Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.
Identifieur interne : 003256 ( Main/Corpus ); précédent : 003255; suivant : 003257Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.
Auteurs : M. Bonadei ; C. Calvio ; D. Carbonera ; A. Galizzi ; E. Quattrini ; A. BalestrazziSource :
- Folia microbiologica [ 1874-9356 ] ; 2010.
English descriptors
- KwdEn :
- Aerobiosis (MeSH), Cluster Analysis (MeSH), Colony Count, Microbial (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Endospore-Forming Bacteria (classification), Endospore-Forming Bacteria (isolation & purification), Endospore-Forming Bacteria (physiology), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plants, Genetically Modified (microbiology), Populus (microbiology), RNA, Ribosomal, 16S (genetics), Seasons (MeSH), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Ribosomal.
- chemical , genetics : DNA, Bacterial, DNA, Ribosomal, RNA, Ribosomal, 16S.
- classification : Endospore-Forming Bacteria.
- isolation & purification : Endospore-Forming Bacteria.
- microbiology : Plants, Genetically Modified, Populus.
- physiology : Endospore-Forming Bacteria.
- Aerobiosis, Cluster Analysis, Colony Count, Microbial, Molecular Sequence Data, Phylogeny, Seasons, Sequence Analysis, DNA, Soil Microbiology.
Abstract
The impact of transgenic white poplars (Populus alba L. cv. 'Villafranca') was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations in the range of 10(6)-2.5 x 10(8) CFU/g dry soil and of 10(4)-5 x 10(8) CFU/g dry soil, respectively. Changes occurred also within the culturable SFB population with size varying at 10(3)-5 x 10(4) CFU/g dry soil and 10(2)-2 x 10(5) CFU/g dry soil in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.
DOI: 10.1007/s12223-010-0007-8
PubMed: 20336503
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pubmed:20336503Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.</title><author><name sortKey="Bonadei, M" sort="Bonadei, M" uniqKey="Bonadei M" first="M" last="Bonadei">M. Bonadei</name><affiliation><nlm:affiliation>Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Calvio, C" sort="Calvio, C" uniqKey="Calvio C" first="C" last="Calvio">C. Calvio</name></author><author><name sortKey="Carbonera, D" sort="Carbonera, D" uniqKey="Carbonera D" first="D" last="Carbonera">D. Carbonera</name></author><author><name sortKey="Galizzi, A" sort="Galizzi, A" uniqKey="Galizzi A" first="A" last="Galizzi">A. Galizzi</name></author><author><name sortKey="Quattrini, E" sort="Quattrini, E" uniqKey="Quattrini E" first="E" last="Quattrini">E. Quattrini</name></author><author><name sortKey="Balestrazzi, A" sort="Balestrazzi, A" uniqKey="Balestrazzi A" first="A" last="Balestrazzi">A. Balestrazzi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20336503</idno><idno type="pmid">20336503</idno><idno type="doi">10.1007/s12223-010-0007-8</idno><idno type="wicri:Area/Main/Corpus">003256</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003256</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.</title><author><name sortKey="Bonadei, M" sort="Bonadei, M" uniqKey="Bonadei M" first="M" last="Bonadei">M. Bonadei</name><affiliation><nlm:affiliation>Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Calvio, C" sort="Calvio, C" uniqKey="Calvio C" first="C" last="Calvio">C. Calvio</name></author><author><name sortKey="Carbonera, D" sort="Carbonera, D" uniqKey="Carbonera D" first="D" last="Carbonera">D. Carbonera</name></author><author><name sortKey="Galizzi, A" sort="Galizzi, A" uniqKey="Galizzi A" first="A" last="Galizzi">A. Galizzi</name></author><author><name sortKey="Quattrini, E" sort="Quattrini, E" uniqKey="Quattrini E" first="E" last="Quattrini">E. Quattrini</name></author><author><name sortKey="Balestrazzi, A" sort="Balestrazzi, A" uniqKey="Balestrazzi A" first="A" last="Balestrazzi">A. Balestrazzi</name></author></analytic><series><title level="j">Folia microbiologica</title><idno type="eISSN">1874-9356</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerobiosis (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>Colony Count, Microbial (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Endospore-Forming Bacteria (classification)</term><term>Endospore-Forming Bacteria (isolation & purification)</term><term>Endospore-Forming Bacteria (physiology)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plants, Genetically Modified (microbiology)</term><term>Populus (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Seasons (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Endospore-Forming Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Endospore-Forming Bacteria</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Endospore-Forming Bacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aerobiosis</term><term>Cluster Analysis</term><term>Colony Count, Microbial</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Seasons</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The impact of transgenic white poplars (Populus alba L. cv. 'Villafranca') was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations in the range of 10(6)-2.5 x 10(8) CFU/g dry soil and of 10(4)-5 x 10(8) CFU/g dry soil, respectively. Changes occurred also within the culturable SFB population with size varying at 10(3)-5 x 10(4) CFU/g dry soil and 10(2)-2 x 10(5) CFU/g dry soil in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20336503</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1874-9356</ISSN><JournalIssue CitedMedium="Internet"><Volume>55</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Folia microbiologica</Title><ISOAbbreviation>Folia Microbiol (Praha)</ISOAbbreviation></Journal><ArticleTitle>Spore-forming bacteria in soil cultivated with GM white poplars: isolation and characterization.</ArticleTitle><Pagination><MedlinePgn>39-46</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12223-010-0007-8</ELocationID><Abstract><AbstractText>The impact of transgenic white poplars (Populus alba L. cv. 'Villafranca') was assessed on the soil aerobic spore-forming bacteria (SFB). The genetically modified poplars, expressing either the StSy gene for resveratrol production or the bar gene for herbicide tolerance, were cultivated in greenhouse. The occurrence of SFB was monitored in soil samples collected at eight different timepoints over a two-year period. The total culturable bacterial population of the StSy and bar trials underwent significant seasonal fluctuations in the range of 10(6)-2.5 x 10(8) CFU/g dry soil and of 10(4)-5 x 10(8) CFU/g dry soil, respectively. Changes occurred also within the culturable SFB population with size varying at 10(3)-5 x 10(4) CFU/g dry soil and 10(2)-2 x 10(5) CFU/g dry soil in the StSy and bar trials, respectively. No significant differences in the size of the total and SFB culturable populations were observed when comparing each transgenic line with the nontransformed control line while seasonal shifts of soil bacterial populations were evident in both trials. The culturable SFB fraction included three isolates (SFB-1, SFB-2 and SFB-3) classified by 16S rDNA sequence analysis as members of the Bacillus genus. According to the reported data, cultivation of both herbicide-resistant and resveratrol-producing GM white poplars did not affect the culturable SFB population at the soil level.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bonadei</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Calvio</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Carbonera</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Galizzi</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Quattrini</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author 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