Circulating TFH cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity.
Identifieur interne : 000F94 ( Main/Exploration ); précédent : 000F93; suivant : 000F95Circulating TFH cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity.
Auteurs : Marios Koutsakos [Australie] ; Adam K. Wheatley [Australie] ; Liyen Loh [Australie] ; E Bridie Clemens [Australie] ; Sneha Sant [Australie] ; Simone Nüssing [Australie] ; Annette Fox [Australie] ; Amy W. Chung [Australie] ; Karen L. Laurie [Australie] ; Aeron C. Hurt [Australie] ; Steve Rockman [Australie] ; Martha Lappas [Australie] ; Thomas Loudovaris [Australie] ; Stuart I. Mannering [Australie] ; Glen P. Westall [Australie] ; Michael Elliot [Australie] ; Stuart G. Tangye [Australie] ; Linda M. Wakim [Australie] ; Stephen J. Kent [Australie] ; Thi H O. Nguyen [Australie] ; Katherine Kedzierska [Australie]Source :
- Science translational medicine [ 1946-6242 ] ; 2018.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Anticorps antiviraux (immunologie), Antigènes CD (métabolisme), Cellules productrices d'anticorps (métabolisme), Glycoprotéine hémagglutinine du virus influenza (métabolisme), Grippe humaine (immunologie), Grippe humaine (sang), Humains (MeSH), Immunité cellulaire (MeSH), Lymphocytes B (immunologie), Lymphocytes T auxiliaires (immunologie), Mémoire immunologique (MeSH), Vaccination (MeSH), Vaccins inactivés (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Grippe humaine, Lymphocytes B, Lymphocytes T auxiliaires, Vaccins inactivés.
- métabolisme : Antigènes CD, Cellules productrices d'anticorps, Glycoprotéine hémagglutinine du virus influenza.
- sang : Grippe humaine.
- Adulte, Humains, Immunité cellulaire, Mémoire immunologique, Vaccination.
English descriptors
- KwdEn :
- Adult (MeSH), Antibodies, Viral (immunology), Antibody-Producing Cells (metabolism), Antigens, CD (metabolism), B-Lymphocytes (immunology), Hemagglutinin Glycoproteins, Influenza Virus (metabolism), Humans (MeSH), Immunity, Cellular (MeSH), Immunologic Memory (MeSH), Influenza, Human (blood), Influenza, Human (immunology), T-Lymphocytes, Helper-Inducer (immunology), Vaccination (MeSH), Vaccines, Inactivated (immunology).
- MESH :
- chemical , immunology : Antibodies, Viral, Vaccines, Inactivated.
- blood : Influenza, Human.
- immunology : B-Lymphocytes, Influenza, Human, T-Lymphocytes, Helper-Inducer.
- metabolism : Antibody-Producing Cells, Antigens, CD, Hemagglutinin Glycoproteins, Influenza Virus.
- Adult, Humans, Immunity, Cellular, Immunologic Memory, Vaccination.
Abstract
Immunization with the inactivated influenza vaccine (IIV) remains the most effective strategy to combat seasonal influenza infections. IIV activates B cells and T follicular helper (TFH) cells and thus engenders antibody-secreting cells and serum antibody titers. However, the cellular events preceding generation of protective immunity in humans are inadequately understood. We undertook an in-depth analysis of B cell and T cell immune responses to IIV in 35 healthy adults. Using recombinant hemagglutinin (rHA) probes to dissect the quantity, phenotype, and isotype of influenza-specific B cells against A/California09-H1N1, A/Switzerland-H3N2, and B/Phuket, we showed that vaccination induced a three-pronged B cell response comprising a transient CXCR5-CXCR3+ antibody-secreting B cell population, CD21hiCD27+ memory B cells, and CD21loCD27+ B cells. Activation of circulating TFH cells correlated with the development of both CD21lo and CD21hi memory B cells. However, preexisting antibodies could limit increases in serum antibody titers. IIV had no marked effect on CD8+, mucosal-associated invariant T, γδ T, and natural killer cell activation. In addition, vaccine-induced B cells were not maintained in peripheral blood at 1 year after vaccination. We provide a dissection of rHA-specific B cells across seven human tissue compartments, showing that influenza-specific memory (CD21hiCD27+) B cells primarily reside within secondary lymphoid tissues and the lungs. Our study suggests that a rational design of universal vaccines needs to consider circulating TFH cells, preexisting serological memory, and tissue compartmentalization for effective B cell immunity, as well as to improve targeting cellular T cell immunity.
DOI: 10.1126/scitranslmed.aan8405
PubMed: 29444980
Affiliations:
- Australie
- Nouvelle-Galles du Sud, Victoria (État)
- Melbourne, Sydney
- Université de Melbourne, Université de Sydney
Links toward previous steps (curation, corpus...)
Le document en format XML
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Chung</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Laurie, Karen L" sort="Laurie, Karen L" uniqKey="Laurie K" first="Karen L" last="Laurie">Karen L. Laurie</name><affiliation wicri:level="1"><nlm:affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000</wicri:regionArea><wicri:noRegion>Victoria 3000</wicri:noRegion></affiliation></author><author><name sortKey="Hurt, Aeron C" sort="Hurt, Aeron C" uniqKey="Hurt A" first="Aeron C" last="Hurt">Aeron C. Hurt</name><affiliation wicri:level="1"><nlm:affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000</wicri:regionArea><wicri:noRegion>Victoria 3000</wicri:noRegion></affiliation></author><author><name sortKey="Rockman, Steve" sort="Rockman, Steve" uniqKey="Rockman S" first="Steve" last="Rockman">Steve Rockman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Road, Parkville, Victoria 3052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Road, Parkville, Victoria 3052</wicri:regionArea><wicri:noRegion>Victoria 3052</wicri:noRegion></affiliation></author><author><name sortKey="Lappas, Martha" sort="Lappas, Martha" uniqKey="Lappas M" first="Martha" last="Lappas">Martha Lappas</name><affiliation wicri:level="4"><nlm:affiliation>Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria 3084, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria 3084</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Loudovaris, Thomas" sort="Loudovaris, Thomas" uniqKey="Loudovaris T" first="Thomas" last="Loudovaris">Thomas Loudovaris</name><affiliation wicri:level="1"><nlm:affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065</wicri:regionArea><wicri:noRegion>Victoria 3065</wicri:noRegion></affiliation></author><author><name sortKey="Mannering, Stuart I" sort="Mannering, Stuart I" uniqKey="Mannering S" first="Stuart I" last="Mannering">Stuart I. Mannering</name><affiliation wicri:level="1"><nlm:affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065</wicri:regionArea><wicri:noRegion>Victoria 3065</wicri:noRegion></affiliation></author><author><name sortKey="Westall, Glen P" sort="Westall, Glen P" uniqKey="Westall G" first="Glen P" last="Westall">Glen P. Westall</name><affiliation wicri:level="1"><nlm:affiliation>Lung Transplant Unit, Alfred Hospital, Melbourne, Victoria 3004, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Lung Transplant Unit, Alfred Hospital, Melbourne, Victoria 3004</wicri:regionArea><wicri:noRegion>Victoria 3004</wicri:noRegion></affiliation></author><author><name sortKey="Elliot, Michael" sort="Elliot, Michael" uniqKey="Elliot M" first="Michael" last="Elliot">Michael Elliot</name><affiliation wicri:level="4"><nlm:affiliation>Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Sydney Medical School, University of Sydney, Sydney, New South Wales 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Chris O'Brien Lifehouse Cancer Centre, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Chris O'Brien Lifehouse Cancer Centre, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050</wicri:regionArea><wicri:noRegion>New South Wales 2050</wicri:noRegion></affiliation></author><author><name sortKey="Tangye, Stuart G" sort="Tangye, Stuart G" uniqKey="Tangye S" first="Stuart G" last="Tangye">Stuart G. Tangye</name><affiliation wicri:level="1"><nlm:affiliation>Immunology Division, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology Division, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010</wicri:regionArea><wicri:noRegion>New South Wales 2010</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales 2052</wicri:regionArea><wicri:noRegion>New South Wales 2052</wicri:noRegion></affiliation></author><author><name sortKey="Wakim, Linda M" sort="Wakim, Linda M" uniqKey="Wakim L" first="Linda M" last="Wakim">Linda M. Wakim</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004</wicri:regionArea><wicri:noRegion>Victoria 3004</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H O" last="Nguyen">Thi H O. Nguyen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29444980</idno><idno type="pmid">29444980</idno><idno type="doi">10.1126/scitranslmed.aan8405</idno><idno type="wicri:Area/Main/Corpus">000F63</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000F63</idno><idno type="wicri:Area/Main/Curation">000F63</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000F63</idno><idno type="wicri:Area/Main/Exploration">000F63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Circulating T<sub>FH</sub> cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity.</title><author><name sortKey="Koutsakos, Marios" sort="Koutsakos, Marios" uniqKey="Koutsakos M" first="Marios" last="Koutsakos">Marios Koutsakos</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Wheatley, Adam K" sort="Wheatley, Adam K" uniqKey="Wheatley A" first="Adam K" last="Wheatley">Adam K. Wheatley</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Loh, Liyen" sort="Loh, Liyen" uniqKey="Loh L" first="Liyen" last="Loh">Liyen Loh</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Clemens, E Bridie" sort="Clemens, E Bridie" uniqKey="Clemens E" first="E Bridie" last="Clemens">E Bridie Clemens</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Sant, Sneha" sort="Sant, Sneha" uniqKey="Sant S" first="Sneha" last="Sant">Sneha Sant</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Nussing, Simone" sort="Nussing, Simone" uniqKey="Nussing S" first="Simone" last="Nüssing">Simone Nüssing</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Fox, Annette" sort="Fox, Annette" uniqKey="Fox A" first="Annette" last="Fox">Annette Fox</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Chung, Amy W" sort="Chung, Amy W" uniqKey="Chung A" first="Amy W" last="Chung">Amy W. Chung</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Laurie, Karen L" sort="Laurie, Karen L" uniqKey="Laurie K" first="Karen L" last="Laurie">Karen L. Laurie</name><affiliation wicri:level="1"><nlm:affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000</wicri:regionArea><wicri:noRegion>Victoria 3000</wicri:noRegion></affiliation></author><author><name sortKey="Hurt, Aeron C" sort="Hurt, Aeron C" uniqKey="Hurt A" first="Aeron C" last="Hurt">Aeron C. Hurt</name><affiliation wicri:level="1"><nlm:affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000</wicri:regionArea><wicri:noRegion>Victoria 3000</wicri:noRegion></affiliation></author><author><name sortKey="Rockman, Steve" sort="Rockman, Steve" uniqKey="Rockman S" first="Steve" last="Rockman">Steve Rockman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Road, Parkville, Victoria 3052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Road, Parkville, Victoria 3052</wicri:regionArea><wicri:noRegion>Victoria 3052</wicri:noRegion></affiliation></author><author><name sortKey="Lappas, Martha" sort="Lappas, Martha" uniqKey="Lappas M" first="Martha" last="Lappas">Martha Lappas</name><affiliation wicri:level="4"><nlm:affiliation>Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria 3084, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria 3084</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Loudovaris, Thomas" sort="Loudovaris, Thomas" uniqKey="Loudovaris T" first="Thomas" last="Loudovaris">Thomas Loudovaris</name><affiliation wicri:level="1"><nlm:affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065</wicri:regionArea><wicri:noRegion>Victoria 3065</wicri:noRegion></affiliation></author><author><name sortKey="Mannering, Stuart I" sort="Mannering, Stuart I" uniqKey="Mannering S" first="Stuart I" last="Mannering">Stuart I. Mannering</name><affiliation wicri:level="1"><nlm:affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065</wicri:regionArea><wicri:noRegion>Victoria 3065</wicri:noRegion></affiliation></author><author><name sortKey="Westall, Glen P" sort="Westall, Glen P" uniqKey="Westall G" first="Glen P" last="Westall">Glen P. Westall</name><affiliation wicri:level="1"><nlm:affiliation>Lung Transplant Unit, Alfred Hospital, Melbourne, Victoria 3004, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Lung Transplant Unit, Alfred Hospital, Melbourne, Victoria 3004</wicri:regionArea><wicri:noRegion>Victoria 3004</wicri:noRegion></affiliation></author><author><name sortKey="Elliot, Michael" sort="Elliot, Michael" uniqKey="Elliot M" first="Michael" last="Elliot">Michael Elliot</name><affiliation wicri:level="4"><nlm:affiliation>Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Sydney Medical School, University of Sydney, Sydney, New South Wales 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Chris O'Brien Lifehouse Cancer Centre, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Chris O'Brien Lifehouse Cancer Centre, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050</wicri:regionArea><wicri:noRegion>New South Wales 2050</wicri:noRegion></affiliation></author><author><name sortKey="Tangye, Stuart G" sort="Tangye, Stuart G" uniqKey="Tangye S" first="Stuart G" last="Tangye">Stuart G. Tangye</name><affiliation wicri:level="1"><nlm:affiliation>Immunology Division, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Immunology Division, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010</wicri:regionArea><wicri:noRegion>New South Wales 2010</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales 2052</wicri:regionArea><wicri:noRegion>New South Wales 2052</wicri:noRegion></affiliation></author><author><name sortKey="Wakim, Linda M" sort="Wakim, Linda M" uniqKey="Wakim L" first="Linda M" last="Wakim">Linda M. Wakim</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004</wicri:regionArea><wicri:noRegion>Victoria 3004</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H O" last="Nguyen">Thi H O. Nguyen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><series><title level="j">Science translational medicine</title><idno type="eISSN">1946-6242</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Antibodies, Viral (immunology)</term><term>Antibody-Producing Cells (metabolism)</term><term>Antigens, CD (metabolism)</term><term>B-Lymphocytes (immunology)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (metabolism)</term><term>Humans (MeSH)</term><term>Immunity, Cellular (MeSH)</term><term>Immunologic Memory (MeSH)</term><term>Influenza, Human (blood)</term><term>Influenza, Human (immunology)</term><term>T-Lymphocytes, Helper-Inducer (immunology)</term><term>Vaccination (MeSH)</term><term>Vaccines, Inactivated (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term><term>Anticorps antiviraux (immunologie)</term><term>Antigènes CD (métabolisme)</term><term>Cellules productrices d'anticorps (métabolisme)</term><term>Glycoprotéine hémagglutinine du virus influenza (métabolisme)</term><term>Grippe humaine (immunologie)</term><term>Grippe humaine (sang)</term><term>Humains (MeSH)</term><term>Immunité cellulaire (MeSH)</term><term>Lymphocytes B (immunologie)</term><term>Lymphocytes T auxiliaires (immunologie)</term><term>Mémoire immunologique (MeSH)</term><term>Vaccination (MeSH)</term><term>Vaccins inactivés (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Vaccines, Inactivated</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Grippe humaine</term><term>Lymphocytes B</term><term>Lymphocytes T auxiliaires</term><term>Vaccins inactivés</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Influenza, Human</term><term>T-Lymphocytes, Helper-Inducer</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Antibody-Producing Cells</term><term>Antigens, CD</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes CD</term><term>Cellules productrices d'anticorps</term><term>Glycoprotéine hémagglutinine du virus influenza</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Humans</term><term>Immunity, Cellular</term><term>Immunologic Memory</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Humains</term><term>Immunité cellulaire</term><term>Mémoire immunologique</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Immunization with the inactivated influenza vaccine (IIV) remains the most effective strategy to combat seasonal influenza infections. IIV activates B cells and T follicular helper (T<sub>FH</sub>) cells and thus engenders antibody-secreting cells and serum antibody titers. However, the cellular events preceding generation of protective immunity in humans are inadequately understood. We undertook an in-depth analysis of B cell and T cell immune responses to IIV in 35 healthy adults. Using recombinant hemagglutinin (rHA) probes to dissect the quantity, phenotype, and isotype of influenza-specific B cells against A/California09-H1N1, A/Switzerland-H3N2, and B/Phuket, we showed that vaccination induced a three-pronged B cell response comprising a transient CXCR5<sup>-</sup>CXCR3<sup>+</sup> antibody-secreting B cell population, CD21<sup>hi</sup>CD27<sup>+</sup> memory B cells, and CD21<sup>lo</sup>CD27<sup>+</sup> B cells. Activation of circulating T<sub>FH</sub> cells correlated with the development of both CD21<sup>lo</sup> and CD21<sup>hi</sup> memory B cells. However, preexisting antibodies could limit increases in serum antibody titers. IIV had no marked effect on CD8<sup>+</sup>, mucosal-associated invariant T, γδ T, and natural killer cell activation. In addition, vaccine-induced B cells were not maintained in peripheral blood at 1 year after vaccination. We provide a dissection of rHA-specific B cells across seven human tissue compartments, showing that influenza-specific memory (CD21<sup>hi</sup>CD27<sup>+</sup>) B cells primarily reside within secondary lymphoid tissues and the lungs. Our study suggests that a rational design of universal vaccines needs to consider circulating T<sub>FH</sub> cells, preexisting serological memory, and tissue compartmentalization for effective B cell immunity, as well as to improve targeting cellular T cell immunity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29444980</PMID><DateCompleted><Year>2019</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1946-6242</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>428</Issue><PubDate><Year>2018</Year><Month>02</Month><Day>14</Day></PubDate></JournalIssue><Title>Science translational medicine</Title><ISOAbbreviation>Sci Transl Med</ISOAbbreviation></Journal><ArticleTitle>Circulating T<sub>FH</sub> cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">eaan8405</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1126/scitranslmed.aan8405</ELocationID><Abstract><AbstractText>Immunization with the inactivated influenza vaccine (IIV) remains the most effective strategy to combat seasonal influenza infections. IIV activates B cells and T follicular helper (T<sub>FH</sub>) cells and thus engenders antibody-secreting cells and serum antibody titers. However, the cellular events preceding generation of protective immunity in humans are inadequately understood. We undertook an in-depth analysis of B cell and T cell immune responses to IIV in 35 healthy adults. Using recombinant hemagglutinin (rHA) probes to dissect the quantity, phenotype, and isotype of influenza-specific B cells against A/California09-H1N1, A/Switzerland-H3N2, and B/Phuket, we showed that vaccination induced a three-pronged B cell response comprising a transient CXCR5<sup>-</sup>CXCR3<sup>+</sup> antibody-secreting B cell population, CD21<sup>hi</sup>CD27<sup>+</sup> memory B cells, and CD21<sup>lo</sup>CD27<sup>+</sup> B cells. Activation of circulating T<sub>FH</sub> cells correlated with the development of both CD21<sup>lo</sup> and CD21<sup>hi</sup> memory B cells. However, preexisting antibodies could limit increases in serum antibody titers. IIV had no marked effect on CD8<sup>+</sup>, mucosal-associated invariant T, γδ T, and natural killer cell activation. In addition, vaccine-induced B cells were not maintained in peripheral blood at 1 year after vaccination. We provide a dissection of rHA-specific B cells across seven human tissue compartments, showing that influenza-specific memory (CD21<sup>hi</sup>CD27<sup>+</sup>) B cells primarily reside within secondary lymphoid tissues and the lungs. Our study suggests that a rational design of universal vaccines needs to consider circulating T<sub>FH</sub> cells, preexisting serological memory, and tissue compartmentalization for effective B cell immunity, as well as to improve targeting cellular T cell immunity.</AbstractText><CopyrightInformation>Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koutsakos</LastName><ForeName>Marios</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0001-9994-9723</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wheatley</LastName><ForeName>Adam K</ForeName><Initials>AK</Initials><Identifier Source="ORCID">0000-0002-5593-9387</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Loh</LastName><ForeName>Liyen</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Clemens</LastName><ForeName>E Bridie</ForeName><Initials>EB</Initials><Identifier Source="ORCID">0000-0002-8473-4743</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sant</LastName><ForeName>Sneha</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nüssing</LastName><ForeName>Simone</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-0685-3413</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fox</LastName><ForeName>Annette</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-0565-7146</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chung</LastName><ForeName>Amy W</ForeName><Initials>AW</Initials><Identifier Source="ORCID">0000-0003-0020-9704</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laurie</LastName><ForeName>Karen L</ForeName><Initials>KL</Initials><AffiliationInfo><Affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hurt</LastName><ForeName>Aeron C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>World Health Organization (WHO) Collaborating Centre for Reference and Research on Influenza, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rockman</LastName><ForeName>Steve</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Seqirus, 63 Poplar Road, Parkville, Victoria 3052, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lappas</LastName><ForeName>Martha</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria 3084, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Loudovaris</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0002-7038-2302</Identifier><AffiliationInfo><Affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mannering</LastName><ForeName>Stuart I</ForeName><Initials>SI</Initials><Identifier Source="ORCID">0000-0003-3497-3559</Identifier><AffiliationInfo><Affiliation>Immunology and Diabetes Unit, St Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Westall</LastName><ForeName>Glen P</ForeName><Initials>GP</Initials><AffiliationInfo><Affiliation>Lung Transplant Unit, Alfred Hospital, Melbourne, Victoria 3004, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elliot</LastName><ForeName>Michael</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0001-5654-2114</Identifier><AffiliationInfo><Affiliation>Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Chris O'Brien Lifehouse Cancer Centre, Royal Prince Alfred Hospital, Camperdown, New South Wales 2050, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tangye</LastName><ForeName>Stuart G</ForeName><Initials>SG</Initials><Identifier Source="ORCID">0000-0002-5360-5180</Identifier><AffiliationInfo><Affiliation>Immunology Division, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wakim</LastName><ForeName>Linda M</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kent</LastName><ForeName>Stephen J</ForeName><Initials>SJ</Initials><Identifier Source="ORCID">0000-0002-8539-4891</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Thi H O</ForeName><Initials>THO</Initials><Identifier Source="ORCID">0000-0002-9294-7693</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kedzierska</LastName><ForeName>Katherine</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0001-6141-335X</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Melbourne, The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia. kkedz@unimelb.edu.au tho.nguyen@unimelb.edu.au.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Sci Transl Med</MedlineTA><NlmUniqueID>101505086</NlmUniqueID><ISSNLinking>1946-6234</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015703">Antigens, CD</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015164">Vaccines, Inactivated</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000921" MajorTopicYN="N">Antibody-Producing Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015703" MajorTopicYN="N">Antigens, CD</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001402" MajorTopicYN="N">B-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007111" MajorTopicYN="Y">Immunity, Cellular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007156" MajorTopicYN="Y">Immunologic Memory</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006377" MajorTopicYN="N">T-Lymphocytes, Helper-Inducer</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015164" MajorTopicYN="N">Vaccines, Inactivated</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>2</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29444980</ArticleId><ArticleId IdType="pii">10/428/eaan8405</ArticleId><ArticleId IdType="doi">10.1126/scitranslmed.aan8405</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li><li>Victoria (État)</li></region><settlement><li>Melbourne</li><li>Sydney</li></settlement><orgName><li>Université de Melbourne</li><li>Université de Sydney</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Koutsakos, Marios" sort="Koutsakos, Marios" uniqKey="Koutsakos M" first="Marios" last="Koutsakos">Marios Koutsakos</name></region><name sortKey="Chung, Amy W" sort="Chung, Amy W" uniqKey="Chung A" first="Amy W" last="Chung">Amy W. Chung</name><name sortKey="Clemens, E Bridie" sort="Clemens, E Bridie" uniqKey="Clemens E" first="E Bridie" last="Clemens">E Bridie Clemens</name><name sortKey="Elliot, Michael" sort="Elliot, Michael" uniqKey="Elliot M" first="Michael" last="Elliot">Michael Elliot</name><name sortKey="Elliot, Michael" sort="Elliot, Michael" uniqKey="Elliot M" first="Michael" last="Elliot">Michael Elliot</name><name sortKey="Fox, Annette" sort="Fox, Annette" uniqKey="Fox A" first="Annette" last="Fox">Annette Fox</name><name sortKey="Hurt, Aeron C" sort="Hurt, Aeron C" uniqKey="Hurt A" first="Aeron C" last="Hurt">Aeron C. Hurt</name><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name><name sortKey="Lappas, Martha" sort="Lappas, Martha" uniqKey="Lappas M" first="Martha" last="Lappas">Martha Lappas</name><name sortKey="Laurie, Karen L" sort="Laurie, Karen L" uniqKey="Laurie K" first="Karen L" last="Laurie">Karen L. Laurie</name><name sortKey="Loh, Liyen" sort="Loh, Liyen" uniqKey="Loh L" first="Liyen" last="Loh">Liyen Loh</name><name sortKey="Loudovaris, Thomas" sort="Loudovaris, Thomas" uniqKey="Loudovaris T" first="Thomas" last="Loudovaris">Thomas Loudovaris</name><name sortKey="Mannering, Stuart I" sort="Mannering, Stuart I" uniqKey="Mannering S" first="Stuart I" last="Mannering">Stuart I. Mannering</name><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H O" last="Nguyen">Thi H O. Nguyen</name><name sortKey="Nussing, Simone" sort="Nussing, Simone" uniqKey="Nussing S" first="Simone" last="Nüssing">Simone Nüssing</name><name sortKey="Rockman, Steve" sort="Rockman, Steve" uniqKey="Rockman S" first="Steve" last="Rockman">Steve Rockman</name><name sortKey="Rockman, Steve" sort="Rockman, Steve" uniqKey="Rockman S" first="Steve" last="Rockman">Steve Rockman</name><name sortKey="Sant, Sneha" sort="Sant, Sneha" uniqKey="Sant S" first="Sneha" last="Sant">Sneha Sant</name><name sortKey="Tangye, Stuart G" sort="Tangye, Stuart G" uniqKey="Tangye S" first="Stuart G" last="Tangye">Stuart G. Tangye</name><name sortKey="Tangye, Stuart G" sort="Tangye, Stuart G" uniqKey="Tangye S" first="Stuart G" last="Tangye">Stuart G. Tangye</name><name sortKey="Wakim, Linda M" sort="Wakim, Linda M" uniqKey="Wakim L" first="Linda M" last="Wakim">Linda M. Wakim</name><name sortKey="Westall, Glen P" sort="Westall, Glen P" uniqKey="Westall G" first="Glen P" last="Westall">Glen P. Westall</name><name sortKey="Wheatley, Adam K" sort="Wheatley, Adam K" uniqKey="Wheatley A" first="Adam K" last="Wheatley">Adam K. Wheatley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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