Immunolocalization of (1,4)-beta-galactan in tension wood fibers of poplar.
Identifieur interne : 003872 ( Main/Corpus ); précédent : 003871; suivant : 003873Immunolocalization of (1,4)-beta-galactan in tension wood fibers of poplar.
Auteurs : Matthias ArendSource :
- Tree physiology [ 0829-318X ] ; 2008.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (MeSH), Cell Wall (chemistry), Cell Wall (ultrastructure), Galactans (analysis), Immunohistochemistry (methods), Microscopy, Electron, Transmission (MeSH), Microscopy, Fluorescence (MeSH), Populus (chemistry), Populus (growth & development), Populus (ultrastructure), Wood (chemistry), Wood (growth & development), Wood (ultrastructure).
- MESH :
- chemical , analysis : Galactans.
- chemical : Antibodies, Monoclonal.
- chemistry : Cell Wall, Populus, Wood.
- growth & development : Populus, Wood.
- methods : Immunohistochemistry.
- ultrastructure : Cell Wall, Populus, Wood.
- Microscopy, Electron, Transmission, Microscopy, Fluorescence.
Abstract
The occurrence and distribution of (1,4)-beta-galactan in wood cells of poplar (Populus trichocarpa Torr. & A. Gray x P. koreana Rehder) were studied by immunolabeling with a monoclonal antibody (LM5) specific to this carbohydrate epitope. Immunofluorescence microscopy revealed exclusive binding of the LM5 antibody to tension wood fibers, indicating the specific presence of (1,4)-beta-galactan in cell walls of this wood cell type. Higher magnifications achieved with the fluorescence microscope and additional immunogold electron microscopy showed that the binding of the LM5 antibody was mainly restricted to a narrow cell wall area between the gelatinous G-layer and the secondary cell wall. This labeling pattern strongly suggests a role of (1,4)-beta-galactan in cross-linking the G-layer and secondary cell wall. Furthermore, the exclusive localization of (1,4)-beta-galactan in tension wood strengthens the view that this carbohydrate epitope can be considered a highly specific marker of reaction wood formation in mechanically stressed trees.
DOI: 10.1093/treephys/28.8.1263
PubMed: 18519257
Links to Exploration step
pubmed:18519257Le document en format XML
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<author><name sortKey="Arend, Matthias" sort="Arend, Matthias" uniqKey="Arend M" first="Matthias" last="Arend">Matthias Arend</name>
<affiliation><nlm:affiliation>Technische Universität München, Fachgebiet Angewandte Holzbiologie, Winzererstrasse 45, München, Germany. arend@wzw.tum.de</nlm:affiliation>
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<series><title level="j">Tree physiology</title>
<idno type="ISSN">0829-318X</idno>
<imprint><date when="2008" type="published">2008</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (MeSH)</term>
<term>Cell Wall (chemistry)</term>
<term>Cell Wall (ultrastructure)</term>
<term>Galactans (analysis)</term>
<term>Immunohistochemistry (methods)</term>
<term>Microscopy, Electron, Transmission (MeSH)</term>
<term>Microscopy, Fluorescence (MeSH)</term>
<term>Populus (chemistry)</term>
<term>Populus (growth & development)</term>
<term>Populus (ultrastructure)</term>
<term>Wood (chemistry)</term>
<term>Wood (growth & development)</term>
<term>Wood (ultrastructure)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Galactans</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term>
<term>Populus</term>
<term>Wood</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term>
<term>Wood</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunohistochemistry</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Wall</term>
<term>Populus</term>
<term>Wood</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Transmission</term>
<term>Microscopy, Fluorescence</term>
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<front><div type="abstract" xml:lang="en">The occurrence and distribution of (1,4)-beta-galactan in wood cells of poplar (Populus trichocarpa Torr. & A. Gray x P. koreana Rehder) were studied by immunolabeling with a monoclonal antibody (LM5) specific to this carbohydrate epitope. Immunofluorescence microscopy revealed exclusive binding of the LM5 antibody to tension wood fibers, indicating the specific presence of (1,4)-beta-galactan in cell walls of this wood cell type. Higher magnifications achieved with the fluorescence microscope and additional immunogold electron microscopy showed that the binding of the LM5 antibody was mainly restricted to a narrow cell wall area between the gelatinous G-layer and the secondary cell wall. This labeling pattern strongly suggests a role of (1,4)-beta-galactan in cross-linking the G-layer and secondary cell wall. Furthermore, the exclusive localization of (1,4)-beta-galactan in tension wood strengthens the view that this carbohydrate epitope can be considered a highly specific marker of reaction wood formation in mechanically stressed trees.</div>
</front>
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<DateCompleted><Year>2008</Year>
<Month>09</Month>
<Day>18</Day>
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<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>28</Volume>
<Issue>8</Issue>
<PubDate><Year>2008</Year>
<Month>Aug</Month>
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<Title>Tree physiology</Title>
<ISOAbbreviation>Tree Physiol</ISOAbbreviation>
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<ArticleTitle>Immunolocalization of (1,4)-beta-galactan in tension wood fibers of poplar.</ArticleTitle>
<Pagination><MedlinePgn>1263-7</MedlinePgn>
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<Abstract><AbstractText>The occurrence and distribution of (1,4)-beta-galactan in wood cells of poplar (Populus trichocarpa Torr. & A. Gray x P. koreana Rehder) were studied by immunolabeling with a monoclonal antibody (LM5) specific to this carbohydrate epitope. Immunofluorescence microscopy revealed exclusive binding of the LM5 antibody to tension wood fibers, indicating the specific presence of (1,4)-beta-galactan in cell walls of this wood cell type. Higher magnifications achieved with the fluorescence microscope and additional immunogold electron microscopy showed that the binding of the LM5 antibody was mainly restricted to a narrow cell wall area between the gelatinous G-layer and the secondary cell wall. This labeling pattern strongly suggests a role of (1,4)-beta-galactan in cross-linking the G-layer and secondary cell wall. Furthermore, the exclusive localization of (1,4)-beta-galactan in tension wood strengthens the view that this carbohydrate epitope can be considered a highly specific marker of reaction wood formation in mechanically stressed trees.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arend</LastName>
<ForeName>Matthias</ForeName>
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<AffiliationInfo><Affiliation>Technische Universität München, Fachgebiet Angewandte Holzbiologie, Winzererstrasse 45, München, Germany. arend@wzw.tum.de</Affiliation>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C411147">1,4-beta-galactan</NameOfSubstance>
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<MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName>
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<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
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<MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName>
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