PoplarV1, Main, Exploration, bibRecord, 003866

Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.

Identifieur interne : 003866 ( Main/Exploration ); précédent : 003865; suivant : 003867

Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.

Auteurs : Ingrid Langer [Autriche] ; Doris Krpata ; Ursula Peintner ; Walter W. Wenzel ; Peter Schweiger

Source :

Mycorrhiza [ 0940-6360 ] ; 2008.

RBID : pubmed:18594875

Descripteurs français

KwdFr :
- Basidiomycota (classification), Basidiomycota (croissance et développement), Basidiomycota (métabolisme), Europe (MeSH), Milieux de culture (composition chimique), Mycorhizes (métabolisme), Populus (microbiologie), Populus (métabolisme), Racines de plante (microbiologie), Racines de plante (métabolisme), Symbiose (MeSH).
MESH :
- composition chimique : Basidiomycota, Milieux de culture.
- croissance et développement : Basidiomycota.
- microbiologie : Populus, Racines de plante.
- métabolisme : Basidiomycota, Mycorhizes, Populus, Racines de plante.
- Europe, Symbiose.

English descriptors

KwdEn :
- Basidiomycota (classification), Basidiomycota (growth & development), Basidiomycota (metabolism), Culture Media (chemistry), Europe (MeSH), Mycorrhizae (metabolism), Plant Roots (metabolism), Plant Roots (microbiology), Populus (metabolism), Populus (microbiology), Symbiosis (MeSH).
MESH :
- chemical , chemistry : Culture Media.
- geographic : Europe.
- classification : Basidiomycota.
- growth & development : Basidiomycota.
- metabolism : Basidiomycota, Mycorrhizae, Plant Roots, Populus.
- microbiology : Plant Roots, Populus.
- Symbiosis.

Abstract

The effect of various media formulations on in vitro ectomycorrhizal synthesis of identified fungal strains with European aspen (Populus tremula L.) was tested in Petri dishes. Pre-grown seedlings were transferred to various nutrient media and inoculated with Paxillus involutus isolates using modified sandwich techniques. Mycorrhiza formation was evaluated macroscopically and further confirmed by microscopic examination of semi-thin sections for anatomical features of the mantle and the Hartig net. Standard media formulations did not support successful ectomycorrhiza formation because of either very poor plant survival (below 20%) or impaired fungal growth. The inclusion of micronutrients and vitamins in a Melin Norkrans (MMN)-based medium increased plant survival rate to above 60% and supported successful mycorrhizal synthesis. P. involutus isolates formed mycorrhizas with a characteristic Hartig net restricted to the epidermis. Mantle density and thickness varied depending on the isolate. In a follow-up experiment, the adapted medium supported successful ectomycorrhiza formation by various Laccaria and Hebeloma isolates. Our results show that an exogenous supply of vitamins and micronutrients in the medium was a prerequisite for successful mycorrhization of P. tremula in vitro in Petri dishes.

DOI: 10.1007/s00572-008-0182-5
PubMed: 18594875

Affiliations:

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Le document en format XML

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<term>Culture Media (chemistry)</term>
<term>Europe (MeSH)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Plant Roots (metabolism)</term>
<term>Plant Roots (microbiology)</term>
<term>Populus (metabolism)</term>
<term>Populus (microbiology)</term>
<term>Symbiosis (MeSH)</term>
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<term>Europe (MeSH)</term>
<term>Milieux de culture (composition chimique)</term>
<term>Mycorhizes (métabolisme)</term>
<term>Populus (microbiologie)</term>
<term>Populus (métabolisme)</term>
<term>Racines de plante (microbiologie)</term>
<term>Racines de plante (métabolisme)</term>
<term>Symbiose (MeSH)</term>
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<term>Racines de plante</term>
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<front><div type="abstract" xml:lang="en">The effect of various media formulations on in vitro ectomycorrhizal synthesis of identified fungal strains with European aspen (Populus tremula L.) was tested in Petri dishes. Pre-grown seedlings were transferred to various nutrient media and inoculated with Paxillus involutus isolates using modified sandwich techniques. Mycorrhiza formation was evaluated macroscopically and further confirmed by microscopic examination of semi-thin sections for anatomical features of the mantle and the Hartig net. Standard media formulations did not support successful ectomycorrhiza formation because of either very poor plant survival (below 20%) or impaired fungal growth. The inclusion of micronutrients and vitamins in a Melin Norkrans (MMN)-based medium increased plant survival rate to above 60% and supported successful mycorrhizal synthesis. P. involutus isolates formed mycorrhizas with a characteristic Hartig net restricted to the epidermis. Mantle density and thickness varied depending on the isolate. In a follow-up experiment, the adapted medium supported successful ectomycorrhiza formation by various Laccaria and Hebeloma isolates. Our results show that an exogenous supply of vitamins and micronutrients in the medium was a prerequisite for successful mycorrhization of P. tremula in vitro in Petri dishes.</div>
</front>
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<Abstract><AbstractText>The effect of various media formulations on in vitro ectomycorrhizal synthesis of identified fungal strains with European aspen (Populus tremula L.) was tested in Petri dishes. Pre-grown seedlings were transferred to various nutrient media and inoculated with Paxillus involutus isolates using modified sandwich techniques. Mycorrhiza formation was evaluated macroscopically and further confirmed by microscopic examination of semi-thin sections for anatomical features of the mantle and the Hartig net. Standard media formulations did not support successful ectomycorrhiza formation because of either very poor plant survival (below 20%) or impaired fungal growth. The inclusion of micronutrients and vitamins in a Melin Norkrans (MMN)-based medium increased plant survival rate to above 60% and supported successful mycorrhizal synthesis. P. involutus isolates formed mycorrhizas with a characteristic Hartig net restricted to the epidermis. Mantle density and thickness varied depending on the isolate. In a follow-up experiment, the adapted medium supported successful ectomycorrhiza formation by various Laccaria and Hebeloma isolates. Our results show that an exogenous supply of vitamins and micronutrients in the medium was a prerequisite for successful mycorrhization of P. tremula in vitro in Petri dishes.</AbstractText>
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Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.

Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki