Lignin isolated from primary walls of hybrid aspen cell cultures indicates significant differences in lignin structure between primary and secondary cell wall.
Identifieur interne : 004007 ( Main/Exploration ); précédent : 004006; suivant : 004008Lignin isolated from primary walls of hybrid aspen cell cultures indicates significant differences in lignin structure between primary and secondary cell wall.
Auteurs : Maria Christiernin [Suède] ; Anna B. Ohlsson ; Torkel Berglund ; Gunnar HenrikssonSource :
- Plant physiology and biochemistry : PPB [ 0981-9428 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Lignine, Paroi cellulaire, Populus.
- cytologie : Populus.
- isolement et purification : Lignine.
- Cellules cultivées, Facteurs temps.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Lignin.
- chemistry : Cell Wall, Populus.
- cytology : Populus.
- chemical , isolation & purification : Lignin.
- Cells, Cultured, Time Factors.
Abstract
Hybrid aspen (Populus tremula x tremuloides) cell cultures were grown for 7, 14 and 21 days. The cell cultures formed primary cell walls but no secondary cell wall according to carbohydrate analysis and microscopic characterization. The primary walls were lignified, increasingly with age, according to Klason lignin analysis. Presence of lignin in the primary walls, with a higher content in 21-day old cells than in 7-day old cells, was further supported by phloroglucinol/HCl reagent test and confocal microscopy after both immunolocalization and staining with acriflavin. Both laccase and peroxidase activity were found in the cultures and the activity increased during lignin formation. The lignin from the cell culture material was compared to lignin from mature aspen wood, where most of the lignin originates in the secondary cell wall, and which served as our secondary cell wall control. Lignin from the cell walls was isolated and characterized by thioacidolysis followed by gas chromatography and mass spectrometry. The lignin in the cell cultures differed from lignin of mature aspen wood in that it consisted exclusively of guaiacyl units, and had a more condensed structure. Five lignin structures were identified by mass spectrometry in the cell suspension cultures. The results indicate that the hybrid aspen cell culture used in this investigation may be a convenient experimental system for studies of primary cell wall lignin.
DOI: 10.1016/j.plaphy.2005.07.007
PubMed: 16198589
Affiliations:
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Ohlsson</name></author><author><name sortKey="Berglund, Torkel" sort="Berglund, Torkel" uniqKey="Berglund T" first="Torkel" last="Berglund">Torkel Berglund</name></author><author><name sortKey="Henriksson, Gunnar" sort="Henriksson, Gunnar" uniqKey="Henriksson G" first="Gunnar" last="Henriksson">Gunnar Henriksson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16198589</idno><idno type="pmid">16198589</idno><idno type="doi">10.1016/j.plaphy.2005.07.007</idno><idno type="wicri:Area/Main/Corpus">003F49</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003F49</idno><idno type="wicri:Area/Main/Curation">003F49</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003F49</idno><idno type="wicri:Area/Main/Exploration">003F49</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lignin isolated from primary walls of hybrid aspen cell cultures indicates significant differences in lignin structure between primary and secondary cell wall.</title><author><name sortKey="Christiernin, Maria" sort="Christiernin, Maria" uniqKey="Christiernin M" first="Maria" last="Christiernin">Maria Christiernin</name><affiliation wicri:level="1"><nlm:affiliation>Division of Wood Chemistry and Pulp Technology, Department of Fiber and Polymer Technology, School of Chemistry and Engineering, Royal Institute of Technology, KTH, 100 44 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Division of Wood Chemistry and Pulp Technology, Department of Fiber and Polymer Technology, School of Chemistry and Engineering, Royal Institute of Technology, KTH, 100 44 Stockholm</wicri:regionArea><wicri:noRegion>100 44 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Ohlsson, Anna B" sort="Ohlsson, Anna B" uniqKey="Ohlsson A" first="Anna B" last="Ohlsson">Anna B. Ohlsson</name></author><author><name sortKey="Berglund, Torkel" sort="Berglund, Torkel" uniqKey="Berglund T" first="Torkel" last="Berglund">Torkel Berglund</name></author><author><name sortKey="Henriksson, Gunnar" sort="Henriksson, Gunnar" uniqKey="Henriksson G" first="Gunnar" last="Henriksson">Gunnar Henriksson</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="ISSN">0981-9428</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Cells, Cultured (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (isolation & purification)</term><term>Populus (chemistry)</term><term>Populus (cytology)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (isolement et purification)</term><term>Paroi cellulaire (composition chimique)</term><term>Populus (composition chimique)</term><term>Populus (cytologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Facteurs temps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hybrid aspen (Populus tremula x tremuloides) cell cultures were grown for 7, 14 and 21 days. The cell cultures formed primary cell walls but no secondary cell wall according to carbohydrate analysis and microscopic characterization. The primary walls were lignified, increasingly with age, according to Klason lignin analysis. Presence of lignin in the primary walls, with a higher content in 21-day old cells than in 7-day old cells, was further supported by phloroglucinol/HCl reagent test and confocal microscopy after both immunolocalization and staining with acriflavin. Both laccase and peroxidase activity were found in the cultures and the activity increased during lignin formation. The lignin from the cell culture material was compared to lignin from mature aspen wood, where most of the lignin originates in the secondary cell wall, and which served as our secondary cell wall control. Lignin from the cell walls was isolated and characterized by thioacidolysis followed by gas chromatography and mass spectrometry. The lignin in the cell cultures differed from lignin of mature aspen wood in that it consisted exclusively of guaiacyl units, and had a more condensed structure. Five lignin structures were identified by mass spectrometry in the cell suspension cultures. The results indicate that the hybrid aspen cell culture used in this investigation may be a convenient experimental system for studies of primary cell wall lignin.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16198589</PMID><DateCompleted><Year>2006</Year><Month>05</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0981-9428</ISSN><JournalIssue CitedMedium="Print"><Volume>43</Volume><Issue>8</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Lignin isolated from primary walls of hybrid aspen cell cultures indicates significant differences in lignin structure between primary and secondary cell wall.</ArticleTitle><Pagination><MedlinePgn>777-85</MedlinePgn></Pagination><Abstract><AbstractText>Hybrid aspen (Populus tremula x tremuloides) cell cultures were grown for 7, 14 and 21 days. The cell cultures formed primary cell walls but no secondary cell wall according to carbohydrate analysis and microscopic characterization. The primary walls were lignified, increasingly with age, according to Klason lignin analysis. Presence of lignin in the primary walls, with a higher content in 21-day old cells than in 7-day old cells, was further supported by phloroglucinol/HCl reagent test and confocal microscopy after both immunolocalization and staining with acriflavin. Both laccase and peroxidase activity were found in the cultures and the activity increased during lignin formation. The lignin from the cell culture material was compared to lignin from mature aspen wood, where most of the lignin originates in the secondary cell wall, and which served as our secondary cell wall control. Lignin from the cell walls was isolated and characterized by thioacidolysis followed by gas chromatography and mass spectrometry. The lignin in the cell cultures differed from lignin of mature aspen wood in that it consisted exclusively of guaiacyl units, and had a more condensed structure. Five lignin structures were identified by mass spectrometry in the cell suspension cultures. The results indicate that the hybrid aspen cell culture used in this investigation may be a convenient experimental system for studies of primary cell wall lignin.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Christiernin</LastName><ForeName>Maria</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Division of Wood Chemistry and Pulp Technology, Department of Fiber and Polymer Technology, School of Chemistry and Engineering, Royal Institute of Technology, KTH, 100 44 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ohlsson</LastName><ForeName>Anna B</ForeName><Initials>AB</Initials></Author><Author ValidYN="Y"><LastName>Berglund</LastName><ForeName>Torkel</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Henriksson</LastName><ForeName>Gunnar</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>08</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2005</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>07</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>5</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16198589</ArticleId><ArticleId IdType="pii">S0981-9428(05)00176-2</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2005.07.007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Berglund, Torkel" sort="Berglund, Torkel" uniqKey="Berglund T" first="Torkel" last="Berglund">Torkel Berglund</name><name sortKey="Henriksson, Gunnar" sort="Henriksson, Gunnar" uniqKey="Henriksson G" first="Gunnar" last="Henriksson">Gunnar Henriksson</name><name sortKey="Ohlsson, Anna B" sort="Ohlsson, Anna B" uniqKey="Ohlsson A" first="Anna B" last="Ohlsson">Anna B. Ohlsson</name></noCountry><country name="Suède"><noRegion><name sortKey="Christiernin, Maria" sort="Christiernin, Maria" uniqKey="Christiernin M" first="Maria" last="Christiernin">Maria Christiernin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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