Colonisation of poplar trees by gfp expressing bacterial endophytes.
Identifieur interne : 004309 ( Main/Corpus ); précédent : 004308; suivant : 004310Colonisation of poplar trees by gfp expressing bacterial endophytes.
Auteurs : Kieran Germaine ; Elaine Keogh ; Guiomar Garcia-Cabellos ; Brigitte Borremans ; Daniel Lelie ; Tanja Barac ; Licy Oeyen ; Jaco Vangronsveld ; Fiona Porteous Moore ; Edward R B. Moore ; Colin D. Campbell ; David Ryan ; David N. DowlingSource :
- FEMS microbiology ecology [ 1574-6941 ] ; 2004.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (genetics), Bacteria (growth & development), Bacteria (isolation & purification), Biodiversity (MeSH), Colony Count, Microbial (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Genes, Reporter (MeSH), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Kanamycin Resistance (MeSH), Molecular Sequence Data (MeSH), Plant Leaves (microbiology), Plant Roots (microbiology), Populus (microbiology), RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH), Staining and Labeling (methods), Xylem (microbiology).
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Ribosomal.
- classification : Bacteria.
- genetics : Bacteria, DNA, Bacterial, DNA, Ribosomal, Green Fluorescent Proteins, RNA, Ribosomal, 16S.
- growth & development : Bacteria.
- isolation & purification : Bacteria.
- chemical , metabolism : Green Fluorescent Proteins.
- methods : Staining and Labeling.
- microbiology : Plant Leaves, Plant Roots, Populus, Xylem.
- Biodiversity, Colony Count, Microbial, Genes, Reporter, Kanamycin Resistance, Molecular Sequence Data, Sequence Analysis, DNA, Soil Microbiology.
Abstract
With the exception of nitrogen fixing bacteria, there is little known about the colonisation patterns or population sizes of bacterial endophytes in deciduous trees. This study describes the isolation, identification, construction and re-colonisation patterns of three green fluorescent protein(gfp):kanamycin(R) labelled bacterial endophytes when re-introduced into poplar trees, their original host plant. Two of these endophytes showed considerable colonisation in the roots and stems of inoculated plants. gfp expressing cells of all three strains were observed to colonise the xylem tissue of the root. All three strains proved to be efficient rhizosphere colonisers, supporting the theory that the rhizosphere can serve as a source of bacterial endophytes.
DOI: 10.1016/j.femsec.2003.12.009
PubMed: 19712436
Links to Exploration step
pubmed:19712436Le document en format XML
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Moore</name></author><author><name sortKey="Campbell, Colin D" sort="Campbell, Colin D" uniqKey="Campbell C" first="Colin D" last="Campbell">Colin D. Campbell</name></author><author><name sortKey="Ryan, David" sort="Ryan, David" uniqKey="Ryan D" first="David" last="Ryan">David Ryan</name></author><author><name sortKey="Dowling, David N" sort="Dowling, David N" uniqKey="Dowling D" first="David N" last="Dowling">David N. Dowling</name></author></analytic><series><title level="j">FEMS microbiology ecology</title><idno type="eISSN">1574-6941</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (classification)</term><term>Bacteria (genetics)</term><term>Bacteria (growth & development)</term><term>Bacteria (isolation & purification)</term><term>Biodiversity (MeSH)</term><term>Colony Count, Microbial (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Genes, Reporter (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Kanamycin Resistance (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Leaves (microbiology)</term><term>Plant Roots (microbiology)</term><term>Populus (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Staining and Labeling (methods)</term><term>Xylem (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>Green Fluorescent Proteins</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Leaves</term><term>Plant Roots</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Colony Count, Microbial</term><term>Genes, Reporter</term><term>Kanamycin Resistance</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the exception of nitrogen fixing bacteria, there is little known about the colonisation patterns or population sizes of bacterial endophytes in deciduous trees. This study describes the isolation, identification, construction and re-colonisation patterns of three green fluorescent protein(gfp):kanamycin(R) labelled bacterial endophytes when re-introduced into poplar trees, their original host plant. Two of these endophytes showed considerable colonisation in the roots and stems of inoculated plants. gfp expressing cells of all three strains were observed to colonise the xylem tissue of the root. All three strains proved to be efficient rhizosphere colonisers, supporting the theory that the rhizosphere can serve as a source of bacterial endophytes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19712436</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2009</Year><Month>08</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1574-6941</ISSN><JournalIssue CitedMedium="Internet"><Volume>48</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>FEMS microbiology ecology</Title><ISOAbbreviation>FEMS Microbiol Ecol</ISOAbbreviation></Journal><ArticleTitle>Colonisation of poplar trees by gfp expressing bacterial endophytes.</ArticleTitle><Pagination><MedlinePgn>109-18</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.femsec.2003.12.009</ELocationID><Abstract><AbstractText>With the exception of nitrogen fixing bacteria, there is little known about the colonisation patterns or population sizes of bacterial endophytes in deciduous trees. This study describes the isolation, identification, construction and re-colonisation patterns of three green fluorescent protein(gfp):kanamycin(R) labelled bacterial endophytes when re-introduced into poplar trees, their original host plant. Two of these endophytes showed considerable colonisation in the roots and stems of inoculated plants. gfp expressing cells of all three strains were observed to colonise the xylem tissue of the root. All three strains proved to be efficient rhizosphere colonisers, supporting the theory that the rhizosphere can serve as a source of bacterial endophytes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Germaine</LastName><ForeName>Kieran</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Applied Biology and Chemistry, Institute of Technology, Carlow, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Keogh</LastName><ForeName>Elaine</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Garcia-Cabellos</LastName><ForeName>Guiomar</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Borremans</LastName><ForeName>Brigitte</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Lelie</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Barac</LastName><ForeName>Tanja</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Oeyen</LastName><ForeName>Licy</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Vangronsveld</LastName><ForeName>Jaco</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Moore</LastName><ForeName>Fiona Porteous</ForeName><Initials>FP</Initials></Author><Author ValidYN="Y"><LastName>Moore</LastName><ForeName>Edward R B</ForeName><Initials>ER</Initials></Author><Author ValidYN="Y"><LastName>Campbell</LastName><ForeName>Colin D</ForeName><Initials>CD</Initials></Author><Author ValidYN="Y"><LastName>Ryan</LastName><ForeName>David</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Dowling</LastName><ForeName>David N</ForeName><Initials>DN</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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