PoplarV1, Main, Exploration, bibRecord, 002240

Evolution, three-dimensional model and localization of truncated hemoglobin PttTrHb of hybrid aspen.

Identifieur interne : 002240 ( Main/Exploration ); précédent : 002239; suivant : 002241

Evolution, three-dimensional model and localization of truncated hemoglobin PttTrHb of hybrid aspen.

Auteurs : Estelle Dumont [France] ; Soile Jokipii-Lukkari [Finlande] ; Vimal Parkash [Finlande] ; Jaana Vuosku [Finlande] ; Robin Sundström [Finlande] ; Yvonne Nymalm [Finlande] ; Suvi Sutela [Finlande] ; Katariina Taskinen [Finlande] ; Pauli T. Kallio [Suisse] ; Tiina A. Salminen [Finlande] ; Hely H Ggman [Finlande]

Source :

PloS one [ 1932-6203 ] ; 2014.

RBID : pubmed:24520401

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Biological Evolution (MeSH), Gene Expression Regulation, Plant (MeSH), Heme (chemistry), Heme (metabolism), Hybridization, Genetic (MeSH), Models, Molecular (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Leaves (metabolism), Plant Stems (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (metabolism), Protein Transport (MeSH), RNA Interference (MeSH), Sequence Alignment (MeSH), Structural Homology, Protein (MeSH), Truncated Hemoglobins (chemistry), Truncated Hemoglobins (genetics), Truncated Hemoglobins (metabolism).
MESH :
- chemical , chemistry : Heme, Truncated Hemoglobins.
- chemical , genetics : Truncated Hemoglobins.
- chemical , metabolism : Heme, Truncated Hemoglobins.
- genetics : Populus.
- metabolism : Plant Leaves, Plant Stems, Populus.
- Amino Acid Sequence, Biological Evolution, Gene Expression Regulation, Plant, Hybridization, Genetic, Models, Molecular, Molecular Sequence Data, Phylogeny, Plants, Genetically Modified, Protein Transport, RNA Interference, Sequence Alignment, Structural Homology, Protein.

Abstract

Thus far, research on plant hemoglobins (Hbs) has mainly concentrated on symbiotic and non-symbiotic Hbs, and information on truncated Hbs (TrHbs) is scarce. The aim of this study was to examine the origin, structure and localization of the truncated Hb (PttTrHb) of hybrid aspen (Populus tremula L. × tremuloides Michx.), the model system of tree biology. Additionally, we studied the PttTrHb expression in relation to non-symbiotic class1 Hb gene (PttHb1) using RNAi-silenced hybrid aspen lines. Both the phylogenetic analysis and the three-dimensional (3D) model of PttTrHb supported the view that plant TrHbs evolved vertically from a bacterial TrHb. The 3D model suggested that PttTrHb adopts a 2-on-2 sandwich of α-helices and has a Bacillus subtilis -like ligand-binding pocket in which E11Gln and B10Tyr form hydrogen bonds to a ligand. However, due to differences in tunnel cavity and gate residue (E7Ala), it might not show similar ligand-binding kinetics as in Bs-HbO (E7Thr). The immunolocalization showed that PttTrHb protein was present in roots, stems as well as leaves of in vitro -grown hybrid aspens. In mature organs, PttTrHb was predominantly found in the vascular bundles and specifically at the site of lateral root formation, overlapping consistently with areas of nitric oxide (NO) production in plants. Furthermore, the NO donor sodium nitroprusside treatment increased the amount of PttTrHb in stems. The observed PttTrHb localization suggests that PttTrHb plays a role in the NO metabolism.

DOI: 10.1371/journal.pone.0088573
PubMed: 24520401
PubMed Central: PMC3919811

Affiliations:

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Biological Evolution (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Heme (chemistry)</term>
<term>Heme (metabolism)</term>
<term>Hybridization, Genetic (MeSH)</term>
<term>Models, Molecular (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Stems (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Protein Transport (MeSH)</term>
<term>RNA Interference (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Structural Homology, Protein (MeSH)</term>
<term>Truncated Hemoglobins (chemistry)</term>
<term>Truncated Hemoglobins (genetics)</term>
<term>Truncated Hemoglobins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Hybridation génétique (MeSH)</term>
<term>Hème (composition chimique)</term>
<term>Hème (métabolisme)</term>
<term>Hémoglobines tronquées (composition chimique)</term>
<term>Hémoglobines tronquées (génétique)</term>
<term>Hémoglobines tronquées (métabolisme)</term>
<term>Interférence par ARN (MeSH)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Phylogenèse (MeSH)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Similitude structurale de protéines (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Tiges de plante (métabolisme)</term>
<term>Transport des protéines (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Évolution biologique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Heme</term>
<term>Truncated Hemoglobins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Truncated Hemoglobins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heme</term>
<term>Truncated Hemoglobins</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Hème</term>
<term>Hémoglobines tronquées</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hémoglobines tronquées</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term>
<term>Plant Stems</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term>
<term>Hème</term>
<term>Hémoglobines tronquées</term>
<term>Populus</term>
<term>Tiges de plante</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Biological Evolution</term>
<term>Gene Expression Regulation, Plant</term>
<term>Hybridization, Genetic</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Plants, Genetically Modified</term>
<term>Protein Transport</term>
<term>RNA Interference</term>
<term>Sequence Alignment</term>
<term>Structural Homology, Protein</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Données de séquences moléculaires</term>
<term>Hybridation génétique</term>
<term>Interférence par ARN</term>
<term>Modèles moléculaires</term>
<term>Phylogenèse</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Similitude structurale de protéines</term>
<term>Séquence d'acides aminés</term>
<term>Transport des protéines</term>
<term>Végétaux génétiquement modifiés</term>
<term>Évolution biologique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Thus far, research on plant hemoglobins (Hbs) has mainly concentrated on symbiotic and non-symbiotic Hbs, and information on truncated Hbs (TrHbs) is scarce. The aim of this study was to examine the origin, structure and localization of the truncated Hb (PttTrHb) of hybrid aspen (Populus tremula L. × tremuloides Michx.), the model system of tree biology. Additionally, we studied the PttTrHb expression in relation to non-symbiotic class1 Hb gene (PttHb1) using RNAi-silenced hybrid aspen lines. Both the phylogenetic analysis and the three-dimensional (3D) model of PttTrHb supported the view that plant TrHbs evolved vertically from a bacterial TrHb. The 3D model suggested that PttTrHb adopts a 2-on-2 sandwich of α-helices and has a Bacillus subtilis -like ligand-binding pocket in which E11Gln and B10Tyr form hydrogen bonds to a ligand. However, due to differences in tunnel cavity and gate residue (E7Ala), it might not show similar ligand-binding kinetics as in Bs-HbO (E7Thr). The immunolocalization showed that PttTrHb protein was present in roots, stems as well as leaves of in vitro -grown hybrid aspens. In mature organs, PttTrHb was predominantly found in the vascular bundles and specifically at the site of lateral root formation, overlapping consistently with areas of nitric oxide (NO) production in plants. Furthermore, the NO donor sodium nitroprusside treatment increased the amount of PttTrHb in stems. The observed PttTrHb localization suggests that PttTrHb plays a role in the NO metabolism. </div>
</front>
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<Abstract><AbstractText>Thus far, research on plant hemoglobins (Hbs) has mainly concentrated on symbiotic and non-symbiotic Hbs, and information on truncated Hbs (TrHbs) is scarce. The aim of this study was to examine the origin, structure and localization of the truncated Hb (PttTrHb) of hybrid aspen (Populus tremula L. × tremuloides Michx.), the model system of tree biology. Additionally, we studied the PttTrHb expression in relation to non-symbiotic class1 Hb gene (PttHb1) using RNAi-silenced hybrid aspen lines. Both the phylogenetic analysis and the three-dimensional (3D) model of PttTrHb supported the view that plant TrHbs evolved vertically from a bacterial TrHb. The 3D model suggested that PttTrHb adopts a 2-on-2 sandwich of α-helices and has a Bacillus subtilis -like ligand-binding pocket in which E11Gln and B10Tyr form hydrogen bonds to a ligand. However, due to differences in tunnel cavity and gate residue (E7Ala), it might not show similar ligand-binding kinetics as in Bs-HbO (E7Thr). The immunolocalization showed that PttTrHb protein was present in roots, stems as well as leaves of in vitro -grown hybrid aspens. In mature organs, PttTrHb was predominantly found in the vascular bundles and specifically at the site of lateral root formation, overlapping consistently with areas of nitric oxide (NO) production in plants. Furthermore, the NO donor sodium nitroprusside treatment increased the amount of PttTrHb in stems. The observed PttTrHb localization suggests that PttTrHb plays a role in the NO metabolism. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dumont</LastName>
<ForeName>Estelle</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland ; UMR-MD1, Transporteurs Membranaires, Chimiorésistance et Drug-Design, Aix-Marseille Université, Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jokipii-Lukkari</LastName>
<ForeName>Soile</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Parkash</LastName>
<ForeName>Vimal</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Structural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vuosku</LastName>
<ForeName>Jaana</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sundström</LastName>
<ForeName>Robin</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Structural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nymalm</LastName>
<ForeName>Yvonne</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Structural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sutela</LastName>
<ForeName>Suvi</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Taskinen</LastName>
<ForeName>Katariina</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kallio</LastName>
<ForeName>Pauli T</ForeName>
<Initials>PT</Initials>
<AffiliationInfo><Affiliation>Institute of Microbiology, ETH-Zürich, Zürich, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Salminen</LastName>
<ForeName>Tiina A</ForeName>
<Initials>TA</Initials>
<AffiliationInfo><Affiliation>Structural Bioinformatics Laboratory, Department of Biosciences, Åbo Akademi University, Turku, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Häggman</LastName>
<ForeName>Hely</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Oulu, Oulu, Finland.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>02</Month>
<Day>10</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D054793">Truncated Hemoglobins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>42VZT0U6YR</RegistryNumber>
<NameOfSubstance UI="D006418">Heme</NameOfSubstance>
</Chemical>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005075" MajorTopicYN="Y">Biological Evolution</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006418" MajorTopicYN="N">Heme</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006824" MajorTopicYN="Y">Hybridization, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="Y">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040681" MajorTopicYN="N">Structural Homology, Protein</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054793" MajorTopicYN="N">Truncated Hemoglobins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<Month>04</Month>
<Day>08</Day>
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<Month>01</Month>
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<ArticleIdList><ArticleId IdType="pubmed">24520401</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0088573</ArticleId>
<ArticleId IdType="pii">PONE-D-13-14545</ArticleId>
<ArticleId IdType="pmc">PMC3919811</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Methods Enzymol. 1996;266:575-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8743707</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Biochem Biophys. 2007 Jan 1;457(1):85-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17126283</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2008;59(9):2449-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18544611</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Biochem. 1979;48:327-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">382987</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Aug;132(4):1790-800</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12913137</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2005 Mar 11;280(10):9192-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15590662</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1993 Oct 20;233(4):716-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8411177</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2003 Jul;1(4):287-300</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17163905</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2005 Mar;220(5):757-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15517353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>AoB Plants. 2012;2012:pls004</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22479675</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 May 14;279(20):21520-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15016811</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1983;2(12):2143-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16453482</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2008;59(12 ):3443-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18653694</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2009;60(2):409-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19129158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2011 Jan;189(2):415-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21039566</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2011 Oct;28(10):2731-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21546353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Evolution. 1985 Jul;39(4):783-791</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28561359</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Apr 16;316(4):1163-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15044107</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Signal Behav. 2007 Jan;2(1):43-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19516967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2011 Feb;189(3):765-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21073469</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2006 Nov;142(3):1027-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16963525</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol Biochem. 2008 Mar;46(3):371-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18321722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2012 Jul;63(12):4375-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22641422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2000 Jun 1;19(11):2424-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10835341</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10119-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11526234</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Aided Mol Des. 2004 Jun;18(6):401-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15663001</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biotechnol. 2010 Nov;46(3):219-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20563670</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2008;178(3):634-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18312539</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 1999 Jan;4(1):26-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10234267</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 May 13;100(10):5766-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12719529</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2001 Aug 1;20(15):3902-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11483493</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2007 Oct 16;46(41):11440-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17887774</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2007 Apr;189(8):3063-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17293416</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15265-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14657329</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Physiol Plant. 2013 Aug;148(4):457-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23600702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Sci. 2012 Aug;191-192:71-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22682566</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1974 Nov 8;252(5479):169-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4419109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2011 Jun;1814(6):810-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21070893</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2011 Apr;190(2):472-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21244431</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5902-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11959913</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2003 Jun 11;1621(3):299-305</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12787929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2012 Sep;63(15):5581-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22915746</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Signal Behav. 2010 Aug;5(8):1006-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20724824</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Inorg Biochem. 2005 Jan;99(1):97-109</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15598494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10854-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23754374</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2004 Apr;218(6):900-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14716561</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2006 Jul;224(2):246-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16397797</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 1992 Dec;20(6):1203-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1463857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2004 Sep;136(1):2722-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15347796</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS J. 2005 Aug;272(16):4189-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16098200</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Evol. 2006 Feb;62(2):196-210</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16474979</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2005 Jan;165(1):121-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15720627</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Bot. 2003 Jan;91 Spec No:173-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12509338</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2003 May 20;42(19):5764-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12741834</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protoplasma. 2001;218(3-4):125-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11770429</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2002 Jan 11;277(2):871-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11696555</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2009 Jul;37(Web Server issue):W510-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19429685</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1993 Dec 5;234(3):779-815</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8254673</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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<li>France</li>
<li>Suisse</li>
</country>
<region><li>Canton de Zurich</li>
<li>Finlande occidentale</li>
<li>Provence-Alpes-Côte d'Azur</li>
</region>
<settlement><li>Marseille</li>
<li>Turku</li>
<li>Zurich</li>
</settlement>
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<name sortKey="H Ggman, Hely" sort="H Ggman, Hely" uniqKey="H Ggman H" first="Hely" last="H Ggman">Hely H Ggman</name>
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Evolution, three-dimensional model and localization of truncated hemoglobin PttTrHb of hybrid aspen.

Evolution, three-dimensional model and localization of truncated hemoglobin PttTrHb of hybrid aspen.

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