Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya.
Identifieur interne : 003F71 ( Main/Corpus ); précédent : 003F70; suivant : 003F72Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya.
Auteurs : Qingyi Yu ; Paul H. Moore ; Henrik H. Albert ; Adrienne Hk Roader ; Ray MingSource :
- Cell research [ 1001-0602 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis Proteins (chemistry), Carica (genetics), Cloning, Molecular (MeSH), Evolution, Molecular (MeSH), Flowers (genetics), Flowers (growth & development), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Genome, Plant (MeSH), In Situ Hybridization (MeSH), MADS Domain Proteins (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), RNA, Messenger (genetics), RNA, Messenger (metabolism), Restriction Mapping (MeSH), Sequence Homology, Amino Acid (MeSH), Sex Characteristics (MeSH), Transcription Factors (chemistry).
- MESH :
- chemical , chemistry : Arabidopsis Proteins, Plant Proteins, Transcription Factors.
- genetics : Carica, Flowers, Plant Proteins, RNA, Messenger.
- growth & development : Flowers.
- chemical , metabolism : RNA, Messenger.
- Amino Acid Sequence, Cloning, Molecular, Evolution, Molecular, Gene Expression Profiling, Gene Expression Regulation, Plant, Genome, Plant, In Situ Hybridization, MADS Domain Proteins, Molecular Sequence Data, Phylogeny, Restriction Mapping, Sequence Homology, Amino Acid, Sex Characteristics.
Abstract
The homologous genes Floricaula (FLO) in Antirrhinum and LEAFY (LFY) in Arabidopsis are known to regulate the initiation of flowering in these two distantly related plant species. These genes are necessary also for the expression of downstream genes that control floral organ identity. We used Arabidopsis LFY cDNA as a probe to clone and sequence a papaya ortholog of LFY, PFL. It encodes a protein that shares 61% identity with the Arabidopsis LFY gene and 71% identity with the LFY homologs of the two woody tree species: California sycamore (Platanus racemosa) and black cottonwood (Populus trichocarpa). Despite the high sequence similarity within two conserved regions, the N-terminal proline-rich motif in papaya PFL differs from other members in the family. This difference may not affect the gene function of papaya PFL, since an equally divergent but a functional LFY ortholog Needly of Pinus radiata has been reported. Genomic and BAC Southern analyses indicated that there is only one copy of PFL in the papaya genome. In situ hybridization experiments demonstrated that PFL is expressed at a relatively low level in leaf primordia, but it is expressed at a high level in the floral meristem. Quantitative PCR analyses revealed that PFL was expressed in flower buds of all three sex types - male, female, and hermaphrodite with marginal difference between hermaphrodite and unisexual flowers. These data suggest that PFL may play a similar role as LFY in flower development and has limited effect on sex differentiation in papaya.
DOI: 10.1038/sj.cr.7290327
PubMed: 16117847
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pubmed:16117847Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya.</title><author><name sortKey="Yu, Qingyi" sort="Yu, Qingyi" uniqKey="Yu Q" first="Qingyi" last="Yu">Qingyi Yu</name><affiliation><nlm:affiliation>Hawaii Agriculture Research Center, Aiea, HI 96701, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Moore, Paul H" sort="Moore, Paul H" uniqKey="Moore P" first="Paul H" last="Moore">Paul H. Moore</name></author><author><name sortKey="Albert, Henrik H" sort="Albert, Henrik H" uniqKey="Albert H" first="Henrik H" last="Albert">Henrik H. Albert</name></author><author><name sortKey="Roader, Adrienne Hk" sort="Roader, Adrienne Hk" uniqKey="Roader A" first="Adrienne Hk" last="Roader">Adrienne Hk Roader</name></author><author><name sortKey="Ming, Ray" sort="Ming, Ray" uniqKey="Ming R" first="Ray" last="Ming">Ray Ming</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16117847</idno><idno type="pmid">16117847</idno><idno type="doi">10.1038/sj.cr.7290327</idno><idno type="wicri:Area/Main/Corpus">003F71</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003F71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya.</title><author><name sortKey="Yu, Qingyi" sort="Yu, Qingyi" uniqKey="Yu Q" first="Qingyi" last="Yu">Qingyi Yu</name><affiliation><nlm:affiliation>Hawaii Agriculture Research Center, Aiea, HI 96701, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Moore, Paul H" sort="Moore, Paul H" uniqKey="Moore P" first="Paul H" last="Moore">Paul H. Moore</name></author><author><name sortKey="Albert, Henrik H" sort="Albert, Henrik H" uniqKey="Albert H" first="Henrik H" last="Albert">Henrik H. Albert</name></author><author><name sortKey="Roader, Adrienne Hk" sort="Roader, Adrienne Hk" uniqKey="Roader A" first="Adrienne Hk" last="Roader">Adrienne Hk Roader</name></author><author><name sortKey="Ming, Ray" sort="Ming, Ray" uniqKey="Ming R" first="Ray" last="Ming">Ray Ming</name></author></analytic><series><title level="j">Cell research</title><idno type="ISSN">1001-0602</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Arabidopsis Proteins (chemistry)</term><term>Carica (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>Evolution, Molecular (MeSH)</term><term>Flowers (genetics)</term><term>Flowers (growth & development)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genome, Plant (MeSH)</term><term>In Situ Hybridization (MeSH)</term><term>MADS Domain Proteins (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Restriction Mapping (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Sex Characteristics (MeSH)</term><term>Transcription Factors (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arabidopsis Proteins</term><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carica</term><term>Flowers</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Flowers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Evolution, Molecular</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genome, Plant</term><term>In Situ Hybridization</term><term>MADS Domain Proteins</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Restriction Mapping</term><term>Sequence Homology, Amino Acid</term><term>Sex Characteristics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The homologous genes Floricaula (FLO) in Antirrhinum and LEAFY (LFY) in Arabidopsis are known to regulate the initiation of flowering in these two distantly related plant species. These genes are necessary also for the expression of downstream genes that control floral organ identity. We used Arabidopsis LFY cDNA as a probe to clone and sequence a papaya ortholog of LFY, PFL. It encodes a protein that shares 61% identity with the Arabidopsis LFY gene and 71% identity with the LFY homologs of the two woody tree species: California sycamore (Platanus racemosa) and black cottonwood (Populus trichocarpa). Despite the high sequence similarity within two conserved regions, the N-terminal proline-rich motif in papaya PFL differs from other members in the family. This difference may not affect the gene function of papaya PFL, since an equally divergent but a functional LFY ortholog Needly of Pinus radiata has been reported. Genomic and BAC Southern analyses indicated that there is only one copy of PFL in the papaya genome. In situ hybridization experiments demonstrated that PFL is expressed at a relatively low level in leaf primordia, but it is expressed at a high level in the floral meristem. Quantitative PCR analyses revealed that PFL was expressed in flower buds of all three sex types - male, female, and hermaphrodite with marginal difference between hermaphrodite and unisexual flowers. These data suggest that PFL may play a similar role as LFY in flower development and has limited effect on sex differentiation in papaya.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16117847</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>28</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1001-0602</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>8</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Cell research</Title><ISOAbbreviation>Cell Res</ISOAbbreviation></Journal><ArticleTitle>Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya.</ArticleTitle><Pagination><MedlinePgn>576-84</MedlinePgn></Pagination><Abstract><AbstractText>The homologous genes Floricaula (FLO) in Antirrhinum and LEAFY (LFY) in Arabidopsis are known to regulate the initiation of flowering in these two distantly related plant species. These genes are necessary also for the expression of downstream genes that control floral organ identity. We used Arabidopsis LFY cDNA as a probe to clone and sequence a papaya ortholog of LFY, PFL. It encodes a protein that shares 61% identity with the Arabidopsis LFY gene and 71% identity with the LFY homologs of the two woody tree species: California sycamore (Platanus racemosa) and black cottonwood (Populus trichocarpa). Despite the high sequence similarity within two conserved regions, the N-terminal proline-rich motif in papaya PFL differs from other members in the family. This difference may not affect the gene function of papaya PFL, since an equally divergent but a functional LFY ortholog Needly of Pinus radiata has been reported. Genomic and BAC Southern analyses indicated that there is only one copy of PFL in the papaya genome. In situ hybridization experiments demonstrated that PFL is expressed at a relatively low level in leaf primordia, but it is expressed at a high level in the floral meristem. Quantitative PCR analyses revealed that PFL was expressed in flower buds of all three sex types - male, female, and hermaphrodite with marginal difference between hermaphrodite and unisexual flowers. These data suggest that PFL may play a similar role as LFY in flower development and has limited effect on sex differentiation in papaya.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Qingyi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Hawaii Agriculture Research Center, Aiea, HI 96701, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moore</LastName><ForeName>Paul H</ForeName><Initials>PH</Initials></Author><Author ValidYN="Y"><LastName>Albert</LastName><ForeName>Henrik H</ForeName><Initials>HH</Initials></Author><Author ValidYN="Y"><LastName>Roader</LastName><ForeName>Adrienne Hk</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Ming</LastName><ForeName>Ray</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026161">MADS Domain Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029441" MajorTopicYN="N">Carica</DescriptorName><QualifierName UI="Q000235" 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