ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) affects development, photosynthesis, and hormonal homeostasis in hybrid aspen (Populus tremula L. × P. tremuloides).
Identifieur interne : 000F45 ( Main/Exploration ); précédent : 000F44; suivant : 000F46ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) affects development, photosynthesis, and hormonal homeostasis in hybrid aspen (Populus tremula L. × P. tremuloides).
Auteurs : Maciej Jerzy Bernacki [Pologne] ; Weronika Czarnocka [Pologne] ; Damian Wito [Pologne] ; Anna Rusaczonek [Pologne] ; Magdalena Szechy Ska-Hebda [Pologne] ; Ireneusz Lesak [Pologne] ; Joanna D Browska-Bronk [Pologne] ; Stanisław Karpi Ski [Pologne]Source :
- Journal of plant physiology [ 1618-1328 ] ; 2018.
Descripteurs français
- KwdFr :
- Espèces réactives de l'oxygène (métabolisme), Facteur de croissance végétal (métabolisme), Homéostasie (génétique), Hybridation génétique (MeSH), Photosynthèse (génétique), Populus (croissance et développement), Populus (génétique), Populus (physiologie), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (physiologie).
- MESH :
- croissance et développement : Populus.
- génétique : Homéostasie, Photosynthèse, Populus, Protéines de liaison à l'ADN, Protéines végétales.
- métabolisme : Espèces réactives de l'oxygène, Facteur de croissance végétal, Protéines de liaison à l'ADN, Protéines végétales.
- physiologie : Populus, Régulation de l'expression des gènes végétaux.
- Hybridation génétique.
English descriptors
- KwdEn :
- DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Gene Expression Regulation, Plant (physiology), Homeostasis (genetics), Hybridization, Genetic (MeSH), Photosynthesis (genetics), Plant Growth Regulators (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (growth & development), Populus (physiology), Reactive Oxygen Species (metabolism).
- MESH :
- chemical , genetics : DNA-Binding Proteins, Plant Proteins.
- chemical , metabolism : DNA-Binding Proteins, Plant Growth Regulators, Plant Proteins, Reactive Oxygen Species.
- genetics : Homeostasis, Photosynthesis, Populus.
- growth & development : Populus.
- physiology : Gene Expression Regulation, Plant, Populus.
- Hybridization, Genetic.
Abstract
ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) was first described as a protein involved in salicylic acid (SA)-, ethylene-, and reactive oxygen species (ROS)-dependent defense and acclimation responses. It is a molecular regulator of biotic and abiotic stress-induced programmed cell death. Its role is relatively well known in annual plants, such as Arabidopsis thaliana or Nicotiana benthamiana. However, little is known about its functions in woody plants. Therefore, in this study, we aimed to characterize the function of EDS1 in the Populus tremula L. × P. tremuloides hybrid grown for several seasons in the natural environment. We used two transgenic lines, eds1-7 and eds1-12, with decreased EDS1 expression levels in this study. The observed changes in physiological and biochemical parameters corresponded with the EDS1 silencing level. Both transgenic lines produced more lateral shoots in comparison to the wild-type (WT) plants, which resulted in the modification of tree morphology. Photosynthetic parameters, such as quantum yield of photosystem II (ϕPSII), photochemical and non-photochemical quenching (qP and NPQ, respectively), as well as chlorophyll content were found to be increased in both transgenic lines, which resulted in changes in photosynthetic efficiency. Our data also revealed lower foliar concentrations of SA and ROS, the latter resulting most probably from more efficient antioxidant system in both transgenic lines. In addition, our data indicated significantly decreased rate of leaf senescence during several autumn seasons. Transcriptomic analysis revealed deregulation of 2215 and 376 genes in eds1-12 and eds1-7, respectively, and also revealed 207 genes that were commonly deregulated in both transgenic lines. The deregulation was primarily observed in the genes involved in photosynthesis, signaling, hormonal metabolism, and development, which was found to agree with the results of biochemical and physiological tests. In general, our data proved that poplar EDS1 affects tree morphology, photosynthetic efficiency, ROS and SA metabolism, as well as leaf senescence.
DOI: 10.1016/j.jplph.2018.04.014
PubMed: 29730441
Affiliations:
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Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Wito, Damian" sort="Wito, Damian" uniqKey="Wito D" first="Damian" last="Wito">Damian Wito</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Rusaczonek, Anna" sort="Rusaczonek, Anna" uniqKey="Rusaczonek A" first="Anna" last="Rusaczonek">Anna Rusaczonek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Szechy Ska Hebda, Magdalena" sort="Szechy Ska Hebda, Magdalena" uniqKey="Szechy Ska Hebda M" first="Magdalena" last="Szechy Ska-Hebda">Magdalena Szechy Ska-Hebda</name><affiliation wicri:level="1"><nlm:affiliation>The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland; Plant Breeding and Acclimatization Institute, 05-870 Błonie, Radzików, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland; Plant Breeding and Acclimatization Institute, 05-870 Błonie, Radzików</wicri:regionArea><wicri:noRegion>Radzików</wicri:noRegion></affiliation></author><author><name sortKey=" Lesak, Ireneusz" sort=" Lesak, Ireneusz" uniqKey=" Lesak I" first="Ireneusz" last=" Lesak">Ireneusz Lesak</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow</wicri:regionArea><wicri:noRegion>30-001 Cracow</wicri:noRegion></affiliation></author><author><name sortKey="D Browska Bronk, Joanna" sort="D Browska Bronk, Joanna" uniqKey="D Browska Bronk J" first="Joanna" last="D Browska-Bronk">Joanna D Browska-Bronk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Karpi Ski, Stanislaw" sort="Karpi Ski, Stanislaw" uniqKey="Karpi Ski S" first="Stanisław" last="Karpi Ski">Stanisław Karpi Ski</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland. Electronic address: stanislaw_karpinski@sggw.pl.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29730441</idno><idno type="pmid">29730441</idno><idno type="doi">10.1016/j.jplph.2018.04.014</idno><idno type="wicri:Area/Main/Corpus">000E46</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E46</idno><idno type="wicri:Area/Main/Curation">000E46</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E46</idno><idno type="wicri:Area/Main/Exploration">000E46</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) affects development, photosynthesis, and hormonal homeostasis in hybrid aspen (Populus tremula L. × P. tremuloides).</title><author><name sortKey="Bernacki, Maciej Jerzy" sort="Bernacki, Maciej Jerzy" uniqKey="Bernacki M" first="Maciej Jerzy" last="Bernacki">Maciej Jerzy Bernacki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Czarnocka, Weronika" sort="Czarnocka, Weronika" uniqKey="Czarnocka W" first="Weronika" last="Czarnocka">Weronika Czarnocka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Wito, Damian" sort="Wito, Damian" uniqKey="Wito D" first="Damian" last="Wito">Damian Wito</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Rusaczonek, Anna" sort="Rusaczonek, Anna" uniqKey="Rusaczonek A" first="Anna" last="Rusaczonek">Anna Rusaczonek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Szechy Ska Hebda, Magdalena" sort="Szechy Ska Hebda, Magdalena" uniqKey="Szechy Ska Hebda M" first="Magdalena" last="Szechy Ska-Hebda">Magdalena Szechy Ska-Hebda</name><affiliation wicri:level="1"><nlm:affiliation>The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland; Plant Breeding and Acclimatization Institute, 05-870 Błonie, Radzików, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland; Plant Breeding and Acclimatization Institute, 05-870 Błonie, Radzików</wicri:regionArea><wicri:noRegion>Radzików</wicri:noRegion></affiliation></author><author><name sortKey=" Lesak, Ireneusz" sort=" Lesak, Ireneusz" uniqKey=" Lesak I" first="Ireneusz" last=" Lesak">Ireneusz Lesak</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow</wicri:regionArea><wicri:noRegion>30-001 Cracow</wicri:noRegion></affiliation></author><author><name sortKey="D Browska Bronk, Joanna" sort="D Browska Bronk, Joanna" uniqKey="D Browska Bronk J" first="Joanna" last="D Browska-Bronk">Joanna D Browska-Bronk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Karpi Ski, Stanislaw" sort="Karpi Ski, Stanislaw" uniqKey="Karpi Ski S" first="Stanisław" last="Karpi Ski">Stanisław Karpi Ski</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland. Electronic address: stanislaw_karpinski@sggw.pl.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa</wicri:regionArea><wicri:noRegion>02-776 Warszawa</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA-Binding Proteins (genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Homeostasis (genetics)</term><term>Hybridization, Genetic (MeSH)</term><term>Photosynthesis (genetics)</term><term>Plant Growth Regulators (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (physiology)</term><term>Reactive Oxygen Species (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Facteur de croissance végétal (métabolisme)</term><term>Homéostasie (génétique)</term><term>Hybridation génétique (MeSH)</term><term>Photosynthèse (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (physiologie)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Binding Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Binding Proteins</term><term>Plant Growth Regulators</term><term>Plant Proteins</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Homeostasis</term><term>Photosynthesis</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Homéostasie</term><term>Photosynthèse</term><term>Populus</term><term>Protéines de liaison à l'ADN</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term><term>Facteur de croissance végétal</term><term>Protéines de liaison à l'ADN</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hybridization, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hybridation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) was first described as a protein involved in salicylic acid (SA)-, ethylene-, and reactive oxygen species (ROS)-dependent defense and acclimation responses. It is a molecular regulator of biotic and abiotic stress-induced programmed cell death. Its role is relatively well known in annual plants, such as Arabidopsis thaliana or Nicotiana benthamiana. However, little is known about its functions in woody plants. Therefore, in this study, we aimed to characterize the function of EDS1 in the Populus tremula L. × P. tremuloides hybrid grown for several seasons in the natural environment. We used two transgenic lines, eds1-7 and eds1-12, with decreased EDS1 expression levels in this study. The observed changes in physiological and biochemical parameters corresponded with the EDS1 silencing level. Both transgenic lines produced more lateral shoots in comparison to the wild-type (WT) plants, which resulted in the modification of tree morphology. Photosynthetic parameters, such as quantum yield of photosystem II (ϕPSII), photochemical and non-photochemical quenching (qP and NPQ, respectively), as well as chlorophyll content were found to be increased in both transgenic lines, which resulted in changes in photosynthetic efficiency. Our data also revealed lower foliar concentrations of SA and ROS, the latter resulting most probably from more efficient antioxidant system in both transgenic lines. In addition, our data indicated significantly decreased rate of leaf senescence during several autumn seasons. Transcriptomic analysis revealed deregulation of 2215 and 376 genes in eds1-12 and eds1-7, respectively, and also revealed 207 genes that were commonly deregulated in both transgenic lines. The deregulation was primarily observed in the genes involved in photosynthesis, signaling, hormonal metabolism, and development, which was found to agree with the results of biochemical and physiological tests. In general, our data proved that poplar EDS1 affects tree morphology, photosynthetic efficiency, ROS and SA metabolism, as well as leaf senescence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29730441</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>226</Volume><PubDate><Year>2018</Year><Month>07</Month></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) affects development, photosynthesis, and hormonal homeostasis in hybrid aspen (Populus tremula L. × P. tremuloides).</ArticleTitle><Pagination><MedlinePgn>91-102</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(18)30123-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2018.04.014</ELocationID><Abstract><AbstractText>ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) was first described as a protein involved in salicylic acid (SA)-, ethylene-, and reactive oxygen species (ROS)-dependent defense and acclimation responses. It is a molecular regulator of biotic and abiotic stress-induced programmed cell death. Its role is relatively well known in annual plants, such as Arabidopsis thaliana or Nicotiana benthamiana. However, little is known about its functions in woody plants. Therefore, in this study, we aimed to characterize the function of EDS1 in the Populus tremula L. × P. tremuloides hybrid grown for several seasons in the natural environment. We used two transgenic lines, eds1-7 and eds1-12, with decreased EDS1 expression levels in this study. The observed changes in physiological and biochemical parameters corresponded with the EDS1 silencing level. Both transgenic lines produced more lateral shoots in comparison to the wild-type (WT) plants, which resulted in the modification of tree morphology. Photosynthetic parameters, such as quantum yield of photosystem II (ϕPSII), photochemical and non-photochemical quenching (qP and NPQ, respectively), as well as chlorophyll content were found to be increased in both transgenic lines, which resulted in changes in photosynthetic efficiency. Our data also revealed lower foliar concentrations of SA and ROS, the latter resulting most probably from more efficient antioxidant system in both transgenic lines. In addition, our data indicated significantly decreased rate of leaf senescence during several autumn seasons. Transcriptomic analysis revealed deregulation of 2215 and 376 genes in eds1-12 and eds1-7, respectively, and also revealed 207 genes that were commonly deregulated in both transgenic lines. The deregulation was primarily observed in the genes involved in photosynthesis, signaling, hormonal metabolism, and development, which was found to agree with the results of biochemical and physiological tests. In general, our data proved that poplar EDS1 affects tree morphology, photosynthetic efficiency, ROS and SA metabolism, as well as leaf senescence.</AbstractText><CopyrightInformation>Copyright © 2018. Published by Elsevier GmbH.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bernacki</LastName><ForeName>Maciej Jerzy</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Czarnocka</LastName><ForeName>Weronika</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Witoń</LastName><ForeName>Damian</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rusaczonek</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Szechyńska-Hebda</LastName><ForeName>Magdalena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland; Plant Breeding and Acclimatization Institute, 05-870 Błonie, Radzików, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ślesak</LastName><ForeName>Ireneusz</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek Street 21, 30-001 Cracow, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dąbrowska-Bronk</LastName><ForeName>Joanna</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Karpiński</LastName><ForeName>Stanisław</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-776 Warszawa, Poland. Electronic address: stanislaw_karpinski@sggw.pl.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>04</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006706" MajorTopicYN="N">Homeostasis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006824" MajorTopicYN="N">Hybridization, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Development</Keyword><Keyword MajorTopicYN="N">Photosynthesis</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Salicylic acid</Keyword><Keyword MajorTopicYN="N">Senescence</Keyword><Keyword MajorTopicYN="N">Tree morphology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>5</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29730441</ArticleId><ArticleId IdType="pii">S0176-1617(18)30123-8</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2018.04.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Bernacki, Maciej Jerzy" sort="Bernacki, Maciej Jerzy" uniqKey="Bernacki M" first="Maciej Jerzy" last="Bernacki">Maciej Jerzy Bernacki</name></noRegion><name sortKey=" Lesak, Ireneusz" sort=" Lesak, Ireneusz" uniqKey=" Lesak I" first="Ireneusz" last=" Lesak">Ireneusz Lesak</name><name sortKey="Czarnocka, Weronika" sort="Czarnocka, Weronika" uniqKey="Czarnocka W" first="Weronika" last="Czarnocka">Weronika Czarnocka</name><name sortKey="D Browska Bronk, Joanna" sort="D Browska Bronk, Joanna" uniqKey="D Browska Bronk J" first="Joanna" last="D Browska-Bronk">Joanna D Browska-Bronk</name><name sortKey="Karpi Ski, Stanislaw" sort="Karpi Ski, Stanislaw" uniqKey="Karpi Ski S" first="Stanisław" last="Karpi Ski">Stanisław Karpi Ski</name><name sortKey="Rusaczonek, Anna" sort="Rusaczonek, Anna" uniqKey="Rusaczonek A" first="Anna" last="Rusaczonek">Anna Rusaczonek</name><name sortKey="Szechy Ska Hebda, Magdalena" sort="Szechy Ska Hebda, Magdalena" uniqKey="Szechy Ska Hebda M" first="Magdalena" last="Szechy Ska-Hebda">Magdalena Szechy Ska-Hebda</name><name sortKey="Wito, Damian" sort="Wito, Damian" uniqKey="Wito D" first="Damian" last="Wito">Damian Wito</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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