An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).
Identifieur interne : 004884 ( Main/Corpus ); précédent : 004883; suivant : 004885An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).
Auteurs : K-H Han ; R. Meilan ; C. Ma ; S H StraussSource :
- Plant cell reports [ 1432-203X ] ; 2000.
Abstract
We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
DOI: 10.1007/s002990050019
PubMed: 30754915
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).</title><author><name sortKey="Han, K H" sort="Han, K H" uniqKey="Han K" first="K-H" last="Han">K-H Han</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Meilan, R" sort="Meilan, R" uniqKey="Meilan R" first="R" last="Meilan">R. Meilan</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, C" sort="Ma, C" uniqKey="Ma C" first="C" last="Ma">C. Ma</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Strauss, S H" sort="Strauss, S H" uniqKey="Strauss S" first="S H" last="Strauss">S H Strauss</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:30754915</idno><idno type="pmid">30754915</idno><idno type="doi">10.1007/s002990050019</idno><idno type="wicri:Area/Main/Corpus">004884</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004884</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).</title><author><name sortKey="Han, K H" sort="Han, K H" uniqKey="Han K" first="K-H" last="Han">K-H Han</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Meilan, R" sort="Meilan, R" uniqKey="Meilan R" first="R" last="Meilan">R. Meilan</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, C" sort="Ma, C" uniqKey="Ma C" first="C" last="Ma">C. Ma</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author><author><name sortKey="Strauss, S H" sort="Strauss, S H" uniqKey="Strauss S" first="S H" last="Strauss">S H Strauss</name><affiliation><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30754915</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>3</Issue><PubDate><Year>2000</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).</ArticleTitle><Pagination><MedlinePgn>315-320</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s002990050019</ELocationID><Abstract><AbstractText> We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Han</LastName><ForeName>K-H</ForeName><Initials>KH</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meilan</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>S H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-7501, USA e-mail: strauss@FSL.orst.edu Fax: +541-737-1393, , , , , , US.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium</Keyword><Keyword MajorTopicYN="N">Aspen</Keyword><Keyword MajorTopicYN="N">Cottonwood</Keyword><Keyword MajorTopicYN="N">Key words Poplar</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">Transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30754915</ArticleId><ArticleId IdType="doi">10.1007/s002990050019</ArticleId><ArticleId IdType="pii">10.1007/s002990050019</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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