Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.
Identifieur interne : 001951 ( Main/Exploration ); précédent : 001950; suivant : 001952Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.
Auteurs : Guillaume Bourdel [Canada] ; Alice Roy-Bolduc [Canada] ; Marc St-Arnaud [Canada] ; Mohamed Hijri [Canada]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2016.
Abstract
Plant-root inhabiting fungi are a universal phenomenon found in all ecosystems where plants are able to grow, even in harsh environments. Interactions between fungi and plant roots can vary widely from mutualism to parasitism depending on many parameters. The role of fungal endophytes in phytoremediation of polluted sites, and characterization of the endophytic diversity and community assemblages in contaminated areas remain largely unexplored. In this study, we investigated the composition of endophytic fungal communities in the roots of two plant species growing spontaneously in petroleum-contaminated sedimentation basins of a former petro-chemical plant. The three adjacent basins showed a highly heterogeneous pattern of pollutant concentrations. We combined a culture-based isolation approach with the pyrosequencing of fungal ITS ribosomal DNA. We selected two species, Eleocharis erythropoda Steud. and Populus balsamifera L., and sampled three individuals of each species from each of three adjacent basins, each with a different concentration of petroleum hydrocarbons. We found that contamination level significantly shaped endophytic fungal diversity and community composition in E. erythropoda, with only 9.9% of these fungal Operational Taxonomic Units (OTUs) retrieved in all three basins. However, fungal community structure associated with P. balsamifera remained unaffected by the contamination level with 28.2% of fungal OTUs shared among all three basins. This could be explained by the smaller differences of pollutant concentrations in the soil around our set of P. balsamifera sampless compared to that around our set of E. erythropoda samples. Our culture-based approach allowed isolation of 11 and 30 fungal endophytic species from surface-sterilized roots of E. erythropoda and P. balsamifera, respectively. These isolates were ribotyped using ITS, and all were found in pyrosequensing datasets. Our results demonstrate that extreme levels of pollution reduce fungal diversity and shape community composition in E. erythropoda. Our findings shed light on the effect of soil petroleum contamination on fungal endophytic communities and could help to develop strategies for improving phytoremediation using fungal endophytes.
DOI: 10.3389/fmicb.2016.00685
PubMed: 27433155
PubMed Central: PMC4922216
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.</title><author><name sortKey="Bourdel, Guillaume" sort="Bourdel, Guillaume" uniqKey="Bourdel G" first="Guillaume" last="Bourdel">Guillaume Bourdel</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="Roy Bolduc, Alice" sort="Roy Bolduc, Alice" uniqKey="Roy Bolduc A" first="Alice" last="Roy-Bolduc">Alice Roy-Bolduc</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="St Arnaud, Marc" sort="St Arnaud, Marc" uniqKey="St Arnaud M" first="Marc" last="St-Arnaud">Marc St-Arnaud</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="Hijri, Mohamed" sort="Hijri, Mohamed" uniqKey="Hijri M" first="Mohamed" last="Hijri">Mohamed Hijri</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27433155</idno><idno type="pmid">27433155</idno><idno type="doi">10.3389/fmicb.2016.00685</idno><idno type="pmc">PMC4922216</idno><idno type="wicri:Area/Main/Corpus">001703</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001703</idno><idno type="wicri:Area/Main/Curation">001703</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001703</idno><idno type="wicri:Area/Main/Exploration">001703</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.</title><author><name sortKey="Bourdel, Guillaume" sort="Bourdel, Guillaume" uniqKey="Bourdel G" first="Guillaume" last="Bourdel">Guillaume Bourdel</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="Roy Bolduc, Alice" sort="Roy Bolduc, Alice" uniqKey="Roy Bolduc A" first="Alice" last="Roy-Bolduc">Alice Roy-Bolduc</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="St Arnaud, Marc" sort="St Arnaud, Marc" uniqKey="St Arnaud M" first="Marc" last="St-Arnaud">Marc St-Arnaud</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author><author><name sortKey="Hijri, Mohamed" sort="Hijri, Mohamed" uniqKey="Hijri M" first="Mohamed" last="Hijri">Mohamed Hijri</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC</wicri:regionArea><wicri:noRegion>Montréal QC</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant-root inhabiting fungi are a universal phenomenon found in all ecosystems where plants are able to grow, even in harsh environments. Interactions between fungi and plant roots can vary widely from mutualism to parasitism depending on many parameters. The role of fungal endophytes in phytoremediation of polluted sites, and characterization of the endophytic diversity and community assemblages in contaminated areas remain largely unexplored. In this study, we investigated the composition of endophytic fungal communities in the roots of two plant species growing spontaneously in petroleum-contaminated sedimentation basins of a former petro-chemical plant. The three adjacent basins showed a highly heterogeneous pattern of pollutant concentrations. We combined a culture-based isolation approach with the pyrosequencing of fungal ITS ribosomal DNA. We selected two species, Eleocharis erythropoda Steud. and Populus balsamifera L., and sampled three individuals of each species from each of three adjacent basins, each with a different concentration of petroleum hydrocarbons. We found that contamination level significantly shaped endophytic fungal diversity and community composition in E. erythropoda, with only 9.9% of these fungal Operational Taxonomic Units (OTUs) retrieved in all three basins. However, fungal community structure associated with P. balsamifera remained unaffected by the contamination level with 28.2% of fungal OTUs shared among all three basins. This could be explained by the smaller differences of pollutant concentrations in the soil around our set of P. balsamifera sampless compared to that around our set of E. erythropoda samples. Our culture-based approach allowed isolation of 11 and 30 fungal endophytic species from surface-sterilized roots of E. erythropoda and P. balsamifera, respectively. These isolates were ribotyped using ITS, and all were found in pyrosequensing datasets. Our results demonstrate that extreme levels of pollution reduce fungal diversity and shape community composition in E. erythropoda. Our findings shed light on the effect of soil petroleum contamination on fungal endophytic communities and could help to develop strategies for improving phytoremediation using fungal endophytes. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27433155</PMID><DateCompleted><Year>2016</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.</ArticleTitle><Pagination><MedlinePgn>685</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2016.00685</ELocationID><Abstract><AbstractText>Plant-root inhabiting fungi are a universal phenomenon found in all ecosystems where plants are able to grow, even in harsh environments. Interactions between fungi and plant roots can vary widely from mutualism to parasitism depending on many parameters. The role of fungal endophytes in phytoremediation of polluted sites, and characterization of the endophytic diversity and community assemblages in contaminated areas remain largely unexplored. In this study, we investigated the composition of endophytic fungal communities in the roots of two plant species growing spontaneously in petroleum-contaminated sedimentation basins of a former petro-chemical plant. The three adjacent basins showed a highly heterogeneous pattern of pollutant concentrations. We combined a culture-based isolation approach with the pyrosequencing of fungal ITS ribosomal DNA. We selected two species, Eleocharis erythropoda Steud. and Populus balsamifera L., and sampled three individuals of each species from each of three adjacent basins, each with a different concentration of petroleum hydrocarbons. We found that contamination level significantly shaped endophytic fungal diversity and community composition in E. erythropoda, with only 9.9% of these fungal Operational Taxonomic Units (OTUs) retrieved in all three basins. However, fungal community structure associated with P. balsamifera remained unaffected by the contamination level with 28.2% of fungal OTUs shared among all three basins. This could be explained by the smaller differences of pollutant concentrations in the soil around our set of P. balsamifera sampless compared to that around our set of E. erythropoda samples. Our culture-based approach allowed isolation of 11 and 30 fungal endophytic species from surface-sterilized roots of E. erythropoda and P. balsamifera, respectively. These isolates were ribotyped using ITS, and all were found in pyrosequensing datasets. Our results demonstrate that extreme levels of pollution reduce fungal diversity and shape community composition in E. erythropoda. Our findings shed light on the effect of soil petroleum contamination on fungal endophytic communities and could help to develop strategies for improving phytoremediation using fungal endophytes. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bourdel</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roy-Bolduc</LastName><ForeName>Alice</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>St-Arnaud</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hijri</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal QC, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Eleocharis erythropoda</Keyword><Keyword MajorTopicYN="N">Populus balsamifera L.</Keyword><Keyword MajorTopicYN="N">endophytes</Keyword><Keyword MajorTopicYN="N">fungi</Keyword><Keyword MajorTopicYN="N">petroleum-hydrocarbon contamination</Keyword><Keyword MajorTopicYN="N">pyrosequencing</Keyword><Keyword MajorTopicYN="N">ribosomal DNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27433155</ArticleId><ArticleId IdType="doi">10.3389/fmicb.2016.00685</ArticleId><ArticleId IdType="pmc">PMC4922216</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>New Phytol. 2014 Jan;201(2):599-609</Citation><ArticleIdList><ArticleId IdType="pubmed">24117518</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecology. 2012 Dec;93(12):2533-47</Citation><ArticleIdList><ArticleId IdType="pubmed">23431585</ArticleId></ArticleIdList></Reference><Reference><Citation>Chemosphere. 2010 Nov;81(9):1084-90</Citation><ArticleIdList><ArticleId IdType="pubmed">20961596</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2011;49:291-315</Citation><ArticleIdList><ArticleId IdType="pubmed">19400639</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:643-668</Citation><ArticleIdList><ArticleId IdType="pubmed">15012249</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2010 Oct;188(1):291-301</Citation><ArticleIdList><ArticleId IdType="pubmed">20636324</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2006;57:233-66</Citation><ArticleIdList><ArticleId IdType="pubmed">16669762</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2006 Aug;9(4):358-63</Citation><ArticleIdList><ArticleId IdType="pubmed">16713330</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2011 Nov 15;2:217</Citation><ArticleIdList><ArticleId IdType="pubmed">22131985</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Pollut Res Int. 2014 Jun;21(12):7538-47</Citation><ArticleIdList><ArticleId IdType="pubmed">24595752</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2004;55:315-40</Citation><ArticleIdList><ArticleId IdType="pubmed">15377223</ArticleId></ArticleIdList></Reference><Reference><Citation>N Biotechnol. 2013 Sep 25;30(6):780-7</Citation><ArticleIdList><ArticleId IdType="pubmed">23876814</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2006 May;223(6):1115-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16555102</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1999 Jun;65(6):2741-4</Citation><ArticleIdList><ArticleId IdType="pubmed">10347070</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2006 Aug;9(4):364-70</Citation><ArticleIdList><ArticleId IdType="pubmed">16713732</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Toxicol Chem. 2008 May;27(5):1039-46</Citation><ArticleIdList><ArticleId IdType="pubmed">18419193</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2005;56:15-39</Citation><ArticleIdList><ArticleId IdType="pubmed">15862088</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Prod Rep. 2001 Aug;18(4):448-59</Citation><ArticleIdList><ArticleId IdType="pubmed">11548053</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2003 Jul 1;4(4):225-36</Citation><ArticleIdList><ArticleId IdType="pubmed">20569383</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2010 May;7(5):335-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20383131</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2009;182(2):314-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19236579</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2011 Jan;14(1):19-28</Citation><ArticleIdList><ArticleId IdType="pubmed">21070562</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biotechnol. 2013 Jul;54(3):900-12</Citation><ArticleIdList><ArticleId IdType="pubmed">23307295</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Jun 08;10(6):e0128272</Citation><ArticleIdList><ArticleId IdType="pubmed">26053848</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2012 Aug 2;488(7409):91-5</Citation><ArticleIdList><ArticleId IdType="pubmed">22859207</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2010 Oct 1;26(19):2460-1</Citation><ArticleIdList><ArticleId IdType="pubmed">20709691</ArticleId></ArticleIdList></Reference><Reference><Citation>ISME J. 2014 Feb;8(2):331-43</Citation><ArticleIdList><ArticleId IdType="pubmed">23985744</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Pollut Res Int. 2005;12(1):34-48</Citation><ArticleIdList><ArticleId IdType="pubmed">15768739</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2009 Dec;75(23):7537-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19801464</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2013 Nov;22(21):5271-7</Citation><ArticleIdList><ArticleId IdType="pubmed">24112409</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Ecol. 2007 Nov;62(2):142-60</Citation><ArticleIdList><ArticleId IdType="pubmed">17892477</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 1993 Apr;2(2):113-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8180733</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1977 Nov;34(5):576-81</Citation><ArticleIdList><ArticleId IdType="pubmed">931377</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2005 Jun;109(Pt 6):661-86</Citation><ArticleIdList><ArticleId IdType="pubmed">16080390</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2011 Aug;20(16):3469-83</Citation><ArticleIdList><ArticleId IdType="pubmed">21668808</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Bourdel, Guillaume" sort="Bourdel, Guillaume" uniqKey="Bourdel G" first="Guillaume" last="Bourdel">Guillaume Bourdel</name></noRegion><name sortKey="Hijri, Mohamed" sort="Hijri, Mohamed" uniqKey="Hijri M" first="Mohamed" last="Hijri">Mohamed Hijri</name><name sortKey="Roy Bolduc, Alice" sort="Roy Bolduc, Alice" uniqKey="Roy Bolduc A" first="Alice" last="Roy-Bolduc">Alice Roy-Bolduc</name><name sortKey="St Arnaud, Marc" sort="St Arnaud, Marc" uniqKey="St Arnaud M" first="Marc" last="St-Arnaud">Marc St-Arnaud</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001951 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001951 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27433155
|texte= Concentration of Petroleum-Hydrocarbon Contamination Shapes Fungal Endophytic Community Structure in Plant Roots.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27433155" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |