PoplarV1, Main, Exploration, bibRecord, 002329

Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.

Identifieur interne : 002329 ( Main/Exploration ); précédent : 002328; suivant : 002330

Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.

Auteurs : Kai Dou [République populaire de Chine] ; Zhiying Wang [République populaire de Chine] ; Rongshu Zhang [République populaire de Chine] ; Na Wang [République populaire de Chine] ; Haijuan Fan [République populaire de Chine] ; Guiping Diao [République populaire de Chine] ; Zhihua Liu [République populaire de Chine]

Source :

Microbiological research [ 1618-0623 ] ; 2014.

RBID : pubmed:24932881

Descripteurs français

English descriptors

KwdEn :
- Alternaria (MeSH), Amino Acid Sequence (MeSH), Aspartic Acid Proteases (genetics), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Real-Time Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Trichoderma (enzymology), Trichoderma (genetics).
MESH :
- chemical , genetics : Aspartic Acid Proteases, DNA, Fungal, Fungal Proteins.
- enzymology : Trichoderma.
- genetics : Escherichia coli, Trichoderma.
- Alternaria, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Fungal, Molecular Sequence Data, Phylogeny, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA.

Abstract

Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata.

DOI: 10.1016/j.micres.2014.04.006
PubMed: 24932881

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
<term>Aspartic Acid Proteases (genetics)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (genetics)</term>
<term>Escherichia coli (genetics)</term>
<term>Fungal Proteins (genetics)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Trichoderma (enzymology)</term>
<term>Trichoderma (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term>
<term>Alternaria (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Aspartic acid proteases (génétique)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Escherichia coli (génétique)</term>
<term>Phylogenèse (MeSH)</term>
<term>Protéines fongiques (génétique)</term>
<term>Réaction de polymérisation en chaine en temps réel (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Trichoderma (enzymologie)</term>
<term>Trichoderma (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aspartic Acid Proteases</term>
<term>DNA, Fungal</term>
<term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Trichoderma</term>
</keywords>
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</keywords>
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<term>Aspartic acid proteases</term>
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<term>Trichoderma</term>
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<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
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<term>Analyse de séquence d'ADN</term>
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<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Régulation de l'expression des gènes fongiques</term>
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<front><div type="abstract" xml:lang="en">Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata. </div>
</front>
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<Abstract><AbstractText>Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata. </AbstractText>
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Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.

Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.

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