Low temperatures are required to induce the development of fertile flowers in transgenic male and female early flowering poplar (Populus tremula L.).
Identifieur interne : 001782 ( Main/Exploration ); précédent : 001781; suivant : 001783Low temperatures are required to induce the development of fertile flowers in transgenic male and female early flowering poplar (Populus tremula L.).
Auteurs : Hans Hoenicka [Allemagne] ; Denise Lehnhardt [Allemagne] ; Valentina Briones [Argentine] ; Ove Nilsson [Suède] ; Matthias Fladung [Allemagne]Source :
- Tree physiology [ 1758-4469 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Fleurs, Populus, Végétaux génétiquement modifiés.
- génétique : Fleurs, Populus, Végétaux génétiquement modifiés.
- Basse température.
English descriptors
- KwdEn :
- MESH :
- genetics : Flowers, Plants, Genetically Modified, Populus.
- growth & development : Flowers, Plants, Genetically Modified, Populus.
- Cold Temperature.
Abstract
Until now, artificial early flowering poplar systems have mostly led to the development of sterile flowers. In this study, several strategies aimed at inducting fertile flowers in pHSP::AtFT transgenic poplar were evaluated, in particular the influence of temperature and photoperiod. Our results provide evidence that temperature, and not photoperiod, is the key factor required for the development of fertile flowers in early flowering poplar. Fertile flowers were only obtained when a cold treatment phase of several weeks was used after the heat treatment phase. Heat treatments induced AtFT gene activity through activation of the heat-shock promoter (pHSP). Photoperiod did not show a similar influence on flower fertility as pollen grains were obtained under both long- and short-day conditions. Fertility was confirmed in flowers of both male and female plants. For the first time, crosses were successfully performed with transgenic female early flowering poplar. All mature flowers obtained after 8 weeks of inductive treatments were fertile. Gene expression studies also confirmed that cold temperatures influenced expression of poplar genes homologous to 'pollen development genes' from Arabidopsis thaliana (L.) Heynh. Homology and expression patterns suggested a role for PtTDF1, PtBAM1, PtSERK1/2 and PtMS1 on anther and pollen development in poplar flowers. The system developed in this study allows a fast and very reliable induction of fertile poplar flowers in a very short period of time. The non-reproductive phase, usually 7-10 years, can now be shortened to 6-10 months, and fertile flowers can be obtained independently of the season. This system is a reliable tool for breeding purposes (high-speed breeding technology), genomics and biosafety research.
DOI: 10.1093/treephys/tpw015
PubMed: 27052434
PubMed Central: PMC4886290
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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D" first="Denise" last="Lehnhardt">Denise Lehnhardt</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf</wicri:regionArea><wicri:noRegion>22927 Grosshansdorf</wicri:noRegion><wicri:noRegion>22927 Grosshansdorf</wicri:noRegion><wicri:noRegion>D-22927 Grosshansdorf</wicri:noRegion></affiliation></author><author><name sortKey="Briones, Valentina" sort="Briones, Valentina" uniqKey="Briones V" first="Valentina" last="Briones">Valentina Briones</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany Universidad Nacional de la Plata, 1900 La Plata, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany Universidad Nacional de la Plata, 1900 La Plata</wicri:regionArea><wicri:noRegion>1900 La Plata</wicri:noRegion></affiliation></author><author><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name><affiliation wicri:level="1"><nlm:affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90183 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90183 Umeå</wicri:regionArea><wicri:noRegion>S-90183 Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany hans.hoenicka@ti.bund.de matthias.fladung@ti.bund.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf</wicri:regionArea><wicri:noRegion>22927 Grosshansdorf</wicri:noRegion><wicri:noRegion>22927 Grosshansdorf</wicri:noRegion><wicri:noRegion>D-22927 Grosshansdorf</wicri:noRegion></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cold Temperature (MeSH)</term><term>Flowers (genetics)</term><term>Flowers (growth & development)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basse température (MeSH)</term><term>Fleurs (croissance et développement)</term><term>Fleurs (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Fleurs</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flowers</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Flowers</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fleurs</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cold Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basse température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Until now, artificial early flowering poplar systems have mostly led to the development of sterile flowers. In this study, several strategies aimed at inducting fertile flowers in pHSP::AtFT transgenic poplar were evaluated, in particular the influence of temperature and photoperiod. Our results provide evidence that temperature, and not photoperiod, is the key factor required for the development of fertile flowers in early flowering poplar. Fertile flowers were only obtained when a cold treatment phase of several weeks was used after the heat treatment phase. Heat treatments induced AtFT gene activity through activation of the heat-shock promoter (pHSP). Photoperiod did not show a similar influence on flower fertility as pollen grains were obtained under both long- and short-day conditions. Fertility was confirmed in flowers of both male and female plants. For the first time, crosses were successfully performed with transgenic female early flowering poplar. All mature flowers obtained after 8 weeks of inductive treatments were fertile. Gene expression studies also confirmed that cold temperatures influenced expression of poplar genes homologous to 'pollen development genes' from Arabidopsis thaliana (L.) Heynh. Homology and expression patterns suggested a role for PtTDF1, PtBAM1, PtSERK1/2 and PtMS1 on anther and pollen development in poplar flowers. The system developed in this study allows a fast and very reliable induction of fertile poplar flowers in a very short period of time. The non-reproductive phase, usually 7-10 years, can now be shortened to 6-10 months, and fertile flowers can be obtained independently of the season. This system is a reliable tool for breeding purposes (high-speed breeding technology), genomics and biosafety research.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27052434</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>20</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>36</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>05</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Low temperatures are required to induce the development of fertile flowers in transgenic male and female early flowering poplar (Populus tremula L.).</ArticleTitle><Pagination><MedlinePgn>667-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpw015</ELocationID><Abstract><AbstractText>Until now, artificial early flowering poplar systems have mostly led to the development of sterile flowers. In this study, several strategies aimed at inducting fertile flowers in pHSP::AtFT transgenic poplar were evaluated, in particular the influence of temperature and photoperiod. Our results provide evidence that temperature, and not photoperiod, is the key factor required for the development of fertile flowers in early flowering poplar. Fertile flowers were only obtained when a cold treatment phase of several weeks was used after the heat treatment phase. Heat treatments induced AtFT gene activity through activation of the heat-shock promoter (pHSP). Photoperiod did not show a similar influence on flower fertility as pollen grains were obtained under both long- and short-day conditions. Fertility was confirmed in flowers of both male and female plants. For the first time, crosses were successfully performed with transgenic female early flowering poplar. All mature flowers obtained after 8 weeks of inductive treatments were fertile. Gene expression studies also confirmed that cold temperatures influenced expression of poplar genes homologous to 'pollen development genes' from Arabidopsis thaliana (L.) Heynh. Homology and expression patterns suggested a role for PtTDF1, PtBAM1, PtSERK1/2 and PtMS1 on anther and pollen development in poplar flowers. The system developed in this study allows a fast and very reliable induction of fertile poplar flowers in a very short period of time. The non-reproductive phase, usually 7-10 years, can now be shortened to 6-10 months, and fertile flowers can be obtained independently of the season. This system is a reliable tool for breeding purposes (high-speed breeding technology), genomics and biosafety research.</AbstractText><CopyrightInformation>© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hoenicka</LastName><ForeName>Hans</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany hans.hoenicka@ti.bund.de matthias.fladung@ti.bund.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lehnhardt</LastName><ForeName>Denise</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Briones</LastName><ForeName>Valentina</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany Universidad Nacional de la Plata, 1900 La Plata, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nilsson</LastName><ForeName>Ove</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90183 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fladung</LastName><ForeName>Matthias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, D-22927 Grosshansdorf, Germany hans.hoenicka@ti.bund.de matthias.fladung@ti.bund.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003080" MajorTopicYN="N">Cold Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">cold treatment</Keyword><Keyword MajorTopicYN="Y">fertility</Keyword><Keyword MajorTopicYN="Y">ovule development</Keyword><Keyword MajorTopicYN="Y">pollen development</Keyword><Keyword MajorTopicYN="Y">poplar breeding</Keyword><Keyword MajorTopicYN="Y">sterility</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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name="Argentine"><noRegion><name sortKey="Briones, Valentina" sort="Briones, Valentina" uniqKey="Briones V" first="Valentina" last="Briones">Valentina Briones</name></noRegion></country><country name="Suède"><noRegion><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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