Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione.
Identifieur interne : 004459 ( Main/Curation ); précédent : 004458; suivant : 004460Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione.
Auteurs : Katia D'Ambrosio [France] ; Brice Kauffmann ; Nicolas Rouhier ; Ettore Benedetti ; Jean-Pierre Jacquot ; André Aubry ; Catherine CorbierSource :
- Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2003.
Descripteurs français
- KwdFr :
- Arbres (MeSH), Clonage moléculaire (MeSH), Cristallisation (MeSH), Cristallographie aux rayons X (MeSH), Glutarédoxines (MeSH), Glutathion (composition chimique), Mutagenèse dirigée (MeSH), Oxidoreductases (MeSH), Protéines (composition chimique), Sélénométhionine (composition chimique), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Glutathione, Proteins, Selenomethionine.
- chemical : Glutaredoxins, Oxidoreductases.
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Electrophoresis, Polyacrylamide Gel, Mutagenesis, Site-Directed, Trees.
Abstract
A monocysteinic mutant of poplar glutaredoxin (C30S) has been overproduced and purified. The protein has been crystallized in complex with glutathione using the hanging-drop vapour-diffusion technique in the presence of PEG 4000 as a precipitating agent. A native data set was collected at 1.55 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.7, b = 49.1, c = 104.8 A. Isomorphous crystals of a selenomethionine derivative were grown under the same conditions. Three data sets were collected at 1.73 A using the FIP synchrotron beamline at the ESRF. The positions of the Se atoms were determined and model rebuilding and refinement are in progress.
DOI: 10.1107/s0907444903006814
PubMed: 12777768
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pubmed:12777768Le document en format XML
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<author><name sortKey="Jacquot, Jean Pierre" sort="Jacquot, Jean Pierre" uniqKey="Jacquot J" first="Jean-Pierre" last="Jacquot">Jean-Pierre Jacquot</name>
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<term>Crystallization (MeSH)</term>
<term>Crystallography, X-Ray (MeSH)</term>
<term>Electrophoresis, Polyacrylamide Gel (MeSH)</term>
<term>Glutaredoxins (MeSH)</term>
<term>Glutathione (chemistry)</term>
<term>Mutagenesis, Site-Directed (MeSH)</term>
<term>Oxidoreductases (MeSH)</term>
<term>Proteins (chemistry)</term>
<term>Selenomethionine (chemistry)</term>
<term>Trees (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Cristallisation (MeSH)</term>
<term>Cristallographie aux rayons X (MeSH)</term>
<term>Glutarédoxines (MeSH)</term>
<term>Glutathion (composition chimique)</term>
<term>Mutagenèse dirigée (MeSH)</term>
<term>Oxidoreductases (MeSH)</term>
<term>Protéines (composition chimique)</term>
<term>Sélénométhionine (composition chimique)</term>
<term>Électrophorèse sur gel de polyacrylamide (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutathione</term>
<term>Proteins</term>
<term>Selenomethionine</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glutaredoxins</term>
<term>Oxidoreductases</term>
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<term>Protéines</term>
<term>Sélénométhionine</term>
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<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Mutagenesis, Site-Directed</term>
<term>Trees</term>
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<term>Clonage moléculaire</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Glutarédoxines</term>
<term>Mutagenèse dirigée</term>
<term>Oxidoreductases</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
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<front><div type="abstract" xml:lang="en">A monocysteinic mutant of poplar glutaredoxin (C30S) has been overproduced and purified. The protein has been crystallized in complex with glutathione using the hanging-drop vapour-diffusion technique in the presence of PEG 4000 as a precipitating agent. A native data set was collected at 1.55 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.7, b = 49.1, c = 104.8 A. Isomorphous crystals of a selenomethionine derivative were grown under the same conditions. Three data sets were collected at 1.73 A using the FIP synchrotron beamline at the ESRF. The positions of the Se atoms were determined and model rebuilding and refinement are in progress.</div>
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<Abstract><AbstractText>A monocysteinic mutant of poplar glutaredoxin (C30S) has been overproduced and purified. The protein has been crystallized in complex with glutathione using the hanging-drop vapour-diffusion technique in the presence of PEG 4000 as a precipitating agent. A native data set was collected at 1.55 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.7, b = 49.1, c = 104.8 A. Isomorphous crystals of a selenomethionine derivative were grown under the same conditions. Three data sets were collected at 1.73 A using the FIP synchrotron beamline at the ESRF. The positions of the Se atoms were determined and model rebuilding and refinement are in progress.</AbstractText>
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