Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling.
Identifieur interne : 002145 ( Main/Exploration ); précédent : 002144; suivant : 002146Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling.
Auteurs : Paula Guzmán [Espagne] ; Victoria Fernández [Espagne] ; María Luisa García [Espagne] ; Mohamed Khayet [Espagne] ; Agustín Fernández [Espagne] ; Luis Gil [Espagne]Source :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2014.
Descripteurs français
- KwdFr :
- Cellulose (composition chimique), Enzymes (composition chimique), Eucalyptus (MeSH), Feuilles de plante (composition chimique), Microscopie électronique à transmission (MeSH), Or (composition chimique), Paroi cellulaire (composition chimique), Pectine (composition chimique), Polyosides (analyse), Polyosides (composition chimique), Populus (MeSH), Pyrus (MeSH), Épiderme végétal (composition chimique).
- MESH :
- analyse : Polyosides.
- composition chimique : Cellulose, Enzymes, Feuilles de plante, Or, Paroi cellulaire, Pectine, Polyosides, Épiderme végétal.
- Eucalyptus, Microscopie électronique à transmission, Populus, Pyrus.
English descriptors
- KwdEn :
- Cell Wall (chemistry), Cellulose (chemistry), Enzymes (chemistry), Eucalyptus (MeSH), Gold (chemistry), Microscopy, Electron, Transmission (MeSH), Pectins (chemistry), Plant Epidermis (chemistry), Plant Leaves (chemistry), Polysaccharides (analysis), Polysaccharides (chemistry), Populus (MeSH), Pyrus (MeSH).
- MESH :
- chemical , analysis : Polysaccharides.
- chemical , chemistry : Cellulose, Enzymes, Gold, Pectins, Polysaccharides.
- chemistry : Cell Wall, Plant Epidermis, Plant Leaves.
- Eucalyptus, Microscopy, Electron, Transmission, Populus, Pyrus.
Abstract
The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus × canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context.
DOI: 10.1016/j.plaphy.2013.12.023
PubMed: 24445334
Affiliations:
Links toward previous steps (curation, corpus...)
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Electronic address: v.fernandez@upm.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Garcia, Maria Luisa" sort="Garcia, Maria Luisa" uniqKey="Garcia M" first="María Luisa" last="García">María Luisa García</name><affiliation wicri:level="2"><nlm:affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Khayet, Mohamed" sort="Khayet, Mohamed" uniqKey="Khayet M" first="Mohamed" last="Khayet">Mohamed Khayet</name><affiliation wicri:level="2"><nlm:affiliation>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Fernandez, Agustin" sort="Fernandez, Agustin" uniqKey="Fernandez A" first="Agustín" last="Fernández">Agustín Fernández</name><affiliation wicri:level="2"><nlm:affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Gil, Luis" sort="Gil, Luis" uniqKey="Gil L" first="Luis" last="Gil">Luis Gil</name><affiliation wicri:level="2"><nlm:affiliation>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Cellulose (chemistry)</term><term>Enzymes (chemistry)</term><term>Eucalyptus (MeSH)</term><term>Gold (chemistry)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Pectins (chemistry)</term><term>Plant Epidermis (chemistry)</term><term>Plant Leaves (chemistry)</term><term>Polysaccharides (analysis)</term><term>Polysaccharides (chemistry)</term><term>Populus (MeSH)</term><term>Pyrus (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellulose (composition chimique)</term><term>Enzymes (composition chimique)</term><term>Eucalyptus (MeSH)</term><term>Feuilles de plante (composition chimique)</term><term>Microscopie électronique à transmission (MeSH)</term><term>Or (composition chimique)</term><term>Paroi cellulaire (composition chimique)</term><term>Pectine (composition chimique)</term><term>Polyosides (analyse)</term><term>Polyosides (composition chimique)</term><term>Populus (MeSH)</term><term>Pyrus (MeSH)</term><term>Épiderme végétal (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Enzymes</term><term>Gold</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Plant Epidermis</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Enzymes</term><term>Feuilles de plante</term><term>Or</term><term>Paroi cellulaire</term><term>Pectine</term><term>Polyosides</term><term>Épiderme végétal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Eucalyptus</term><term>Microscopy, Electron, Transmission</term><term>Populus</term><term>Pyrus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Eucalyptus</term><term>Microscopie électronique à transmission</term><term>Populus</term><term>Pyrus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus × canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24445334</PMID><DateCompleted><Year>2014</Year><Month>10</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>76</Volume><PubDate><Year>2014</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling.</ArticleTitle><Pagination><MedlinePgn>1-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2013.12.023</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(13)00466-X</ELocationID><Abstract><AbstractText>The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus × canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guzmán</LastName><ForeName>Paula</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>Victoria</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. Electronic address: v.fernandez@upm.es.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>García</LastName><ForeName>María Luisa</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khayet</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Applied Physics I, Faculty of Physics, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>Agustín</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Electron Microscopy National Centre, Faculty of Chemistry, University Complutense of Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gil</LastName><ForeName>Luis</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004798">Enzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance UI="D006046">Gold</NameOfSubstance></Chemical><Chemical><RegistryNumber>89NA02M4RX</RegistryNumber><NameOfSubstance UI="D010368">Pectins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004798" MajorTopicYN="N">Enzymes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005052" MajorTopicYN="N">Eucalyptus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006046" MajorTopicYN="N">Gold</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010368" MajorTopicYN="N">Pectins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019441" MajorTopicYN="N">Plant Epidermis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031989" MajorTopicYN="N">Pyrus</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cellulose</Keyword><Keyword MajorTopicYN="N">Cuticle</Keyword><Keyword MajorTopicYN="N">Gold-labelling</Keyword><Keyword MajorTopicYN="N">Leaf</Keyword><Keyword MajorTopicYN="N">Pectins</Keyword><Keyword MajorTopicYN="N">Polysaccharides</Keyword><Keyword MajorTopicYN="N">Transmission electron microscopy</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>12</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>10</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24445334</ArticleId><ArticleId IdType="pii">S0981-9428(13)00466-X</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2013.12.023</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Guzman, Paula" sort="Guzman, Paula" uniqKey="Guzman P" first="Paula" last="Guzmán">Paula Guzmán</name></region><name sortKey="Fernandez, Agustin" sort="Fernandez, Agustin" uniqKey="Fernandez A" first="Agustín" last="Fernández">Agustín Fernández</name><name sortKey="Fernandez, Victoria" sort="Fernandez, Victoria" uniqKey="Fernandez V" first="Victoria" last="Fernández">Victoria Fernández</name><name sortKey="Garcia, Maria Luisa" sort="Garcia, Maria Luisa" uniqKey="Garcia M" first="María Luisa" last="García">María Luisa García</name><name sortKey="Gil, Luis" sort="Gil, Luis" uniqKey="Gil L" first="Luis" last="Gil">Luis Gil</name><name sortKey="Khayet, Mohamed" sort="Khayet, Mohamed" uniqKey="Khayet M" first="Mohamed" last="Khayet">Mohamed Khayet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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