RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus.
Identifieur interne : 000D07 ( Main/Exploration ); précédent : 000D06; suivant : 000D08RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus.
Auteurs : Fouzia Tabssum [Pakistan] ; Muhammad Irfan ; Hafiz Abdullah Shakir [Pakistan] ; Javed Iqbal Qazi [Pakistan]Source :
- Journal of biological engineering [ 1754-1611 ] ; 2018.
Abstract
Background
Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of
Results
The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO
Conclusion
These results suggested potential utilization of this strain in biofuel industry.
DOI: 10.1186/s13036-018-0097-4
PubMed: 29755582
PubMed Central: PMC5934882
Affiliations:
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Pakistan.</nlm:affiliation><wicri:noCountry code="subField">Punjab Pakistan</wicri:noCountry></affiliation></author><author><name sortKey="Shakir, Hafiz Abdullah" sort="Shakir, Hafiz Abdullah" uniqKey="Shakir H" first="Hafiz Abdullah" last="Shakir">Hafiz Abdullah Shakir</name><affiliation wicri:level="1"><nlm:affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore</wicri:regionArea><wicri:noRegion>Lahore</wicri:noRegion></affiliation></author><author><name sortKey="Qazi, Javed Iqbal" sort="Qazi, Javed Iqbal" uniqKey="Qazi J" first="Javed Iqbal" last="Qazi">Javed Iqbal Qazi</name><affiliation wicri:level="1"><nlm:affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore</wicri:regionArea><wicri:noRegion>Lahore</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29755582</idno><idno type="pmid">29755582</idno><idno type="doi">10.1186/s13036-018-0097-4</idno><idno type="pmc">PMC5934882</idno><idno type="wicri:Area/Main/Corpus">000E37</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E37</idno><idno type="wicri:Area/Main/Curation">000E37</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E37</idno><idno type="wicri:Area/Main/Exploration">000E37</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RSM based optimization of nutritional conditions for cellulase mediated Saccharification by <i>Bacillus cereus</i>.</title><author><name sortKey="Tabssum, Fouzia" sort="Tabssum, Fouzia" uniqKey="Tabssum F" first="Fouzia" last="Tabssum">Fouzia Tabssum</name><affiliation wicri:level="1"><nlm:affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore</wicri:regionArea><wicri:noRegion>Lahore</wicri:noRegion></affiliation></author><author><name sortKey="Irfan, Muhammad" sort="Irfan, Muhammad" uniqKey="Irfan M" first="Muhammad" last="Irfan">Muhammad Irfan</name><affiliation><nlm:affiliation>2Department of Biotechnology, University of Sargodha, University road, Sargodha, Punjab Pakistan.</nlm:affiliation><wicri:noCountry code="subField">Punjab Pakistan</wicri:noCountry></affiliation></author><author><name sortKey="Shakir, Hafiz Abdullah" sort="Shakir, Hafiz Abdullah" uniqKey="Shakir H" first="Hafiz Abdullah" last="Shakir">Hafiz Abdullah Shakir</name><affiliation wicri:level="1"><nlm:affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore</wicri:regionArea><wicri:noRegion>Lahore</wicri:noRegion></affiliation></author><author><name sortKey="Qazi, Javed Iqbal" sort="Qazi, Javed Iqbal" uniqKey="Qazi J" first="Javed Iqbal" last="Qazi">Javed Iqbal Qazi</name><affiliation wicri:level="1"><nlm:affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore</wicri:regionArea><wicri:noRegion>Lahore</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biological engineering</title><idno type="ISSN">1754-1611</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO</p></div><div type="abstract" xml:lang="en"><p><b>Conclusion</b></p><p>These results suggested potential utilization of this strain in biofuel industry.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29755582</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-1611</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Journal of biological engineering</Title><ISOAbbreviation>J Biol Eng</ISOAbbreviation></Journal><ArticleTitle>RSM based optimization of nutritional conditions for cellulase mediated Saccharification by <i>Bacillus cereus</i>.</ArticleTitle><Pagination><MedlinePgn>7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13036-018-0097-4</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of <i>Bacillus cereus</i> was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, <i>Labeo rohita</i> and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimized through response surface methodology. Initially, Plackett-Burman design was used for screening purpose and optimization was conducted through Box-Bhenken design.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO<sub>4</sub>, 0.04% peptone, 2% poplar waste biomass, initial medium pH of 9.0, and inoculum size of 2% <i>v</i>/v at 37 °C with agitation speed of 120 rpm for 24 h of submerged fermentation. The proposed model for optimization of cellulase production was found highly significant. The indigenously produced cellulase enzyme was employed for saccharification purpose at 50 °C for various time periods. Maximum total sugars of 31.42 mg/ml were released after 6 h of incubation at 50 °C.The efficiency of this enzyme was compared with commercial cellulase enzyme revealing significant findings.</AbstractText><AbstractText Label="Conclusion" NlmCategory="UNASSIGNED">These results suggested potential utilization of this strain in biofuel industry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tabssum</LastName><ForeName>Fouzia</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</Affiliation><Identifier Source="ISNI">0000 0001 0670 519X</Identifier><Identifier Source="GRID">grid.11173.35</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Irfan</LastName><ForeName>Muhammad</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>2Department of Biotechnology, University of Sargodha, University road, Sargodha, Punjab Pakistan.</Affiliation><Identifier Source="ISNI">0000 0004 0609 4693</Identifier><Identifier Source="GRID">grid.412782.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shakir</LastName><ForeName>Hafiz Abdullah</ForeName><Initials>HA</Initials><AffiliationInfo><Affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</Affiliation><Identifier Source="ISNI">0000 0001 0670 519X</Identifier><Identifier Source="GRID">grid.11173.35</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qazi</LastName><ForeName>Javed Iqbal</ForeName><Initials>JI</Initials><AffiliationInfo><Affiliation>1Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore, Pakistan.</Affiliation><Identifier Source="ISNI">0000 0001 0670 519X</Identifier><Identifier Source="GRID">grid.11173.35</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Biol Eng</MedlineTA><NlmUniqueID>101306640</NlmUniqueID><ISSNLinking>1754-1611</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacillus sp. 16S rRNA</Keyword><Keyword MajorTopicYN="N">Cellulase</Keyword><Keyword MajorTopicYN="N">Labeo rohita</Keyword><Keyword MajorTopicYN="N">RSM</Keyword></KeywordList><CoiStatement>The data presented in this paper does not need any ethical approval.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29755582</ArticleId><ArticleId IdType="doi">10.1186/s13036-018-0097-4</ArticleId><ArticleId IdType="pii">97</ArticleId><ArticleId IdType="pmc">PMC5934882</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Curr Opin Microbiol. 2005 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Aug;18(5):355-83</Citation><ArticleIdList><ArticleId IdType="pubmed">14538100</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Pakistan</li></country></list><tree><noCountry><name sortKey="Irfan, Muhammad" sort="Irfan, Muhammad" uniqKey="Irfan M" first="Muhammad" last="Irfan">Muhammad Irfan</name></noCountry><country name="Pakistan"><noRegion><name sortKey="Tabssum, Fouzia" sort="Tabssum, Fouzia" uniqKey="Tabssum F" first="Fouzia" last="Tabssum">Fouzia Tabssum</name></noRegion><name sortKey="Qazi, Javed Iqbal" sort="Qazi, Javed Iqbal" uniqKey="Qazi J" first="Javed Iqbal" last="Qazi">Javed Iqbal Qazi</name><name sortKey="Shakir, Hafiz Abdullah" sort="Shakir, Hafiz Abdullah" uniqKey="Shakir H" first="Hafiz Abdullah" last="Shakir">Hafiz Abdullah Shakir</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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