><ArticleTitle>Immunolocalization of phenylalanine ammonia-lyase and cinnamate-4-hydroxylase in differentiating xylem of poplar.</ArticleTitle><Pagination><MedlinePgn>827-36</MedlinePgn></Pagination><Abstract><AbstractText>Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and cinnamate-4-hydroxylase (C4H; EC 1.14.13.11) are pivotal enzymes involved in lignification. We synthesized peptides as the epitopes according to the amino acid sequences of these enzymes, coupled them with hemocyanin, and injected them into mice. The antiserums against peptides of PAL and C4H specifically detected PAL and C4H in the crude enzymes extracted from differentiating xylem of poplar, respectively. PAL and C4H were localized in differentiating xylem of poplar. PAL labeling was mainly localized in the cytosol, and somewhat localized on the rough-endoplasmic reticulum (r-ER) and the Golgi apparatus. In contrast, C4H was mainly observed on r-ER and the Golgi apparatus. These findings suggest that conversion of phenylalanine to cinnamic acid occurs in the cytosol and the following reaction occurs near the membrane of r-ER and the Golgi apparatus. The possibility of coordinated localization of PAL and C4H is discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sato</LastName><ForeName>Takahiko</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Tree Cell Biology, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Takabe</LastName><ForeName>Keiji</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Fujita</LastName><ForeName>Minoru</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>C R Biol</MedlineTA><NlmUniqueID>101140040</NlmUniqueID><ISSNLinking>1631-0691</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9035-51-2</RegistryNumber><NameOfSubstance UI="D003577">Cytochrome P-450 Enzyme System</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.14.91</RegistryNumber><NameOfSubstance UI="D050564">Trans-Cinnamate 4-Monooxygenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.3.1.24</RegistryNumber><NameOfSubstance UI="D010650">Phenylalanine Ammonia-Lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003577" MajorTopicYN="N">Cytochrome P-450 Enzyme System</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007118" MajorTopicYN="N">Immunoassay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010650" MajorTopicYN="N">Phenylalanine Ammonia-Lyase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018514" MajorTopicYN="N">Plant Structures</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050564" MajorTopicYN="N">Trans-Cinnamate 4-Monooxygenase</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15587074</ArticleId><ArticleId IdType="pii">S1631-0691(04)00182-9</ArticleId><ArticleId IdType="doi">10.1016/j.crvi.2004.08.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Fujita, Minoru" sort="Fujita, Minoru" uniqKey="Fujita M" first="Minoru" last="Fujita">Minoru Fujita</name><name sortKey="Takabe, Keiji" sort="Takabe, Keiji" uniqKey="Takabe K" first="Keiji" last="Takabe">Keiji Takabe</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Sato, Takahiko" sort="Sato, Takahiko" uniqKey="Sato T" first="Takahiko" last="Sato">Takahiko Sato</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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