Integration of genetic, genomic and transcriptomic information identifies putative regulators of adventitious root formation in Populus.
Identifieur interne : 001809 ( Main/Exploration ); précédent : 001808; suivant : 001810Integration of genetic, genomic and transcriptomic information identifies putative regulators of adventitious root formation in Populus.
Auteurs : Cintia L. Ribeiro [États-Unis] ; Cynthia M. Silva [États-Unis] ; Derek R. Drost [États-Unis] ; Evandro Novaes [États-Unis, Brésil] ; Carolina R D B. Novaes [États-Unis, Brésil] ; Christopher Dervinis [États-Unis] ; Matias Kirst [États-Unis]Source :
- BMC plant biology [ 1471-2229 ] ; 2016.
Descripteurs français
- KwdFr :
- Allèles (MeSH), Analyse de profil d'expression de gènes (MeSH), Facteurs de transcription (métabolisme), Gènes de plante (MeSH), Génome végétal (MeSH), Locus de caractère quantitatif (MeSH), Populus (croissance et développement), Populus (génétique), Protéines végétales (métabolisme), Racines de plante (croissance et développement), Racines de plante (génétique), Sites de fixation (MeSH).
- MESH :
- croissance et développement : Populus, Racines de plante.
- génétique : Populus, Racines de plante.
- métabolisme : Facteurs de transcription, Protéines végétales.
- Allèles, Analyse de profil d'expression de gènes, Gènes de plante, Génome végétal, Locus de caractère quantitatif, Sites de fixation.
English descriptors
- KwdEn :
- Alleles (MeSH), Binding Sites (MeSH), Gene Expression Profiling (MeSH), Genes, Plant (MeSH), Genome, Plant (MeSH), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (growth & development), Populus (genetics), Populus (growth & development), Quantitative Trait Loci (MeSH), Transcription Factors (metabolism).
- MESH :
- chemical , metabolism : Plant Proteins, Transcription Factors.
- genetics : Plant Roots, Populus.
- growth & development : Plant Roots, Populus.
- Alleles, Binding Sites, Gene Expression Profiling, Genes, Plant, Genome, Plant, Quantitative Trait Loci.
Abstract
BACKGROUND
Adventitious roots (AR) develop from tissues other than the primary root, in a process physiologically regulated by phytohormones. Adventitious roots provide structural support and contribute to water and nutrient absorption, and are critical for commercial vegetative propagation of several crops. Here we quantified the number of AR, root architectural traits and root biomass in cuttings from a pseudo-backcross population of Populus deltoides and Populus trichocarpa. Quantitative trait loci (QTL) mapping and whole-transcriptome analysis of individuals with alternative QTL alleles for AR number were used to identify putative regulators of AR development.
RESULTS
Parental individuals and progeny showed extensive segregation for AR developmental traits. Quantitative trait loci for number of AR mapped consistently in the same interval of linkage group (LG) II and LG XIV, explaining 7-10 % of the phenotypic variation. A time series transcriptome analysis identified 26,121 genes differentially expressed during AR development, particularly during the first 24 h after cuttings were harvested. Of those, 1929 genes were differentially regulated between individuals carrying alternative alleles for the two QTL for number of AR, in one or more time point. Eighty-one of these genes were physically located within the QTL intervals for number of AR, including putative homologs of the Arabidopsis genes SUPERROOT2 (SUR2) and TRYPTOPHAN SYNTHASE ALPHA CHAIN (TSA1), both of which are involved in the auxin indole-3-acetic acid (IAA) biosynthesis pathway.
CONCLUSIONS
This study suggests the involvement of two genes of the tryptophan-dependent auxin biosynthesis pathway, SUR2 and TSA1, in the regulation of a critical trait for the clonal propagation of woody species. A possible model for this regulation is that poplar individuals that have poor AR formation synthesize auxin indole-3-acetic acid (IAA) primarily through the tryptophan (Trp) pathway. Much of the Trp pathway flux appears to be directed to the synthesis of indole glucosinolates (IG), as suggested by the over-expression of SUR2. Individuals that are efficient in AR formation may utilize alternative (non-Trp) pathways to synthesize IAA, based on the observation that they down-regulate the expression of TSA1, one of the critical steps in the synthesis of tryptophan.
DOI: 10.1186/s12870-016-0753-0
PubMed: 26983547
PubMed Central: PMC4793515
Affiliations:
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Box 110690, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Seminis, Inc., 37437 State Highway 16, Woodland, CA, 95695, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Present Address: Seminis, Inc., 37437 State Highway 16, Woodland, CA, 95695</wicri:regionArea><wicri:noRegion>95695</wicri:noRegion></affiliation></author><author><name sortKey="Novaes, Evandro" sort="Novaes, Evandro" uniqKey="Novaes E" first="Evandro" last="Novaes">Evandro Novaes</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970</wicri:regionArea><wicri:noRegion>74001-970</wicri:noRegion></affiliation></author><author><name sortKey="Novaes, Carolina R D B" sort="Novaes, Carolina R D B" uniqKey="Novaes C" first="Carolina R D B" last="Novaes">Carolina R D B. Novaes</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970</wicri:regionArea><wicri:noRegion>74001-970</wicri:noRegion></affiliation></author><author><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author><author><name sortKey="Kirst, Matias" sort="Kirst, Matias" uniqKey="Kirst M" first="Matias" last="Kirst">Matias Kirst</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>University of Florida Genetics Institute, University of Florida, P.O. Box 103610, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Florida Genetics Institute, University of Florida, P.O. Box 103610, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26983547</idno><idno type="pmid">26983547</idno><idno type="doi">10.1186/s12870-016-0753-0</idno><idno type="pmc">PMC4793515</idno><idno type="wicri:Area/Main/Corpus">001866</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001866</idno><idno type="wicri:Area/Main/Curation">001866</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001866</idno><idno type="wicri:Area/Main/Exploration">001866</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Integration of genetic, genomic and transcriptomic information identifies putative regulators of adventitious root formation in Populus.</title><author><name sortKey="Ribeiro, Cintia L" sort="Ribeiro, Cintia L" uniqKey="Ribeiro C" first="Cintia L" last="Ribeiro">Cintia L. Ribeiro</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Monsanto Company, 700 Chesterfield Pkwy W, Chesterfield, MO, 63017, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Present Address: Monsanto Company, 700 Chesterfield Pkwy W, Chesterfield, MO, 63017</wicri:regionArea><wicri:noRegion>63017</wicri:noRegion></affiliation></author><author><name sortKey="Silva, Cynthia M" sort="Silva, Cynthia M" uniqKey="Silva C" first="Cynthia M" last="Silva">Cynthia M. Silva</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author><author><name sortKey="Drost, Derek R" sort="Drost, Derek R" uniqKey="Drost D" first="Derek R" last="Drost">Derek R. Drost</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Seminis, Inc., 37437 State Highway 16, Woodland, CA, 95695, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Present Address: Seminis, Inc., 37437 State Highway 16, Woodland, CA, 95695</wicri:regionArea><wicri:noRegion>95695</wicri:noRegion></affiliation></author><author><name sortKey="Novaes, Evandro" sort="Novaes, Evandro" uniqKey="Novaes E" first="Evandro" last="Novaes">Evandro Novaes</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970</wicri:regionArea><wicri:noRegion>74001-970</wicri:noRegion></affiliation></author><author><name sortKey="Novaes, Carolina R D B" sort="Novaes, Carolina R D B" uniqKey="Novaes C" first="Carolina R D B" last="Novaes">Carolina R D B. Novaes</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970</wicri:regionArea><wicri:noRegion>74001-970</wicri:noRegion></affiliation></author><author><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author><author><name sortKey="Kirst, Matias" sort="Kirst, Matias" uniqKey="Kirst M" first="Matias" last="Kirst">Matias Kirst</name><affiliation wicri:level="1"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>University of Florida Genetics Institute, University of Florida, P.O. Box 103610, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Florida Genetics Institute, University of Florida, P.O. Box 103610, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles (MeSH)</term><term>Binding Sites (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (genetics)</term><term>Plant Roots (growth & development)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Quantitative Trait Loci (MeSH)</term><term>Transcription Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allèles (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Facteurs de transcription (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Génome végétal (MeSH)</term><term>Locus de caractère quantitatif (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (génétique)</term><term>Sites de fixation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Binding Sites</term><term>Gene Expression Profiling</term><term>Genes, Plant</term><term>Genome, Plant</term><term>Quantitative Trait Loci</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Analyse de profil d'expression de gènes</term><term>Gènes de plante</term><term>Génome végétal</term><term>Locus de caractère quantitatif</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Adventitious roots (AR) develop from tissues other than the primary root, in a process physiologically regulated by phytohormones. Adventitious roots provide structural support and contribute to water and nutrient absorption, and are critical for commercial vegetative propagation of several crops. Here we quantified the number of AR, root architectural traits and root biomass in cuttings from a pseudo-backcross population of Populus deltoides and Populus trichocarpa. Quantitative trait loci (QTL) mapping and whole-transcriptome analysis of individuals with alternative QTL alleles for AR number were used to identify putative regulators of AR development.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Parental individuals and progeny showed extensive segregation for AR developmental traits. Quantitative trait loci for number of AR mapped consistently in the same interval of linkage group (LG) II and LG XIV, explaining 7-10 % of the phenotypic variation. A time series transcriptome analysis identified 26,121 genes differentially expressed during AR development, particularly during the first 24 h after cuttings were harvested. Of those, 1929 genes were differentially regulated between individuals carrying alternative alleles for the two QTL for number of AR, in one or more time point. Eighty-one of these genes were physically located within the QTL intervals for number of AR, including putative homologs of the Arabidopsis genes SUPERROOT2 (SUR2) and TRYPTOPHAN SYNTHASE ALPHA CHAIN (TSA1), both of which are involved in the auxin indole-3-acetic acid (IAA) biosynthesis pathway.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This study suggests the involvement of two genes of the tryptophan-dependent auxin biosynthesis pathway, SUR2 and TSA1, in the regulation of a critical trait for the clonal propagation of woody species. A possible model for this regulation is that poplar individuals that have poor AR formation synthesize auxin indole-3-acetic acid (IAA) primarily through the tryptophan (Trp) pathway. Much of the Trp pathway flux appears to be directed to the synthesis of indole glucosinolates (IG), as suggested by the over-expression of SUR2. Individuals that are efficient in AR formation may utilize alternative (non-Trp) pathways to synthesize IAA, based on the observation that they down-regulate the expression of TSA1, one of the critical steps in the synthesis of tryptophan.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26983547</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><PubDate><Year>2016</Year><Month>Mar</Month><Day>16</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Integration of genetic, genomic and transcriptomic information identifies putative regulators of adventitious root formation in Populus.</ArticleTitle><Pagination><MedlinePgn>66</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-016-0753-0</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Adventitious roots (AR) develop from tissues other than the primary root, in a process physiologically regulated by phytohormones. Adventitious roots provide structural support and contribute to water and nutrient absorption, and are critical for commercial vegetative propagation of several crops. Here we quantified the number of AR, root architectural traits and root biomass in cuttings from a pseudo-backcross population of Populus deltoides and Populus trichocarpa. Quantitative trait loci (QTL) mapping and whole-transcriptome analysis of individuals with alternative QTL alleles for AR number were used to identify putative regulators of AR development.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Parental individuals and progeny showed extensive segregation for AR developmental traits. Quantitative trait loci for number of AR mapped consistently in the same interval of linkage group (LG) II and LG XIV, explaining 7-10 % of the phenotypic variation. A time series transcriptome analysis identified 26,121 genes differentially expressed during AR development, particularly during the first 24 h after cuttings were harvested. Of those, 1929 genes were differentially regulated between individuals carrying alternative alleles for the two QTL for number of AR, in one or more time point. Eighty-one of these genes were physically located within the QTL intervals for number of AR, including putative homologs of the Arabidopsis genes SUPERROOT2 (SUR2) and TRYPTOPHAN SYNTHASE ALPHA CHAIN (TSA1), both of which are involved in the auxin indole-3-acetic acid (IAA) biosynthesis pathway.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This study suggests the involvement of two genes of the tryptophan-dependent auxin biosynthesis pathway, SUR2 and TSA1, in the regulation of a critical trait for the clonal propagation of woody species. A possible model for this regulation is that poplar individuals that have poor AR formation synthesize auxin indole-3-acetic acid (IAA) primarily through the tryptophan (Trp) pathway. Much of the Trp pathway flux appears to be directed to the synthesis of indole glucosinolates (IG), as suggested by the over-expression of SUR2. Individuals that are efficient in AR formation may utilize alternative (non-Trp) pathways to synthesize IAA, based on the observation that they down-regulate the expression of TSA1, one of the critical steps in the synthesis of tryptophan.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ribeiro</LastName><ForeName>Cintia L</ForeName><Initials>CL</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Present Address: Monsanto Company, 700 Chesterfield Pkwy W, Chesterfield, MO, 63017, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Cynthia M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Drost</LastName><ForeName>Derek R</ForeName><Initials>DR</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Present Address: Seminis, Inc., 37437 State Highway 16, Woodland, CA, 95695, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Novaes</LastName><ForeName>Evandro</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Novaes</LastName><ForeName>Carolina R D B</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Present Address: Universidade Federal de Goiás, Av. Esperança s/n°, Goiânia, GO, 74001-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dervinis</LastName><ForeName>Christopher</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kirst</LastName><ForeName>Matias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Molecular and Cellular Biology Graduate Program, University of Florida, P.O. Box 110690, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Florida Genetics Institute, University of Florida, P.O. Box 103610, Gainesville, FL, 32611, USA. mkirst@ufl.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Plant Biol</MedlineTA><NlmUniqueID>100967807</NlmUniqueID><ISSNLinking>1471-2229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040641" MajorTopicYN="N">Quantitative Trait Loci</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Adventitious root</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">QTL</Keyword><Keyword MajorTopicYN="N">SUR2</Keyword><Keyword MajorTopicYN="N">Vegetative propagation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate 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Ribeiro</name></noRegion><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name><name sortKey="Drost, Derek R" sort="Drost, Derek R" uniqKey="Drost D" first="Derek R" last="Drost">Derek R. Drost</name><name sortKey="Drost, Derek R" sort="Drost, Derek R" uniqKey="Drost D" first="Derek R" last="Drost">Derek R. Drost</name><name sortKey="Drost, Derek R" sort="Drost, Derek R" uniqKey="Drost D" first="Derek R" last="Drost">Derek R. Drost</name><name sortKey="Kirst, Matias" sort="Kirst, Matias" uniqKey="Kirst M" first="Matias" last="Kirst">Matias Kirst</name><name sortKey="Kirst, Matias" sort="Kirst, Matias" uniqKey="Kirst M" first="Matias" last="Kirst">Matias Kirst</name><name sortKey="Kirst, Matias" sort="Kirst, Matias" uniqKey="Kirst M" first="Matias" last="Kirst">Matias Kirst</name><name sortKey="Novaes, Carolina R D B" sort="Novaes, Carolina R D B" uniqKey="Novaes C" first="Carolina R D B" last="Novaes">Carolina R D B. Novaes</name><name sortKey="Novaes, Evandro" sort="Novaes, Evandro" uniqKey="Novaes E" first="Evandro" last="Novaes">Evandro Novaes</name><name sortKey="Ribeiro, Cintia L" sort="Ribeiro, Cintia L" uniqKey="Ribeiro C" first="Cintia L" last="Ribeiro">Cintia L. Ribeiro</name><name sortKey="Ribeiro, Cintia L" sort="Ribeiro, Cintia L" uniqKey="Ribeiro C" first="Cintia L" last="Ribeiro">Cintia L. Ribeiro</name><name sortKey="Silva, Cynthia M" sort="Silva, Cynthia M" uniqKey="Silva C" first="Cynthia M" last="Silva">Cynthia M. Silva</name></country><country name="Brésil"><noRegion><name sortKey="Novaes, Evandro" sort="Novaes, Evandro" uniqKey="Novaes E" first="Evandro" last="Novaes">Evandro Novaes</name></noRegion><name sortKey="Novaes, Carolina R D B" sort="Novaes, Carolina R D B" uniqKey="Novaes C" first="Carolina R D B" last="Novaes">Carolina R D B. Novaes</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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