Over-expression of bacterial gamma-glutamylcysteine synthetase (GSH1) in plastids affects photosynthesis, growth and sulphur metabolism in poplar (Populus tremula x Populus alba) dependent on the resulting gamma-glutamylcysteine and glutathione levels.
Identifieur interne : 003282 ( Main/Corpus ); précédent : 003281; suivant : 003283Over-expression of bacterial gamma-glutamylcysteine synthetase (GSH1) in plastids affects photosynthesis, growth and sulphur metabolism in poplar (Populus tremula x Populus alba) dependent on the resulting gamma-glutamylcysteine and glutathione levels.
Auteurs : Cornelia Herschbach ; Luca Rizzini ; Susanne Mult ; Tanja Hartmann ; Florian Busch ; Andreas D. Peuke ; Stanislav Kopriva ; Ingo EnsmingerSource :
- Plant, cell & environment [ 1365-3040 ] ; 2010.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Dipeptides (analysis), Escherichia coli (enzymology), Glutamate-Cysteine Ligase (metabolism), Glutathione (analysis), Phloem (chemistry), Photosynthesis (MeSH), Photosystem II Protein Complex (metabolism), Plant Leaves (chemistry), Plant Roots (chemistry), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Plastids (genetics), Plastids (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism), Sulfur Compounds (metabolism), Transgenes (MeSH).
- MESH :
- chemical , analysis : Dipeptides, Glutathione.
- chemical , genetics : Bacterial Proteins.
- chemical , metabolism : Bacterial Proteins, Glutamate-Cysteine Ligase, Photosystem II Protein Complex, Sulfur Compounds.
- chemistry : Phloem, Plant Leaves, Plant Roots.
- enzymology : Escherichia coli.
- genetics : Plants, Genetically Modified, Plastids, Populus.
- growth & development : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Plastids, Populus.
- Photosynthesis, Transgenes.
Abstract
We compared three transgenic poplar lines over-expressing the bacterial gamma-glutamylcysteine synthetase (GSH1) targeted to plastids. Lines Lggs6 and Lggs12 have two copies, while line Lggs20 has three copies of the transgene. The three lines differ in their expression levels of the transgene and in the accumulation of gamma-glutamylcysteine (gamma-EC) and glutathione (GSH) in leaves, roots and phloem exudates. The lowest transgene expression level was observed in line Lggs6 which showed an increased growth, an enhanced rate of photosynthesis and a decreased excitation pressure (1-qP). The latter typically represents a lower reduction state of the plastoquinone pool, and thereby facilitates electron flow along the electron transport chain. Line Lggs12 showed the highest transgene expression level, highest gamma-EC accumulation in leaves and highest GSH enrichment in phloem exudates and roots. This line also exhibited a reduced growth, and after a prolonged growth of 4.5 months, symptoms of leaf injury. Decreased maximum quantum yield (F(v)/F(m)) indicated down-regulation of photosystem II reaction centre (PSII RC), which correlates with decreased PSII RC protein D1 (PsbA) and diminished light-harvesting complex (Lhcb1). Potential effects of changes in chloroplastic and cytosolic GSH contents on photosynthesis, growth and the whole-plant sulphur nutrition are discussed for each line.
DOI: 10.1111/j.1365-3040.2010.02135.x
PubMed: 20199621
Links to Exploration step
pubmed:20199621Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Over-expression of bacterial gamma-glutamylcysteine synthetase (GSH1) in plastids affects photosynthesis, growth and sulphur metabolism in poplar (Populus tremula x Populus alba) dependent on the resulting gamma-glutamylcysteine and glutathione levels.</title><author><name sortKey="Herschbach, Cornelia" sort="Herschbach, Cornelia" uniqKey="Herschbach C" first="Cornelia" last="Herschbach">Cornelia Herschbach</name><affiliation><nlm:affiliation>Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee 053/054, D-79110 Freiburg i.B., Germany. cornelia.herschbach@ctp.uni-freiburg.de</nlm:affiliation></affiliation></author><author><name sortKey="Rizzini, Luca" sort="Rizzini, Luca" uniqKey="Rizzini L" first="Luca" last="Rizzini">Luca Rizzini</name></author><author><name sortKey="Mult, Susanne" sort="Mult, Susanne" uniqKey="Mult S" first="Susanne" last="Mult">Susanne Mult</name></author><author><name sortKey="Hartmann, Tanja" sort="Hartmann, Tanja" uniqKey="Hartmann T" first="Tanja" last="Hartmann">Tanja Hartmann</name></author><author><name sortKey="Busch, Florian" sort="Busch, Florian" uniqKey="Busch F" first="Florian" last="Busch">Florian Busch</name></author><author><name sortKey="Peuke, Andreas D" sort="Peuke, Andreas D" uniqKey="Peuke A" first="Andreas D" last="Peuke">Andreas D. Peuke</name></author><author><name sortKey="Kopriva, Stanislav" sort="Kopriva, Stanislav" uniqKey="Kopriva S" first="Stanislav" last="Kopriva">Stanislav Kopriva</name></author><author><name sortKey="Ensminger, Ingo" sort="Ensminger, Ingo" uniqKey="Ensminger I" first="Ingo" last="Ensminger">Ingo Ensminger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20199621</idno><idno type="pmid">20199621</idno><idno type="doi">10.1111/j.1365-3040.2010.02135.x</idno><idno type="wicri:Area/Main/Corpus">003282</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003282</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Over-expression of bacterial gamma-glutamylcysteine synthetase (GSH1) in plastids affects photosynthesis, growth and sulphur metabolism in poplar (Populus tremula x Populus alba) dependent on the resulting gamma-glutamylcysteine and glutathione levels.</title><author><name sortKey="Herschbach, Cornelia" sort="Herschbach, Cornelia" uniqKey="Herschbach C" first="Cornelia" last="Herschbach">Cornelia Herschbach</name><affiliation><nlm:affiliation>Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee 053/054, D-79110 Freiburg i.B., Germany. cornelia.herschbach@ctp.uni-freiburg.de</nlm:affiliation></affiliation></author><author><name sortKey="Rizzini, Luca" sort="Rizzini, Luca" uniqKey="Rizzini L" first="Luca" last="Rizzini">Luca Rizzini</name></author><author><name sortKey="Mult, Susanne" sort="Mult, Susanne" uniqKey="Mult S" first="Susanne" last="Mult">Susanne Mult</name></author><author><name sortKey="Hartmann, Tanja" sort="Hartmann, Tanja" uniqKey="Hartmann T" first="Tanja" last="Hartmann">Tanja Hartmann</name></author><author><name sortKey="Busch, Florian" sort="Busch, Florian" uniqKey="Busch F" first="Florian" last="Busch">Florian Busch</name></author><author><name sortKey="Peuke, Andreas D" sort="Peuke, Andreas D" uniqKey="Peuke A" first="Andreas D" last="Peuke">Andreas D. Peuke</name></author><author><name sortKey="Kopriva, Stanislav" sort="Kopriva, Stanislav" uniqKey="Kopriva S" first="Stanislav" last="Kopriva">Stanislav Kopriva</name></author><author><name sortKey="Ensminger, Ingo" sort="Ensminger, Ingo" uniqKey="Ensminger I" first="Ingo" last="Ensminger">Ingo Ensminger</name></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Dipeptides (analysis)</term><term>Escherichia coli (enzymology)</term><term>Glutamate-Cysteine Ligase (metabolism)</term><term>Glutathione (analysis)</term><term>Phloem (chemistry)</term><term>Photosynthesis (MeSH)</term><term>Photosystem II Protein Complex (metabolism)</term><term>Plant Leaves (chemistry)</term><term>Plant Roots (chemistry)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plastids (genetics)</term><term>Plastids (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Sulfur Compounds (metabolism)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Dipeptides</term><term>Glutathione</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Glutamate-Cysteine Ligase</term><term>Photosystem II Protein Complex</term><term>Sulfur Compounds</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phloem</term><term>Plant Leaves</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Plastids</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Plastids</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Photosynthesis</term><term>Transgenes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We compared three transgenic poplar lines over-expressing the bacterial gamma-glutamylcysteine synthetase (GSH1) targeted to plastids. Lines Lggs6 and Lggs12 have two copies, while line Lggs20 has three copies of the transgene. The three lines differ in their expression levels of the transgene and in the accumulation of gamma-glutamylcysteine (gamma-EC) and glutathione (GSH) in leaves, roots and phloem exudates. The lowest transgene expression level was observed in line Lggs6 which showed an increased growth, an enhanced rate of photosynthesis and a decreased excitation pressure (1-qP). The latter typically represents a lower reduction state of the plastoquinone pool, and thereby facilitates electron flow along the electron transport chain. Line Lggs12 showed the highest transgene expression level, highest gamma-EC accumulation in leaves and highest GSH enrichment in phloem exudates and roots. This line also exhibited a reduced growth, and after a prolonged growth of 4.5 months, symptoms of leaf injury. Decreased maximum quantum yield (F(v)/F(m)) indicated down-regulation of photosystem II reaction centre (PSII RC), which correlates with decreased PSII RC protein D1 (PsbA) and diminished light-harvesting complex (Lhcb1). Potential effects of changes in chloroplastic and cytosolic GSH contents on photosynthesis, growth and the whole-plant sulphur nutrition are discussed for each line.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20199621</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>13</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>7</Issue><PubDate><Year>2010</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>Over-expression of bacterial gamma-glutamylcysteine synthetase (GSH1) in plastids affects photosynthesis, growth and sulphur metabolism in poplar (Populus tremula x Populus alba) dependent on the resulting gamma-glutamylcysteine and glutathione levels.</ArticleTitle><Pagination><MedlinePgn>1138-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-3040.2010.02135.x</ELocationID><Abstract><AbstractText>We compared three transgenic poplar lines over-expressing the bacterial gamma-glutamylcysteine synthetase (GSH1) targeted to plastids. Lines Lggs6 and Lggs12 have two copies, while line Lggs20 has three copies of the transgene. The three lines differ in their expression levels of the transgene and in the accumulation of gamma-glutamylcysteine (gamma-EC) and glutathione (GSH) in leaves, roots and phloem exudates. The lowest transgene expression level was observed in line Lggs6 which showed an increased growth, an enhanced rate of photosynthesis and a decreased excitation pressure (1-qP). The latter typically represents a lower reduction state of the plastoquinone pool, and thereby facilitates electron flow along the electron transport chain. Line Lggs12 showed the highest transgene expression level, highest gamma-EC accumulation in leaves and highest GSH enrichment in phloem exudates and roots. This line also exhibited a reduced growth, and after a prolonged growth of 4.5 months, symptoms of leaf injury. Decreased maximum quantum yield (F(v)/F(m)) indicated down-regulation of photosystem II reaction centre (PSII RC), which correlates with decreased PSII RC protein D1 (PsbA) and diminished light-harvesting complex (Lhcb1). Potential effects of changes in chloroplastic and cytosolic GSH contents on photosynthesis, growth and the whole-plant sulphur nutrition are discussed for each line.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Herschbach</LastName><ForeName>Cornelia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee 053/054, D-79110 Freiburg i.B., Germany. cornelia.herschbach@ctp.uni-freiburg.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rizzini</LastName><ForeName>Luca</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Mult</LastName><ForeName>Susanne</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hartmann</LastName><ForeName>Tanja</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Busch</LastName><ForeName>Florian</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Peuke</LastName><ForeName>Andreas D</ForeName><Initials>AD</Initials></Author><Author ValidYN="Y"><LastName>Kopriva</LastName><ForeName>Stanislav</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ensminger</LastName><ForeName>Ingo</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>03</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004151">Dipeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045332">Photosystem II Protein Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013457">Sulfur Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.2.2</RegistryNumber><NameOfSubstance UI="D005721">Glutamate-Cysteine Ligase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical><Chemical><RegistryNumber>M984VJS48P</RegistryNumber><NameOfSubstance UI="C017341">gamma-glutamylcysteine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004151" MajorTopicYN="N">Dipeptides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005721" MajorTopicYN="N">Glutamate-Cysteine Ligase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052585" MajorTopicYN="N">Phloem</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="Y">Photosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045332" MajorTopicYN="N">Photosystem II Protein Complex</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018087" MajorTopicYN="N">Plastids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013457" MajorTopicYN="N">Sulfur Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>10</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20199621</ArticleId><ArticleId IdType="pii">PCE2135</ArticleId><ArticleId IdType="doi">10.1111/j.1365-3040.2010.02135.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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