Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar.
Identifieur interne : 000036 ( Main/Exploration ); précédent : 000035; suivant : 000037Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar.
Auteurs : Christopher T. Straub [États-Unis] ; Ryan G. Bing [États-Unis] ; Jack P. Wang [États-Unis] ; Vincent L. Chiang [États-Unis] ; Michael W W. Adams [États-Unis] ; Robert M. Kelly [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2020.
Abstract
Background
Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (
Results
Of the 17 transgenic poplar lines examined with
Conclusions
Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing
DOI: 10.1186/s13068-020-01675-2
PubMed: 32180826
PubMed Central: PMC7065347
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<front><div type="abstract" xml:lang="en"><p><b>Background</b>
</p>
<p>Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (</p>
</div>
<div type="abstract" xml:lang="en"><p><b>Results</b>
</p>
<p>Of the 17 transgenic poplar lines examined with </p>
</div>
<div type="abstract" xml:lang="en"><p><b>Conclusions</b>
</p>
<p>Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing </p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32180826</PMID>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>28</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN>
<JournalIssue CitedMedium="Print"><Volume>13</Volume>
<PubDate><Year>2020</Year>
</PubDate>
</JournalIssue>
<Title>Biotechnology for biofuels</Title>
<ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation>
</Journal>
<ArticleTitle>Use of the lignocellulose-degrading bacterium <i>Caldicellulosiruptor bescii</i>
to assess recalcitrance and conversion of wild-type and transgenic poplar.</ArticleTitle>
<Pagination><MedlinePgn>43</MedlinePgn>
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<ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-020-01675-2</ELocationID>
<Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (<i>Populus trichocarpa</i>
) utilizing a lignocellulose-degrading, metabolically engineered bacterium, <i>Caldicellulosiruptor bescii</i>
. The poplar lines were assessed by incubation with an engineered <i>C. bescii</i>
strain that solubilized and converted the hexose and pentose carbohydrates to ethanol and acetate. The resulting fermentation titer and biomass solubilization were then utilized as a measure of biomass recalcitrance and compared to data previously reported on the transgenic poplar samples.</AbstractText>
<AbstractText Label="Results" NlmCategory="UNASSIGNED">Of the 17 transgenic poplar lines examined with <i>C. bescii</i>
, a wide variation in solubilization and fermentation titer was observed. While the wild type poplar control demonstrated relatively high recalcitrance with a total solubilization of only 20% and a fermentation titer of 7.3 mM, the transgenic lines resulted in solubilization ranging from 15 to 79% and fermentation titers from 6.8 to 29.6 mM. Additionally, a strong inverse correlation (<i>R</i>
<sup>2</sup>
= 0.8) between conversion efficiency and lignin content was observed with lower lignin samples more easily converted and solubilized by <i>C. bescii</i>
.</AbstractText>
<AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing <i>C. bescii</i>
as a screening assay for recalcitrance, poplar lines with down-regulation of coumarate 3-hydroxylase 3 (C3H3) resulted in the highest degrees of solubilization and conversion by <i>C. bescii</i>
. One such line, with a growth phenotype similar to the wild-type, generated more than three times the fermentation products of the wild-type poplar control, suggesting that excellent digestibility can be achieved without compromising fitness of the tree.</AbstractText>
<CopyrightInformation>© The Author(s) 2020.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Straub</LastName>
<ForeName>Christopher T</ForeName>
<Initials>CT</Initials>
<AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation>
<Identifier Source="GRID">grid.40803.3f</Identifier>
<Identifier Source="ISNI">0000 0001 2173 6074</Identifier>
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<Author ValidYN="Y"><LastName>Bing</LastName>
<ForeName>Ryan G</ForeName>
<Initials>RG</Initials>
<AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation>
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<AffiliationInfo><Affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</Affiliation>
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<Identifier Source="ISNI">0000 0001 2173 6074</Identifier>
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<Author ValidYN="Y"><LastName>Chiang</LastName>
<ForeName>Vincent L</ForeName>
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<AffiliationInfo><Affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</Affiliation>
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<Author ValidYN="Y"><LastName>Adams</LastName>
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<Initials>MWW</Initials>
<AffiliationInfo><Affiliation>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation>
<Identifier Source="GRID">grid.213876.9</Identifier>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kelly</LastName>
<ForeName>Robert M</ForeName>
<Initials>RM</Initials>
<Identifier Source="ORCID">0000-0002-0639-3592</Identifier>
<AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation>
<Identifier Source="GRID">grid.40803.3f</Identifier>
<Identifier Source="ISNI">0000 0001 2173 6074</Identifier>
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</Author>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>03</Month>
<Day>11</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Biotechnol Biofuels</MedlineTA>
<NlmUniqueID>101316935</NlmUniqueID>
<ISSNLinking>1754-6834</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biofuel</Keyword>
<Keyword MajorTopicYN="N">Caldicellulosiruptor</Keyword>
<Keyword MajorTopicYN="N">Extreme thermophiles</Keyword>
<Keyword MajorTopicYN="N">Lignocellulose</Keyword>
<Keyword MajorTopicYN="N">Poplar</Keyword>
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<CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement>
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{{Explor lien |wiki= Bois |area= PoplarV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:32180826 |texte= Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar. }}
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