Expression of poplar chitinase in tomato leads to inhibition of development in colorado potato beetle.
Identifieur interne : 003E20 ( Main/Exploration ); précédent : 003E19; suivant : 003E21Expression of poplar chitinase in tomato leads to inhibition of development in colorado potato beetle.
Auteurs : Susan D. Lawrence [États-Unis] ; Nicole G. NovakSource :
- Biotechnology letters [ 0141-5492 ] ; 2006.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), ARN des plantes (métabolisme), Animaux (MeSH), Chitinase (génétique), Chitinase (métabolisme), Coléoptères (croissance et développement), Feuilles de plante (enzymologie), Feuilles de plante (génétique), Feuilles de plante (parasitologie), Lutte biologique contre les nuisibles (méthodes), Lycopersicon esculentum (enzymologie), Lycopersicon esculentum (génétique), Lycopersicon esculentum (parasitologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Solanum tuberosum (parasitologie), Technique de Northern (MeSH), Technique de Western (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Coléoptères.
- enzymologie : Feuilles de plante, Lycopersicon esculentum.
- génétique : ARN des plantes, Chitinase, Feuilles de plante, Lycopersicon esculentum, Protéines végétales.
- métabolisme : ARN des plantes, Chitinase, Protéines végétales.
- méthodes : Lutte biologique contre les nuisibles.
- parasitologie : Feuilles de plante, Lycopersicon esculentum, Solanum tuberosum.
- Animaux, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Technique de Northern, Technique de Western, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Animals (MeSH), Blotting, Northern (MeSH), Blotting, Western (MeSH), Chitinases (genetics), Chitinases (metabolism), Coleoptera (growth & development), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Lycopersicon esculentum (enzymology), Lycopersicon esculentum (genetics), Lycopersicon esculentum (parasitology), Pest Control, Biological (methods), Plant Leaves (enzymology), Plant Leaves (genetics), Plant Leaves (parasitology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), RNA, Plant (genetics), RNA, Plant (metabolism), Solanum tuberosum (parasitology).
- MESH :
- chemical , genetics : Chitinases, Plant Proteins, RNA, Plant.
- chemical , metabolism : Chitinases, Plant Proteins, RNA, Plant.
- enzymology : Lycopersicon esculentum, Plant Leaves.
- genetics : Lycopersicon esculentum, Plant Leaves.
- growth & development : Coleoptera.
- methods : Pest Control, Biological.
- parasitology : Lycopersicon esculentum, Plant Leaves, Solanum tuberosum.
- Animals, Blotting, Northern, Blotting, Western, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Plants, Genetically Modified.
Abstract
The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado potato beetle [CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect bioassay within 3-4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge this is the first plant chitinase that retards development of an insect pest.
DOI: 10.1007/s10529-006-0022-7
PubMed: 16614898
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Novak</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="ISSN">0141-5492</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Chitinases (genetics)</term><term>Chitinases (metabolism)</term><term>Coleoptera (growth & development)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Lycopersicon esculentum (enzymology)</term><term>Lycopersicon esculentum (genetics)</term><term>Lycopersicon esculentum (parasitology)</term><term>Pest Control, Biological (methods)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (parasitology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term><term>Solanum tuberosum (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>Animaux (MeSH)</term><term>Chitinase (génétique)</term><term>Chitinase (métabolisme)</term><term>Coléoptères (croissance et développement)</term><term>Feuilles de plante (enzymologie)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (parasitologie)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Lycopersicon esculentum (enzymologie)</term><term>Lycopersicon esculentum (génétique)</term><term>Lycopersicon esculentum (parasitologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Solanum tuberosum (parasitologie)</term><term>Technique de Northern (MeSH)</term><term>Technique de Western (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chitinases</term><term>Plant Proteins</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chitinases</term><term>Plant Proteins</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coléoptères</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Feuilles de plante</term><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lycopersicon esculentum</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Coleoptera</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Chitinase</term><term>Feuilles de plante</term><term>Lycopersicon esculentum</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>Chitinase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Feuilles de plante</term><term>Lycopersicon esculentum</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Plant Leaves</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Northern</term><term>Blotting, Western</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term><term>Technique de Northern</term><term>Technique de Western</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado potato beetle [CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect bioassay within 3-4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge this is the first plant chitinase that retards development of an insect pest.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16614898</PMID><DateCompleted><Year>2006</Year><Month>11</Month><Day>27</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0141-5492</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>8</Issue><PubDate><Year>2006</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Expression of poplar chitinase in tomato leads to inhibition of development in colorado potato beetle.</ArticleTitle><Pagination><MedlinePgn>593-9</MedlinePgn></Pagination><Abstract><AbstractText>The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado potato beetle [CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect bioassay within 3-4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge this is the first plant chitinase that retards development of an insect pest.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lawrence</LastName><ForeName>Susan D</ForeName><Initials>SD</Initials><AffiliationInfo><Affiliation>United States Department of Agriculture, Agricultural Research Service, Insect Biocontrol Laboratory, BARC-West, Bldg. 011A, Room 214, Beltsville, MD 20705, USA. Lawrencs@ba.ars.usda.gov</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Novak</LastName><ForeName>Nicole G</ForeName><Initials>NG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="C089149">Win6 protein, Populus trichocarpa</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001517" MajorTopicYN="N">Coleoptera</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>01</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>4</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>12</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>4</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16614898</ArticleId><ArticleId IdType="doi">10.1007/s10529-006-0022-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Novak, Nicole G" sort="Novak, Nicole G" uniqKey="Novak N" first="Nicole G" last="Novak">Nicole G. Novak</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Lawrence, Susan D" sort="Lawrence, Susan D" uniqKey="Lawrence S" first="Susan D" last="Lawrence">Susan D. Lawrence</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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