A modified T-vector for simplified assembly of hairpin RNAi constructs.
Identifieur interne : 003940 ( Main/Corpus ); précédent : 003939; suivant : 003941A modified T-vector for simplified assembly of hairpin RNAi constructs.
Auteurs : Keming Luo ; Scott A. Harding ; Chung-Jui TsaiSource :
- Biotechnology letters [ 0141-5492 ] ; 2008.
English descriptors
- KwdEn :
- Alcohol Oxidoreductases (genetics), Cloning, Molecular (methods), Genes, Reporter (genetics), Genetic Vectors (genetics), Green Fluorescent Proteins (genetics), Plant Proteins (genetics), Populus (genetics), RNA Interference (MeSH), RNA, Plant (genetics), Repetitive Sequences, Nucleic Acid (genetics).
- MESH :
- chemical , genetics : Alcohol Oxidoreductases, Green Fluorescent Proteins, Plant Proteins, RNA, Plant.
- genetics : Genes, Reporter, Genetic Vectors, Populus, Repetitive Sequences, Nucleic Acid.
- methods : Cloning, Molecular.
- RNA Interference.
Abstract
We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.
DOI: 10.1007/s10529-008-9673-x
PubMed: 18317701
Links to Exploration step
pubmed:18317701Le document en format XML
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<author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name>
<affiliation><nlm:affiliation>Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.</nlm:affiliation>
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<author><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name>
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<author><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name>
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<author><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name>
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<term>Genetic Vectors (genetics)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Populus (genetics)</term>
<term>RNA Interference (MeSH)</term>
<term>RNA, Plant (genetics)</term>
<term>Repetitive Sequences, Nucleic Acid (genetics)</term>
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<term>Repetitive Sequences, Nucleic Acid</term>
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<front><div type="abstract" xml:lang="en">We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.</div>
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<Title>Biotechnology letters</Title>
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<Abstract><AbstractText>We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.</AbstractText>
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