Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.
Identifieur interne : 003E00 ( Main/Corpus ); précédent : 003D99; suivant : 003E01Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.
Auteurs : Bong Gyu Kim ; Youngshim Lee ; Hor-Gil Hur ; Yoongho Lim ; Joong-Hoon AhnSource :
- Bioscience, biotechnology, and biochemistry [ 0916-8451 ] ; 2006.
English descriptors
- KwdEn :
- Biotransformation (MeSH), Chromatography, High Pressure Liquid (MeSH), Coumarins (analysis), Coumarins (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Industrial Microbiology (MeSH), Methylation (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (enzymology), Populus (genetics), Protein O-Methyltransferase (genetics), Protein O-Methyltransferase (metabolism), Scopoletin (analysis), Scopoletin (metabolism), Umbelliferones (metabolism).
- MESH :
- chemical , analysis : Coumarins, Scopoletin.
- chemical , genetics : Plant Proteins, Protein O-Methyltransferase.
- chemical , metabolism : Coumarins, Plant Proteins, Protein O-Methyltransferase, Scopoletin, Umbelliferones.
- enzymology : Populus.
- genetics : Escherichia coli, Populus.
- metabolism : Escherichia coli.
- Biotransformation, Chromatography, High Pressure Liquid, Industrial Microbiology, Methylation.
Abstract
O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. coli expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 microM respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.
DOI: 10.1271/bbb.70.1269
PubMed: 16717435
Links to Exploration step
pubmed:16717435Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.</title><author><name sortKey="Kim, Bong Gyu" sort="Kim, Bong Gyu" uniqKey="Kim B" first="Bong Gyu" last="Kim">Bong Gyu Kim</name><affiliation><nlm:affiliation>Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Youngshim" sort="Lee, Youngshim" uniqKey="Lee Y" first="Youngshim" last="Lee">Youngshim Lee</name></author><author><name sortKey="Hur, Hor Gil" sort="Hur, Hor Gil" uniqKey="Hur H" first="Hor-Gil" last="Hur">Hor-Gil Hur</name></author><author><name sortKey="Lim, Yoongho" sort="Lim, Yoongho" uniqKey="Lim Y" first="Yoongho" last="Lim">Yoongho Lim</name></author><author><name sortKey="Ahn, Joong Hoon" sort="Ahn, Joong Hoon" uniqKey="Ahn J" first="Joong-Hoon" last="Ahn">Joong-Hoon Ahn</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16717435</idno><idno type="pmid">16717435</idno><idno type="doi">10.1271/bbb.70.1269</idno><idno type="wicri:Area/Main/Corpus">003E00</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003E00</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.</title><author><name sortKey="Kim, Bong Gyu" sort="Kim, Bong Gyu" uniqKey="Kim B" first="Bong Gyu" last="Kim">Bong Gyu Kim</name><affiliation><nlm:affiliation>Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Youngshim" sort="Lee, Youngshim" uniqKey="Lee Y" first="Youngshim" last="Lee">Youngshim Lee</name></author><author><name sortKey="Hur, Hor Gil" sort="Hur, Hor Gil" uniqKey="Hur H" first="Hor-Gil" last="Hur">Hor-Gil Hur</name></author><author><name sortKey="Lim, Yoongho" sort="Lim, Yoongho" uniqKey="Lim Y" first="Yoongho" last="Lim">Yoongho Lim</name></author><author><name sortKey="Ahn, Joong Hoon" sort="Ahn, Joong Hoon" uniqKey="Ahn J" first="Joong-Hoon" last="Ahn">Joong-Hoon Ahn</name></author></analytic><series><title level="j">Bioscience, biotechnology, and biochemistry</title><idno type="ISSN">0916-8451</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotransformation (MeSH)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Coumarins (analysis)</term><term>Coumarins (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Industrial Microbiology (MeSH)</term><term>Methylation (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Protein O-Methyltransferase (genetics)</term><term>Protein O-Methyltransferase (metabolism)</term><term>Scopoletin (analysis)</term><term>Scopoletin (metabolism)</term><term>Umbelliferones (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Coumarins</term><term>Scopoletin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Protein O-Methyltransferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Coumarins</term><term>Plant Proteins</term><term>Protein O-Methyltransferase</term><term>Scopoletin</term><term>Umbelliferones</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biotransformation</term><term>Chromatography, High Pressure Liquid</term><term>Industrial Microbiology</term><term>Methylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. coli expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 microM respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16717435</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>06</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0916-8451</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>5</Issue><PubDate><Year>2006</Year><Month>May</Month></PubDate></JournalIssue><Title>Bioscience, biotechnology, and biochemistry</Title><ISOAbbreviation>Biosci Biotechnol Biochem</ISOAbbreviation></Journal><ArticleTitle>Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar.</ArticleTitle><Pagination><MedlinePgn>1269-72</MedlinePgn></Pagination><Abstract><AbstractText>O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. coli expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 microM respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Bong Gyu</ForeName><Initials>BG</Initials><AffiliationInfo><Affiliation>Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Youngshim</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Hur</LastName><ForeName>Hor-Gil</ForeName><Initials>HG</Initials></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Yoongho</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Joong-Hoon</ForeName><Initials>JH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosci Biotechnol Biochem</MedlineTA><NlmUniqueID>9205717</NlmUniqueID><ISSNLinking>0916-8451</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003374">Coumarins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014468">Umbelliferones</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D011497">Protein O-Methyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>H5841PDT4Y</RegistryNumber><NameOfSubstance UI="C018145">scoparone</NameOfSubstance></Chemical><Chemical><RegistryNumber>KLF1HS0SXJ</RegistryNumber><NameOfSubstance UI="D012603">Scopoletin</NameOfSubstance></Chemical><Chemical><RegistryNumber>SM2XD6V944</RegistryNumber><NameOfSubstance UI="C007628">esculetin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003374" MajorTopicYN="N">Coumarins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007218" MajorTopicYN="N">Industrial Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008745" MajorTopicYN="N">Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011497" MajorTopicYN="N">Protein O-Methyltransferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012603" MajorTopicYN="N">Scopoletin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014468" MajorTopicYN="N">Umbelliferones</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>5</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>5</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16717435</ArticleId><ArticleId 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