Differential regulation of cytokinin oxidase genes and cytokinin-induced auxin biosynthesis by cellular cytokinin level in transgenic poplars.
Identifieur interne : 002286 ( Main/Exploration ); précédent : 002285; suivant : 002287Differential regulation of cytokinin oxidase genes and cytokinin-induced auxin biosynthesis by cellular cytokinin level in transgenic poplars.
Auteurs : Young Im Choi [Corée du Sud] ; Eun Woon Noh ; Hae Jung Kim ; Woong June ParkSource :
- Plant cell reports [ 1432-203X ] ; 2014.
Descripteurs français
- KwdFr :
- Acides indolacétiques (métabolisme), Cytokinine (métabolisme), Cytokinine (pharmacologie), Oxidoreductases (génétique), Oxidoreductases (métabolisme), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes végétaux (génétique), Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- effets des médicaments et des substances chimiques : Populus, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
- génétique : Oxidoreductases, Populus, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
- métabolisme : Acides indolacétiques, Cytokinine, Oxidoreductases, Populus, Végétaux génétiquement modifiés.
- pharmacologie : Cytokinine.
English descriptors
- KwdEn :
- Cytokinins (metabolism), Cytokinins (pharmacology), Gene Expression Regulation, Plant (drug effects), Gene Expression Regulation, Plant (genetics), Indoleacetic Acids (metabolism), Oxidoreductases (genetics), Oxidoreductases (metabolism), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (drug effects), Populus (genetics), Populus (metabolism).
- MESH :
- chemical , genetics : Oxidoreductases.
- chemical , metabolism : Cytokinins, Indoleacetic Acids, Oxidoreductases.
- chemical , pharmacology : Cytokinins.
- drug effects : Gene Expression Regulation, Plant, Plants, Genetically Modified, Populus.
- genetics : Gene Expression Regulation, Plant, Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
Abstract
KEY MESSAGE
The present work with transgenic poplar lines producing varying levels of trans -zeatin suggests the existence of a switching threshold for triggering ckx gene expression or suppressing cytokinin-induced auxin. Cytokinins have an important role in growth and developmental processes of plants. Transgenic plants with varying levels of cellular cytokinin are convenient tools for studying its role in morphogenetic as well as molecular responses. In this work, the transgenic lines producing either high level of cellular trans-zeatin (HX lines) or moderate level (MX lines) were compared with regard to their cytokinin oxidase activities and cellular auxin content. The HX lines showed typical cytokinin phenotypes including leafy shoots and spontaneous shoot formation on hormone free medium. In contrast, the MX lines did not show any striking phenotypes. However, in leaf disk culture on hormone free medium, they regenerated roots and subsequently formed shoots from the roots. Determination of cellular IAA content revealed a significant increase in the level in MX lines but not in HX lines. Of nine cytokinin oxidase genes (ckx) examined by qPCR, five were activated in HX lines but not in MX lines. Among them, ckx4 appeared to play a key role in maintaining cellular cytokinin level since it showed more than 1,000-fold increase in HX lines and in the leaf disks of untransformed control exposed to exogenous cytokinins. Although low level of cellular cytokinin did not induce the expression of ckx genes, it appeared to trigger cellular IAA biosynthesis.
DOI: 10.1007/s00299-014-1652-1
PubMed: 25048021
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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poplars.</title><author><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young Im" last="Choi">Young Im Choi</name><affiliation wicri:level="1"><nlm:affiliation>Forest Biotechnology Division, Korea Forest Research Institute, Suwon, Gyeonggi do, 441-847, Republic of Korea, yichoi99@forest.go.kr.</nlm:affiliation><country wicri:rule="url">Corée du Sud</country><wicri:regionArea>Forest Biotechnology Division, Korea Forest Research Institute, Suwon, Gyeonggi do, 441-847, Republic of Korea</wicri:regionArea><wicri:noRegion>Republic of Korea</wicri:noRegion></affiliation></author><author><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun Woon" last="Noh">Eun Woon Noh</name></author><author><name sortKey="Kim, Hae Jung" sort="Kim, Hae Jung" uniqKey="Kim H" first="Hae Jung" last="Kim">Hae Jung Kim</name></author><author><name sortKey="Park, Woong June" sort="Park, Woong June" uniqKey="Park W" first="Woong June" last="Park">Woong June Park</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytokinins (metabolism)</term><term>Cytokinins (pharmacology)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Indoleacetic Acids (metabolism)</term><term>Oxidoreductases (genetics)</term><term>Oxidoreductases (metabolism)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides indolacétiques (métabolisme)</term><term>Cytokinine (métabolisme)</term><term>Cytokinine (pharmacologie)</term><term>Oxidoreductases (génétique)</term><term>Oxidoreductases (métabolisme)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokinins</term><term>Indoleacetic Acids</term><term>Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cytokinins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Oxidoreductases</term><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides indolacétiques</term><term>Cytokinine</term><term>Oxidoreductases</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Cytokinine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b></p><p>The present work with transgenic poplar lines producing varying levels of trans -zeatin suggests the existence of a switching threshold for triggering ckx gene expression or suppressing cytokinin-induced auxin. Cytokinins have an important role in growth and developmental processes of plants. Transgenic plants with varying levels of cellular cytokinin are convenient tools for studying its role in morphogenetic as well as molecular responses. In this work, the transgenic lines producing either high level of cellular trans-zeatin (HX lines) or moderate level (MX lines) were compared with regard to their cytokinin oxidase activities and cellular auxin content. The HX lines showed typical cytokinin phenotypes including leafy shoots and spontaneous shoot formation on hormone free medium. In contrast, the MX lines did not show any striking phenotypes. However, in leaf disk culture on hormone free medium, they regenerated roots and subsequently formed shoots from the roots. Determination of cellular IAA content revealed a significant increase in the level in MX lines but not in HX lines. Of nine cytokinin oxidase genes (ckx) examined by qPCR, five were activated in HX lines but not in MX lines. Among them, ckx4 appeared to play a key role in maintaining cellular cytokinin level since it showed more than 1,000-fold increase in HX lines and in the leaf disks of untransformed control exposed to exogenous cytokinins. Although low level of cellular cytokinin did not induce the expression of ckx genes, it appeared to trigger cellular IAA biosynthesis.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25048021</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>10</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Differential regulation of cytokinin oxidase genes and cytokinin-induced auxin biosynthesis by cellular cytokinin level in transgenic poplars.</ArticleTitle><Pagination><MedlinePgn>1737-44</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-014-1652-1</ELocationID><Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="CONCLUSIONS">The present work with transgenic poplar lines producing varying levels of trans -zeatin suggests the existence of a switching threshold for triggering ckx gene expression or suppressing cytokinin-induced auxin. Cytokinins have an important role in growth and developmental processes of plants. Transgenic plants with varying levels of cellular cytokinin are convenient tools for studying its role in morphogenetic as well as molecular responses. In this work, the transgenic lines producing either high level of cellular trans-zeatin (HX lines) or moderate level (MX lines) were compared with regard to their cytokinin oxidase activities and cellular auxin content. The HX lines showed typical cytokinin phenotypes including leafy shoots and spontaneous shoot formation on hormone free medium. In contrast, the MX lines did not show any striking phenotypes. However, in leaf disk culture on hormone free medium, they regenerated roots and subsequently formed shoots from the roots. Determination of cellular IAA content revealed a significant increase in the level in MX lines but not in HX lines. Of nine cytokinin oxidase genes (ckx) examined by qPCR, five were activated in HX lines but not in MX lines. Among them, ckx4 appeared to play a key role in maintaining cellular cytokinin level since it showed more than 1,000-fold increase in HX lines and in the leaf disks of untransformed control exposed to exogenous cytokinins. Although low level of cellular cytokinin did not induce the expression of ckx genes, it appeared to trigger cellular IAA biosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young Im</ForeName><Initials>YI</Initials><AffiliationInfo><Affiliation>Forest Biotechnology Division, Korea Forest Research Institute, Suwon, Gyeonggi do, 441-847, Republic of Korea, yichoi99@forest.go.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Eun Woon</ForeName><Initials>EW</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hae Jung</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Woong June</ForeName><Initials>WJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>07</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003583">Cytokinins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007210">Indoleacetic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.5.99.12</RegistryNumber><NameOfSubstance UI="C019984">cytokinin oxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003583" MajorTopicYN="N">Cytokinins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007210" MajorTopicYN="N">Indoleacetic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug 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PubStatus="pubmed"><Year>2014</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25048021</ArticleId><ArticleId IdType="doi">10.1007/s00299-014-1652-1</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Symp Soc Exp Biol. 1957;11:118-30</Citation><ArticleIdList><ArticleId IdType="pubmed">13486467</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2000 Jul;23(2):279-84</Citation><ArticleIdList><ArticleId IdType="pubmed">10929121</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:89-118</Citation><ArticleIdList><ArticleId IdType="pubmed">11337393</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2003 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