The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis.
Identifieur interne : 003085 ( Main/Exploration ); précédent : 003084; suivant : 003086The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis.
Auteurs : Seiji Nakagame [Canada] ; Richard P. Chandra ; Jack N. SaddlerSource :
- Biotechnology and bioengineering [ 1097-0290 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Carbohydrates, Proteins.
- chemical , chemistry : Lignin.
- chemical , metabolism : Cellulose, Lignin.
- Biomass, Biotransformation, Hydrolysis, Pinus, Populus, Zea mays.
Abstract
The influence of the residual lignin remaining in the cellulosic rich component of pretreated lignocellulosic substrates on subsequent enzymatic hydrolysis was assessed. Twelve lignin preparations were isolated by two isolation methods (protease treated lignin (PTL) and cellulolytic enzymatic lignin (CEL)) from three types of biomass (corn stover, poplar, and lodgepole pine) that had been pretreated by two processes (steam and organosolv pretreatments). Comparative analysis of the isolated lignin showed that the CEL contained lower amounts of carbohydrates and protein than did the PTL and that the isolated lignin from corn stover contained more carbohydrates than did the lignin derived from the poplar and lodgepole pine. The lower yields of acid insoluble lignin (AIL) obtained from the corn stover when using the PTL method indicated that the lignin from the corn stover had a higher hydrophilicity than did the lignin from the poplar and lodgepole pine. The isolated lignin preparations were added to the reaction mixture containing crystalline cellulose (Avicel) and their possible effects on enzymatic hydrolysis were assessed. It was apparent that the lignin isolated from lodgepole pine and steam pretreated poplar decreased the hydrolysis yields of Avicel, whereas the other isolated lignins did not appear to decrease the hydrolysis yields significantly. The hydrolysis yields of the pretreated lignocellulose and those of Avicel containing the PTL showed good correlation, indicating that the nature of the residual lignin obtained after pretreatment significantly influenced hydrolysis.
DOI: 10.1002/bit.22626
PubMed: 19998278
Affiliations:
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Chandra</name></author><author><name sortKey="Saddler, Jack N" sort="Saddler, Jack N" uniqKey="Saddler J" first="Jack N" last="Saddler">Jack N. Saddler</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19998278</idno><idno type="pmid">19998278</idno><idno type="doi">10.1002/bit.22626</idno><idno type="wicri:Area/Main/Corpus">003367</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003367</idno><idno type="wicri:Area/Main/Curation">003367</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003367</idno><idno type="wicri:Area/Main/Exploration">003367</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis.</title><author><name sortKey="Nakagame, Seiji" sort="Nakagame, Seiji" uniqKey="Nakagame S" first="Seiji" last="Nakagame">Seiji Nakagame</name><affiliation wicri:level="4"><nlm:affiliation>Forest Products Biotechnology, University of British Columbia, 2424 Main Mall, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Forest Products Biotechnology, University of British Columbia, 2424 Main Mall, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Chandra, Richard P" sort="Chandra, Richard P" uniqKey="Chandra R" first="Richard P" last="Chandra">Richard P. 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Saddler</name></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="eISSN">1097-0290</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Biotransformation (MeSH)</term><term>Carbohydrates (analysis)</term><term>Cellulose (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Pinus (MeSH)</term><term>Populus (MeSH)</term><term>Proteins (analysis)</term><term>Zea mays (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Biotransformation (MeSH)</term><term>Cellulose (métabolisme)</term><term>Glucides (analyse)</term><term>Hydrolyse (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Pinus (MeSH)</term><term>Populus (MeSH)</term><term>Protéines (analyse)</term><term>Zea mays (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbohydrates</term><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucides</term><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulose</term><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Biotransformation</term><term>Hydrolysis</term><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Biotransformation</term><term>Hydrolyse</term><term>Pinus</term><term>Populus</term><term>Zea mays</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influence of the residual lignin remaining in the cellulosic rich component of pretreated lignocellulosic substrates on subsequent enzymatic hydrolysis was assessed. Twelve lignin preparations were isolated by two isolation methods (protease treated lignin (PTL) and cellulolytic enzymatic lignin (CEL)) from three types of biomass (corn stover, poplar, and lodgepole pine) that had been pretreated by two processes (steam and organosolv pretreatments). Comparative analysis of the isolated lignin showed that the CEL contained lower amounts of carbohydrates and protein than did the PTL and that the isolated lignin from corn stover contained more carbohydrates than did the lignin derived from the poplar and lodgepole pine. The lower yields of acid insoluble lignin (AIL) obtained from the corn stover when using the PTL method indicated that the lignin from the corn stover had a higher hydrophilicity than did the lignin from the poplar and lodgepole pine. The isolated lignin preparations were added to the reaction mixture containing crystalline cellulose (Avicel) and their possible effects on enzymatic hydrolysis were assessed. It was apparent that the lignin isolated from lodgepole pine and steam pretreated poplar decreased the hydrolysis yields of Avicel, whereas the other isolated lignins did not appear to decrease the hydrolysis yields significantly. The hydrolysis yields of the pretreated lignocellulose and those of Avicel containing the PTL showed good correlation, indicating that the nature of the residual lignin obtained after pretreatment significantly influenced hydrolysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19998278</PMID><DateCompleted><Year>2010</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2010</Year><Month>02</Month><Day>26</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1097-0290</ISSN><JournalIssue CitedMedium="Internet"><Volume>105</Volume><Issue>5</Issue><PubDate><Year>2010</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis.</ArticleTitle><Pagination><MedlinePgn>871-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/bit.22626</ELocationID><Abstract><AbstractText>The influence of the residual lignin remaining in the cellulosic rich component of pretreated lignocellulosic substrates on subsequent enzymatic hydrolysis was assessed. Twelve lignin preparations were isolated by two isolation methods (protease treated lignin (PTL) and cellulolytic enzymatic lignin (CEL)) from three types of biomass (corn stover, poplar, and lodgepole pine) that had been pretreated by two processes (steam and organosolv pretreatments). Comparative analysis of the isolated lignin showed that the CEL contained lower amounts of carbohydrates and protein than did the PTL and that the isolated lignin from corn stover contained more carbohydrates than did the lignin derived from the poplar and lodgepole pine. The lower yields of acid insoluble lignin (AIL) obtained from the corn stover when using the PTL method indicated that the lignin from the corn stover had a higher hydrophilicity than did the lignin from the poplar and lodgepole pine. The isolated lignin preparations were added to the reaction mixture containing crystalline cellulose (Avicel) and their possible effects on enzymatic hydrolysis were assessed. It was apparent that the lignin isolated from lodgepole pine and steam pretreated poplar decreased the hydrolysis yields of Avicel, whereas the other isolated lignins did not appear to decrease the hydrolysis yields significantly. The hydrolysis yields of the pretreated lignocellulose and those of Avicel containing the PTL showed good correlation, indicating that the nature of the residual lignin obtained after pretreatment significantly influenced hydrolysis.</AbstractText><CopyrightInformation>(c) 2009 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakagame</LastName><ForeName>Seiji</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Forest Products Biotechnology, University of British Columbia, 2424 Main Mall, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chandra</LastName><ForeName>Richard P</ForeName><Initials>RP</Initials></Author><Author ValidYN="Y"><LastName>Saddler</LastName><ForeName>Jack N</ForeName><Initials>JN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002241" MajorTopicYN="N">Carbohydrates</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19998278</ArticleId><ArticleId IdType="doi">10.1002/bit.22626</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Chandra, Richard P" sort="Chandra, Richard P" uniqKey="Chandra R" first="Richard P" last="Chandra">Richard P. Chandra</name><name sortKey="Saddler, Jack N" sort="Saddler, Jack N" uniqKey="Saddler J" first="Jack N" last="Saddler">Jack N. Saddler</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Nakagame, Seiji" sort="Nakagame, Seiji" uniqKey="Nakagame S" first="Seiji" last="Nakagame">Seiji Nakagame</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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