Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.
Identifieur interne : 004819 ( Main/Corpus ); précédent : 004818; suivant : 004820Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.
Auteurs : B. Vinocur ; T. Carmi ; A. Altman ; M. ZivSource :
- Plant cell reports [ 1432-203X ] ; 2000.
Abstract
The regeneration potential of excised aspen (Populus tremula L.) roots cultivated in liquid medium, as affected by plant growth regulators and by the position of the isolated root explant on the main root, was investigated. The effect of various levels of benzyladenine (BA) and thidiazuron (TDZ) on bud regeneration in root explants was studied. TDZ in the medium had a marked effect on bud development as compared with BA, inducing a tenfold increase in the number of buds regenerated from various root explants. TDZ enhanced both root and root-borne shoot biomass production but reduced further shoot development and elongation. The position of the isolated root sections on the main root affected regeneration, the proximal sections further away from the root tip producing the highest number of buds per explant in both BA and TDZ treatments. Buds regenerated in close proximity to the site of lateral roots in BA-treated roots, while in TDZ-treated root sections, the buds formed all over the root regardless of the presence of lateral roots. The buds developed from inner cortical and sub-epidermal cell layers, disrupting the epidermis and the inner layers. Root biomass production and growth was greatly enhanced in well-aerated bioreactor culture in the presence of 4.5×10-2 μM TDZ. A high number of the root-borne shoots could be rooted and converted to plantlets. However, while shoots regenerated in a medium with BA rooted well in a growth regulator-free medium, shoots formed in a medium with TDZ required auxin for rooting. Roots cultured in the presence of ancymidol, a gibberellin biosynthesis inhibitor, regenerated non-hyperhydric bud clusters and hyperhydric shoots. These were separated mechanically, subcultured to growth and rooting medium and transplanted ex vitro resulting in phenotypically true-to-type plantlets. The potential of liquid cultures for aspen shoot biomass production from roots is discussed.
DOI: 10.1007/s002990000243
PubMed: 30754848
Links to Exploration step
pubmed:30754848Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.</title><author><name sortKey="Vinocur, B" sort="Vinocur, B" uniqKey="Vinocur B" first="B" last="Vinocur">B. Vinocur</name><affiliation><nlm:affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Carmi, T" sort="Carmi, T" uniqKey="Carmi T" first="T" last="Carmi">T. Carmi</name><affiliation><nlm:affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Altman, A" sort="Altman, A" uniqKey="Altman A" first="A" last="Altman">A. Altman</name><affiliation><nlm:affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Ziv, M" sort="Ziv, M" uniqKey="Ziv M" first="M" last="Ziv">M. Ziv</name><affiliation><nlm:affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:30754848</idno><idno type="pmid">30754848</idno><idno type="doi">10.1007/s002990000243</idno><idno type="wicri:Area/Main/Corpus">004819</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004819</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.</title><author><name sortKey="Vinocur, B" sort="Vinocur, B" uniqKey="Vinocur B" first="B" last="Vinocur">B. Vinocur</name><affiliation><nlm:affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Carmi, T" sort="Carmi, T" uniqKey="Carmi T" first="T" last="Carmi">T. Carmi</name><affiliation><nlm:affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Altman, A" sort="Altman, A" uniqKey="Altman A" first="A" last="Altman">A. Altman</name><affiliation><nlm:affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</nlm:affiliation></affiliation></author><author><name sortKey="Ziv, M" sort="Ziv, M" uniqKey="Ziv M" first="M" last="Ziv">M. Ziv</name><affiliation><nlm:affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> The regeneration potential of excised aspen (Populus tremula L.) roots cultivated in liquid medium, as affected by plant growth regulators and by the position of the isolated root explant on the main root, was investigated. The effect of various levels of benzyladenine (BA) and thidiazuron (TDZ) on bud regeneration in root explants was studied. TDZ in the medium had a marked effect on bud development as compared with BA, inducing a tenfold increase in the number of buds regenerated from various root explants. TDZ enhanced both root and root-borne shoot biomass production but reduced further shoot development and elongation. The position of the isolated root sections on the main root affected regeneration, the proximal sections further away from the root tip producing the highest number of buds per explant in both BA and TDZ treatments. Buds regenerated in close proximity to the site of lateral roots in BA-treated roots, while in TDZ-treated root sections, the buds formed all over the root regardless of the presence of lateral roots. The buds developed from inner cortical and sub-epidermal cell layers, disrupting the epidermis and the inner layers. Root biomass production and growth was greatly enhanced in well-aerated bioreactor culture in the presence of 4.5×10<sup>-2</sup> μM TDZ. A high number of the root-borne shoots could be rooted and converted to plantlets. However, while shoots regenerated in a medium with BA rooted well in a growth regulator-free medium, shoots formed in a medium with TDZ required auxin for rooting. Roots cultured in the presence of ancymidol, a gibberellin biosynthesis inhibitor, regenerated non-hyperhydric bud clusters and hyperhydric shoots. These were separated mechanically, subcultured to growth and rooting medium and transplanted ex vitro resulting in phenotypically true-to-type plantlets. The potential of liquid cultures for aspen shoot biomass production from roots is discussed.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30754848</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>12</Issue><PubDate><Year>2000</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.</ArticleTitle><Pagination><MedlinePgn>1146-1154</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s002990000243</ELocationID><Abstract><AbstractText> The regeneration potential of excised aspen (Populus tremula L.) roots cultivated in liquid medium, as affected by plant growth regulators and by the position of the isolated root explant on the main root, was investigated. The effect of various levels of benzyladenine (BA) and thidiazuron (TDZ) on bud regeneration in root explants was studied. TDZ in the medium had a marked effect on bud development as compared with BA, inducing a tenfold increase in the number of buds regenerated from various root explants. TDZ enhanced both root and root-borne shoot biomass production but reduced further shoot development and elongation. The position of the isolated root sections on the main root affected regeneration, the proximal sections further away from the root tip producing the highest number of buds per explant in both BA and TDZ treatments. Buds regenerated in close proximity to the site of lateral roots in BA-treated roots, while in TDZ-treated root sections, the buds formed all over the root regardless of the presence of lateral roots. The buds developed from inner cortical and sub-epidermal cell layers, disrupting the epidermis and the inner layers. Root biomass production and growth was greatly enhanced in well-aerated bioreactor culture in the presence of 4.5×10<sup>-2</sup> μM TDZ. A high number of the root-borne shoots could be rooted and converted to plantlets. However, while shoots regenerated in a medium with BA rooted well in a growth regulator-free medium, shoots formed in a medium with TDZ required auxin for rooting. Roots cultured in the presence of ancymidol, a gibberellin biosynthesis inhibitor, regenerated non-hyperhydric bud clusters and hyperhydric shoots. These were separated mechanically, subcultured to growth and rooting medium and transplanted ex vitro resulting in phenotypically true-to-type plantlets. The potential of liquid cultures for aspen shoot biomass production from roots is discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vinocur</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carmi</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Altman</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Kennedy-Leigh Center for Horticultural Research, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel, , , , , , IL.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ziv</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Agricultural Botany, Otto Warburg Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O Box 12, Rehovot 76100, Israel e-mail: meira@agri.huji.ac.il Fax: +972-8-9467763, , , , , , IL.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AbbreviationsANC: Ancymidol</Keyword><Keyword MajorTopicYN="N">BA: 6-Benzyladenine</Keyword><Keyword MajorTopicYN="N">DPB: Disposable plastic bioreactor</Keyword><Keyword MajorTopicYN="N">GA: Gibberellic acid</Keyword><Keyword MajorTopicYN="N">GV: Growth value</Keyword><Keyword MajorTopicYN="N">IAA: Indole-3-acetic acid</Keyword><Keyword MajorTopicYN="N">Keywords Populus tremula</Keyword><Keyword MajorTopicYN="N">Liquid culture</Keyword><Keyword MajorTopicYN="N">MSAL: Liquid medium for aspen culture</Keyword><Keyword MajorTopicYN="N">MSAS: Semi-solid medium</Keyword><Keyword MajorTopicYN="N">NAA : α-Naphtalene acetic acid</Keyword><Keyword MajorTopicYN="N">Root explants</Keyword><Keyword MajorTopicYN="N">TDZ: Thidiazuron</Keyword><Keyword MajorTopicYN="N">Thidiazuron</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30754848</ArticleId><ArticleId IdType="doi">10.1007/s002990000243</ArticleId><ArticleId IdType="pii">10.1007/s002990000243</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004819 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 004819 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:30754848
|texte= Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:30754848" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |