A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis.
Identifieur interne : 002400 ( Main/Exploration ); précédent : 002399; suivant : 002401A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis.
Auteurs : Vibe M. Gondolf ; Rhea Stoppel ; Berit Ebert ; Carsten Rautengarten ; April Jm Liwanag ; Dominique Loqué ; Henrik V. SchellerSource :
- BMC plant biology [ 1471-2229 ] ; 2014.
Descripteurs français
- KwdFr :
- Arabidopsis (génétique), Arabidopsis (métabolisme), Biocarburants (analyse), Galactanes (métabolisme), Galactose (métabolisme), Paroi cellulaire (métabolisme), Populus (génétique), Populus (métabolisme), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Sélection (MeSH), UDP glucose 4-epimerase (génétique), UDP glucose 4-epimerase (métabolisme), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- analyse : Biocarburants.
- génétique : Arabidopsis, Populus, Protéines d'Arabidopsis, Protéines végétales, UDP glucose 4-epimerase, Végétaux génétiquement modifiés.
- métabolisme : Arabidopsis, Galactanes, Galactose, Paroi cellulaire, Populus, Protéines d'Arabidopsis, Protéines végétales, UDP glucose 4-epimerase, Végétaux génétiquement modifiés.
- Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Sélection.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Biofuels (analysis), Breeding (MeSH), Cell Wall (metabolism), Galactans (metabolism), Galactose (metabolism), Gene Expression Regulation, Plant (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Promoter Regions, Genetic (MeSH), UDPglucose 4-Epimerase (genetics), UDPglucose 4-Epimerase (metabolism).
- MESH :
- chemical , analysis : Biofuels.
- chemical , genetics : Arabidopsis Proteins, Plant Proteins, UDPglucose 4-Epimerase.
- genetics : Arabidopsis, Plants, Genetically Modified, Populus.
- metabolism : Arabidopsis, Arabidopsis Proteins, Cell Wall, Galactans, Galactose, Plant Proteins, Plants, Genetically Modified, Populus, UDPglucose 4-Epimerase.
- Breeding, Gene Expression Regulation, Plant, Promoter Regions, Genetic.
Abstract
BACKGROUND
Engineering of plants with a composition of lignocellulosic biomass that is more suitable for downstream processing is of high interest for next-generation biofuel production. Lignocellulosic biomass contains a high proportion of pentose residues, which are more difficult to convert into fuels than hexoses. Therefore, increasing the hexose/pentose ratio in biomass is one approach for biomass improvement. A genetic engineering approach was used to investigate whether the amount of pectic galactan can be specifically increased in cell walls of Arabidopsis fiber cells, which in turn could provide a potential source of readily fermentable galactose.
RESULTS
First it was tested if overexpression of various plant UDP-glucose 4-epimerases (UGEs) could increase the availability of UDP-galactose and thereby increase the biosynthesis of galactan. Constitutive and tissue-specific expression of a poplar UGE and three Arabidopsis UGEs in Arabidopsis plants could not significantly increase the amount of cell wall bound galactose. We then investigated co-overexpression of AtUGE2 together with the β-1,4-galactan synthase GalS1. Co-overexpression of AtUGE2 and GalS1 led to over 80% increase in cell wall galactose levels in Arabidopsis stems, providing evidence that these proteins work synergistically. Furthermore, AtUGE2 and GalS1 overexpression in combination with overexpression of the NST1 master regulator for secondary cell wall biosynthesis resulted in increased thickness of fiber cell walls in addition to the high cell wall galactose levels. Immunofluorescence microscopy confirmed that the increased galactose was present as β-1,4-galactan in secondary cell walls.
CONCLUSIONS
This approach clearly indicates that simultaneous overexpression of AtUGE2 and GalS1 increases the cell wall galactose to much higher levels than can be achieved by overexpressing either one of these proteins alone. Moreover, the increased galactan content in fiber cells while improving the biomass composition had no impact on plant growth and development and hence on the overall biomass amount. Thus, we could show that the gene stacking approach described here is a promising method to engineer advanced feedstocks for biofuel production.
DOI: 10.1186/s12870-014-0344-x
PubMed: 25492673
PubMed Central: PMC4268804
Affiliations:
Links toward previous steps (curation, corpus...)
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Gondolf</name></author><author><name sortKey="Stoppel, Rhea" sort="Stoppel, Rhea" uniqKey="Stoppel R" first="Rhea" last="Stoppel">Rhea Stoppel</name></author><author><name sortKey="Ebert, Berit" sort="Ebert, Berit" uniqKey="Ebert B" first="Berit" last="Ebert">Berit Ebert</name></author><author><name sortKey="Rautengarten, Carsten" sort="Rautengarten, Carsten" uniqKey="Rautengarten C" first="Carsten" last="Rautengarten">Carsten Rautengarten</name></author><author><name sortKey="Liwanag, April Jm" sort="Liwanag, April Jm" uniqKey="Liwanag A" first="April Jm" last="Liwanag">April Jm Liwanag</name></author><author><name sortKey="Loque, Dominique" sort="Loque, Dominique" uniqKey="Loque D" first="Dominique" last="Loqué">Dominique Loqué</name></author><author><name sortKey="Scheller, Henrik V" sort="Scheller, Henrik V" uniqKey="Scheller H" first="Henrik V" last="Scheller">Henrik V. 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Gondolf</name></author><author><name sortKey="Stoppel, Rhea" sort="Stoppel, Rhea" uniqKey="Stoppel R" first="Rhea" last="Stoppel">Rhea Stoppel</name></author><author><name sortKey="Ebert, Berit" sort="Ebert, Berit" uniqKey="Ebert B" first="Berit" last="Ebert">Berit Ebert</name></author><author><name sortKey="Rautengarten, Carsten" sort="Rautengarten, Carsten" uniqKey="Rautengarten C" first="Carsten" last="Rautengarten">Carsten Rautengarten</name></author><author><name sortKey="Liwanag, April Jm" sort="Liwanag, April Jm" uniqKey="Liwanag A" first="April Jm" last="Liwanag">April Jm Liwanag</name></author><author><name sortKey="Loque, Dominique" sort="Loque, Dominique" uniqKey="Loque D" first="Dominique" last="Loqué">Dominique Loqué</name></author><author><name sortKey="Scheller, Henrik V" sort="Scheller, Henrik V" uniqKey="Scheller H" first="Henrik V" last="Scheller">Henrik V. 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Lignocellulosic biomass contains a high proportion of pentose residues, which are more difficult to convert into fuels than hexoses. Therefore, increasing the hexose/pentose ratio in biomass is one approach for biomass improvement. A genetic engineering approach was used to investigate whether the amount of pectic galactan can be specifically increased in cell walls of Arabidopsis fiber cells, which in turn could provide a potential source of readily fermentable galactose.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>First it was tested if overexpression of various plant UDP-glucose 4-epimerases (UGEs) could increase the availability of UDP-galactose and thereby increase the biosynthesis of galactan. Constitutive and tissue-specific expression of a poplar UGE and three Arabidopsis UGEs in Arabidopsis plants could not significantly increase the amount of cell wall bound galactose. We then investigated co-overexpression of AtUGE2 together with the β-1,4-galactan synthase GalS1. Co-overexpression of AtUGE2 and GalS1 led to over 80% increase in cell wall galactose levels in Arabidopsis stems, providing evidence that these proteins work synergistically. Furthermore, AtUGE2 and GalS1 overexpression in combination with overexpression of the NST1 master regulator for secondary cell wall biosynthesis resulted in increased thickness of fiber cell walls in addition to the high cell wall galactose levels. Immunofluorescence microscopy confirmed that the increased galactose was present as β-1,4-galactan in secondary cell walls.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This approach clearly indicates that simultaneous overexpression of AtUGE2 and GalS1 increases the cell wall galactose to much higher levels than can be achieved by overexpressing either one of these proteins alone. Moreover, the increased galactan content in fiber cells while improving the biomass composition had no impact on plant growth and development and hence on the overall biomass amount. Thus, we could show that the gene stacking approach described here is a promising method to engineer advanced feedstocks for biofuel production.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25492673</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><PubDate><Year>2014</Year><Month>Dec</Month><Day>10</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>344</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-014-0344-x</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Engineering of plants with a composition of lignocellulosic biomass that is more suitable for downstream processing is of high interest for next-generation biofuel production. Lignocellulosic biomass contains a high proportion of pentose residues, which are more difficult to convert into fuels than hexoses. Therefore, increasing the hexose/pentose ratio in biomass is one approach for biomass improvement. A genetic engineering approach was used to investigate whether the amount of pectic galactan can be specifically increased in cell walls of Arabidopsis fiber cells, which in turn could provide a potential source of readily fermentable galactose.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">First it was tested if overexpression of various plant UDP-glucose 4-epimerases (UGEs) could increase the availability of UDP-galactose and thereby increase the biosynthesis of galactan. Constitutive and tissue-specific expression of a poplar UGE and three Arabidopsis UGEs in Arabidopsis plants could not significantly increase the amount of cell wall bound galactose. We then investigated co-overexpression of AtUGE2 together with the β-1,4-galactan synthase GalS1. Co-overexpression of AtUGE2 and GalS1 led to over 80% increase in cell wall galactose levels in Arabidopsis stems, providing evidence that these proteins work synergistically. Furthermore, AtUGE2 and GalS1 overexpression in combination with overexpression of the NST1 master regulator for secondary cell wall biosynthesis resulted in increased thickness of fiber cell walls in addition to the high cell wall galactose levels. Immunofluorescence microscopy confirmed that the increased galactose was present as β-1,4-galactan in secondary cell walls.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This approach clearly indicates that simultaneous overexpression of AtUGE2 and GalS1 increases the cell wall galactose to much higher levels than can be achieved by overexpressing either one of these proteins alone. Moreover, the increased galactan content in fiber cells while improving the biomass composition had no impact on plant growth and development and hence on the overall biomass amount. Thus, we could show that the gene stacking approach described here is a promising method to engineer advanced feedstocks for biofuel production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gondolf</LastName><ForeName>Vibe M</ForeName><Initials>VM</Initials></Author><Author ValidYN="Y"><LastName>Stoppel</LastName><ForeName>Rhea</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ebert</LastName><ForeName>Berit</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Rautengarten</LastName><ForeName>Carsten</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Liwanag</LastName><ForeName>April Jm</ForeName><Initials>AJ</Initials></Author><Author ValidYN="Y"><LastName>Loqué</LastName><ForeName>Dominique</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Scheller</LastName><ForeName>Henrik V</ForeName><Initials>HV</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Plant Biol</MedlineTA><NlmUniqueID>100967807</NlmUniqueID><ISSNLinking>1471-2229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005685">Galactans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant 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MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001947" MajorTopicYN="N">Breeding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005685" MajorTopicYN="N">Galactans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005690" MajorTopicYN="N">Galactose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014534" MajorTopicYN="N">UDPglucose 4-Epimerase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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Gondolf</name><name sortKey="Liwanag, April Jm" sort="Liwanag, April Jm" uniqKey="Liwanag A" first="April Jm" last="Liwanag">April Jm Liwanag</name><name sortKey="Loque, Dominique" sort="Loque, Dominique" uniqKey="Loque D" first="Dominique" last="Loqué">Dominique Loqué</name><name sortKey="Rautengarten, Carsten" sort="Rautengarten, Carsten" uniqKey="Rautengarten C" first="Carsten" last="Rautengarten">Carsten Rautengarten</name><name sortKey="Scheller, Henrik V" sort="Scheller, Henrik V" uniqKey="Scheller H" first="Henrik V" last="Scheller">Henrik V. Scheller</name><name sortKey="Stoppel, Rhea" sort="Stoppel, Rhea" uniqKey="Stoppel R" first="Rhea" last="Stoppel">Rhea Stoppel</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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