The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.
Identifieur interne : 002F23 ( Main/Curation ); précédent : 002F22; suivant : 002F24The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.
Auteurs : G. Qi [République populaire de Chine] ; J. Lu ; P. Zhang ; J. Li ; F. Zhu ; J. Chen ; Y. Liu ; Z. Yu ; X. ZhaoSource :
- Journal of applied microbiology [ 1365-2672 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Bacillus thuringiensis (génétique), Bacillus thuringiensis (métabolisme), Clonage moléculaire (MeSH), Coléoptères (effets des médicaments et des substances chimiques), Coléoptères (microbiologie), Données de séquences moléculaires (MeSH), Endotoxines (génétique), Endotoxines (isolement et purification), Endotoxines (toxicité), Femelle (MeSH), Hémolysines (génétique), Hémolysines (isolement et purification), Hémolysines (toxicité), Lutte biologique contre les nuisibles (MeSH), Mâle (MeSH), Protéines bactériennes (génétique), Protéines bactériennes (isolement et purification), Protéines bactériennes (toxicité), Protéines recombinantes (génétique), Protéines recombinantes (toxicité), Séquence d'acides aminés (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Coléoptères.
- génétique : Bacillus thuringiensis, Endotoxines, Hémolysines, Protéines bactériennes, Protéines recombinantes.
- isolement et purification : Endotoxines, Hémolysines, Protéines bactériennes.
- microbiologie : Coléoptères.
- métabolisme : Bacillus thuringiensis.
- toxicité : Endotoxines, Hémolysines, Protéines bactériennes, Protéines recombinantes.
- Animaux, Clonage moléculaire, Données de séquences moléculaires, Femelle, Lutte biologique contre les nuisibles, Mâle, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Bacillus thuringiensis (genetics), Bacillus thuringiensis (metabolism), Bacterial Proteins (genetics), Bacterial Proteins (isolation & purification), Bacterial Proteins (toxicity), Cloning, Molecular (MeSH), Coleoptera (drug effects), Coleoptera (microbiology), Endotoxins (genetics), Endotoxins (isolation & purification), Endotoxins (toxicity), Female (MeSH), Hemolysin Proteins (genetics), Hemolysin Proteins (isolation & purification), Hemolysin Proteins (toxicity), Male (MeSH), Molecular Sequence Data (MeSH), Pest Control, Biological (MeSH), Recombinant Proteins (genetics), Recombinant Proteins (toxicity).
- MESH :
- chemical , genetics : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Recombinant Proteins.
- drug effects : Coleoptera.
- genetics : Bacillus thuringiensis.
- chemical , isolation & purification : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- metabolism : Bacillus thuringiensis.
- microbiology : Coleoptera.
- chemical , toxicity : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Recombinant Proteins.
- Amino Acid Sequence, Animals, Cloning, Molecular, Female, Male, Molecular Sequence Data, Pest Control, Biological.
Abstract
AIMS
Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.
METHODS AND RESULTS
A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.
CONCLUSIONS
The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.
SIGNIFICANCE AND IMPACT OF THE STUDY
It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.
DOI: 10.1111/j.1365-2672.2011.04974.x
PubMed: 21332894
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pubmed:21332894Le document en format XML
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<term>Bacillus thuringiensis (metabolism)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (isolation & purification)</term>
<term>Bacterial Proteins (toxicity)</term>
<term>Cloning, Molecular (MeSH)</term>
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<term>Coleoptera (microbiology)</term>
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<term>Endotoxins (isolation & purification)</term>
<term>Endotoxins (toxicity)</term>
<term>Female (MeSH)</term>
<term>Hemolysin Proteins (genetics)</term>
<term>Hemolysin Proteins (isolation & purification)</term>
<term>Hemolysin Proteins (toxicity)</term>
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<term>Bacillus thuringiensis (métabolisme)</term>
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<term>Coléoptères (microbiologie)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Endotoxines (génétique)</term>
<term>Endotoxines (isolement et purification)</term>
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<term>Hémolysines (isolement et purification)</term>
<term>Hémolysines (toxicité)</term>
<term>Lutte biologique contre les nuisibles (MeSH)</term>
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<term>Female</term>
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<term>Molecular Sequence Data</term>
<term>Pest Control, Biological</term>
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<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en"><p><b>AIMS</b>
</p>
<p>Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b>
</p>
<p>A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b>
</p>
<p>It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.</p>
</div>
</front>
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<Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.</AbstractText>
<AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.</AbstractText>
<AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.</AbstractText>
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