[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis)].
Identifieur interne : 003E66 ( Main/Corpus ); précédent : 003E65; suivant : 003E67[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis)].
Auteurs : Hong-Xia Qin ; Zhi-Ping Jia ; Hai-Chao Zhang ; Jing-Mei Liu ; Yu-Xia SongSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), Cold Temperature (MeSH), Droughts (MeSH), Expressed Sequence Tags (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), Stress, Physiological (MeSH), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- chemical , metabolism : Plant Proteins, Transcription Factors.
- genetics : Populus.
- metabolism : Populus.
- Amino Acid Sequence, Cloning, Molecular, Cold Temperature, Droughts, Expressed Sequence Tags, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Stress, Physiological.
Abstract
Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.
PubMed: 16468344
Links to Exploration step
pubmed:16468344Le document en format XML
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<author><name sortKey="Qin, Hong Xia" sort="Qin, Hong Xia" uniqKey="Qin H" first="Hong-Xia" last="Qin">Hong-Xia Qin</name>
<affiliation><nlm:affiliation>College of Life Science, Ningxia University, Yinchuan 750021, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jia, Zhi Ping" sort="Jia, Zhi Ping" uniqKey="Jia Z" first="Zhi-Ping" last="Jia">Zhi-Ping Jia</name>
</author>
<author><name sortKey="Zhang, Hai Chao" sort="Zhang, Hai Chao" uniqKey="Zhang H" first="Hai-Chao" last="Zhang">Hai-Chao Zhang</name>
</author>
<author><name sortKey="Liu, Jing Mei" sort="Liu, Jing Mei" uniqKey="Liu J" first="Jing-Mei" last="Liu">Jing-Mei Liu</name>
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<author><name sortKey="Song, Yu Xia" sort="Song, Yu Xia" uniqKey="Song Y" first="Yu-Xia" last="Song">Yu-Xia Song</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis)].</title>
<author><name sortKey="Qin, Hong Xia" sort="Qin, Hong Xia" uniqKey="Qin H" first="Hong-Xia" last="Qin">Hong-Xia Qin</name>
<affiliation><nlm:affiliation>College of Life Science, Ningxia University, Yinchuan 750021, China.</nlm:affiliation>
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<author><name sortKey="Jia, Zhi Ping" sort="Jia, Zhi Ping" uniqKey="Jia Z" first="Zhi-Ping" last="Jia">Zhi-Ping Jia</name>
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<author><name sortKey="Zhang, Hai Chao" sort="Zhang, Hai Chao" uniqKey="Zhang H" first="Hai-Chao" last="Zhang">Hai-Chao Zhang</name>
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<series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Cold Temperature (MeSH)</term>
<term>Droughts (MeSH)</term>
<term>Expressed Sequence Tags (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Stress, Physiological (MeSH)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
<term>Transcription Factors</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cloning, Molecular</term>
<term>Cold Temperature</term>
<term>Droughts</term>
<term>Expressed Sequence Tags</term>
<term>Gene Expression Regulation, Plant</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
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<front><div type="abstract" xml:lang="en">Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.</div>
</front>
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<DateCompleted><Year>2009</Year>
<Month>07</Month>
<Day>23</Day>
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<DateRevised><Year>2006</Year>
<Month>02</Month>
<Day>10</Day>
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<Month>Nov</Month>
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<Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title>
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<ArticleTitle>[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis)].</ArticleTitle>
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<Abstract><AbstractText>Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.</AbstractText>
</Abstract>
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<ForeName>Hong-Xia</ForeName>
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