Genomic stability and long-term transgene expression in poplar.
Identifieur interne : 002571 ( Main/Curation ); précédent : 002570; suivant : 002572Genomic stability and long-term transgene expression in poplar.
Auteurs : Matthias Fladung [Allemagne] ; Hans Hoenicka ; M. Raj AhujaSource :
- Transgenic research [ 1573-9368 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Arbres (génétique), Cellules végétales (métabolisme), Instabilité du génome (MeSH), Populus (croissance et développement), Populus (génétique), Régulation de l'expression des gènes végétaux (MeSH), Techniques de culture cellulaire (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus.
- génétique : ADN bactérien, Arbres, Populus, Végétaux génétiquement modifiés.
- métabolisme : Cellules végétales, Végétaux génétiquement modifiés.
- Instabilité du génome, Régulation de l'expression des gènes végétaux, Techniques de culture cellulaire, Transgènes.
English descriptors
- KwdEn :
- Cell Culture Techniques (MeSH), DNA, Bacterial (genetics), Gene Expression Regulation, Plant (MeSH), Genomic Instability (MeSH), Plant Cells (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Transgenes (MeSH), Trees (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial.
- genetics : Plants, Genetically Modified, Populus, Trees.
- growth & development : Populus.
- metabolism : Plant Cells, Plants, Genetically Modified.
- Cell Culture Techniques, Gene Expression Regulation, Plant, Genomic Instability, Transgenes.
Abstract
Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. Both, the 19 years old tissue cultures and the up to 18 years old glasshouse-grown trees revealed expression of the rolC transgene, as demonstrated by the rolC-phenotype and/or northern blot experiments confirming long-term transgene expression.
DOI: 10.1007/s11248-013-9719-2
PubMed: 23740206
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Raj Ahuja</name></author></analytic><series><title level="j">Transgenic research</title><idno type="eISSN">1573-9368</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Culture Techniques (MeSH)</term><term>DNA, Bacterial (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genomic Instability (MeSH)</term><term>Plant Cells (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Transgenes (MeSH)</term><term>Trees (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Arbres (génétique)</term><term>Cellules végétales (métabolisme)</term><term>Instabilité du génome (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Techniques de culture cellulaire (MeSH)</term><term>Transgènes (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Arbres</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Cells</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Culture Techniques</term><term>Gene Expression Regulation, Plant</term><term>Genomic Instability</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Instabilité du génome</term><term>Régulation de l'expression des gènes végétaux</term><term>Techniques de culture cellulaire</term><term>Transgènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. Both, the 19 years old tissue cultures and the up to 18 years old glasshouse-grown trees revealed expression of the rolC transgene, as demonstrated by the rolC-phenotype and/or northern blot experiments confirming long-term transgene expression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23740206</PMID><DateCompleted><Year>2014</Year><Month>07</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-9368</ISSN><JournalIssue CitedMedium="Internet"><Volume>22</Volume><Issue>6</Issue><PubDate><Year>2013</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Genomic stability and long-term transgene expression in poplar.</ArticleTitle><Pagination><MedlinePgn>1167-78</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11248-013-9719-2</ELocationID><Abstract><AbstractText>Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. 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