PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.
Identifieur interne : 000168 ( Main/Exploration ); précédent : 000167; suivant : 000169PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.
Auteurs : Hailang Wang [République populaire de Chine] ; Chao Xu [République populaire de Chine] ; Youbing Zhang [République populaire de Chine] ; Xu Yan [République populaire de Chine] ; Xiaohuan Jin [République populaire de Chine] ; Xiaoqing Yao [République populaire de Chine] ; Peng Chen [République populaire de Chine] ; Bo Zheng [République populaire de Chine]Source :
- Tree physiology [ 1758-4469 ] ; 2020.
Abstract
The multi-subunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue-specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular ncm5U (5-carbamoylmethyluridine) and mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine). Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. In addition, the protein products of PtKTI12A and PtKTI12B had similar subcellular localisation and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar.
DOI: 10.1093/treephys/tpaa088
PubMed: 32705117
Affiliations:
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Ltd.,Changsha 410001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yuan Longping High-Tech Agriculture Co. Ltd.,Changsha 410001</wicri:regionArea><wicri:noRegion>Changsha 410001</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Youbing" sort="Zhang, Youbing" uniqKey="Zhang Y" first="Youbing" last="Zhang">Youbing Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Guangzhou Vipotion Biotechnology Co. Ltd., Guangzhou 510663, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou Vipotion Biotechnology Co. Ltd., Guangzhou 510663</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yan, Xu" sort="Yan, Xu" uniqKey="Yan X" first="Xu" last="Yan">Xu Yan</name><affiliation wicri:level="1"><nlm:affiliation>Shacheng Middle School, Zhangjiakou 075000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shacheng Middle School, Zhangjiakou 075000</wicri:regionArea><wicri:noRegion>Zhangjiakou 075000</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Xiaohuan" sort="Jin, Xiaohuan" uniqKey="Jin X" first="Xiaohuan" last="Jin">Xiaohuan Jin</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yao, Xiaoqing" sort="Yao, Xiaoqing" uniqKey="Yao X" first="Xiaoqing" last="Yao">Xiaoqing Yao</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Chen, Peng" sort="Chen, Peng" uniqKey="Chen P" first="Peng" last="Chen">Peng Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zheng, Bo" sort="Zheng, Bo" uniqKey="Zheng B" first="Bo" last="Zheng">Bo Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticultural and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticultural and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The multi-subunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue-specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular ncm5U (5-carbamoylmethyluridine) and mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine). Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. In addition, the protein products of PtKTI12A and PtKTI12B had similar subcellular localisation and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32705117</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Jul</Month><Day>24</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">tpaa088</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpaa088</ELocationID><Abstract><AbstractText>The multi-subunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue-specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular ncm5U (5-carbamoylmethyluridine) and mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine). Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. In addition, the protein products of PtKTI12A and PtKTI12B had similar subcellular localisation and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hailang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Yuan Longping High-Tech Agriculture Co. Ltd.,Changsha 410001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Youbing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Guangzhou Vipotion Biotechnology Co. Ltd., Guangzhou 510663, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Xu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Shacheng Middle School, Zhangjiakou 075000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Xiaohuan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Xiaoqing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticultural and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus
</Keyword><Keyword MajorTopicYN="N">KTI12</Keyword><Keyword MajorTopicYN="N">drought</Keyword><Keyword MajorTopicYN="N">ncm5U</Keyword><Keyword MajorTopicYN="N">tRNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32705117</ArticleId><ArticleId IdType="pii">5875775</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpaa088</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li><li>Hubei</li></region><settlement><li>Jiangmen</li><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Wang, Hailang" sort="Wang, Hailang" uniqKey="Wang H" first="Hailang" last="Wang">Hailang Wang</name></region><name sortKey="Chen, Peng" sort="Chen, Peng" uniqKey="Chen P" first="Peng" last="Chen">Peng Chen</name><name sortKey="Jin, Xiaohuan" sort="Jin, Xiaohuan" uniqKey="Jin X" first="Xiaohuan" last="Jin">Xiaohuan Jin</name><name sortKey="Xu, Chao" sort="Xu, Chao" uniqKey="Xu C" first="Chao" last="Xu">Chao Xu</name><name sortKey="Yan, Xu" sort="Yan, Xu" uniqKey="Yan X" first="Xu" last="Yan">Xu Yan</name><name sortKey="Yao, Xiaoqing" sort="Yao, Xiaoqing" uniqKey="Yao X" first="Xiaoqing" last="Yao">Xiaoqing Yao</name><name sortKey="Zhang, Youbing" sort="Zhang, Youbing" uniqKey="Zhang Y" first="Youbing" last="Zhang">Youbing Zhang</name><name sortKey="Zheng, Bo" sort="Zheng, Bo" uniqKey="Zheng B" first="Bo" last="Zheng">Bo Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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