Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.
Identifieur interne : 001062 ( Main/Exploration ); précédent : 001061; suivant : 001063Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.
Auteurs : Raid Al Akeel [Arabie saoudite] ; Ayesha Mateen [Arabie saoudite] ; Rabbani Syed [Arabie saoudite] ; Mohammed S. Alqahtani [Arabie saoudite] ; Ali S. Alqahtani [Arabie saoudite]Source :
- Microbial pathogenesis [ 1096-1208 ] ; 2018.
Descripteurs français
- KwdFr :
- Alanine (composition chimique), Antienzymes (pharmacologie), Conformation des protéines (MeSH), Histidine kinase (antagonistes et inhibiteurs), Multirésistance bactérienne aux médicaments (MeSH), Peptides antimicrobiens cationiques (pharmacologie), Populus (composition chimique), Protéines bactériennes (antagonistes et inhibiteurs), Protéomique (MeSH), Régulation de l'expression des gènes bactériens (MeSH), Simulation de docking moléculaire (MeSH), Spectrométrie de masse en tandem (MeSH), Staphylococcus aureus (effets des médicaments et des substances chimiques), Tests de sensibilité microbienne (MeSH).
- MESH :
- antagonistes et inhibiteurs : Histidine kinase, Protéines bactériennes.
- composition chimique : Alanine, Populus.
- effets des médicaments et des substances chimiques : Staphylococcus aureus.
- pharmacologie : Antienzymes, Peptides antimicrobiens cationiques.
- Conformation des protéines, Multirésistance bactérienne aux médicaments, Protéomique, Régulation de l'expression des gènes bactériens, Simulation de docking moléculaire, Spectrométrie de masse en tandem, Tests de sensibilité microbienne.
English descriptors
- KwdEn :
- Alanine (chemistry), Antimicrobial Cationic Peptides (pharmacology), Bacterial Proteins (antagonists & inhibitors), Drug Resistance, Multiple, Bacterial (MeSH), Enzyme Inhibitors (pharmacology), Gene Expression Regulation, Bacterial (MeSH), Histidine Kinase (antagonists & inhibitors), Microbial Sensitivity Tests (MeSH), Molecular Docking Simulation (MeSH), Populus (chemistry), Protein Conformation (MeSH), Proteomics (MeSH), Staphylococcus aureus (drug effects), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , antagonists & inhibitors : Bacterial Proteins, Histidine Kinase.
- chemical , chemistry : Alanine.
- chemical , pharmacology : Antimicrobial Cationic Peptides, Enzyme Inhibitors.
- chemistry : Populus.
- drug effects : Staphylococcus aureus.
- Drug Resistance, Multiple, Bacterial, Gene Expression Regulation, Bacterial, Microbial Sensitivity Tests, Molecular Docking Simulation, Protein Conformation, Proteomics, Tandem Mass Spectrometry.
Abstract
BACKGROUND
Due to growing concern towards microbial resistance, ongoing search for developing novel bioactive compounds such as peptides is on rise. The aim of this study was to evaluate antimicrobial effect of Populus trichocarpa extract, chemically identify the active peptide fraction and finds its target in Staphylococcus aureus.
METHODS
In this study the active fraction of P. trichocarpa crude extract was purified and characterized using MS/MS. This peptide PT13 antimicrobial activity was confirmed by in-vitro agar based disk diffusion and in-vivo infection model of G. mellonella. The proteomic expression analysis of S. aureus under influence of PT13 was studied using LTQ-Orbitrap-MS in-solution digestion and identity of target protein was acquired with their quantified expression using label-free approach of Progenesis QI software. Docking study was performed with peptide PT13 and its target YycG protein using CABS-dock.
RESULTS
The active fraction PT13 sequence was identified as KVPVAAAAAAAAAVVASSMVVAAAK, with 25 amino acid including 13 alanine having M/Z 2194.2469. PT13 was uniformly inhibited growth S. aureus SA91 and MIC was determined 16 μg/mL for SA91 S. aureus strain. Sensor histidine kinase (YycG) was most significant target found differentially expressed under influence of PT13. G. mellonella larvae were killed rapidly due to S aureus infection, whereas death in protected group was insignificant in compare to control. The docking models showed ten docking models with RMSD value 1.89 for cluster 1 and RMSD value 3.95 for cluster 2 which is predicted to be high quality model.
CONCLUSION
Alanine rich peptide could be useful in constructing as antimicrobial peptide for targeting extracellular Domain of Sensor Histidine Kinase YycG from S. aureus used in the study.
DOI: 10.1016/j.micpath.2018.05.010
PubMed: 29758266
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.</title><author><name sortKey="Al Akeel, Raid" sort="Al Akeel, Raid" uniqKey="Al Akeel R" first="Raid" last="Al Akeel">Raid Al Akeel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Mateen, Ayesha" sort="Mateen, Ayesha" uniqKey="Mateen A" first="Ayesha" last="Mateen">Ayesha Mateen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Syed, Rabbani" sort="Syed, Rabbani" uniqKey="Syed R" first="Rabbani" last="Syed">Rabbani Syed</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Electronic address: rsyed@ksu.edu.sa.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alqahtani, Mohammed S" sort="Alqahtani, Mohammed S" uniqKey="Alqahtani M" first="Mohammed S" last="Alqahtani">Mohammed S. Alqahtani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alqahtani, Ali S" sort="Alqahtani, Ali S" uniqKey="Alqahtani A" first="Ali S" last="Alqahtani">Ali S. Alqahtani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29758266</idno><idno type="pmid">29758266</idno><idno type="doi">10.1016/j.micpath.2018.05.010</idno><idno type="wicri:Area/Main/Corpus">000E36</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E36</idno><idno type="wicri:Area/Main/Curation">000E36</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E36</idno><idno type="wicri:Area/Main/Exploration">000E36</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.</title><author><name sortKey="Al Akeel, Raid" sort="Al Akeel, Raid" uniqKey="Al Akeel R" first="Raid" last="Al Akeel">Raid Al Akeel</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Mateen, Ayesha" sort="Mateen, Ayesha" uniqKey="Mateen A" first="Ayesha" last="Mateen">Ayesha Mateen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Syed, Rabbani" sort="Syed, Rabbani" uniqKey="Syed R" first="Rabbani" last="Syed">Rabbani Syed</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Electronic address: rsyed@ksu.edu.sa.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alqahtani, Mohammed S" sort="Alqahtani, Mohammed S" uniqKey="Alqahtani M" first="Mohammed S" last="Alqahtani">Mohammed S. Alqahtani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alqahtani, Ali S" sort="Alqahtani, Ali S" uniqKey="Alqahtani A" first="Ali S" last="Alqahtani">Ali S. Alqahtani</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbial pathogenesis</title><idno type="eISSN">1096-1208</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alanine (chemistry)</term><term>Antimicrobial Cationic Peptides (pharmacology)</term><term>Bacterial Proteins (antagonists & inhibitors)</term><term>Drug Resistance, Multiple, Bacterial (MeSH)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Gene Expression Regulation, Bacterial (MeSH)</term><term>Histidine Kinase (antagonists & inhibitors)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Molecular Docking Simulation (MeSH)</term><term>Populus (chemistry)</term><term>Protein Conformation (MeSH)</term><term>Proteomics (MeSH)</term><term>Staphylococcus aureus (drug effects)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alanine (composition chimique)</term><term>Antienzymes (pharmacologie)</term><term>Conformation des protéines (MeSH)</term><term>Histidine kinase (antagonistes et inhibiteurs)</term><term>Multirésistance bactérienne aux médicaments (MeSH)</term><term>Peptides antimicrobiens cationiques (pharmacologie)</term><term>Populus (composition chimique)</term><term>Protéines bactériennes (antagonistes et inhibiteurs)</term><term>Protéomique (MeSH)</term><term>Régulation de l'expression des gènes bactériens (MeSH)</term><term>Simulation de docking moléculaire (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Staphylococcus aureus (effets des médicaments et des substances chimiques)</term><term>Tests de sensibilité microbienne (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Bacterial Proteins</term><term>Histidine Kinase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alanine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antimicrobial Cationic Peptides</term><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Histidine kinase</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alanine</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Peptides antimicrobiens cationiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Drug Resistance, Multiple, Bacterial</term><term>Gene Expression Regulation, Bacterial</term><term>Microbial Sensitivity Tests</term><term>Molecular Docking Simulation</term><term>Protein Conformation</term><term>Proteomics</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Multirésistance bactérienne aux médicaments</term><term>Protéomique</term><term>Régulation de l'expression des gènes bactériens</term><term>Simulation de docking moléculaire</term><term>Spectrométrie de masse en tandem</term><term>Tests de sensibilité microbienne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Due to growing concern towards microbial resistance, ongoing search for developing novel bioactive compounds such as peptides is on rise. The aim of this study was to evaluate antimicrobial effect of Populus trichocarpa extract, chemically identify the active peptide fraction and finds its target in Staphylococcus aureus.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>In this study the active fraction of P. trichocarpa crude extract was purified and characterized using MS/MS. This peptide PT13 antimicrobial activity was confirmed by in-vitro agar based disk diffusion and in-vivo infection model of G. mellonella. The proteomic expression analysis of S. aureus under influence of PT13 was studied using LTQ-Orbitrap-MS in-solution digestion and identity of target protein was acquired with their quantified expression using label-free approach of Progenesis QI software. Docking study was performed with peptide PT13 and its target YycG protein using CABS-dock.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The active fraction PT13 sequence was identified as KVPVAAAAAAAAAVVASSMVVAAAK, with 25 amino acid including 13 alanine having M/Z 2194.2469. PT13 was uniformly inhibited growth S. aureus SA91 and MIC was determined 16 μg/mL for SA91 S. aureus strain. Sensor histidine kinase (YycG) was most significant target found differentially expressed under influence of PT13. G. mellonella larvae were killed rapidly due to S aureus infection, whereas death in protected group was insignificant in compare to control. The docking models showed ten docking models with RMSD value 1.89 for cluster 1 and RMSD value 3.95 for cluster 2 which is predicted to be high quality model.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Alanine rich peptide could be useful in constructing as antimicrobial peptide for targeting extracellular Domain of Sensor Histidine Kinase YycG from S. aureus used in the study.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29758266</PMID><DateCompleted><Year>2019</Year><Month>01</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1208</ISSN><JournalIssue CitedMedium="Internet"><Volume>121</Volume><PubDate><Year>2018</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Microbial pathogenesis</Title><ISOAbbreviation>Microb Pathog</ISOAbbreviation></Journal><ArticleTitle>Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.</ArticleTitle><Pagination><MedlinePgn>115-122</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0882-4010(18)30448-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micpath.2018.05.010</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Due to growing concern towards microbial resistance, ongoing search for developing novel bioactive compounds such as peptides is on rise. The aim of this study was to evaluate antimicrobial effect of Populus trichocarpa extract, chemically identify the active peptide fraction and finds its target in Staphylococcus aureus.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">In this study the active fraction of P. trichocarpa crude extract was purified and characterized using MS/MS. This peptide PT13 antimicrobial activity was confirmed by in-vitro agar based disk diffusion and in-vivo infection model of G. mellonella. The proteomic expression analysis of S. aureus under influence of PT13 was studied using LTQ-Orbitrap-MS in-solution digestion and identity of target protein was acquired with their quantified expression using label-free approach of Progenesis QI software. Docking study was performed with peptide PT13 and its target YycG protein using CABS-dock.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The active fraction PT13 sequence was identified as KVPVAAAAAAAAAVVASSMVVAAAK, with 25 amino acid including 13 alanine having M/Z 2194.2469. PT13 was uniformly inhibited growth S. aureus SA91 and MIC was determined 16 μg/mL for SA91 S. aureus strain. Sensor histidine kinase (YycG) was most significant target found differentially expressed under influence of PT13. G. mellonella larvae were killed rapidly due to S aureus infection, whereas death in protected group was insignificant in compare to control. The docking models showed ten docking models with RMSD value 1.89 for cluster 1 and RMSD value 3.95 for cluster 2 which is predicted to be high quality model.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Alanine rich peptide could be useful in constructing as antimicrobial peptide for targeting extracellular Domain of Sensor Histidine Kinase YycG from S. aureus used in the study.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Al Akeel</LastName><ForeName>Raid</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mateen</LastName><ForeName>Ayesha</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Syed</LastName><ForeName>Rabbani</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Electronic address: rsyed@ksu.edu.sa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alqahtani</LastName><ForeName>Mohammed S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alqahtani</LastName><ForeName>Ali S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microb Pathog</MedlineTA><NlmUniqueID>8606191</NlmUniqueID><ISSNLinking>0882-4010</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D023181">Antimicrobial Cationic Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.13.1</RegistryNumber><NameOfSubstance UI="D000071677">Histidine Kinase</NameOfSubstance></Chemical><Chemical><RegistryNumber>OF5P57N2ZX</RegistryNumber><NameOfSubstance UI="D000409">Alanine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000409" MajorTopicYN="N">Alanine</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023181" MajorTopicYN="N">Antimicrobial Cationic Peptides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024901" MajorTopicYN="N">Drug Resistance, Multiple, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000071677" MajorTopicYN="N">Histidine Kinase</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antimicrobial</Keyword><Keyword MajorTopicYN="N">CABS –dock</Keyword><Keyword MajorTopicYN="N">Populus trichocarpa</Keyword><Keyword MajorTopicYN="N">Protein –peptide</Keyword><Keyword MajorTopicYN="N">Staphylococcus aureus</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>1</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29758266</ArticleId><ArticleId IdType="pii">S0882-4010(18)30448-0</ArticleId><ArticleId IdType="doi">10.1016/j.micpath.2018.05.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Al Akeel, Raid" sort="Al Akeel, Raid" uniqKey="Al Akeel R" first="Raid" last="Al Akeel">Raid Al Akeel</name></noRegion><name sortKey="Alqahtani, Ali S" sort="Alqahtani, Ali S" uniqKey="Alqahtani A" first="Ali S" last="Alqahtani">Ali S. Alqahtani</name><name sortKey="Alqahtani, Mohammed S" sort="Alqahtani, Mohammed S" uniqKey="Alqahtani M" first="Mohammed S" last="Alqahtani">Mohammed S. Alqahtani</name><name sortKey="Mateen, Ayesha" sort="Mateen, Ayesha" uniqKey="Mateen A" first="Ayesha" last="Mateen">Ayesha Mateen</name><name sortKey="Syed, Rabbani" sort="Syed, Rabbani" uniqKey="Syed R" first="Rabbani" last="Syed">Rabbani Syed</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001062 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001062 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29758266
|texte= Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29758266" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |