Herbivore-induced blueberry volatiles and intra-plant signaling.
Identifieur interne : 002B84 ( Main/Curation ); précédent : 002B83; suivant : 002B85Herbivore-induced blueberry volatiles and intra-plant signaling.
Auteurs : Cesar R. Rodriguez-Saona [États-Unis]Source :
- Journal of visualized experiments : JoVE [ 1940-087X ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Composés organiques volatils.
- composition chimique : Myrtillier.
- métabolisme : Composés organiques volatils, Myrtillier.
- Animaux, Papillons de nuit, Transduction du signal.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Volatile Organic Compounds.
- chemistry : Blueberry Plants.
- metabolism : Blueberry Plants, Volatile Organic Compounds.
- Animals, Moths, Signal Transduction.
Abstract
Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack. These HIPVs are mainly regulated by the defensive plant hormone jasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA). Over the past 3 decades researchers have documented that HIPVs can repel or attract herbivores, attract the natural enemies of herbivores, and in some cases they can induce or prime plant defenses prior to herbivore attack. In a recent paper, I reported that feeding by gypsy moth caterpillars, exogenous MeJA application, and mechanical damage induce the emissions of volatiles from blueberry plants, albeit differently. In addition, blueberry branches respond to HIPVs emitted from neighboring branches of the same plant by increasing the levels of JA and resistance to herbivores (i.e., direct plant defenses), and by priming volatile emissions (i.e., indirect plant defenses). Similar findings have been reported recently for sagebrush, poplar, and lima beans. Here, I describe a push-pull method for collecting blueberry volatiles induced by herbivore (gypsy moth) feeding, exogenous MeJA application, and mechanical damage. The volatile collection unit consists of a 4 L volatile collection chamber, a 2-piece guillotine, an air delivery system that purifies incoming air, and a vacuum system connected to a trap filled with Super-Q adsorbent to collect volatiles. Volatiles collected in Super-Q traps are eluted with dichloromethane and then separated and quantified using Gas Chromatography (GC). This volatile collection method was used in my study to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants. These methods are described here. Briefly, undamaged blueberry branches are exposed to HIPVs from neighboring branches within the same plant. Using the same techniques described above, volatiles emitted from branches after exposure to HIPVs are collected and analyzed.
DOI: 10.3791/3440
PubMed: 22214939
PubMed Central: PMC3369640
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Rodriguez-Saona</name><affiliation wicri:level="4"><nlm:affiliation>Department of Entomology, Rutgers University.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">New Brunswick (New Jersey)</settlement><region type="state">New Jersey</region></placeName><orgName type="university">Université Rutgers</orgName></affiliation></author></analytic><series><title level="j">Journal of visualized experiments : JoVE</title><idno type="eISSN">1940-087X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Blueberry Plants (chemistry)</term><term>Blueberry Plants (metabolism)</term><term>Moths (MeSH)</term><term>Signal Transduction (MeSH)</term><term>Volatile Organic Compounds (analysis)</term><term>Volatile Organic Compounds (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Composés organiques volatils (analyse)</term><term>Composés organiques volatils (métabolisme)</term><term>Myrtillier (composition chimique)</term><term>Myrtillier (métabolisme)</term><term>Papillons de nuit (MeSH)</term><term>Transduction du signal (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Volatile Organic Compounds</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Composés organiques volatils</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Blueberry Plants</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Myrtillier</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Blueberry Plants</term><term>Volatile Organic Compounds</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Composés organiques volatils</term><term>Myrtillier</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Moths</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Papillons de nuit</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack. These HIPVs are mainly regulated by the defensive plant hormone jasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA). Over the past 3 decades researchers have documented that HIPVs can repel or attract herbivores, attract the natural enemies of herbivores, and in some cases they can induce or prime plant defenses prior to herbivore attack. In a recent paper, I reported that feeding by gypsy moth caterpillars, exogenous MeJA application, and mechanical damage induce the emissions of volatiles from blueberry plants, albeit differently. In addition, blueberry branches respond to HIPVs emitted from neighboring branches of the same plant by increasing the levels of JA and resistance to herbivores (i.e., direct plant defenses), and by priming volatile emissions (i.e., indirect plant defenses). Similar findings have been reported recently for sagebrush, poplar, and lima beans. Here, I describe a push-pull method for collecting blueberry volatiles induced by herbivore (gypsy moth) feeding, exogenous MeJA application, and mechanical damage. The volatile collection unit consists of a 4 L volatile collection chamber, a 2-piece guillotine, an air delivery system that purifies incoming air, and a vacuum system connected to a trap filled with Super-Q adsorbent to collect volatiles. Volatiles collected in Super-Q traps are eluted with dichloromethane and then separated and quantified using Gas Chromatography (GC). This volatile collection method was used in my study to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants. These methods are described here. Briefly, undamaged blueberry branches are exposed to HIPVs from neighboring branches within the same plant. Using the same techniques described above, volatiles emitted from branches after exposure to HIPVs are collected and analyzed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22214939</PMID><DateCompleted><Year>2012</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1940-087X</ISSN><JournalIssue CitedMedium="Internet"><Issue>58</Issue><PubDate><Year>2011</Year><Month>Dec</Month><Day>18</Day></PubDate></JournalIssue><Title>Journal of visualized experiments : JoVE</Title><ISOAbbreviation>J Vis Exp</ISOAbbreviation></Journal><ArticleTitle>Herbivore-induced blueberry volatiles and intra-plant signaling.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3791/3440</ELocationID><ELocationID EIdType="pii" ValidYN="Y">3440</ELocationID><Abstract><AbstractText>Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack. These HIPVs are mainly regulated by the defensive plant hormone jasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA). Over the past 3 decades researchers have documented that HIPVs can repel or attract herbivores, attract the natural enemies of herbivores, and in some cases they can induce or prime plant defenses prior to herbivore attack. In a recent paper, I reported that feeding by gypsy moth caterpillars, exogenous MeJA application, and mechanical damage induce the emissions of volatiles from blueberry plants, albeit differently. In addition, blueberry branches respond to HIPVs emitted from neighboring branches of the same plant by increasing the levels of JA and resistance to herbivores (i.e., direct plant defenses), and by priming volatile emissions (i.e., indirect plant defenses). Similar findings have been reported recently for sagebrush, poplar, and lima beans. Here, I describe a push-pull method for collecting blueberry volatiles induced by herbivore (gypsy moth) feeding, exogenous MeJA application, and mechanical damage. The volatile collection unit consists of a 4 L volatile collection chamber, a 2-piece guillotine, an air delivery system that purifies incoming air, and a vacuum system connected to a trap filled with Super-Q adsorbent to collect volatiles. Volatiles collected in Super-Q traps are eluted with dichloromethane and then separated and quantified using Gas Chromatography (GC). This volatile collection method was used in my study to investigate the volatile response of undamaged branches to exposure to volatiles from herbivore-damaged branches within blueberry plants. These methods are described here. Briefly, undamaged blueberry branches are exposed to HIPVs from neighboring branches within the same plant. Using the same techniques described above, volatiles emitted from branches after exposure to HIPVs are collected and analyzed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rodriguez-Saona</LastName><ForeName>Cesar R</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>Department of Entomology, Rutgers University.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D059040">Video-Audio Media</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>12</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Vis Exp</MedlineTA><NlmUniqueID>101313252</NlmUniqueID><ISSNLinking>1940-087X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D055549">Volatile Organic Compounds</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029798" MajorTopicYN="N">Blueberry Plants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055549" MajorTopicYN="N">Volatile Organic Compounds</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22214939</ArticleId><ArticleId IdType="pii">3440</ArticleId><ArticleId IdType="doi">10.3791/3440</ArticleId><ArticleId IdType="pmc">PMC3369640</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 1990 Nov 30;250(4985):1251-3</Citation><ArticleIdList><ArticleId IdType="pubmed">17829213</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2007 Jun;10(6):490-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17498148</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5467-72</Citation><ArticleIdList><ArticleId IdType="pubmed">17360371</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 2009 Feb;35(2):163-75</Citation><ArticleIdList><ArticleId IdType="pubmed">19159981</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1994 Sep 26;352(2):146-50</Citation><ArticleIdList><ArticleId IdType="pubmed">7925964</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecology. 2006 Apr;87(4):922-30</Citation><ArticleIdList><ArticleId IdType="pubmed">16676536</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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