[Characterization of root nodule and rhizobium of a Leguminosae ephemeral plant--Trigonella arcuata C. A. Mey in Xinjiang].
Identifieur interne : 003749 ( Main/Exploration ); précédent : 003748; suivant : 003750[Characterization of root nodule and rhizobium of a Leguminosae ephemeral plant--Trigonella arcuata C. A. Mey in Xinjiang].
Auteurs : Tianyu You [République populaire de Chine] ; Zhi Tan ; Lili Gu ; Xiuming Li ; Shixiang Yao ; Haiyan Lan ; Fuchun ZhangSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2008.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Antibactériens (pharmacologie), Chine (MeSH), Nodules racinaires de plante (croissance et développement), Nodules racinaires de plante (ultrastructure), Phylogenèse (MeSH), Rhizobium (effets des médicaments et des substances chimiques), Rhizobium (génétique), Rhizobium (isolement et purification), Rhizobium (physiologie), Stress physiologique (MeSH), Température (MeSH), Trigonella (croissance et développement), Trigonella (ultrastructure).
- MESH :
- croissance et développement : Nodules racinaires de plante, Trigonella.
- effets des médicaments et des substances chimiques : Rhizobium.
- génétique : ARN ribosomique 16S, Rhizobium.
- isolement et purification : Rhizobium.
- pharmacologie : Antibactériens.
- physiologie : Rhizobium.
- Chine, Nodules racinaires de plante, Phylogenèse, Stress physiologique, Température, Trigonella.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (pharmacology), China (MeSH), Phylogeny (MeSH), RNA, Ribosomal, 16S (genetics), Rhizobium (drug effects), Rhizobium (genetics), Rhizobium (isolation & purification), Rhizobium (physiology), Root Nodules, Plant (growth & development), Root Nodules, Plant (ultrastructure), Stress, Physiological (MeSH), Temperature (MeSH), Trigonella (growth & development), Trigonella (ultrastructure).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical , pharmacology : Anti-Bacterial Agents.
- geographic : China.
- drug effects : Rhizobium.
- genetics : Rhizobium.
- growth & development : Root Nodules, Plant, Trigonella.
- isolation & purification : Rhizobium.
- physiology : Rhizobium.
- ultrastructure : Root Nodules, Plant, Trigonella.
- Phylogeny, Stress, Physiological, Temperature.
Abstract
OBJECTIVE
We studied root nodule proliferation, nodule microstructure, genetic cluster and stress resistance of the rhizobium of Trigonella arcuata.
METHODS
We characterized root nodule and rhizobium with various soil matrixes cultivation, paraffin section, resin semi-ultrathin section techniques, and 16S rRNA gene cluster analysis.
RESULTS
(1) Plants grew in mixed soil (nutritious garden soil: poplar zone soil: desert sands = l:1:1), had the most nodule proliferation and bore the most pods. The shapes of nodule were palm- or ginger-like; (2) Microstructure of the nodule revealed five different parts differentiated within the nodule: epidermis (E), cortex (C), vascular bundle (VB), infected cells (IC) and uninfected cells (UIC); (3) Genetic cluster analysis of the full length 16S rRNA gene sequence (1377 bp) indicated that the rhizobium isolated shared the highest identities with Sinorhizobium meliloti; (4) The rhizobium could grow between 4 and 60 degrees C (20 min), pH 6.0-12.0 and 0-2% NaCl. For the antibiotic sensitivity, the rhizobium could not grow normally in medium with 25 microg/mL Kanamycin, Streptomycin or Cephalothin, except for 100 microg/mL Ampicillin.
CONCLUSION
Good conditions of soil matrixes were important for nodulation of T. arcuata; A large quantity of cells in fascicular nodules were infected by rhizobia; 16S rRNA gene sequence of T. arcuata shared the highest identities with that of Sinorhizobium meliloti, and this strain was able to tolerate relatively higher temperature and alkalin.
PubMed: 18837370
Affiliations:
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Mey in Xinjiang].</title><author><name sortKey="You, Tianyu" sort="You, Tianyu" uniqKey="You T" first="Tianyu" last="You">Tianyu You</name><affiliation wicri:level="1"><nlm:affiliation>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. yty2002@163.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046</wicri:regionArea><wicri:noRegion>Urumqi 830046</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Zhi" sort="Tan, Zhi" uniqKey="Tan Z" first="Zhi" last="Tan">Zhi Tan</name></author><author><name sortKey="Gu, Lili" sort="Gu, Lili" uniqKey="Gu L" first="Lili" last="Gu">Lili Gu</name></author><author><name sortKey="Li, Xiuming" sort="Li, Xiuming" uniqKey="Li X" first="Xiuming" last="Li">Xiuming Li</name></author><author><name sortKey="Yao, Shixiang" sort="Yao, Shixiang" uniqKey="Yao S" first="Shixiang" last="Yao">Shixiang Yao</name></author><author><name sortKey="Lan, Haiyan" sort="Lan, Haiyan" uniqKey="Lan H" first="Haiyan" last="Lan">Haiyan Lan</name></author><author><name sortKey="Zhang, Fuchun" sort="Zhang, Fuchun" uniqKey="Zhang F" first="Fuchun" last="Zhang">Fuchun Zhang</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta microbiologica Sinica</title><idno type="ISSN">0001-6209</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (pharmacology)</term><term>China (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Rhizobium (drug effects)</term><term>Rhizobium (genetics)</term><term>Rhizobium (isolation & purification)</term><term>Rhizobium (physiology)</term><term>Root Nodules, Plant (growth & development)</term><term>Root Nodules, Plant (ultrastructure)</term><term>Stress, Physiological (MeSH)</term><term>Temperature (MeSH)</term><term>Trigonella (growth & development)</term><term>Trigonella (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (génétique)</term><term>Antibactériens (pharmacologie)</term><term>Chine (MeSH)</term><term>Nodules racinaires de plante (croissance et développement)</term><term>Nodules racinaires de plante (ultrastructure)</term><term>Phylogenèse (MeSH)</term><term>Rhizobium (effets des médicaments et des substances chimiques)</term><term>Rhizobium (génétique)</term><term>Rhizobium (isolement et purification)</term><term>Rhizobium (physiologie)</term><term>Stress physiologique (MeSH)</term><term>Température (MeSH)</term><term>Trigonella (croissance et développement)</term><term>Trigonella (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Nodules racinaires de plante</term><term>Trigonella</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Root Nodules, Plant</term><term>Trigonella</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Root Nodules, Plant</term><term>Trigonella</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Phylogeny</term><term>Stress, Physiological</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chine</term><term>Nodules racinaires de plante</term><term>Phylogenèse</term><term>Stress physiologique</term><term>Température</term><term>Trigonella</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>We studied root nodule proliferation, nodule microstructure, genetic cluster and stress resistance of the rhizobium of Trigonella arcuata.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>We characterized root nodule and rhizobium with various soil matrixes cultivation, paraffin section, resin semi-ultrathin section techniques, and 16S rRNA gene cluster analysis.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>(1) Plants grew in mixed soil (nutritious garden soil: poplar zone soil: desert sands = l:1:1), had the most nodule proliferation and bore the most pods. The shapes of nodule were palm- or ginger-like; (2) Microstructure of the nodule revealed five different parts differentiated within the nodule: epidermis (E), cortex (C), vascular bundle (VB), infected cells (IC) and uninfected cells (UIC); (3) Genetic cluster analysis of the full length 16S rRNA gene sequence (1377 bp) indicated that the rhizobium isolated shared the highest identities with Sinorhizobium meliloti; (4) The rhizobium could grow between 4 and 60 degrees C (20 min), pH 6.0-12.0 and 0-2% NaCl. For the antibiotic sensitivity, the rhizobium could not grow normally in medium with 25 microg/mL Kanamycin, Streptomycin or Cephalothin, except for 100 microg/mL Ampicillin.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Good conditions of soil matrixes were important for nodulation of T. arcuata; A large quantity of cells in fascicular nodules were infected by rhizobia; 16S rRNA gene sequence of T. arcuata shared the highest identities with that of Sinorhizobium meliloti, and this strain was able to tolerate relatively higher temperature and alkalin.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18837370</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>15</Day></DateCompleted><DateRevised><Year>2012</Year><Month>04</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>48</Volume><Issue>7</Issue><PubDate><Year>2008</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Characterization of root nodule and rhizobium of a Leguminosae ephemeral plant--Trigonella arcuata C. A. Mey in Xinjiang].</ArticleTitle><Pagination><MedlinePgn>917-23</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">We studied root nodule proliferation, nodule microstructure, genetic cluster and stress resistance of the rhizobium of Trigonella arcuata.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We characterized root nodule and rhizobium with various soil matrixes cultivation, paraffin section, resin semi-ultrathin section techniques, and 16S rRNA gene cluster analysis.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">(1) Plants grew in mixed soil (nutritious garden soil: poplar zone soil: desert sands = l:1:1), had the most nodule proliferation and bore the most pods. The shapes of nodule were palm- or ginger-like; (2) Microstructure of the nodule revealed five different parts differentiated within the nodule: epidermis (E), cortex (C), vascular bundle (VB), infected cells (IC) and uninfected cells (UIC); (3) Genetic cluster analysis of the full length 16S rRNA gene sequence (1377 bp) indicated that the rhizobium isolated shared the highest identities with Sinorhizobium meliloti; (4) The rhizobium could grow between 4 and 60 degrees C (20 min), pH 6.0-12.0 and 0-2% NaCl. For the antibiotic sensitivity, the rhizobium could not grow normally in medium with 25 microg/mL Kanamycin, Streptomycin or Cephalothin, except for 100 microg/mL Ampicillin.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Good conditions of soil matrixes were important for nodulation of T. arcuata; A large quantity of cells in fascicular nodules were infected by rhizobia; 16S rRNA gene sequence of T. arcuata shared the highest identities with that of Sinorhizobium meliloti, and this strain was able to tolerate relatively higher temperature and alkalin.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>You</LastName><ForeName>Tianyu</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. yty2002@163.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Zhi</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Lili</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiuming</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Shixiang</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lan</LastName><ForeName>Haiyan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Fuchun</ForeName><Initials>F</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053204" MajorTopicYN="N">Root Nodules, Plant</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029910" MajorTopicYN="N">Trigonella</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18837370</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Gu, Lili" sort="Gu, Lili" uniqKey="Gu L" first="Lili" last="Gu">Lili Gu</name><name sortKey="Lan, Haiyan" sort="Lan, Haiyan" uniqKey="Lan H" first="Haiyan" last="Lan">Haiyan Lan</name><name sortKey="Li, Xiuming" sort="Li, Xiuming" uniqKey="Li X" first="Xiuming" last="Li">Xiuming Li</name><name sortKey="Tan, Zhi" sort="Tan, Zhi" uniqKey="Tan Z" first="Zhi" last="Tan">Zhi Tan</name><name sortKey="Yao, Shixiang" sort="Yao, Shixiang" uniqKey="Yao S" first="Shixiang" last="Yao">Shixiang Yao</name><name sortKey="Zhang, Fuchun" sort="Zhang, Fuchun" uniqKey="Zhang F" first="Fuchun" last="Zhang">Fuchun Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="You, Tianyu" sort="You, Tianyu" uniqKey="You T" first="Tianyu" last="You">Tianyu You</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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