Genomic stability and long-term transgene expression in poplar.
Identifieur interne : 002571 ( Main/Curation ); précédent : 002570; suivant : 002572Genomic stability and long-term transgene expression in poplar.
Auteurs : Matthias Fladung [Allemagne] ; Hans Hoenicka ; M. Raj AhujaSource :
- Transgenic research [ 1573-9368 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Arbres (génétique), Cellules végétales (métabolisme), Instabilité du génome (MeSH), Populus (croissance et développement), Populus (génétique), Régulation de l'expression des gènes végétaux (MeSH), Techniques de culture cellulaire (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus.
- génétique : ADN bactérien, Arbres, Populus, Végétaux génétiquement modifiés.
- métabolisme : Cellules végétales, Végétaux génétiquement modifiés.
- Instabilité du génome, Régulation de l'expression des gènes végétaux, Techniques de culture cellulaire, Transgènes.
English descriptors
- KwdEn :
- Cell Culture Techniques (MeSH), DNA, Bacterial (genetics), Gene Expression Regulation, Plant (MeSH), Genomic Instability (MeSH), Plant Cells (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Transgenes (MeSH), Trees (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial.
- genetics : Plants, Genetically Modified, Populus, Trees.
- growth & development : Populus.
- metabolism : Plant Cells, Plants, Genetically Modified.
- Cell Culture Techniques, Gene Expression Regulation, Plant, Genomic Instability, Transgenes.
Abstract
Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. Both, the 19 years old tissue cultures and the up to 18 years old glasshouse-grown trees revealed expression of the rolC transgene, as demonstrated by the rolC-phenotype and/or northern blot experiments confirming long-term transgene expression.
DOI: 10.1007/s11248-013-9719-2
PubMed: 23740206
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<term>Genomic Instability (MeSH)</term>
<term>Plant Cells (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (metabolism)</term>
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<term>Populus (croissance et développement)</term>
<term>Populus (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Techniques de culture cellulaire (MeSH)</term>
<term>Transgènes (MeSH)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. Both, the 19 years old tissue cultures and the up to 18 years old glasshouse-grown trees revealed expression of the rolC transgene, as demonstrated by the rolC-phenotype and/or northern blot experiments confirming long-term transgene expression.</div>
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<Abstract><AbstractText>Stable expression of foreign genes over the entire life span of a plant is important for long-lived organisms such as trees. For transgenic forest trees, very little information is available on long-term transgene expression and genomic stability. Independent transgenic lines obtained directly after transformation are initially screened in respect to T-DNA integration and transgene expression. However, very little consideration has been given to long-term transgene stability in long-lived forest trees. We have investigated possible genome wide changes following T-DNA integration as well as long-term stability of transgene expression in different transgenic lines of hybrid aspen (Populus tremula × Populus tremuloides) that are up to 19 years old. For studies on possible genome wide changes following T-DNA integration, four different independent rolC-transgenic lines were subjected to an extensive AFLP study and compared to the non-transgenic control line. Only minor genomic changes following T-DNA integration could be detected. To study long-term transgene expression, six different independent rolC-transgenic lines produced in 1993 and since that time have been kept continuously under in vitro conditions. In addition, 18 transgenic plants belonging to eight independent rolC-transgenic lines transferred to glasshouse between 1994 and 2004 were chosen to determine the presence and expression of the rolC gene. In all transgenic lines examined, the rolC gene could successfully be amplified by PCR tests. Both, the 19 years old tissue cultures and the up to 18 years old glasshouse-grown trees revealed expression of the rolC transgene, as demonstrated by the rolC-phenotype and/or northern blot experiments confirming long-term transgene expression.</AbstractText>
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<ReferenceList><Reference><Citation>J Biotechnol. 2007 Jan 1;127(2):310-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16889860</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2003 Jul;1(4):241-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17163901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2005 Jul;23(7):785; author reply 787-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16003352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetika. 2005 Dec;41(12):1601-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16396445</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1995 Nov 11;23(21):4407-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7501463</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2012 Nov;53(11):1974-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23026817</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2012 Jun;23(3):315-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22093808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2006 Sep;62(1-2):15-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16900326</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2010 Jul;28(7):656-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20622831</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2008 Apr 24;452(7190):991-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18432245</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2005 Jun;58(3):295-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16021396</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2002 Aug;31(4):543-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12182710</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Lett. 2010 Jan;32(1):151-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19731043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2011 Oct;234(4):699-707</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21617990</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2011 Aug;1809(8):360-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21549230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechniques. 1995 May;18(5):800-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7619481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1987 Dec 20;6(13):3891-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16453816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2009 Feb;29(2):299-312</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19203955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomed Biotechnol. 2006;2006(2):25376</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16883050</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Biotechnol. 2010 Jun 03;10:41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20525262</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2005 Jan;57(2):271-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15821882</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Theor Appl Genet. 1981 Oct;60(4):197-214</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24276737</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 1999 Jan;260(6):574-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9928937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2006;57(14):3737-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17032730</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2013;956:83-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23135846</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2001 Sep;213(5):731-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11678277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Biotechnol. 2012 Jul 15;12:41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22794278</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2011 Oct;30(10):1847-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21643815</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Rep. 2013 Apr;40(4):3281-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23283739</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 2000 Sep;264(1-2):20-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11016829</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2011 Feb;9(2):216-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20633239</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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