A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus.
Identifieur interne : 001887 ( Main/Curation ); précédent : 001886; suivant : 001888A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus.
Auteurs : Elena Fernández-Fueyo [Pays-Bas] ; Francisco J. Ruiz-Due As [Espagne] ; María F. L Pez-Lucendo [Espagne] ; Marta Pérez-Boada [Espagne] ; Jorge Rencoret [Espagne] ; Ana Gutiérrez [Espagne] ; Antonio G. Pisabarro [Espagne] ; Lucía Ramírez [Espagne] ; Angel T. Martínez [Espagne]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2016.
Abstract
BACKGROUND
Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose cultures.
RESULTS
Monokaryotic P. ostreatus (PC9) was grown with poplar wood or wheat straw as the sole C/N source and the extracellular proteins were analyzed, together with those from glucose medium. Using nano-liquid chromatography coupled to tandem mass spectrometry of whole-protein hydrolyzate, over five-hundred proteins were identified. Thirty-four percent were unique of the straw cultures, while only 15 and 6 % were unique of the glucose and poplar cultures, respectively (20 % were produced under the three conditions, and additional 19 % were shared by the two lignocellulose cultures). Semi-quantitative analysis showed oxidoreductases as the main protein type both in the poplar (39 % total abundance) and straw (31 %) secretomes, while carbohydrate-active enzymes (CAZys) were only slightly overproduced (14-16 %). Laccase 10 (LACC10) was the main protein in the two lignocellulose secretomes (10-14 %) and, together with LACC2, LACC9, LACC6, versatile peroxidase 1 (VP1), and manganese peroxidase 3 (MnP3), were strongly overproduced in the lignocellulose cultures. Seven CAZys were also among the top-50 proteins, but only CE16 acetylesterase was overproduced on lignocellulose. When the woody and nonwoody secretomes were compared, GH1 and GH3 β-glycosidases were more abundant on poplar and straw, respectively and, among less abundant proteins, VP2 was overproduced on straw, while VP3 was only found on poplar. The treated lignocellulosic substrates were analyzed by two-dimensional nuclear magnetic resonance (2D NMR), and a decrease of lignin relative to carbohydrate signals was observed, together with the disappearance of some minor lignin substructures, and an increase of sugar reducing ends.
CONCLUSIONS
Oxidoreductases are strongly induced when P. ostreatus grows on woody and nonwoody lignocellulosic substrates. One laccase occupied the first position in both secretomes, and three more were overproduced together with one VP and one MnP, suggesting an important role in lignocellulose degradation. Preferential removal of lignin vs carbohydrates was shown by 2D NMR, in agreement with the above secretomic results.
DOI: 10.1186/s13068-016-0462-9
PubMed: 26933449
PubMed Central: PMC4772462
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Ruiz-Due As</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="L Pez Lucendo, Maria F" sort="L Pez Lucendo, Maria F" uniqKey="L Pez Lucendo M" first="María F" last="L Pez-Lucendo">María F. L Pez-Lucendo</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Perez Boada, Marta" sort="Perez Boada, Marta" uniqKey="Perez Boada M" first="Marta" last="Pérez-Boada">Marta Pérez-Boada</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Rencoret, Jorge" sort="Rencoret, Jorge" uniqKey="Rencoret J" first="Jorge" last="Rencoret">Jorge Rencoret</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville</wicri:regionArea></affiliation></author><author><name sortKey="Gutierrez, Ana" sort="Gutierrez, Ana" uniqKey="Gutierrez A" first="Ana" last="Gutiérrez">Ana Gutiérrez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville</wicri:regionArea></affiliation></author><author><name sortKey="Pisabarro, Antonio G" sort="Pisabarro, Antonio G" uniqKey="Pisabarro A" first="Antonio G" last="Pisabarro">Antonio G. 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Ruiz-Due As</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="L Pez Lucendo, Maria F" sort="L Pez Lucendo, Maria F" uniqKey="L Pez Lucendo M" first="María F" last="L Pez-Lucendo">María F. L Pez-Lucendo</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Perez Boada, Marta" sort="Perez Boada, Marta" uniqKey="Perez Boada M" first="Marta" last="Pérez-Boada">Marta Pérez-Boada</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Rencoret, Jorge" sort="Rencoret, Jorge" uniqKey="Rencoret J" first="Jorge" last="Rencoret">Jorge Rencoret</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville</wicri:regionArea></affiliation></author><author><name sortKey="Gutierrez, Ana" sort="Gutierrez, Ana" uniqKey="Gutierrez A" first="Ana" last="Gutiérrez">Ana Gutiérrez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville</wicri:regionArea></affiliation></author><author><name sortKey="Pisabarro, Antonio G" sort="Pisabarro, Antonio G" uniqKey="Pisabarro A" first="Antonio G" last="Pisabarro">Antonio G. Pisabarro</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona</wicri:regionArea></affiliation></author><author><name sortKey="Ramirez, Lucia" sort="Ramirez, Lucia" uniqKey="Ramirez L" first="Lucía" last="Ramírez">Lucía Ramírez</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona</wicri:regionArea></affiliation></author><author><name sortKey="Martinez, Angel T" sort="Martinez, Angel T" uniqKey="Martinez A" first="Angel T" last="Martínez">Angel T. Martínez</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose cultures.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Monokaryotic P. ostreatus (PC9) was grown with poplar wood or wheat straw as the sole C/N source and the extracellular proteins were analyzed, together with those from glucose medium. Using nano-liquid chromatography coupled to tandem mass spectrometry of whole-protein hydrolyzate, over five-hundred proteins were identified. Thirty-four percent were unique of the straw cultures, while only 15 and 6 % were unique of the glucose and poplar cultures, respectively (20 % were produced under the three conditions, and additional 19 % were shared by the two lignocellulose cultures). Semi-quantitative analysis showed oxidoreductases as the main protein type both in the poplar (39 % total abundance) and straw (31 %) secretomes, while carbohydrate-active enzymes (CAZys) were only slightly overproduced (14-16 %). Laccase 10 (LACC10) was the main protein in the two lignocellulose secretomes (10-14 %) and, together with LACC2, LACC9, LACC6, versatile peroxidase 1 (VP1), and manganese peroxidase 3 (MnP3), were strongly overproduced in the lignocellulose cultures. Seven CAZys were also among the top-50 proteins, but only CE16 acetylesterase was overproduced on lignocellulose. When the woody and nonwoody secretomes were compared, GH1 and GH3 β-glycosidases were more abundant on poplar and straw, respectively and, among less abundant proteins, VP2 was overproduced on straw, while VP3 was only found on poplar. The treated lignocellulosic substrates were analyzed by two-dimensional nuclear magnetic resonance (2D NMR), and a decrease of lignin relative to carbohydrate signals was observed, together with the disappearance of some minor lignin substructures, and an increase of sugar reducing ends.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Oxidoreductases are strongly induced when P. ostreatus grows on woody and nonwoody lignocellulosic substrates. One laccase occupied the first position in both secretomes, and three more were overproduced together with one VP and one MnP, suggesting an important role in lignocellulose degradation. Preferential removal of lignin vs carbohydrates was shown by 2D NMR, in agreement with the above secretomic results.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26933449</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus.</ArticleTitle><Pagination><MedlinePgn>49</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-016-0462-9</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose cultures.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Monokaryotic P. ostreatus (PC9) was grown with poplar wood or wheat straw as the sole C/N source and the extracellular proteins were analyzed, together with those from glucose medium. Using nano-liquid chromatography coupled to tandem mass spectrometry of whole-protein hydrolyzate, over five-hundred proteins were identified. Thirty-four percent were unique of the straw cultures, while only 15 and 6 % were unique of the glucose and poplar cultures, respectively (20 % were produced under the three conditions, and additional 19 % were shared by the two lignocellulose cultures). Semi-quantitative analysis showed oxidoreductases as the main protein type both in the poplar (39 % total abundance) and straw (31 %) secretomes, while carbohydrate-active enzymes (CAZys) were only slightly overproduced (14-16 %). Laccase 10 (LACC10) was the main protein in the two lignocellulose secretomes (10-14 %) and, together with LACC2, LACC9, LACC6, versatile peroxidase 1 (VP1), and manganese peroxidase 3 (MnP3), were strongly overproduced in the lignocellulose cultures. Seven CAZys were also among the top-50 proteins, but only CE16 acetylesterase was overproduced on lignocellulose. When the woody and nonwoody secretomes were compared, GH1 and GH3 β-glycosidases were more abundant on poplar and straw, respectively and, among less abundant proteins, VP2 was overproduced on straw, while VP3 was only found on poplar. The treated lignocellulosic substrates were analyzed by two-dimensional nuclear magnetic resonance (2D NMR), and a decrease of lignin relative to carbohydrate signals was observed, together with the disappearance of some minor lignin substructures, and an increase of sugar reducing ends.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Oxidoreductases are strongly induced when P. ostreatus grows on woody and nonwoody lignocellulosic substrates. One laccase occupied the first position in both secretomes, and three more were overproduced together with one VP and one MnP, suggesting an important role in lignocellulose degradation. Preferential removal of lignin vs carbohydrates was shown by 2D NMR, in agreement with the above secretomic results.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fernández-Fueyo</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruiz-Dueñas</LastName><ForeName>Francisco J</ForeName><Initials>FJ</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Lucendo</LastName><ForeName>María F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pérez-Boada</LastName><ForeName>Marta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rencoret</LastName><ForeName>Jorge</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gutiérrez</LastName><ForeName>Ana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, PO Box 1052, 41080 Seville, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pisabarro</LastName><ForeName>Antonio G</ForeName><Initials>AG</Initials><AffiliationInfo><Affiliation>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ramírez</LastName><ForeName>Lucía</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Agrarian Production, Universidad Pública de Navarra, 31006, Pamplona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martínez</LastName><ForeName>Angel T</ForeName><Initials>AT</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2D NMR</Keyword><Keyword MajorTopicYN="N">Carbohydrate-active enzymes</Keyword><Keyword MajorTopicYN="N">LC–MS/MS</Keyword><Keyword MajorTopicYN="N">Laccases</Keyword><Keyword MajorTopicYN="N">Lignin-modifying enzymes</Keyword><Keyword MajorTopicYN="N">Pleurotus ostreatus</Keyword><Keyword MajorTopicYN="N">Poplar wood</Keyword><Keyword MajorTopicYN="N">Secreted proteins</Keyword><Keyword MajorTopicYN="N">Wheat straw</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26933449</ArticleId><ArticleId IdType="doi">10.1186/s13068-016-0462-9</ArticleId><ArticleId IdType="pii">462</ArticleId><ArticleId IdType="pmc">PMC4772462</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mycologia. 2013 Nov-Dec;105(6):1428-44</Citation><ArticleIdList><ArticleId IdType="pubmed">23921235</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2010 May;86(6):1903-14</Citation><ArticleIdList><ArticleId IdType="pubmed">20306191</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 2005 Jan;47(1):49-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15551134</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Biol (Noisy-le-grand). 2014 Dec 24;60(5):41-5</Citation><ArticleIdList><ArticleId IdType="pubmed">25535711</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Aug 5;333(6043):762-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21764756</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2014 Apr;80(7):2062-70</Citation><ArticleIdList><ArticleId IdType="pubmed">24441164</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1954-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19193860</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3253-65</Citation><ArticleIdList><ArticleId IdType="pubmed">25478843</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2010 Feb;85(5):1321-37</Citation><ArticleIdList><ArticleId IdType="pubmed">19956947</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2009 Jun;20(3):348-57</Citation><ArticleIdList><ArticleId IdType="pubmed">19502047</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 2000 Apr 1;376(1):171-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10729203</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2012 Feb 16;75(5):1493-504</Citation><ArticleIdList><ArticleId IdType="pubmed">22146477</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2011 Dec 21;75(2):642-54</Citation><ArticleIdList><ArticleId IdType="pubmed">21945728</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Jun;22(6):695-700</Citation><ArticleIdList><ArticleId IdType="pubmed">15122302</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1994 Nov 14;354(3):297-300</Citation><ArticleIdList><ArticleId IdType="pubmed">7957943</ArticleId></ArticleIdList></Reference><Reference><Citation>C R Biol. 2011 Nov;334(11):795-805</Citation><ArticleIdList><ArticleId IdType="pubmed">22078736</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2015 Aug 01;8:107</Citation><ArticleIdList><ArticleId IdType="pubmed">26236396</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:82-90</Citation><ArticleIdList><ArticleId IdType="pubmed">24853079</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2013 Jun;55:22-31</Citation><ArticleIdList><ArticleId IdType="pubmed">23583597</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1995 Jun;61(6):2408-13</Citation><ArticleIdList><ArticleId IdType="pubmed">7793961</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2011 Aug;321(1):14-23</Citation><ArticleIdList><ArticleId IdType="pubmed">21569082</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2005 Dec;5(18):4832-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16281184</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Technol. 2008 Sep 1;42(17):6703-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18800552</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2015 Nov;99(21):8927-42</Citation><ArticleIdList><ArticleId IdType="pubmed">25967658</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Feb;155(2):667-82</Citation><ArticleIdList><ArticleId IdType="pubmed">21098672</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 2015 Nov-Dec;107(6):1105-19</Citation><ArticleIdList><ArticleId IdType="pubmed">26297778</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2012 Feb;93(4):1395-410</Citation><ArticleIdList><ArticleId IdType="pubmed">22249717</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Rev. 2011 Jul 13;111(7):4111-40</Citation><ArticleIdList><ArticleId IdType="pubmed">21526768</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:124-30</Citation><ArticleIdList><ArticleId IdType="pubmed">24915038</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2011 Sep 29;8(10):785-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2007 Sep;153(Pt 9):3023-33</Citation><ArticleIdList><ArticleId IdType="pubmed">17768245</ArticleId></ArticleIdList></Reference><Reference><Citation>Int Microbiol. 2005 Sep;8(3):195-204</Citation><ArticleIdList><ArticleId IdType="pubmed">16200498</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2006 May;43(5):343-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16524749</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Adv. 2010 Nov-Dec;28(6):694-705</Citation><ArticleIdList><ArticleId IdType="pubmed">20471466</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2013 Apr;133:142-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23425584</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2015 Sep 18;290(38):23201-13</Citation><ArticleIdList><ArticleId IdType="pubmed">26240145</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2008 Sep;80(4):719-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18654772</ArticleId></ArticleIdList></Reference><Reference><Citation>ACS Chem Biol. 2011 Dec 16;6(12):1399-406</Citation><ArticleIdList><ArticleId IdType="pubmed">22004347</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2011 Nov;77(22):7933-41</Citation><ArticleIdList><ArticleId IdType="pubmed">21948841</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2014 Jun 18;15:486</Citation><ArticleIdList><ArticleId IdType="pubmed">24942338</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2014 Jan 03;7(1):2</Citation><ArticleIdList><ArticleId IdType="pubmed">24387130</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2004 Aug;65(3):287-94</Citation><ArticleIdList><ArticleId IdType="pubmed">14767623</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Jan 27;311(5760):484-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16439654</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2012 Jan;40(Database issue):D26-32</Citation><ArticleIdList><ArticleId IdType="pubmed">22110030</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2012 Jun;78(11):4037-45</Citation><ArticleIdList><ArticleId IdType="pubmed">22467498</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 2013 Nov-Dec;105(6):1412-27</Citation><ArticleIdList><ArticleId IdType="pubmed">23935027</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2009 Jun;75(12):4058-68</Citation><ArticleIdList><ArticleId IdType="pubmed">19376920</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1999 Aug;65(8):3413-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10427028</ArticleId></ArticleIdList></Reference><Reference><Citation>Microb Biotechnol. 2009 Mar;2(2):164-77</Citation><ArticleIdList><ArticleId IdType="pubmed">21261911</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2012 Jun 13;60(23):5922-35</Citation><ArticleIdList><ArticleId IdType="pubmed">22607527</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2010 Jun;76(11):3599-610</Citation><ArticleIdList><ArticleId IdType="pubmed">20400566</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:150-61</Citation><ArticleIdList><ArticleId IdType="pubmed">24560615</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2013 Mar 21;6(1):41</Citation><ArticleIdList><ArticleId IdType="pubmed">23514094</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11889-94</Citation><ArticleIdList><ArticleId IdType="pubmed">20547848</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Biol. 2015 Dec;119(12):1354-63</Citation><ArticleIdList><ArticleId IdType="pubmed">26615756</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2011 Jul;77(13):4499-507</Citation><ArticleIdList><ArticleId IdType="pubmed">21551287</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2011 Aug;102(16):7500-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21646018</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2013 Aug 10;6(1):115</Citation><ArticleIdList><ArticleId IdType="pubmed">23937687</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2014 Dec 04;10(12):e1004759</Citation><ArticleIdList><ArticleId IdType="pubmed">25474575</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Apr;167(4):1284-95</Citation><ArticleIdList><ArticleId IdType="pubmed">25667313</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1999 Aug 1;341 ( Pt 3):655-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10417329</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2007 Feb;44(2):77-87</Citation><ArticleIdList><ArticleId IdType="pubmed">16971147</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2015 Mar;76:78-92</Citation><ArticleIdList><ArticleId IdType="pubmed">25683379</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2014 Sep;80(18):5828-35</Citation><ArticleIdList><ArticleId IdType="pubmed">25015893</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 Jun 29;336(6089):1715-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22745431</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:168-81</Citation><ArticleIdList><ArticleId IdType="pubmed">25173823</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Biol (Noisy-le-grand). 2014 Dec 24;60(5):29-34</Citation><ArticleIdList><ArticleId IdType="pubmed">25535709</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2012 May 11;287(20):16903-16</Citation><ArticleIdList><ArticleId IdType="pubmed">22437835</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2008 Sep;80(3):381-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18636256</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2014 May 6;102:28-43</Citation><ArticleIdList><ArticleId IdType="pubmed">24631824</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2012 Dec 21;77:298-309</Citation><ArticleIdList><ArticleId IdType="pubmed">23000217</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Mar 6;452(7183):88-92</Citation><ArticleIdList><ArticleId IdType="pubmed">18322534</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytochemistry. 2008 Nov;69(16):2831-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18945458</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17501-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23045686</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2009 Sep;298(2):131-42</Citation><ArticleIdList><ArticleId IdType="pubmed">19622072</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2007 Jul;75(6):1293-300</Citation><ArticleIdList><ArticleId IdType="pubmed">17429621</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9923-8</Citation><ArticleIdList><ArticleId IdType="pubmed">24958869</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2005 Jul 21;118(1):17-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15888348</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5458-63</Citation><ArticleIdList><ArticleId IdType="pubmed">22434909</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2014 Jan 08;7(1):6</Citation><ArticleIdList><ArticleId IdType="pubmed">24401177</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2012 Sep;119:114-22</Citation><ArticleIdList><ArticleId IdType="pubmed">22728191</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2012 Sep;7(9):1579-89</Citation><ArticleIdList><ArticleId IdType="pubmed">22864199</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Biol (Noisy-le-grand). 2014 Dec 24;60(5):59-63</Citation><ArticleIdList><ArticleId IdType="pubmed">25535714</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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