Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa.
Identifieur interne : 001463 ( Main/Curation ); précédent : 001462; suivant : 001464Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa.
Auteurs : Stefania De Marco [Italie] ; Miranda Piccioni [Italie] ; Rita Pagiotti [Italie] ; Donatella Pietrella [Italie]Source :
- Evidence-based complementary and alternative medicine : eCAM [ 1741-427X ] ; 2017.
Abstract
Pseudomonas aeruginosa is a common biofilm-forming bacterial pathogen implicated in lung, skin, and systemic infections. Biofilms are majorly associated with chronic lung infection, which is the most severe complication in cystic fibrosis patients characterized by drug-resistant biofilms in the bronchial mucus with zones, where reactive oxygen species concentration is increased mainly due to neutrophil activity. Aim of this work is to verify the anti-Pseudomonas property of propolis or bud poplar resins extracts. The antimicrobial activity of propolis and bud poplar resins extracts was determined by MIC and biofilm quantification. Moreover, we tested the antioxidant activity by DPPH and neutrophil oxidative burst assays. In the end, both propolis and bud poplar resins extracts were able to inhibit P. aeruginosa biofilm formation and to influence both swimming and swarming motility. Moreover, the extracts could inhibit proinflammatory cytokine production by human PBMC and showed both direct and indirect antioxidant activity. This work is the first to demonstrate that propolis and bud poplar resins extracts can influence biofilm formation of P. aeruginosa contrasting the inflammation and the oxidation state typical of chronic infection suggesting that propolis or bud poplar resins can be used along with antibiotic as adjuvant in the therapy against P. aeruginosa infections related to biofilm.
DOI: 10.1155/2017/5163575
PubMed: 28127379
PubMed Central: PMC5239991
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Piccioni</name><affiliation wicri:level="1"><nlm:affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia</wicri:regionArea></affiliation></author><author><name sortKey="Pagiotti, Rita" sort="Pagiotti, Rita" uniqKey="Pagiotti R" first="Rita" last="Pagiotti">Rita Pagiotti</name><affiliation wicri:level="1"><nlm:affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia</wicri:regionArea></affiliation></author><author><name sortKey="Pietrella, Donatella" sort="Pietrella, Donatella" uniqKey="Pietrella D" first="Donatella" last="Pietrella">Donatella Pietrella</name><affiliation wicri:level="1"><nlm:affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia</wicri:regionArea></affiliation></author></analytic><series><title level="j">Evidence-based complementary and alternative medicine : eCAM</title><idno type="ISSN">1741-427X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Pseudomonas aeruginosa</i> is a common biofilm-forming bacterial pathogen implicated in lung, skin, and systemic infections. Biofilms are majorly associated with chronic lung infection, which is the most severe complication in cystic fibrosis patients characterized by drug-resistant biofilms in the bronchial mucus with zones, where reactive oxygen species concentration is increased mainly due to neutrophil activity. Aim of this work is to verify the anti-<i>Pseudomonas</i> property of propolis or bud poplar resins extracts. The antimicrobial activity of propolis and bud poplar resins extracts was determined by MIC and biofilm quantification. Moreover, we tested the antioxidant activity by DPPH and neutrophil oxidative burst assays. In the end, both propolis and bud poplar resins extracts were able to inhibit <i>P. aeruginosa</i> biofilm formation and to influence both swimming and swarming motility. Moreover, the extracts could inhibit proinflammatory cytokine production by human PBMC and showed both direct and indirect antioxidant activity. This work is the first to demonstrate that propolis and bud poplar resins extracts can influence biofilm formation of <i>P. aeruginosa</i> contrasting the inflammation and the oxidation state typical of chronic infection suggesting that propolis or bud poplar resins can be used along with antibiotic as adjuvant in the therapy against <i>P. aeruginosa</i> infections related to biofilm.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28127379</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1741-427X</ISSN><JournalIssue CitedMedium="Print"><Volume>2017</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Evidence-based complementary and alternative medicine : eCAM</Title><ISOAbbreviation>Evid Based Complement Alternat Med</ISOAbbreviation></Journal><ArticleTitle>Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus <i>Pseudomonas aeruginosa</i>.</ArticleTitle><Pagination><MedlinePgn>5163575</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2017/5163575</ELocationID><Abstract><AbstractText><i>Pseudomonas aeruginosa</i> is a common biofilm-forming bacterial pathogen implicated in lung, skin, and systemic infections. Biofilms are majorly associated with chronic lung infection, which is the most severe complication in cystic fibrosis patients characterized by drug-resistant biofilms in the bronchial mucus with zones, where reactive oxygen species concentration is increased mainly due to neutrophil activity. Aim of this work is to verify the anti-<i>Pseudomonas</i> property of propolis or bud poplar resins extracts. The antimicrobial activity of propolis and bud poplar resins extracts was determined by MIC and biofilm quantification. Moreover, we tested the antioxidant activity by DPPH and neutrophil oxidative burst assays. In the end, both propolis and bud poplar resins extracts were able to inhibit <i>P. aeruginosa</i> biofilm formation and to influence both swimming and swarming motility. Moreover, the extracts could inhibit proinflammatory cytokine production by human PBMC and showed both direct and indirect antioxidant activity. This work is the first to demonstrate that propolis and bud poplar resins extracts can influence biofilm formation of <i>P. aeruginosa</i> contrasting the inflammation and the oxidation state typical of chronic infection suggesting that propolis or bud poplar resins can be used along with antibiotic as adjuvant in the therapy against <i>P. aeruginosa</i> infections related to biofilm.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Marco</LastName><ForeName>Stefania</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Piccioni</LastName><ForeName>Miranda</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pagiotti</LastName><ForeName>Rita</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pietrella</LastName><ForeName>Donatella</ForeName><Initials>D</Initials><Identifier Source="ORCID">0000-0003-1572-4290</Identifier><AffiliationInfo><Affiliation>Biochemical Sciences and Health Section, Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Evid Based Complement Alternat Med</MedlineTA><NlmUniqueID>101215021</NlmUniqueID><ISSNLinking>1741-427X</ISSNLinking></MedlineJournalInfo><CoiStatement>The authors declare no competing financial interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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