Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.
Identifieur interne : 002329 ( Main/Exploration ); précédent : 002328; suivant : 002330Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperellum ACCC30536.
Auteurs : Kai Dou [République populaire de Chine] ; Zhiying Wang [République populaire de Chine] ; Rongshu Zhang [République populaire de Chine] ; Na Wang [République populaire de Chine] ; Haijuan Fan [République populaire de Chine] ; Guiping Diao [République populaire de Chine] ; Zhihua Liu [République populaire de Chine]Source :
- Microbiological research [ 1618-0623 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Alternaria (MeSH), Analyse de séquence d'ADN (MeSH), Aspartic acid proteases (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Phylogenèse (MeSH), Protéines fongiques (génétique), Réaction de polymérisation en chaine en temps réel (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Trichoderma (enzymologie), Trichoderma (génétique).
- MESH :
- enzymologie : Trichoderma.
- génétique : ADN fongique, Aspartic acid proteases, Escherichia coli, Protéines fongiques, Trichoderma.
- Alternaria, Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, Réaction de polymérisation en chaine en temps réel, Régulation de l'expression des gènes fongiques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Alternaria (MeSH), Amino Acid Sequence (MeSH), Aspartic Acid Proteases (genetics), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Real-Time Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Trichoderma (enzymology), Trichoderma (genetics).
- MESH :
- chemical , genetics : Aspartic Acid Proteases, DNA, Fungal, Fungal Proteins.
- enzymology : Trichoderma.
- genetics : Escherichia coli, Trichoderma.
- Alternaria, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Fungal, Molecular Sequence Data, Phylogeny, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata.
DOI: 10.1016/j.micres.2014.04.006
PubMed: 24932881
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Amino Acid Sequence (MeSH)</term>
<term>Aspartic Acid Proteases (genetics)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (genetics)</term>
<term>Escherichia coli (genetics)</term>
<term>Fungal Proteins (genetics)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Trichoderma (enzymology)</term>
<term>Trichoderma (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term>
<term>Alternaria (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Aspartic acid proteases (génétique)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Escherichia coli (génétique)</term>
<term>Phylogenèse (MeSH)</term>
<term>Protéines fongiques (génétique)</term>
<term>Réaction de polymérisation en chaine en temps réel (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Trichoderma (enzymologie)</term>
<term>Trichoderma (génétique)</term>
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<term>DNA, Fungal</term>
<term>Fungal Proteins</term>
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<term>Trichoderma</term>
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<term>Amino Acid Sequence</term>
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<term>Gene Expression Regulation, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
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<front><div type="abstract" xml:lang="en">Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata. </div>
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<Abstract><AbstractText>Proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol. In this study, the coding sequence and promoter region of the novel aspartic protease gene Asp55 were cloned from strain Trichoderma asperellum ACCC30536. Many cis-elements involved in phytopathogenic and environmental stress responses were identified in the Asp55 promoter region and may be recognized by MYB or WRKY transcription factors. The expression pattern of Asp55 under eight culture conditions was investigated by RT-qPCR. The expression level of Asp55 was up-regulated by poplar stem powder, Alternaria alternata cell wall fragments and A. alternata fermentation liquid, while it was down-regulated by carbon and nitrogen source starvation, and by powdered poplar leaves and roots. Additionally, the expression patterns of 15 genes encoding MYB transcription factors (Myb1 to Myb15) were also analyzed by RT-qPCR. Myb2 showed the most similar expression pattern with Asp55. The cDNA of Asp55 was expressed in Escherichia coli BL21, and recombinant ASP55 (rASP55) was purified. The purified rASP55 was evaluated for enzymatic activity and showed inhibitory effect on phytopathogenic A. alternata. </AbstractText>
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<ArticleIdList><ArticleId IdType="pubmed">24932881</ArticleId>
<ArticleId IdType="pii">S0944-5013(14)00061-5</ArticleId>
<ArticleId IdType="doi">10.1016/j.micres.2014.04.006</ArticleId>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Dou, Kai" sort="Dou, Kai" uniqKey="Dou K" first="Kai" last="Dou">Kai Dou</name>
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<name sortKey="Diao, Guiping" sort="Diao, Guiping" uniqKey="Diao G" first="Guiping" last="Diao">Guiping Diao</name>
<name sortKey="Fan, Haijuan" sort="Fan, Haijuan" uniqKey="Fan H" first="Haijuan" last="Fan">Haijuan Fan</name>
<name sortKey="Liu, Zhihua" sort="Liu, Zhihua" uniqKey="Liu Z" first="Zhihua" last="Liu">Zhihua Liu</name>
<name sortKey="Wang, Na" sort="Wang, Na" uniqKey="Wang N" first="Na" last="Wang">Na Wang</name>
<name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name>
<name sortKey="Zhang, Rongshu" sort="Zhang, Rongshu" uniqKey="Zhang R" first="Rongshu" last="Zhang">Rongshu Zhang</name>
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