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Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips.

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Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips.

Auteurs : M. Lambardi ; A. Fabbri ; A. Caccavale

Source :

Plant cell reports [ 1432-203X ] ; 2000.

RBID : pubmed:30754897

Abstract

Shoot tips from in vitro-grown, cold-hardened stock plants of white poplar (Populus alba L.) were successfully cryopreserved at -196 °C by one-step vitrification. After preculturing at 5 °C for 2 days on hormone-free MS medium containing different sucrose concentrations, and loading for 20 min with 2 M glycerol and 0.4 M sucrose, shoot tips were treated with the PVS2 vitrification solution and plunged directly into liquid nitrogen. Best survival rate (90%) was obtained when shoot tips were precultured on 0.09 M sucrose, hormone-free MS medium, vitrified by exposure to PVS2 solution for 60 min at 0 °C and, following cryopreservation, rewarmed at 40 °C and washed in 1.2 M sucrose solution for 20 min. Regrowth was improved by plating shoot tips on a gelled MS medium containing 1.5 μM N⁶-benzyladenine plus 0.5 μM gibberellic acid, while shoot rooting was achieved on MS medium containing 3 μM indole-3-butyric acid. Following this procedure, almost 60% rooted shoots were obtained from cryopreserved shoot tips.

DOI: 10.1007/s002990050001
PubMed: 30754897

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M. Lambardi

<affiliation><nlm:affiliation>Istituto sulla Propagazione delle Specie Legnose, Consiglio Nazionale delle Ricerche, via Ponte di Formicola 76, 50018 Scandicci (Florence), Italy e-mail: lambardi@ipsl.fi.cnr.it, Fax: +39-055-755121, , , , , , IT.</nlm:affiliation>
<wicri:noCountry code="subField">IT</wicri:noCountry>
</affiliation>

A. Fabbri

<affiliation><nlm:affiliation>Dipartimento di Biologia Evolutiva e Funzionale, Università degli Studi, viale delle Scienze, 43100 Parma, Italy, , , , , , IT.</nlm:affiliation>
<wicri:noCountry code="subField">IT</wicri:noCountry>
</affiliation>

A. Caccavale

<affiliation><nlm:affiliation>Istituto sulla Propagazione delle Specie Legnose, Consiglio Nazionale delle Ricerche, via Ponte di Formicola 76, 50018 Scandicci (Florence), Italy e-mail: lambardi@ipsl.fi.cnr.it, Fax: +39-055-755121, , , , , , IT.</nlm:affiliation>
<wicri:noCountry code="subField">IT</wicri:noCountry>
</affiliation>

Le document en format XML

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<front><div type="abstract" xml:lang="en"> Shoot tips from in vitro-grown, cold-hardened stock plants of white poplar (Populus alba L.) were successfully cryopreserved at -196  °C by one-step vitrification. After preculturing at 5  °C for 2 days on hormone-free MS medium containing different sucrose concentrations, and loading for 20 min with 2 M glycerol and 0.4 M sucrose, shoot tips were treated with the PVS2 vitrification solution and plunged directly into liquid nitrogen. Best survival rate (90%) was obtained when shoot tips were precultured on 0.09 M sucrose, hormone-free MS medium, vitrified by exposure to PVS2 solution for 60 min at 0  °C and, following cryopreservation, rewarmed at 40  °C and washed in 1.2 M sucrose solution for 20 min. Regrowth was improved by plating shoot tips on a gelled MS medium containing 1.5 μM N<sup>6</sup>
-benzyladenine plus 0.5 μM gibberellic acid, while shoot rooting was achieved on MS medium containing 3 μM indole-3-butyric acid. Following this procedure, almost 60% rooted shoots were obtained from cryopreserved shoot tips.</div>
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<Abstract><AbstractText> Shoot tips from in vitro-grown, cold-hardened stock plants of white poplar (Populus alba L.) were successfully cryopreserved at -196  °C by one-step vitrification. After preculturing at 5  °C for 2 days on hormone-free MS medium containing different sucrose concentrations, and loading for 20 min with 2 M glycerol and 0.4 M sucrose, shoot tips were treated with the PVS2 vitrification solution and plunged directly into liquid nitrogen. Best survival rate (90%) was obtained when shoot tips were precultured on 0.09 M sucrose, hormone-free MS medium, vitrified by exposure to PVS2 solution for 60 min at 0  °C and, following cryopreservation, rewarmed at 40  °C and washed in 1.2 M sucrose solution for 20 min. Regrowth was improved by plating shoot tips on a gelled MS medium containing 1.5 μM N<sup>6</sup>
-benzyladenine plus 0.5 μM gibberellic acid, while shoot rooting was achieved on MS medium containing 3 μM indole-3-butyric acid. Following this procedure, almost 60% rooted shoots were obtained from cryopreserved shoot tips.</AbstractText>
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<Keyword MajorTopicYN="N">Key words Populus alba L.</Keyword>
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Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips.

Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips.

Source :

Abstract

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Curation

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