Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.
Identifieur interne : 003A54 ( Main/Corpus ); précédent : 003A53; suivant : 003A55Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.
Auteurs : Aurore Caruso ; Françoise Chefdor ; Sabine Carpin ; Christiane Depierreux ; Francis M. Delmotte ; Guy Kahlem ; Domenico MorabitoSource :
- Journal of plant physiology [ 1618-1328 ] ; 2008.
English descriptors
- KwdEn :
- Arabidopsis (drug effects), Arabidopsis (genetics), Clone Cells (MeSH), Droughts (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Osmosis (drug effects), Plant Leaves (drug effects), Plant Leaves (genetics), Plant Leaves (growth & development), Plant Leaves (physiology), Plant Stomata (drug effects), Plant Stomata (physiology), Polyethylene Glycols (pharmacology), Populus (drug effects), Populus (genetics), Populus (physiology), RNA, Plant (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Homology, Nucleic Acid (MeSH), Stress, Physiological (drug effects), Stress, Physiological (genetics), Time Factors (MeSH), Water (MeSH).
- MESH :
- chemical , metabolism : RNA, Plant.
- chemical , pharmacology : Polyethylene Glycols.
- drug effects : Arabidopsis, Gene Expression Regulation, Plant, Osmosis, Plant Leaves, Plant Stomata, Populus, Stress, Physiological.
- genetics : Arabidopsis, Plant Leaves, Populus, Stress, Physiological.
- growth & development : Plant Leaves.
- physiology : Plant Leaves, Plant Stomata, Populus.
- Clone Cells, Droughts, Gene Expression Profiling, Genes, Plant, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Time Factors, Water.
Abstract
We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomatal conductance and a slight retardation of leaf growth, but the osmotic potential remained constant. Using the differential display technique, we searched for genes differentially expressed in response to drought at this date. Thirty-six differentially expressed leaf cDNAs were detected between stressed and control conditions. Thirty-four cDNAs clones were successfully cloned and 23 were found to share high identity with Arabidopsis thaliana and Populus trichocarpa genes. The transcriptional regulation of 21 genes was examined by reverse RNA dot blot, confirming an increase in expression for 16 of them after 10 days of treatment. Among these 16 genes, most of them are involved in a different cellular metabolic pathway. These differentially expressed genes are also involved and/or regulated by other treatments such as salt, withholding water or auxin application. The maintenance of growth observed during the first 10 days of the stress period could be due to the regulation of these genes and can be a common response between herbaceous plants and trees.
DOI: 10.1016/j.jplph.2007.04.006
PubMed: 17928100
Links to Exploration step
pubmed:17928100Le document en format XML
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Delmotte</name></author><author><name sortKey="Kahlem, Guy" sort="Kahlem, Guy" uniqKey="Kahlem G" first="Guy" last="Kahlem">Guy Kahlem</name></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:17928100</idno><idno type="pmid">17928100</idno><idno type="doi">10.1016/j.jplph.2007.04.006</idno><idno type="wicri:Area/Main/Corpus">003A54</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003A54</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.</title><author><name sortKey="Caruso, Aurore" sort="Caruso, Aurore" uniqKey="Caruso A" first="Aurore" last="Caruso">Aurore Caruso</name><affiliation><nlm:affiliation>Laboratoire de Physiologie et Biochimie Végétale, EA-2663, Université du Maine, Faculté des Sciences et Techniques, Avenue Olivier Messiaen, 72085 Le Mans Cedex 9, France.</nlm:affiliation></affiliation></author><author><name sortKey="Chefdor, Francoise" sort="Chefdor, Francoise" uniqKey="Chefdor F" first="Françoise" last="Chefdor">Françoise Chefdor</name></author><author><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name></author><author><name sortKey="Depierreux, Christiane" sort="Depierreux, Christiane" uniqKey="Depierreux C" first="Christiane" last="Depierreux">Christiane Depierreux</name></author><author><name sortKey="Delmotte, Francis M" sort="Delmotte, Francis M" uniqKey="Delmotte F" first="Francis M" last="Delmotte">Francis M. Delmotte</name></author><author><name sortKey="Kahlem, Guy" sort="Kahlem, Guy" uniqKey="Kahlem G" first="Guy" last="Kahlem">Guy Kahlem</name></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (drug effects)</term><term>Arabidopsis (genetics)</term><term>Clone Cells (MeSH)</term><term>Droughts (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Osmosis (drug effects)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (physiology)</term><term>Plant Stomata (drug effects)</term><term>Plant Stomata (physiology)</term><term>Polyethylene Glycols (pharmacology)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (physiology)</term><term>RNA, Plant (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Stress, Physiological (drug effects)</term><term>Stress, Physiological (genetics)</term><term>Time Factors (MeSH)</term><term>Water (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Polyethylene Glycols</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Gene Expression Regulation, Plant</term><term>Osmosis</term><term>Plant Leaves</term><term>Plant Stomata</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Plant Leaves</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term><term>Plant Stomata</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term><term>Droughts</term><term>Gene Expression Profiling</term><term>Genes, Plant</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Homology, Nucleic Acid</term><term>Time Factors</term><term>Water</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomatal conductance and a slight retardation of leaf growth, but the osmotic potential remained constant. Using the differential display technique, we searched for genes differentially expressed in response to drought at this date. Thirty-six differentially expressed leaf cDNAs were detected between stressed and control conditions. Thirty-four cDNAs clones were successfully cloned and 23 were found to share high identity with Arabidopsis thaliana and Populus trichocarpa genes. The transcriptional regulation of 21 genes was examined by reverse RNA dot blot, confirming an increase in expression for 16 of them after 10 days of treatment. Among these 16 genes, most of them are involved in a different cellular metabolic pathway. These differentially expressed genes are also involved and/or regulated by other treatments such as salt, withholding water or auxin application. The maintenance of growth observed during the first 10 days of the stress period could be due to the regulation of these genes and can be a common response between herbaceous plants and trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17928100</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>22</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>165</Volume><Issue>9</Issue><PubDate><Year>2008</Year><Month>Jun</Month><Day>16</Day></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.</ArticleTitle><Pagination><MedlinePgn>932-41</MedlinePgn></Pagination><Abstract><AbstractText>We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomatal conductance and a slight retardation of leaf growth, but the osmotic potential remained constant. Using the differential display technique, we searched for genes differentially expressed in response to drought at this date. Thirty-six differentially expressed leaf cDNAs were detected between stressed and control conditions. Thirty-four cDNAs clones were successfully cloned and 23 were found to share high identity with Arabidopsis thaliana and Populus trichocarpa genes. The transcriptional regulation of 21 genes was examined by reverse RNA dot blot, confirming an increase in expression for 16 of them after 10 days of treatment. Among these 16 genes, most of them are involved in a different cellular metabolic pathway. These differentially expressed genes are also involved and/or regulated by other treatments such as salt, withholding water or auxin application. The maintenance of growth observed during the first 10 days of the stress period could be due to the regulation of these genes and can be a common response between herbaceous plants and trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Caruso</LastName><ForeName>Aurore</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Physiologie et Biochimie Végétale, EA-2663, Université du Maine, Faculté des Sciences et Techniques, Avenue Olivier Messiaen, 72085 Le Mans Cedex 9, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chefdor</LastName><ForeName>Françoise</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Carpin</LastName><ForeName>Sabine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Depierreux</LastName><ForeName>Christiane</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Delmotte</LastName><ForeName>Francis M</ForeName><Initials>FM</Initials></Author><Author ValidYN="Y"><LastName>Kahlem</LastName><ForeName>Guy</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Morabito</LastName><ForeName>Domenico</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>10</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical><Chemical><RegistryNumber>3WJQ0SDW1A</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" 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PubStatus="entrez"><Year>2007</Year><Month>10</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17928100</ArticleId><ArticleId IdType="pii">S0176-1617(07)00219-2</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2007.04.006</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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