Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).
Identifieur interne : 000A28 ( Main/Exploration ); précédent : 000A27; suivant : 000A29Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).
Auteurs : Karolina M. Czajka [Canada] ; Paul Michael [Canada] ; Kabwe Nkongolo [Canada]Source :
- Ecotoxicology (London, England) [ 1573-3017 ] ; 2019.
Descripteurs français
- KwdFr :
- Germination (effets des médicaments et des substances chimiques), Germination (génétique), Graines (croissance et développement), Nickel (MeSH), Polluants du sol (toxicité), Populus (génétique), Populus (physiologie), Protéines végétales (physiologie), Relation dose-effet des médicaments (MeSH), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques).
- MESH :
- croissance et développement : Graines.
- effets des médicaments et des substances chimiques : Germination, Régulation de l'expression des gènes végétaux.
- génétique : Germination, Populus.
- physiologie : Populus, Protéines végétales.
- toxicité : Polluants du sol.
- Nickel, Relation dose-effet des médicaments.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Plant Proteins.
- chemical , toxicity : Soil Pollutants.
- chemical : Nickel.
- drug effects : Gene Expression Regulation, Plant, Germination.
- genetics : Germination, Populus.
- growth & development : Seeds.
- physiology : Populus.
- Dose-Response Relationship, Drug.
Abstract
It has been demonstrated that a number of metals including mercury (Hg), zinc (Zn), cadmium (Cd), cobalt (Co), lead (Pb), copper (Cu), and nickel (Ni) decrease seed germination rates and plant growth. The threshold levels of metal toxicity on seed germination, plant development, and gene regulation have not been studied in detail. The main objective of this study was to assess in vitro and in vivo the effects of different doses of nickel on Trembling aspen (Populus tremuloides) seed germination and regulation of the high affinity nickel transporter family protein (AT2G16800) gene. The in vitro assays showed that Nickel completely inhibited seed germination even at the lowest concentration of 0.401 mg Ni per mL (in media) tested. However, when the same concentration of nickel (150 mg Ni per 1 kg of dry soil) was added to soil samples, during the vivo assays, almost all of the seeds germinated. Significant inhibition of seed germination was observed when soil samples were treated with at least 400 mg/kg of Ni. No damages were observed on growing seedlings treated with 150, 400, and 800 mg/kg of Ni. Only the highest dose of 1, 600 mg/kg resulted in visible leaf and stem damages and reduced growth on 75% of seedlings. A significant repression of the AT2G16800 gene was observed for the 400, 800, and 1600 mg/kg of nickel treatments compared to the water control with the lowest level of expression observed in samples treated with 800 mg/kg of Ni. Results of this study suggest that P. tremuloides populations will likely be sustainable for long term in sites that are highly contaminated with Ni including mining regions since the bioavailable amount of this metal is usually below 400 mg/kg in Canada.
DOI: 10.1007/s10646-018-2003-8
PubMed: 30552523
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).</title><author><name sortKey="Czajka, Karolina M" sort="Czajka, Karolina M" uniqKey="Czajka K" first="Karolina M" last="Czajka">Karolina M. Czajka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author><author><name sortKey="Michael, Paul" sort="Michael, Paul" uniqKey="Michael P" first="Paul" last="Michael">Paul Michael</name><affiliation wicri:level="1"><nlm:affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author><author><name sortKey="Nkongolo, Kabwe" sort="Nkongolo, Kabwe" uniqKey="Nkongolo K" first="Kabwe" last="Nkongolo">Kabwe Nkongolo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30552523</idno><idno type="pmid">30552523</idno><idno type="doi">10.1007/s10646-018-2003-8</idno><idno type="wicri:Area/Main/Corpus">000B23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B23</idno><idno type="wicri:Area/Main/Curation">000B23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B23</idno><idno type="wicri:Area/Main/Exploration">000B23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).</title><author><name sortKey="Czajka, Karolina M" sort="Czajka, Karolina M" uniqKey="Czajka K" first="Karolina M" last="Czajka">Karolina M. Czajka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author><author><name sortKey="Michael, Paul" sort="Michael, Paul" uniqKey="Michael P" first="Paul" last="Michael">Paul Michael</name><affiliation wicri:level="1"><nlm:affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author><author><name sortKey="Nkongolo, Kabwe" sort="Nkongolo, Kabwe" uniqKey="Nkongolo K" first="Kabwe" last="Nkongolo">Kabwe Nkongolo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6</wicri:regionArea><wicri:noRegion>P3E 2C6</wicri:noRegion></affiliation></author></analytic><series><title level="j">Ecotoxicology (London, England)</title><idno type="eISSN">1573-3017</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Dose-Response Relationship, Drug (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Germination (drug effects)</term><term>Germination (genetics)</term><term>Nickel (MeSH)</term><term>Plant Proteins (physiology)</term><term>Populus (genetics)</term><term>Populus (physiology)</term><term>Seeds (growth & development)</term><term>Soil Pollutants (toxicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Germination (effets des médicaments et des substances chimiques)</term><term>Germination (génétique)</term><term>Graines (croissance et développement)</term><term>Nickel (MeSH)</term><term>Polluants du sol (toxicité)</term><term>Populus (génétique)</term><term>Populus (physiologie)</term><term>Protéines végétales (physiologie)</term><term>Relation dose-effet des médicaments (MeSH)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Soil Pollutants</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Nickel</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Graines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Germination</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Germination</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Germination</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Germination</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Polluants du sol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Dose-Response Relationship, Drug</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Nickel</term><term>Relation dose-effet des médicaments</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It has been demonstrated that a number of metals including mercury (Hg), zinc (Zn), cadmium (Cd), cobalt (Co), lead (Pb), copper (Cu), and nickel (Ni) decrease seed germination rates and plant growth. The threshold levels of metal toxicity on seed germination, plant development, and gene regulation have not been studied in detail. The main objective of this study was to assess in vitro and in vivo the effects of different doses of nickel on Trembling aspen (Populus tremuloides) seed germination and regulation of the high affinity nickel transporter family protein (AT2G16800) gene. The in vitro assays showed that Nickel completely inhibited seed germination even at the lowest concentration of 0.401 mg Ni per mL (in media) tested. However, when the same concentration of nickel (150 mg Ni per 1 kg of dry soil) was added to soil samples, during the vivo assays, almost all of the seeds germinated. Significant inhibition of seed germination was observed when soil samples were treated with at least 400 mg/kg of Ni. No damages were observed on growing seedlings treated with 150, 400, and 800 mg/kg of Ni. Only the highest dose of 1, 600 mg/kg resulted in visible leaf and stem damages and reduced growth on 75% of seedlings. A significant repression of the AT2G16800 gene was observed for the 400, 800, and 1600 mg/kg of nickel treatments compared to the water control with the lowest level of expression observed in samples treated with 800 mg/kg of Ni. Results of this study suggest that P. tremuloides populations will likely be sustainable for long term in sites that are highly contaminated with Ni including mining regions since the bioavailable amount of this metal is usually below 400 mg/kg in Canada.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30552523</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-3017</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Ecotoxicology (London, England)</Title><ISOAbbreviation>Ecotoxicology</ISOAbbreviation></Journal><ArticleTitle>Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).</ArticleTitle><Pagination><MedlinePgn>92-102</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10646-018-2003-8</ELocationID><Abstract><AbstractText>It has been demonstrated that a number of metals including mercury (Hg), zinc (Zn), cadmium (Cd), cobalt (Co), lead (Pb), copper (Cu), and nickel (Ni) decrease seed germination rates and plant growth. The threshold levels of metal toxicity on seed germination, plant development, and gene regulation have not been studied in detail. The main objective of this study was to assess in vitro and in vivo the effects of different doses of nickel on Trembling aspen (Populus tremuloides) seed germination and regulation of the high affinity nickel transporter family protein (AT2G16800) gene. The in vitro assays showed that Nickel completely inhibited seed germination even at the lowest concentration of 0.401 mg Ni per mL (in media) tested. However, when the same concentration of nickel (150 mg Ni per 1 kg of dry soil) was added to soil samples, during the vivo assays, almost all of the seeds germinated. Significant inhibition of seed germination was observed when soil samples were treated with at least 400 mg/kg of Ni. No damages were observed on growing seedlings treated with 150, 400, and 800 mg/kg of Ni. Only the highest dose of 1, 600 mg/kg resulted in visible leaf and stem damages and reduced growth on 75% of seedlings. A significant repression of the AT2G16800 gene was observed for the 400, 800, and 1600 mg/kg of nickel treatments compared to the water control with the lowest level of expression observed in samples treated with 800 mg/kg of Ni. Results of this study suggest that P. tremuloides populations will likely be sustainable for long term in sites that are highly contaminated with Ni including mining regions since the bioavailable amount of this metal is usually below 400 mg/kg in Canada.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Czajka</LastName><ForeName>Karolina M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michael</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nkongolo</LastName><ForeName>Kabwe</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biology, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, P3E 2C6, Canada. knkongolo@laurentian.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>NSERC-CRD 47535-14</GrantID><Agency>Natural Sciences and Engineering Research Council of Canada</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Ecotoxicology</MedlineTA><NlmUniqueID>9885956</NlmUniqueID><ISSNLinking>0963-9292</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance UI="D009532">Nickel</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="N">Germination</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009532" MajorTopicYN="N">Nickel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AT2G16800 gene expression</Keyword><Keyword MajorTopicYN="N">Nickel toxicity</Keyword><Keyword MajorTopicYN="N">Populus tremuloides</Keyword><Keyword MajorTopicYN="N">Seed germination</Keyword><Keyword MajorTopicYN="N">Trembling aspen</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30552523</ArticleId><ArticleId IdType="doi">10.1007/s10646-018-2003-8</ArticleId><ArticleId IdType="pii">10.1007/s10646-018-2003-8</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Sci. 2000 Aug 8;157(1):113-128</Citation><ArticleIdList><ArticleId IdType="pubmed">10940475</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2001 Nov;52(364):2169-79</Citation><ArticleIdList><ArticleId IdType="pubmed">11604456</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Environ Contam Toxicol. 2002 Aug;43(2):203-13</Citation><ArticleIdList><ArticleId IdType="pubmed">12115046</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2002 Aug;115(4):504-512</Citation><ArticleIdList><ArticleId IdType="pubmed">12121456</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2003 Jun;44(6):649-52</Citation><ArticleIdList><ArticleId IdType="pubmed">12826631</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2004 Feb;120(2):249-255</Citation><ArticleIdList><ArticleId IdType="pubmed">15032859</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2005 Mar;95(4):609-18</Citation><ArticleIdList><ArticleId IdType="pubmed">15642725</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Med Chem. 2005;12(10):1161-208</Citation><ArticleIdList><ArticleId IdType="pubmed">15892631</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Technol. 2005 Aug 15;39(16):5927-32</Citation><ArticleIdList><ArticleId IdType="pubmed">16173548</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 2006 Jan;188(1):317-27</Citation><ArticleIdList><ArticleId IdType="pubmed">16352848</ArticleId></ArticleIdList></Reference><Reference><Citation>Bull Environ Contam Toxicol. 2007 May;78(5):319-24</Citation><ArticleIdList><ArticleId IdType="pubmed">17619800</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2009 Apr;103(6):923-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19201764</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecotoxicol Environ Saf. 2010 Sep;73(6):1123-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20138361</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2010 Mar 04;11:150</Citation><ArticleIdList><ArticleId IdType="pubmed">20199690</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2011 Apr;31(4):452-61</Citation><ArticleIdList><ArticleId IdType="pubmed">21427158</ArticleId></ArticleIdList></Reference><Reference><Citation>Rev Environ Contam Toxicol. 2011;213:113-36</Citation><ArticleIdList><ArticleId IdType="pubmed">21541849</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 2012 May;164:53-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22336730</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2013 Feb;130:69-74</Citation><ArticleIdList><ArticleId IdType="pubmed">23306112</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Geochem Health. 2015 Dec;37(6):1041-61</Citation><ArticleIdList><ArticleId IdType="pubmed">25841357</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Sep 17;10(9):e0138162</Citation><ArticleIdList><ArticleId IdType="pubmed">26378446</ArticleId></ArticleIdList></Reference><Reference><Citation>ScientificWorldJournal. 2015;2015:595073</Citation><ArticleIdList><ArticleId IdType="pubmed">26550604</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2016 Apr 15;11(4):e0153762</Citation><ArticleIdList><ArticleId IdType="pubmed">27082755</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 2017 Apr;223:497-506</Citation><ArticleIdList><ArticleId IdType="pubmed">28139323</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Genomics. 2018 Nov;40(11):1127-1136</Citation><ArticleIdList><ArticleId IdType="pubmed">30315518</ArticleId></ArticleIdList></Reference><Reference><Citation>Nutr Metab. 1979;23(1):62-4</Citation><ArticleIdList><ArticleId IdType="pubmed">310529</ArticleId></ArticleIdList></Reference><Reference><Citation>Bull Environ Contam Toxicol. 1994 Mar;52(3):452-6</Citation><ArticleIdList><ArticleId IdType="pubmed">8142719</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Czajka, Karolina M" sort="Czajka, Karolina M" uniqKey="Czajka K" first="Karolina M" last="Czajka">Karolina M. Czajka</name></noRegion><name sortKey="Michael, Paul" sort="Michael, Paul" uniqKey="Michael P" first="Paul" last="Michael">Paul Michael</name><name sortKey="Nkongolo, Kabwe" sort="Nkongolo, Kabwe" uniqKey="Nkongolo K" first="Kabwe" last="Nkongolo">Kabwe Nkongolo</name><name sortKey="Nkongolo, Kabwe" sort="Nkongolo, Kabwe" uniqKey="Nkongolo K" first="Kabwe" last="Nkongolo">Kabwe Nkongolo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A28 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000A28 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30552523
|texte= Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30552523" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |